• Title/Summary/Keyword: infection experiments

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Significance of Semame Seedborne Fungi, with special Reference to Corynespora cassiicola (참깨의 종자전염성 진균과 그 병원성 : Corynespora cussiicola를 중심으로)

  • Yu Seung-Heon
    • Korean journal of applied entomology
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    • v.20 no.4 s.49
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    • pp.183-190
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    • 1981
  • Alternaria sesami, A. sesamicola, A. tenuis, A. longissima, Cercospora sesami, Cephalosporium sp., Corynespora cassiicola, Fusarium equiseti, F. moniliforme, F. oxysporum, F. semitectum, Macrophomina phaseolina and Myrothecium roridum were detected from 40 seed samples of sesame. A sesami, A. sesamicola, A. tenuis and C. cassiicola were the predominant fungi. Except C. cassiicola, all fungi were almost completly reduced and wiped out the infection by pretreatment with chlorine. Plating components also indicate that C. cassiicola was well-established infections. Seedborne infection of C. cussiicola caused heavy seed rot and seedling mortality. Detailed description has been given on the habit character of C. cassiicola under stereoscopic microscope and the variation in colony character and spore morphology have been taken into account. In inoculation experiments, C. cassiicola produced severe leaf and stem spots and blights on sesame plants resulted in ultimate death of the plants. A. sesami, A. sesamicola A. longissima and C. sesami also produced mild to severe leaf spotting and leaf blight when suspension of their conidia were sprayed on to plants. In soil inoculation experiments, F. oxysporum and M. phaseolina were the most pathogenic causing seed rot and seedling blight.

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Yield Loss of Spring Chinese Cabbage as Affected by Infection Time of Clubroot Disease in Fields (봄배추 무사마귀병의 포장 감염시기와 피해)

  • 김충회;조원대;김홍모
    • Research in Plant Disease
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    • v.6 no.1
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    • pp.23-26
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    • 2000
  • Effects of infection time of the clubroot pathogen, Plasmodiophora brassicae, on yield loss of Spring-sown Chinese cabbage plants were examined in field experiments. Yield loss of Chinese cabbage plants increased as the infection time becomes early. Plants infected at 20 days after transplanting or earlier were completely killed before harvest, and those infected at 30 days after transplanting were healthy in appearance but their head weights were reduced to 59% with poor commodity value. The plants infected 40 days after transplanting were not affected in yield. Development of root hairs in diseased plants was greatly reduced as the infection progressed, and root length was reduced to 1/2 to 1/3 of that of healthy plants. root galls were first developed 20 days after inoculation and rapidly enlarged to reach the peak in size 20 days from initial development, and decayed thereafter. Development and decay of root galls tended to be faster at later season as air temperature became high, regardless of the infection time. Diseased plants started to wilt approximately 10 days after root gall development. Root galls began to decay 10 days after initial plant wilting, and then were completely rotten within following 10 days. Based on the results, root gall development stages on spring-sown Chinese cabbage plants could be grouped into 20 days of root gall enlargement period, and 10 days of root gall decay period, followed by survival period in soil.

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Drug Target Identification and Elucidation of Natural Inhibitors for Bordetella petrii: An In Silico Study

  • Rath, Surya Narayan;Ray, Manisha;Pattnaik, Animesh;Pradhan, Sukanta Kumar
    • Genomics & Informatics
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    • v.14 no.4
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    • pp.241-254
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    • 2016
  • Environmental microbes like Bordetella petrii has been established as a causative agent for various infectious diseases in human. Again, development of drug resistance in B. petrii challenged to combat against the infection. Identification of potential drug target and proposing a novel lead compound against the pathogen has a great aid and value. In this study, bioinformatics tools and technology have been applied to suggest a potential drug target by screening the proteome information of B. petrii DSM 12804 (accession No. PRJNA28135) from genome database of National Centre for Biotechnology information. In this regards, the inhibitory effect of nine natural compounds like ajoene (Allium sativum), allicin (A. sativum), cinnamaldehyde (Cinnamomum cassia), curcumin (Curcuma longa), gallotannin (active component of green tea and red wine), isoorientin (Anthopterus wardii), isovitexin (A. wardii), neral (Melissa officinalis), and vitexin (A. wardii) have been acknowledged with anti-bacterial properties and hence tested against identified drug target of B. petrii by implicating computational approach. The in silico studies revealed the hypothesis that lpxD could be a potential drug target and with recommendation of a strong inhibitory effect of selected natural compounds against infection caused due to B. petrii, would be further validated through in vitro experiments.

Immunoactivity of Ginsenosides Re and Rg1 that Enhances Resistance of Mice Against Experimental Disseminated Candidiasis

  • Han, Yong-Moon;Jin, Byung-Suk;Ko, Sung-Kwon;Lee, Jue-Hee
    • Natural Product Sciences
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    • v.10 no.3
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    • pp.134-139
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    • 2004
  • In this study, an immunoactivity of panaxtriol ginsenosides Re and Rg1 against infection due to Candida albicans was investigated. The ginsenosides were extracted from Red Ginseng with 85% ethanol and heat-treatment and were analyzed by HPLC on water-acetonitrile as a mobile phase. The HPLC analysis revealed that the extract contained ginsenosides Re and Rg1, which were eluted as a combined peak. By agar diffusion susceptibility, the mixture of Re and Rg1 had no growth-inhibitory activity on C. albicans yeast cells. However, in animal tests BALB/c mice given the mixture of Re and Rg1 intraperitoneally (Lp.) before intravenous (Lv.) infection with live C. albicans yeast cells had longer mean survival times (MST) than MST of control mice groups that received only buffer solution instead of Re and Rg1. In experiments 60% of the ginsenosides-treated mice survived the entire duration of the 50-day observation. The Re and Rg1 mixture induced production of nitric oxide when interacted with RAW 264.7 macrophage cell line. In addition, the mixture caused morphological change of the macrophages. These data indicate that immunostimulation by the Re and Rg1 may be responsible for the protection of mice against disseminated candidiasis.

The Story of Serratia Marcescens: Pathologic Risk Factors in Breast Implant Surgery

  • Yao, Caroline A.;Wang, Diana;Kulber, David A.
    • Archives of Plastic Surgery
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    • v.41 no.4
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    • pp.414-417
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    • 2014
  • Serratia marcescens (S. marcescens) emerged as an opportunist in the setting of immunodeficiency in the 1970s, when serious infections occurred in San Francisco hospitals after USA. Navy experiments had aerosolized the bacteria to study biologic warfare. We investigate the risks of S. marcescens in San Franciscans who undergo mastectomy with implant reconstruction. From 2007 to 2011, the senior author took breast capsule cultures for all patients at the time of tissue expander exchange/explant. Of the 142 women who had reconstruction, 23 had positive cultures. Only the two patients who were positive for S. marcescens developed clinical infections that required explantation. Both had postoperative chemotherapy with transient neutropenia, and both had close ties to San Francisco. Clinical signs of infection emerged for both patients months after initial surgery, despite having previously well healed incisions. Other patients were culture positive for Pseudomonas, Proteus, Enterococcus and MRSA and did not develop require explant. While the link between San Francisco and S. marcescens is controversial, a patient's geography is a simple screening tool when considering postoperative risks, especially in the immunocompromised. Closer monitoring for neutropenia during chemotherapy, and a lower threshold to administer S. marcescens targeted antibiotics may be warranted in these patients.

Effect of Fungicide Benoram Seed Treatment on Germination, Growth, and Yield in Summer-Type Soybean (종자소독제 Benoram 처리가 하대두의 발아, 후기생육 및 수량에 미치는 영향)

  • 정길웅;주정일
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.38 no.2
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    • pp.166-173
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    • 1993
  • Seed infection to pod and stem blight (Diaporthe phaseolorum) is severe at summer type soybeans maturing at hot and humid conditions. In order to increase germination and plant stand, this experiments were evaluated the effects of fungicide Benoram(Benomyl 20% ${\pm}$ Thiram 20%) seed treatments with different seed sizes on emergence yield at field conditions, and with different infection degrees to pod and stem blight on germination in laboratory. Benoram treatment on seed was improved emergence rate and elongated hypocotyl not increased growth and yield. It was clearly improved length but germinations and germination speed by Benoram fungicide treatment on severely infected seed however was not improved germination rate at good quality of seeds and the effect was higher at large seed cultivar than small ones.

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Isolate Virulence and Cultivar Response in the Winter Wheat: Pyrenophora tritici-repentis (Tan Spot) Pathosystem in Oklahoma

  • Kader, Kazi A.;Hunger, Robert M.;Payton, Mark E.
    • The Plant Pathology Journal
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    • v.37 no.4
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    • pp.339-346
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    • 2021
  • Prevalence of tan spot of wheat caused by the fungus Pyrenophora tritici-repentis has become more prevalent in Oklahoma as no-till cultivation in wheat has increased. Hence, developing wheat varieties resistant to tan spot has been emphasized, and selecting pathogen isolates to screen for resistance to this disease is critical. Twelve isolates of P. tritici-repentis were used to inoculate 11 wheat cultivars in a greenhouse study in splitplot experiments. Virulence of isolates and cultivar resistance were measured in percent leaf area infection for all possible isolate x cultivar interactions. Isolates differed significantly (P < 0.01) in virulence on wheat cultivars, and cultivars differed significantly in disease reaction to isolates. Increased virulence of isolates detected increased variability in cultivar response (percent leaf area infection) (r = 0.56, P < 0.05) while increased susceptibility in cultivars detected increased variance in virulence of the isolates (r = 0.76, P < 0.01). A significant isolate × cultivar interaction indicated specificity between isolates and cultivars, however, cluster analysis indicated low to moderate physiological specialization. Similarity in wheat cultivars in response to pathogen isolates also was determined by cluster analysis. The use of diverse isolates of the fungus would facilitate evaluation of resistance in wheat cultivars to tan spot.

Comparison of Bacterial Community of Healthy and Erwinia amylovora Infected Apples

  • Kim, Su-Hyeon;Cho, Gyoengjun;Lee, Su In;Kim, Da-Ran;Kwak, Youn-Sig
    • The Plant Pathology Journal
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    • v.37 no.4
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    • pp.396-403
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    • 2021
  • Fire blight disease, caused by Erwinia amylovora, could damage rosaceous plants such as apples, pears, and raspberries. In this study, we designed to understand how E. amylovora affected other bacterial communities on apple rhizosphere; twig and fruit endosphere; and leaf, and fruit episphere. Limited studies on the understanding of the microbial community of apples and changes the community structure by occurrence of the fire blight disease were conducted. As result of these experiments, the infected trees had low species richness and operational taxonomic unit diversity when compared to healthy trees. Rhizospheric bacterial communities were stable regardless of infection. But the communities in endosphere and episphere were significanlty affected by E. amylovora infection. We also found that several metabolic pathways differ significantly between infected and healthy trees. In particular, we observed differences in sugar metabolites. The finding provides that sucrose metabolites are important for colonization of E. amylovora in host tissue. Our results provide fundamental information on the microbial community structures between E. amylovora infected and uninfected trees, which will contribute to developing novel control strategies for the fire blight disease.

Control of oomycete pathogens during Pyropia farming and processing using calcium propionate

  • Yong Tae Kim;Ro-won Kim;Eunyoung Shim;Hana Park;Tatyana A. Klochkova;Gwang Hoon Kim
    • ALGAE
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    • v.38 no.1
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    • pp.71-80
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    • 2023
  • The oomycete pathogens Pythium porphyrae, causing red rot disease, and Olpidiopsis spp. causing Olpidiopsis-blight, cause serious economic losses to Pyropia sea farms in Korea. During the washing step for Pyropia processing, these pathogens proliferate rapidly, significantly reducing the quality of the final product. To develop non-acidic treatments for these pathogens, various calcium salts were tested against the infectivity of P. porphyrae and Olpidiopsis pyropiae on Pyropia gametophytes, and calcium propionate was the most effective. When Pyropia blades were immersed in 10 mM calcium propionate for 1 h after inoculation with the oomycete pathogen, infection rate of both oomycete pathogens on day 2 was significantly lower (7.1%) than control (>95%). Brief incubation of Pyropia blades in calcium propionate also reduced the spread of infection. The infected area of Pyropia thallus was reduced to 14.3% of the control in 2 days after treatment with 100 mM calcium propionate for 30 s. In field experiments conducted in actual aquaculture farms, it has been shown that a brief 30 s wash every two weeks with 100 mM calcium propionate can effectively reduce the spread of oomycetes throughout the entire culture period. The above results suggest that calcium propionate can be a useful means for controlling the spread of oomycetes not only during laver processing but also during aquaculture.

SARS-CoV-2 Infection of Airway Epithelial Cells

  • Gwanghui Ryu;Hyun-Woo Shin
    • IMMUNE NETWORK
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    • v.21 no.1
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    • pp.3.1-3.16
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    • 2021
  • Coronavirus disease 2019 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been spreading worldwide since its outbreak in December 2019, and World Health Organization declared it as a pandemic on March 11, 2020. SARS-CoV-2 is highly contagious and is transmitted through airway epithelial cells as the first gateway. SARS-CoV-2 is detected by nasopharyngeal or oropharyngeal swab samples, and the viral load is significantly high in the upper respiratory tract. The host cellular receptors in airway epithelial cells, including angiotensin-converting enzyme 2 and transmembrane serine protease 2, have been identified by single-cell RNA sequencing or immunostaining. The expression levels of these molecules vary by type, function, and location of airway epithelial cells, such as ciliated cells, secretory cells, olfactory epithelial cells, and alveolar epithelial cells, as well as differ from host to host depending on age, sex, or comorbid diseases. Infected airway epithelial cells by SARS-CoV-2 in ex vivo experiments produce chemokines and cytokines to recruit inflammatory cells to target organs. Same as other viral infections, IFN signaling is a critical pathway for host defense. Various studies are underway to confirm the pathophysiological mechanisms of SARS-CoV-2 infection. Herein, we review cellular entry, host-viral interactions, immune responses to SARS-CoV-2 in airway epithelial cells. We also discuss therapeutic options related to epithelial immune reactions to SARS-CoV-2.