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Immunoactivity of Ginsenosides Re and Rg1 that Enhances Resistance of Mice Against Experimental Disseminated Candidiasis  

Han, Yong-Moon (Department of Pharmacy, College of Pharmacy, Dongduk Women's University)
Jin, Byung-Suk (Department of Applied Chemistry, College of Natural Sciences, Dongduk Women's University)
Ko, Sung-Kwon (Korea Ginseng Institute, Chung-Ang University)
Lee, Jue-Hee (Department of Pharmacy, College of Pharmacy, Dongduk Women's University)
Publication Information
Natural Product Sciences / v.10, no.3, 2004 , pp. 134-139 More about this Journal
Abstract
In this study, an immunoactivity of panaxtriol ginsenosides Re and Rg1 against infection due to Candida albicans was investigated. The ginsenosides were extracted from Red Ginseng with 85% ethanol and heat-treatment and were analyzed by HPLC on water-acetonitrile as a mobile phase. The HPLC analysis revealed that the extract contained ginsenosides Re and Rg1, which were eluted as a combined peak. By agar diffusion susceptibility, the mixture of Re and Rg1 had no growth-inhibitory activity on C. albicans yeast cells. However, in animal tests BALB/c mice given the mixture of Re and Rg1 intraperitoneally (Lp.) before intravenous (Lv.) infection with live C. albicans yeast cells had longer mean survival times (MST) than MST of control mice groups that received only buffer solution instead of Re and Rg1. In experiments 60% of the ginsenosides-treated mice survived the entire duration of the 50-day observation. The Re and Rg1 mixture induced production of nitric oxide when interacted with RAW 264.7 macrophage cell line. In addition, the mixture caused morphological change of the macrophages. These data indicate that immunostimulation by the Re and Rg1 may be responsible for the protection of mice against disseminated candidiasis.
Keywords
Red Ginseng; Re and Rg1; immunoactivity; Candida albicans; protection;
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1 Bondar, O. P., Saad L. M., Shaturskii, laP, and Kholodova, lud Comparative characteristics of color reaction to ecdysteroids. Ukr. Biokhim, Zh. 65, 83-87 (1993)
2 Chen, X. and Lee, T.J.F. Ginsenosides-induced nitric oxide-mediated relaxation of the rabbit corpus cavemosum. Br. J. Pharmacol. 115, 15-18 (1995)   DOI   PUBMED   ScienceOn
3 Gillis, N. C., Panax ginseng pharmacology: A nitric oxide link? Biochem Pharmacol. 54, 1-8 (1997)   DOI   PUBMED   ScienceOn
4 Han, Y. Grape seed extract protects mice aginats disseminated candidiasis. Nat. Pro. Sci. 9, 60-63 (2003-b)
5 Han, B. H., Han, Y. N., and Park, M. H. Chemical and bioche-mical studies on antioxidant components of ginseng, In: Advaces in chinese medicinal materials research. Eds. Chang, H. M., Yeung, H. W., Tso, W. W., and Koo, A. World Scientific Press, Singapore, pp. 485-498 (1985)
6 Han, Y. and Cutler, J. E. Antibody response that protects against disseminated candidasis. Infect. Immun. 63, 2714-2719 (1995)   PUBMED
7 Han, Y., Granger, B., and Cutler, J. E. Immunoprotection against candidiasis. In: Candida and candidosis. ASM Press, Washing-ton, D. C. pp. 243-256 (2003)
8 Han, Y., Riesselman, M. H., and Cutler, J. E. Protection against candidiasis by an immunoglobulin G3 (IgG3) monoclonal antibody specific for the same mannotriose as an IgM protective antibody. Infect. Immun. 68, 1649-1654 (2000)   DOI   ScienceOn
9 Huang, K. C. The pharmacology of Chinese herbs. CRC Press. Boca Raton. FL. (1999)
10 Tang, W. and Eisenbrand, G. Panax ginseng C. A. Meyer. In: Chinese drugs of plant origin, Springer, London. pp. 711-737 (1992)
11 Benishin, C. G. Actions of ginsenoside Rb1 on choline uptake in central cholinergic nerve endings. Neurochem. Int. 21, 1-5 (1992)   DOI   PUBMED   ScienceOn
12 Han, Y. A role of neutrophil in anti-Candida monoclonal antibody protection against vaginal infection due to Candida albicans. Yakhak Hoeji, 47, 190-194 (2003-a)
13 Yamaguchi, Y., Haruta, K., and Kobayashi, H. Effects of ginsenosides on impaired on performance induced in the rat by scopolamine in a radial-arm maze. Psychoneuroendocrinol. 20, 645-653 (1995)   DOI   ScienceOn
14 Sonnenborn, U. and Proppert, Y. Ginseng (Panax ginseng C. A Meyer). Br. J. Phytother. 2, 3-14 (1991)
15 Han, Y., Ulrich, M. A., and Cutler, J. E. Candida albicans mannan extract-protein conjugates induce a protective immune response against experimental candidiasis. J. Infect. Dis. 179, 1477-84 (1999)   DOI   PUBMED   ScienceOn
16 Benishin, C. G., Lee, R., Wang, L. C. H., and Liu, H. J. Effects of ginsenoside Rb1 on central cholinergic metabolism. Pharmacol. 42, 223-229 (1991)   DOI   ScienceOn
17 Body, G. P. The emergence of fungi as major hospital pathogens. J. Hosp. Infect. 11(Suppl A), 411-26 (1988)   DOI
18 Shibata, S. Chemistry and cancer preventing activities of ginseng saponins and some related triterpenoid compounds. J. Korean Med. Sci. 16(Suppl), S28-37 (2001)
19 Edwards, J. E. 1991. Invasive Candida infections: evolution of a fungal pathogen. N. Engl. J. Med. 324, 1060-2 (1991)   DOI   PUBMED   ScienceOn
20 Han, Y., Kozel, T. R., Zhang, M. X, MacGill, R. S., Carroll, M.C. and Cutler, J. E. Complement is essential for protection by an IgM and an IgG3 monoclonal antibody against experi-mental, hematogenously disseminated candidiasis. J. Immun. 167, 1550-1557 (2001)
21 Attele, A. S., Wu, J. I., and Yuan, C. Ginseng pharmacology: Multiple constituents and multiple actions. Biochem. Pharmacol. 58, 1685-1693 (1999)   DOI   PUBMED   ScienceOn
22 Kang, S. Y., Schini-Kerth, V. B., and Kim, N. D. Ginsenosides of the protopanaxtriol group cause endothelium-dependent relaxation in the rat aorta. Life Sci. 56, 1577-1586 (1995)   DOI   ScienceOn
23 Tsang, D., Yeung H. W., Tso, W. W., and Peck, H. Ginseng saponins: Influence on neurotransmitter uptake in rat brain synaptosomes. Planta. Med. 3, 221-224 (1985)   PUBMED
24 Lee, F. C. Facts about ginseng, the elixir of life. Hollyn International Corp., Elizabeth, NJ. (1982)