• Applied Chemistry for Engineering
• /
• v.10 no.2
• /
• pp.263-267
• /
• 1999

NO removal activity (per unit of mass) of the used catalyst was seriously decreased as low as 27% of the new catalyst. Since the surface area of the used catalyst was 63% of that of the new one, the mojor reason for the lessened activity of the used catalyst compared to the new one may be due to the decreased surface area by sintering and surface concentration of active materials. Poison may be regarded as another important factor, since it affect the active site of catalyst by heavy metals. To recycle the used catalyst, we focused on the removal of poisoning agents from the catalyst. By using $80^{\circ}C$ water for 30 min upto 2 h, the recycled catalyst demonstrated the best activity and efficiency, which may be due to the removal of both K and Na. Although the recovered activity (per unit of surface area) of the catalyst was 79% compared to the new one, the activity (per unit of mass) of the recovered catalyst was only 49% compared of the activity of fresh catalyst.

• Journal of Drive and Control
• /
• v.17 no.3
• /
• pp.26-32
• /
• 2020

In modern society, the energy-saving problem of industrial vehicles is economically and environmentally critical. Energy savings using the potential energy of forklifts are one of the viable solutions to resolving this problem. The basic concept of this study is to operate the hydraulic motor and recharge the battery using the flow rate from the cylinder when loading heavy objects and lowering the fork. To save energy, the torque and rotational speed of the generator should be optimized according to the load and descent speed to increase efficiency. To this end, we propose a system that optimizes energy saving efficiency by controlling the swashplate angle of the variable hydraulic motor through the GA(Genetic-Algorithm). The results were verified by building and comparing fixed motor models and variable motor models using the AMEsim. The results of the study show that the proposed optimized swashplate angle increases the energy saving efficiency by approximately 6%-8%, depending on the working conditions.

• Journal of Digital Convergence
• /
• v.14 no.7
• /
• pp.397-402
• /
• 2016

This study discusses on the city-culture contents convergence design strategy which can be linked to the identity of the city through applying the graffiti on old-town of Dongducheon. First, the background and cultural asset of Dongducheon were researched. Second, the places revealing the identities were investigated. Third, the interviews with the college students in the city were performed. As the result, we found that using graffiti gains positive reaction as the reinforcement factor for the city identification. The exotic image made up by US military presence is evaluated as an important factor for the city identification. The Rock Festival is a representative cultural event derived by the cultural influence from the US military and is one of the crucial elements for the city identification. In this study, graffiti was proposed as the visual expression which can be harmonized with this culture and be reinforced for the city identity. Results of the study concludes with the strategy for the application of graffiti with the expectation for the improvement of city image of Dongducheon through the change of identity, urban regeneration, and making of pollution-free culture.

• Journal of Microbiology and Biotechnology
• /
• v.29 no.4
• /
• pp.577-586
• /
• 2019

The engineered Aspergillus oryzae has a high NADPH demand for xylose utilization and overproduction of target metabolites. Glucose-6-phosphate dehydrogenase (G6PDH, E.C. 1.1.1.49) is one of two key enzymes in the oxidative part of the pentose phosphate pathway, and is also the main enzyme involved in NADPH regeneration. The open reading frame and cDNA of the putative A. oryzae G6PDH (AoG6PDH) were obtained, followed by heterogeneous expression in Escherichia coli and purification as a his6-tagged protein. The purified protein was characterized to be in possession of G6PDH activity with a molecular mass of 118.0 kDa. The enzyme displayed maximal activity at pH 7.5 and the optimal temperature was $50^{\circ}C$. This enzyme also had a half-life of 33.3 min at $40^{\circ}C$. Kinetics assay showed that AoG6PDH was strictly dependent on $NADP^+$ ($K_m=6.3{\mu}M$, $k_{cat}=1000.0s^{-1}$, $k_{cat}/K_m=158.7s^{-1}{\cdot}{\mu}M^{-1}$) as cofactor. The $K_m$ and $k_{cat}/K_m$ values of glucose-6-phosphate were $109.7s^{-1}{\cdot}{\mu}M^{-1}$ and $9.1s^{-1}{\cdot}{\mu}M^{-1}$ respectively. Initial velocity and product inhibition analyses indicated the catalytic reaction followed a two-substrate, steady-state, ordered BiBi mechanism, where $NADP^+$ was the first substrate bound to the enzyme and NADPH was the second product released from the catalytic complex. The established kinetic model could be applied in further regulation of the pentose phosphate pathway and NADPH regeneration of A. oryzae to improve its xylose utilization and yields of valued metabolites.

• 한국생물공학회:학술대회논문집
• /
• 2000.04a
• /
• pp.215-218
• /
• 2000

Aspergillus oryzae is a filamentous fungus classified in the group Aspergillaceae Ascomycetes. It is an important microorganism for industrial production of enzymes and fermented food productions. The genetic transformation system in A. oryzae was used to protoplast mediated transformation with PEG/$CaCl_2$. When the protoplast was used, the regeneration efficiency was decreased and then transformation frequence was also effected. In this study, fungal transformation was carried out by bypassing the protoplast isolation step, changing enzymes, such as hemicellulase and celluclast, and decreasing the culturing time for the increment of the transformation efficiency. 83 transformants/10ug of DNA with hemicellulase were obtained, compared with less than 10 transformants with novozyme234 and celluclast.

• Proceedings of the KIPE Conference
• /
• 1997.07a
• /
• pp.14-20
• /
• 1997

Due to several advantages of Pulse Width Modulated(PWM) Converter, such as unity power factor with low-harmonics and energy regeneration, PWM converter has been widely used in industrial application. In every application of energy conversion equipment, the design and implementation must be carried out considering performance and cost. High quality with low cost is the best choice for energy conversion equipment. High dc link voltage can reduce inverter and motor side losses and system dimension compare to low dc link voltage. Analog controller can make PWM converter cheaper without considerable degradation of the performance than digital controller. This paper shows the simplified analog controller-for 600V dc link voltage using stationary reference frame control and the simulation results.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.17 no.1
• /
• pp.109-113
• /
• 2008

Realizing the scope of utilizing by-products of silk cocoons by applying appropriate methods is the immediate crave to optimize returns. The nutritive value of pupae suits for human diet, feed for poultry, carps, fish, rabbits, piggery and dogs. The pupal skin, fat, oil, cocoon palade have applications in oleo chemical, soap, glycerin, cosmetic, artificial fibres, membranes and n-triacontanol isolation. The pupal proteins Chitin, Shinki fibroin, Serrapeptidase, glucosamine are latent precursors of post surgical, anti-carcinogenic, anti-inflammative, anti-bacterial, anti-histaminic, gastric, hepatitis, pancreatitis, leukocytopenia, neurological, ophthalmic, blood pressure, cardiac and diabetic medicines and for preparation of vitamins A, E and K. The silk and its proteins sericin and fibroin are potentially used for wound healing, diabetes, impotence, sinusitis, arthritis, edema, cystitis, epididymitis, tissue regeneration, cancer, post-surgical trauma and used as anti-oxidatives, bio-adhesives, ultra violet screens and bio-active textiles. The waste cocoons can be used in making art crafts like garlands, carpets, overcoats, decoratives and greeting cards. The in-depth research towards utility optimization and make aware this reality to sericulturists, reelers, weavers, traders, entrepreneurs, policy makers etc., is the upright want of the today's Sericulture industry.

• Proceedings of the KAIS Fall Conference
• /
• 2011.12b
• /
• pp.453-456
• /
• 2011

전 세계적으로 도시수변공간 개발이 이슈화 되고 있는 요즘. 우리나라에서도 급속한 경제 발전에 따라 회손 되어버린 하천들의 정비사업이 활성화 되고 있다. 이에 본 연구에서는 도시수변공간과 도시재생에 대해 조사하여 이해하고 그 사례를 들어 어떠한 방법들로 개발되었는지 살펴본 뒤, 천안천의 형장조사를 통해 상류, 중류, 하류로 나누어 각각의 구역이 어떠한 방식으로 개발되었으며 현재 이용자들에게 어떻게 활용되는지를 파악하였다.

• Proceeding of Spring/Autumn Annual Conference of KHA
• /
• 2008.11a
• /
• pp.442-447
• /
• 2008

With the advances in the high-tech knowledge and service industries, along with a high-flying economy, the industrial structures of many cities have changed. The subject of the successful cases of dimensional integrated complexes in Roppongi Hills, the actions and preferences of these complexes'users were surveyed and analyzed in this study to determine the factors that contribute to the success or failure of such development projects. The results of the survey that was conducted in this study regarding the satisfaction of the residents of Roppongi Hills with such complexes will be considered in the formulation of a construction plan and will be used as base data for designing a large-scale dimensional integrated complex in Korea.

• Proceedings of the KAIS Fall Conference
• /
• 2005.05a
• /
• pp.269-271
• /
• 2005

본 연구는 수선화 구근의 각 조직으로부터 기내배양시 캘러스형성과 신초재분화를 위한 성장조절제의 효과를 측정하기 써하여 시행되었다. 신초형성과 callus의 형성은 기저부를 포함하는 floral axis에서 $50\%$의 신초발아율을 관찰하였고 scale 에서는 신초형성이 거의 관찰되지 않았다. 그리고 floral axis만을 치상한 경우는 아주 저조한 신초형성율이 관찰되었다. 지저부를 포함한 floral axis의 신초형성은 callus의 형성과 같은 NAA 0.5 mg/L과 BA 1.0 mg/L을 포함하는 MS 배지에서 만족할만한 결과를 얻었다. 신초형성으로부터 모든 신초가 형성되기까지는 약 140일이 소요되었다. 신초가 형성된 구근조직을 NAA 5.0 mg/L과 TDZ 0.02 mg/L을 포함하는 뿌리유도 MS배지에서 뿌리가 유도되는 결과를 얻었다. 본 실험에서는 수선화의 재 분화에 있어서 구근의 조직에 따라 신초형성이 다르게 나타남을 발견 할 수 있었다.