• Title/Summary/Keyword: industrial biotechnology

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Bioactive Compounds, Anti-Oxidant Activities and Anti-Inflammatory Activities of Solvent Extracts from Centella asiatica Cultured in Chungju (충주산 병풀 용매추출물의 활성성분과 항산화 및 항염증 활성)

  • Shin, Hyun-Young;Kim, Hoon;Jeong, Eun-Jin;Kim, Jeung-Eun;Lee, Kyeong-Haeng;Bae, Yun-Jeong;Yu, Kwang-Won
    • The Korean Journal of Food And Nutrition
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    • v.33 no.6
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    • pp.692-701
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    • 2020
  • Centella asiatica (CA) has been widely used as herbal plants. It is a valuable resources. The aim of the present study was to evaluate physiological activities of solvent extracts from CA cultivated in Chungju, Korea (Good tiger care). After preparing water (cold-water, CA-WE; hot-water, CA-HWE) and EtOH extracts (50% EtOH, CA-50E; 70% EtOH, CA-70E), their total polyphenol, flavonoid, and triterpenoid contents, and anti-oxidant activities, and anti-inflammatory activities were examined and compared. CA-70E showed the most potent anti-oxidant activity based on ABTS radical scavenging activity and reducing ability assays, while CA-50E and CA-70E showed the highest DPPH radical scavenging activity. Among major triterpenoid glycosides present in CA, madecassoside and asiaticoside contents were found to be the highest in CA-70E, and madecassic acid and asiatic acid were the highest in CA-50E. In LPS-stimulated RAW 264.7 cells, CA-70E showed the highest TNF-α inhibitory activity, although CA-50E and CA-70E similarly inhibited nitric oxide production. Ethanol extracts significantly inhibited IL-6 and IL-8 production more than water extracts using TNF-α/IFN-γ-stimulated HaCaT cells, indicating its better inhibitory against skin inflammation. Therefore, Chungju-CA EtOH extract, especially 70% EtOH extract, has high physiologically active ingredients and potent anti-oxidant and anti-inflammatory activities, suggesting its industrial application as a functional material.

A Flora of Vascular Plants of Seondalsan Mountain (Bonghwa-gun, Gyeongsangbuk-do) (선달산(경상북도 봉화군) 일대의 관속식물상)

  • AN, Min Woo;Jeong, Seon;Kim, Jae Young;Jo, Hyeong Jun;Heo, Tae Im;Lee, Hye Jeong;Seo, Eul Won;Chung, Gyu Young
    • Korean Journal of Plant Resources
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    • v.35 no.2
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    • pp.289-316
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    • 2022
  • This study was investigated to elucidate the distribution of vascular plants and their usefulness of Seondalsan Mountain (1,236 m) in Bonghwa-gun, Gyeongsangbuk-do. The surveys were carried out 20 times from April in 2014 to October in 2020. The vascular plants of Seondalsan Mountain was consisted a total of 638 taxa based on the voucher specimens; 96 families, 341 genera, 562 species, 18 subspecies, 53 varieties, 5 forms. 20 taxa of the Korean endemic plants were recorded and 1 taxa of Critically Endangered (CR), 3 taxa of Vulnerable (VU) and 9 taxa of Least Concern (LC), designated by Korean Forest Service as rare plants, were investigated in this region. Furthermore, V, IV and III degrees of floristic regional indicator plants, designated by the National Institute of Ecology, were included 1 taxa, 11 taxa and 28 taxa, respectively. Among them, edible, medicinal, fragrant, industrial, ornamental, ecological restoration, forage/compost plants were included 440 taxa, 505 taxa, 19 taxa, 234 taxa, 335 taxa, 214 taxa and 269 taxa respectively. In addition, 30 taxa of naturalized plants were observed.

Transparent Near-infrared Absorbing Dyes and Applications (투명 근적외선 흡수 염료 및 응용 분야)

  • Hyocheol Jung;Ji-Eun Jeong;Sang-Ho Lee;Jin Chul Kim;Young Il Park
    • Applied Chemistry for Engineering
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    • v.34 no.3
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    • pp.207-212
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    • 2023
  • Near-infrared (NIR) absorbing dyes have been applied to various applications such as optical filters, biotechnology, energy storage and conversion, coating additive, and traditionally information-storage materials. Because image sensors used in cellphones and digital cameras have sensitivity in the NIR region, the NIR cut-off filter is essential to achieving more clear images. As energy storage and conversion have been important, diverse NIR absorbing materials have been developed to extend the absorption region to the NIR region, and NIR absorbing materials-based research has proceeded to improve device performances. Adding NIR-absorbing dye with a photo-thermal effect to a self-healable coating system has been attractive for future mobility technology, and more effective self-healing properties have been reported. In this report, the chemical structures of representative NIR-absorbing dyes and state of the art research based on NIR-absorbing dyes are introduced.

Importance-Performance Analysis(IPA) of the selection attributes of functional cosmetics (기능성화장품 선택속성의 IPA(중요도-만족도) 분석)

  • Han, Do-Kyung;Lee, Hyun-Jun;Paik, Hyun-Dong;Shin, Dong-Kyoo;Park, Dae-Sub;Hwang, Hye-Sun;Hong, Wan-Soo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.6
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    • pp.527-536
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    • 2016
  • This study aims to generate baseline data for vitalizing the sales of functional cosmetics through an Importance-Performance Analysis (IPA) of the selection attributes of functional cosmetics. From the analysis of consumers' selection criteria, the study will assist functional cosmetics companies in reflecting consumer demands and therefore securing competitiveness. For this, general consumers aged over 20 years were surveyed for 5 weeks from Feb 23 through Mar 30, 2015, and 447 empirical data (response rate 88.9%) were processed through SPSS WIN 21.0 program for analysis. To conduct gender difference analysis on the IPA of the selection attributes of functional cosmetics, 17 selection attributes were categorized into 4 factors: functionality, labeling, popularity, and product. Cronbach's alpha for all factors was 0.5, proving the internal consistency and reliability of the survey. The survey results showed that while the entire average came out significantly higher for females (5.89/7points) than for males (5.66/7points) (p<0.001), the selection attributes 'anti-wrinkling', 'whitening function', 'functionality', 'expiration date', 'full ingredient labeling system' and 'various promotional events' showed significant gender differences. IPA results pertaining to gender showed 'price', 'functionality', 'spreadability' and 'full ingredient labeling system' as 2nd quadrant attributes, whereas female consumers selected 'price', 'whitening function', 'anti-wrinkling', 'functionality' and 'full ingredient labeling system' as attributes. Results show that businesses in the field of cosmetics and related areas need to prioritize improving the following factors that received low satisfaction from all consumers: 'price', 'functionality', and 'total labeling.' In particular, the 'price' aspects are considered to require reasonable and affordable pricing.

Physicochemical Characteristics and Volatile Compounds Analysis of Coffee Brews according to Coffee Bean Grinding Grade (커피원두의 분쇄입도에 따른 커피 추출물의 이화학적 품질특성 및 휘발성 향기성분 분석)

  • Lim, Heung-Bin;Jang, Keum-Il;Kim, Dong-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.46 no.6
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    • pp.730-738
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    • 2017
  • In this study, we investigated the physicochemical properties of coffee brews according to coffee bean grinding grade. We also examined the effect of grinding grade on amounts of volatile flavor compounds. Coffee brew samples were separated using standard sieves (with pore sizes of 850, 600, and $425{\mu}m$), making particle sizes of ground beans as follows: whole bean (control), $850{\mu}m$ or more (coarse), $850{\sim}600{\mu}m$ (medium), $600{\sim}425{\mu}m$ (fine), and $425{\mu}m$ or less (very fine). For each particle size category, pH, total acidity, brown color intensity, chromaticity, total phenolic content, caffeine content, chlorogenic acid content, and total amounts of volatile flavor compounds generated were compared and analyzed. As grinding grade decreased, pH and brown color intensity increased from 4.84 to 5.18 and from 0.257 to 0.284, respectively, whereas total acidity decreased from 0.31 to 0.17%. As grinding grade decreased, the $L^*$ and $a^*$ color values decreased; however, $L^*$ value did not exhibit a significant difference depending on the grinding grade. The $b^*$ value was 15.75 in the very fine size category, which showed the highest yellowness. There was an 11 or higher color difference between the control and ground coffee powder, indicating a remarkable color difference. The total phenolic, caffeine, and chlorogenic acid contents of the coffee brewed from ground beans with a very fine size were 4.54 mg gallic acid equivalent/mL, $733.0{\mu}g/mL$, and $383.7{\mu}g/mL$, respectively, which were high values. The total amounts of volatile compounds in the very fine size category were found to be greater than 100 mg/kg. In this study, we suggest the basis for coffee quality evaluation, which involves evaluating changes in the physicochemical properties and amounts of flavor compounds of coffee relative to the grinding grade of the beans (basic step of coffee extraction).

A Study on the Analysis of Shipment Structure of Hallabong Tangor in Korea (한라봉의 출하구조 분석)

  • Ko, Seong-Bo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.7
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    • pp.670-676
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    • 2017
  • The purpose of this study is to analyze the shipbuilding structure of Hallabong Tangorover ten-day and monthly intervals, to determine whether the shipment control can stabilize and increase the income of Hallabong Tangor farmers by estimating and analyzing the price flexibility function of Hallabong, and to derive the policy implication. Looking at the overall market, the 1% increase in shipment volume indicates that the coefficient of price flexibility varies with time. The largest decline is in late December and early January. The dummy variable indicating the year in which the quality drops somewhat is statistically significant from March to May, and the average of them is -0.337%. This implies a greater importance for quality control and shipment control when quality is worse than good quality. The price flexibility, which indicates price changes due to 1% increase in monthly shipment volume of Hallabong Tangor, varies with time. The coefficient of price flexibility was the highest at -0.46 in December, but prices fell slightly due to quality improvement and the increase in special demand of New Year's demand, which was -0.33% in January, -0.261% in February, -0.307% in March, and -0.318% in April. Since then, the amount of storage has been gradually exhausted, and the price flexibility has fallen slightly to -0.243% in May and -0.236% in June. DUM02, which represents the year when more than 7 days after the New Year holidays are in February is from February to June (+). This indicates that the price decrease due to increase in volume is less than that in January when the New Year is in February. This indicates a greater necessity for shipment control when New Year holidays are in January.

Transcriptomic Profile Analysis of Jeju Buckwheat using RNA-Seq Data (NA-Seq를 이용한 제주산 메밀의 발아초기 전사체 프로파일 분석)

  • Han, Song-I;Chung, Sung Jin;Oh, Dae-Ju;Jung, Yong-Hwan;Kim, Chan-Shick;Kim, Jae-hoon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.1
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    • pp.537-545
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    • 2018
  • In this study, transcriptome analysis was conducted to collect various information from Fagopyrum esculentum and Fagopyrum tataricum during the early germination stage. Total RNA was extracted from the seeds and at 12, 24, and 36 hrs after germination of Jeju native Fagopyrum esculentum and Fagopyrum tataricum and sequenced using the Illumina Hiseq 2000 platform. Raw data analysis was conducted using the Dynamic Trim and Lengths ORT programs in the SolexaQA package, and assembly and annotation were performed. Based on RNA-seq raw data, we obtained 16.5 Gb and 16.2 Gb of transcriptome data corresponding to about 84.2% and 81.5% of raw data, respectively. De novo assembly and annotation revealed 43,494 representative transcripts corresponding to 47.5Mb. Among them, 23,165 sequences were shown to have similar sequences with annotation DB. Moreover, Gene Ontology (GO) analysis of buckwheat representative transcripts confirmed that the gene is involved in metabolic processes (49.49%) of biological processes, as well as cell function (46.12%) in metabolic process, and catalytic activity (80.43%) in molecular function In the case of gibberellin receptor GID1C, which is related to germination of seeds, the expression levels increased with time after germination in both F. esculentum and F. tataricum. The expression levels of gibberellin 20-oxidase 1 were increased within 12 hrs of gemination in F. esculentum but continuously until 36 hrs in F. tataricum. This buckwheat transcriptome profile analysis of the early germination stage will help to identify the mechanism causing functional and morphological differences between species.

Heavy Metal and Amino Acid Contents of Soybean by Application of Sewage and Industrial Sludge (생활하수 및 산업폐수 슬러지 처리에 따른 콩의 중금속 및 아미노산 함량)

  • Moon, Kwang-Hyun;Kim, Jae-Young;Chang, Moon-Ik;Kim, Un-Sung;Kim, Seong-Jo;Baek, Seung-Hwa
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.2
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    • pp.268-277
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    • 2013
  • This study investigates the effects of accumulated levels of heavy metals and nutrients of cultivated soybean plant tissues, after the continuous application of sewage sludge (SS) and industrial sludge (IS). SS and IS were applied to soybean plants at loading of 0, 11.25, 22.50, and 45.00 Mg/ha, and the contents of heavy metals (Cd, Pb, Ni, Cu, and Zn), proteins, and amino acids in the cultivated soybean plants were measured. The Cd content in the soybean was 0.02~0.05 mg/kg, which is within the safety level set in the standard, and that of Pb was 0.02~0.15 mg/kg, which is also within the safety level except for IS 45 Mg/ha. The soybean harvest quantity was higher in the treatment groups than the control group in the first year. However, in the second year, SS had lower harvest and IS had the same level or a decreasing tendency, compared with the control group. In the first year, the content of amino acid which followed handling of SS was increased in the sludge groups more than in the control group in the case of glutamate. However, the influence of continuous application was increased in the sludge groups in the case of amino acids of 12 types. In conclusions, the accumulation in soybean of heavy metals by sludge treatment is not a problem, but the decreased yields needs to be considered. In addition, the most appropriate level of sludge treatment was 11.25 Mg/ha.

A Study on Researches of Resource-plants for Special Use or Purpose - Based on the Articles Published in the Journal of Korean Forestry - (특용자원식물(特用資源植物)의 연구(硏究) - 한국임학회지에 게재된 논문을 중심으로 -)

  • Yi, Jae-Seon;Kim, Chul-Woo;Song, Jae-Mo;Bae, Chan-Ho;Kang, Hyo-Jin;Hwang, Suk-In;Moon, Heung-Kyu
    • Journal of Forest and Environmental Science
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    • v.19 no.1
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    • pp.85-98
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    • 2003
  • The articles, published in the Journal of Korean Forestry from Number 1(1962) to Number 6, Volume 91(2002), were surveyed and investigated for the research trend analysis about resource-plants for special use or purpose, i. e., edible plants, medicinal plants, feed resource, landscape plants, fiber plants, industrial usuage, and bee plants. If the purpose or subject matter of the research was construction or furniture timber production, mushrooms and/or pulp and paper, such research was not included in this study. These articles were classified again depending on the content of research into 14 categories: habitat environment, ecology, physiology, propagation, silviculture (tending and culture), genetics and breeding, identification, insect and disease control, animal-related research, component analysis, vegetation survey, biotechnology, management, and review. Among the total 1.434 articles published, 396 ones (27.6%) were related with plants for special use or purpose. Vegetation survey was 60 (15.2%): physiology 56(14.1%) : genetics and breeding 56(14.1%): propagation 53(13.4%): and ecology 37(9.3%). Siviculture research field included 11 articles (2.8%), which indicates that the management of resource-plants is so far from economic income as seen in the low number of management research filed articles, i. e., only 6 reports (1.5%) Korean white pine was most popular for research and included 42 articles: Robinia pseudoacacia 23: Castanea crenata 14: and ginkgo tree 14. Research related with these species had focused mainly on propagation, physiology, genetics and breeding, ecology and pest control. Based on this survey and analysis, the followings are suggested: 1. More research is required on forest herbaceous plants. 2. Cooperative research work with other industrial and/or scientific area is recommendable for commercialization including medicine, cosmetics, and food etc. 3. Research on resource-plant conservation, which includes biology, social education and policy, should be supported for next generation. 4. Mutual correspondence and information exchange about the research results between researchers and institutes is more necessary than now.

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consumers' purchasing behavior of functional cosmetics and Inula based functional cosmetics merchandising research (국내 소비자의 기능성화장품 구매행태 및 선복화 활용 기능성화장품 상품화를 위한 연구)

  • Han, Do-Kyung;Lee, Hyun-Jun;Lee, Eun-Hee;Paik, Hyun-Dong;Shin, Dong-Kyoo;Park, Dae-Sub;Hwang, Hye-Seon;Hong, Wan-Soo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.8
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    • pp.236-250
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    • 2016
  • This study was conducted to provide baseline data regarding functional cosmetics so that Inula. based cosmetics can increase its competitiveness in the market as well as to understand current trends to enable anticipation of demands for future product development. For this research, general consumers over the age of 20 residing in Seoul and the Gyeonggi district were surveyed. The results show consumers preferred serum-type products among various types of cosmetics, and that they purchased these once every 1-3 months. Consumers also preferred these products in less than 10-30ml capacity, and at costs of less than 30,000-50,000 KRW. For whitening, functional cosmetics consumers also preferred the serum type, in less than 30-50ml capacity and priced less than 30,000-50,000 KRW. Consumers preferred to purchase functional cosmetics in single units. The major purchasing location, with a high preference rate, was cosmetic stores, and the major sources of information, also with high preference rates, were 'experienced reviews from family, friends and acquaintances' and 'TV advertisements'. Respondents selected 'over 50,000 KRW' the most for all items when responding to 'Purchase Intent for Functional Cosmetics containing Inula', and responded that they were willing to pay 10%-30% more for functional cosmetics containing Inula compared to standard functional cosmetics. These results show that businesses in the cosmetics industry need to take consumer demand into account when developing new functional cosmetic products, as well as establish plans to create specialized spaces that provide better quality service and increase word of mouth effect through better utilization of various types of offline media, social media, and blogs. The study also shows a need for businesses to develop products fully utilizing the Inula flower, which has been shown to be effective as a natural skin whitener, wrinkle reducer and skin moisturizer, to appeal to the increasing number of customers interested in health and beauty.