• 약학회지
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• 제43권2호
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• pp.198-207
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• 1999

The aim of the present investigation was to examine whether YH439, a hepatoprotective agent, exerts protective effect against hepatotoxicity and reduces the production of cytokines and NO in lipopolysaccharide (LPS)-primed rats with carbon tetrachloride ($CCl_4$). Administration of LPS following a single dose of CCl4 injection resulted in remarkable elevations of the serum $TNF{\alpha},{\;}IL-l{\beta$ and IL-6 level. The serum NO level was moderately elevated and severe liver damage was evidenced by increases in serum alanine aminotransferase (ALT) and sorbitol dehydrogenase (SDH) activities. YH439 decreased the levels of TNF, $IL-l{\beta}$, IL-6, ALT, SDH as well as NO in the serum elevated by CCl4+LPS in a dose-dependent manner. Inducible nitric oxide synthase (iNOS) level was decreased in the liver of rats treated with YH439. The increased iNOS activity induced by LPS and $interferon-{\gamma}$ was significantly decreased in RAW 264.7 cells by YH439 treatment. YH439 increased the GSH level decreased by $CCl_4+LPS$ and suppressed the ratio of GSSG/GSH. The reduction of hepatotoxicity by YH439 may associated with the decrease in the production of cytokines as well as suppression of iNOS protein in conjunction with an increase in the GSH level.

• 약학회지
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• 제43권2호
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• pp.214-220
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• 1999

Present study was aimed to examine whether indomethacin affected the production of NO in the rat paw exudate by carrageenin. Paw edema and nitrite/nitrate levels in the paw exudate were maximal after 4 h and remained elevated up to 10 h, whereas indomethacin (10 mg/kg, po) significantly inhibited the carrageenin-induced paw edema and levels of nitrate in the paw exudate. However, paw edema and nitrite/nitrite levels were increased thereafter for 10 h. Indomethacin also enhanced the expression of iNOS mRNA and protein 4 h after carrageenin infection. Indomethacin inhibited the level of $PGE_2$ in the paw exudate in a time-dependent manner. These results suggest the possibility that indomethacin may potentiate expression of iNOS and subsequently increase nitrite/nitrate level in the late phase of carrageenin-induced rat paw inflammation possibly by suppressing cycloxygenase activity.

• Journal of Acupuncture Research
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• 제22권2호
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• pp.111-121
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• 2005

Human placental extract (HPE), which is prepared from the placenta of healthy pregnant females, has been widely used in clinical field. HPE is known to possess anti-inflammatory, anti-viral, anti-oxidative, anti-mutagenic, and analgesic properties. In this study, the effect of HPE against lipopolysaccharide (LPS)-induced inflammation was investigated. From the present results, HPE was shown to suppress prostaglandin E2 synthesis (PGE2) and nitric oxide (NO) production by inhibition on the LPS-stimulated enhancement of the cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) expressions in mouse BV2 microglial cells. These results suggest that HPE may offer a valuable mean of therapy for the treatment of brain inflammatory diseases by attenuating LPS-induced PGE2 and NO production.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.313.1-313.1
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• 2002

Nitric oxide (NO) production through the inducible nitric-oxide synthase (iNOS) pathway has been implicated in inflammatory diseases and cellular injury. Inhibition of various genes related to inflammation, including iNOS is one of the major roles of well-known anti-inflammatory drugs. In the present study, the effects of ceramide analogs on iNOS expression and NO production were evaluated to investigate how ceramide and its structurally related analogs modulate NO-mecliated cellular signals and inflammation. (omitted)

• 한국식품과학회지
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• 제46권4호
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• pp.511-515
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• 2014

각종 염증성 질환 및 패혈증으로 인한 치명적인 저혈압을 예방치료하는 약물 개발을 위한 기초 연구로서 유도성 NOS (inducible nitric oxide synthase, iNOS) 에 의한 NO의 과다 생성을 저해하는 성분을 천연물로부터 찾아내고자 본 연구를 수행하였다. NO 생성 저해활성의 검정은 대식세포주인 RAW 264.7 세포를 LPS로 활성화한 후, 유도되는 iNOS에 의해 생성되는 NO를 Griess 시약을 이용해 $NO_2{^-}$의 형태로 정량하였다. 또한 Western blot 실험 및 RT-PCR 실험을 시행하여 iNOS의 mRNA의 발현 및 단백 합성에 대한 영향을 조사하였다. 고량강(Alpinia officinarum Hance, Zingiberaceae)의 메탄올 추출물로부터 극성에 따른 용매 분획을 시행하여 활성성분을 분리하고 분광학적 분석법을 이용하여 분리한 단일성분이 flavonol 구조인 3,5,7-trihydroxy-2-phenylchromen-4-one (galangin, GLG)임을 확인하였다. 작용기전을 알아보기 위해, Western blot 및 RT-PCR 실험결과, 분리한 flavonol 성분(GLG)의 NO 생성저해 활성은 iNOS mRNA발현을 저해하여 iNOS 효소 단백질의 생성이 억제됨에 기인하는 것으로 확인하였다. 따라서, 고량강 추출물로부터 분리한 flavonol 화합물(GLG)이 iNOS 발현의 억제를 통해 다량의 NO 생산을 저해함으로써, 고량강(Alpinia officinarum)의 NO 과량생성과 관련된 염증성 질환에 대한 응용 가능성이 클 것으로 기대된다.

• 대한치주과학회:학술대회논문집
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• 대한치주과학회 2005년도 제45회 종합학술대회 연제초록
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• pp.153-153
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• 2005

• 대한치주과학회:학술대회논문집
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• 대한치주과학회 2005년도 제45회 종합학술대회 연제초록
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• pp.91-91
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• 2005

• 대한치주과학회:학술대회논문집
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• 대한치주과학회 2004년도 제44회 종합학술대회 연제초록
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• pp.47-47
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• 2004

• 대한치주과학회:학술대회논문집
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• 대한치주과학회 2004년도 제44회 종합학술대회 연제초록
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• pp.135-135
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• 2004

• 대한치주과학회:학술대회논문집
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• 대한치주과학회 2004년도 제44회 종합학술대회 연제초록
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• pp.136-136
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• 2004