• 대한의생명과학회지
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• 제24권3호
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• pp.196-205
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• 2018

Reactive oxygen species (ROS) generated from metabolic reactions cause oxidative DNA damage, which results in oxidative tissue injury. Therefore, there is an increasing demand in the intake of high antioxidant sources in order to maintain a healthy environment in cells. In this study, we investigated the antioxidant and anti-inflammatory activities of Malus domestica (apple), Pyrus communis L. (pear), Daucus carota L. (carrot), Brassica oleracea var. (broccoli), Brassica oleracea var. capitata (cabbage), and Raphanus sativus L. (radish) obtained from the local market. Since these are common fruits and vegetables that are widely consumed, we aimed to investigate their beneficial properties, placing particular emphasis on their antioxidant and anti-inflammatory properties. The samples were processed via an indirect heating method and their properties were compared to their raw forms. Based on DPPH and ABTS assays, processed samples showed better antioxidant activities when compared to raw samples and processed pear samples exhibited the best antioxidant activity. The anti-inflammatory activities of the samples were also investigated in LPS-treated RAW 264.7 cells. mRNA expression of pro-inflammatory mediators and cytokines (iNOS, COX-2, $TNF-{\alpha}$, $IL-1{\beta}$, and IL-6) was assessed using RT-PCR. As expected, processed samples exhibited better iNOS inhibition when compared to their raw forms and processed broccoli and cabbage samples exhibited outstanding anti-inflammatory effects. The samples, up to 1 mg/mL concentration, did not exhibit cytotoxicity against RAW 264.7 cells as demonstrated by cell viability assays. Altogether, processed broccoli and cabbage samples exhibited the strongest anti-inflammatory properties.

• 한국구조물진단유지관리공학회 논문집
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• 제15권6호
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• pp.175-183
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• 2011

우리나라 지반공학 기술의 발달로 지반굴착 공사가 대규모로 수행되고 있으나, 최근 지반굴착시 공사현장 또는 인접구조물 시설물에 직,간접적인 피해를 유발하는 사고가 빈번히 발생하고 있다. 특히 도심지의 지하터널, 도시철도 역사, 대규모 상업시설, 초고층 빌딩의 기초 공사, 대규모 단지의 재개발 등 인접한 곳에 주거시설이나 상업시설이 밀집해 있는 곳에서의 지반굴착 사고가 빈번히 발생하고 있어 경제적인 손실 뿐 아니라 인명피해가 발생하여 사회적인 비용이 급속히 증가하고 있는 실정이다. 따라서 빈번히 발생하는 흙막이 굴착관련 사고를 방지하기 위해서는 여러 유형의 붕괴사고의 원인에 대한 분석이 필요하다. 본 고에서는 최근 발생한 흙막이 굴착공사로 인한 다수의 사고 및 인접시설물에 영향을 끼친 사례를 수집, 분석을 수행하여 사고발생 원인에 대하여 고찰과 개선방안을 제안하였다.

• 스마트미디어저널
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• 제5권1호
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• pp.130-136
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• 2016

본 논문에서 제안하는 실시간 해양 이상조류 모니터링 시스템은 직접적으로 적 녹조의 주원인인 플랑크톤을 감지하는 것이 아니라 적조의 동물성 플랑크톤 특성인 수중 내의 산소 감소를 체크하고, 녹조의 식물성 플랑크톤 특성인 수중 내의 질소 감소를 측정한다. 각각의 특성 체크 및 간접적 요소인 수중 내외의 온도, 조도 센서를 이용하여 실시간 감시하는 모듈을 만들고 모듈은 특정 주기에 맞게 신호를 서버로 전송하여 데이터베이스를 형성하고, 이렇게 수집된 데이터는 해양수산청 적 녹조 기준 데이터와 비교 하여 분석하여 알맞은 형태의 정보로 가공한 뒤 사용자에게 정보를 시각화 하여 제공한다. 기존의 광역적 감지 및 감시 시스템이 아닌 지역적 특성을 갖게 되는, 어업을 하지 않는 시간에도 빠르게 대처 가능한 개인사업 맞춤형 양식장 적 녹조 감시 시스템을 제안하였다.

• 한국산학기술학회논문지
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• 제11권7호
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• pp.2444-2450
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• 2010

본 연구는 공공서비스의 법인화를 위한 사전연구로서, 기업형태의 법인설립을 위한 논리적인 근거를 뒷받침하기 위하여 기존의 지식체계와 공공서비스의 경영방식 등을 비교분석하고 머드사업소의 법인화에 따른 문제점과 이에 대한 극복방안을 제시하는 데, 연구의 주목적을 두고 있다. 연구결과는 다음과 같다. 첫째, 머드사업소가 생산 공급하는 재화는 공공서비스이다. 둘째, 머드사업소의 법인화 방향은 공공성과 기업성, 지역성을 조화시키는 공기업이나공익사업 형태로 접근하는 것이 바람직하다. 셋째, 머드사업소의 법인화에 가장 바람직한 경영방식은 직접경영방식과 간접경영방식, 민간위탁 및 민영화 등 3가지 방식 중에서 간접경영방식이다. 넷째, 본 연구는 머드사업소의 경영관리방식으로서 지방공단, 전액출자형 지방공사, 제3섹터형 지방공사, 제3섹터형기업에 따른 각각의 과제를 보완 내지는 극복할 수 있는 방안들을 제시하고 있다.

• Restorative Dentistry and Endodontics
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• 제26권6호
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• pp.464-484
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• 2001

When restoring a tooth, the dentist tries to choose the ideal material for existing situation. One criterion that is considered is its suitability for restoring coronal strength. As more tooth structure is removed, the cusps are weakened and susceptible to fracture. Further, this increased deformation may cause the formation of intermittent gaps at the margin between the hard tissue and the restoration, facilitating marginal leakage. The improvements in ceramic materials now make it possible for alternatives to amalgams, composites, and cast metal to be of offered for posterior teeth. Of the materials used, ceramics most closely approximates the properties of enamel. The introduction of computer-aided design/computer-aided manufacture(CAD/CAM) systems to restorative dentistry represents a major technological breakthrough. It is possible to design and fabricate ceramic restorations at a single appointment. Additionally, CAD/CAM systems eliminate certain errors and inaccuracies that are inherent to the indirect method and provide an esthetic restoration. The aim of this investigation was to study the loading characteristics of CAD/CAM ceramic inlay and to compare the stress distribution and displacement associated with different designs of cavity(the isthmus width and cavity depth). A human maxillary left first premolar was prepared with standard mesio-occlusal cavity preparation, as recommended by the manufacturer Ceramic inlay was fabricated with CEREC 2 CAD/CIM equipment and cemented into the prepared cavity. Three dimensional model was made by the serial photographic method. The cavity width was varied $\frac{1}{3}$, $\frac{1}{2}$ and $\frac{2}{3}$ of intercuspal distance between buccal and lingual cusp tip. The cavity depth was varied 1.5mm and 2.3mm. So six models were constructed to simulate six conditions. A point load of 500N was applied vertically onto the first node of the lingual slope from the buccal cusp tip. The stress distribution and displacement were solved using ANSYS finite element program(Swanson Analysis System). (omitted)

• Journal of Chest Surgery
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• 제25권7호
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• pp.693-701
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• 1992

Extracorporeal cardiopulmonary bypass[CPB] has been associated with a wide variety of hematologic derangements, including a transient deformation and hemolysis of red blood cells[RBCs], which is supposed to be due to mechanical trauma and/or metabolic alterations. Since membrane integrity is, in part, maintained by energy requiring process, inadequate function of erythrocyte glycolytic pathway, which is inevitalble during CPB, may cause depletion of high energy phosphate pool and result in hemolysis. The authors performed an investigation to assess whether administration of Fructose-l, 6-diphsphate [FDP], which has been known to enhance intracellular glycolytic activities, could counteract erythrocyte hemolytic events caused by CPB. Sixty pateints with cyanotic congenital heart diseases, who underwent open heart surgery under CPB longer than 60 minutes, were randomly divided into two groups depending on whether use of FDP[Group FDP] or not[Group Control]. The age, sex, CPB time, preoperative hemoglobin level, disease entities were all similar[Table 1], and membrane type oxygenators were used in all patients. In Group, FDP, a dose of 250mg/kg body weight of FDP was administered by intravenous dripping every 12 hours from the morning of the operation to postoperative 48 hours, To demonstrate the degree and pattern of hemolysis of erythrocyte, reticulocyte count, indirect /direct bilirubin, haptoglobin, plasma hemoglobin, lactate dehydrogenase were measured every 12 hours from the time of cessation of CPB to 48 hours and RBC morphologic study, osmotic fragility test were done every 24 hours. All parameters revealed less hemolytic in group FDP [Fig. 1~5], though the differences between two groups were not significant, except plasma hemoglobin, lactate dehydrogenase changes. A pattern of sequential changes of plasma hemoglobin, lactate deh-ydrogenase showed the highest level at the time of CPB stop and abrupt decrease in following 24 hours in both groups, and statistically significant differences were demonstrated in group FDP at least for the first 12 hours postoperatively[p<0.05]. The authors conclude that they can expect the benificial effect of FDP on the maintenance of membrane stability of RBC probably by energy enhancement during the shock status of CPB, but FDP could not completely prevent the damaging effect on RBC by cardiopulmonary bypass

• Parasites, Hosts and Diseases
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• 제41권4호
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• pp.243-245
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• 2003

Neospora caninum is an important cause of abortion in cattle, and dogs are its only known definitive host. Its seroprevalence among domestic urban and rural dogs and feral raccoon dogs (Nyctereutes procyonoides koreensis) in Korea was studied by indirect fluorescent antibody test (IFAT) and by the neospora agglutination test (NAT), respectively. Antibodies to N. caninum were found in 8.3% of urban dogs and in 21.6% of dogs at dairy farms. Antibody titers ranged from 1:50 to 1:400. Antibodies to N. caninum were found in six (23%) of 26 raccoon dogs. However, the potential role of raccoon dogs as a source of horizontal transmission of bovine neosporosis needs further investigation. The results of this study suggest that there is a close relationship between N. caninum infection among dairy farm dogs and cattle in Korea. This study reports for the first time upon the seroprevalence of N. caninum infection in raccoon dogs in Korea.

• 한국측량학회지
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• 제28권5호
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• pp.485-491
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• 2010

붕괴된 사면의 정보는 제2차 피해의 발생우려가 있기 때문에 신속 정확하게 지형자료를 획득할 수 있어야 하고, 필요에 따라서 접근하지 않고 간접적인 측량방식으로 보다 효율적으로 표현할 수도 있어야 한다. 따라서 본 연구에서는 무인 헬리콥터 사진촬영시스템을 이용하여 붕괴된 도로 절개지 사면에 근접하여 상공에서 정지비행으로 대상지역의 영상을 촬영하였다. 그리고, 무타켓 Total Station에 의해 관측된 점과 영상해석에 의해 분석된 좌표점 10개와 비교한 결과 절대치 평균값은 X축 방향으로 평균 0.056m, Y축 방향으로 0.082m, Z축 방향으로 0.066m으로 나타났다. 또한, 검사점 10점에 대한 오차의 RMSE는 X축 방향으로 0.015636m, Y축 방향으로 0.021319m, Z축 방향으로 0.018734m로 나타났다. 따라서, 이러한 절개지 붕괴사면의 지형에 대하여 관계형 영상정합방법에 의해 접근하지 못하는 위험지역에서의 사면 범위와 각사면의 종 횡단면을 나타낼 수가 있었다.

• 대한미생물학회지
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• 제22권3호
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• pp.197-208
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• 1987

Chlamydia trachomatis has now shown that this interesting intracellular parasite is a cause of nongonococcal urethritis, infantile pneumonia, pelvic inflammatory disease and epididymitis, in addition to lymphogranuloma venerum and inclusion conjunctivitis. There are several diagnostic methods for C. trachomatis, but the method using monoclonal antibody is the most sensitive and specific. The hybride cell were prepared by fusion of myeloma cell($P_3X_{63}\;Ag_8{\cdot}V_{653}$) of mouse and lymphocyte of mouse(BALB/c) that were immunized with formalin killed C. trachomatis serotype D. The cell mixtures after fusion were dispensed into 640 wells of the 96 well culture plates and continuously cultured in HAT medium for 2 weeks. The supernatants of culture media in 83(13%) wells were reacted with C. trachomatis, which were determined by enzyme-linked immunosorbent assay in 96 well microplate. The clones that secreted antibody to C. trachomatis were cloned by limiting dilution. Only six monoclones secreted antibody to C. trachomatis. The antibody titer of ascitic fluid that collected from same BALB/c mice bearing hybridoma cells was above 1:100,000. These monoclonal antibodies that were IgG reacted with elementary and reticulate bodies of all serotypes(Ba, D, E, F, G, H, J and LGV type-I) using ELISA and indirect immunofluorescence stain, but there were no cross reaction with other bacteria(coagulase negative Staphylococcus, Proteus and E. coli). We concluded these six monoclones secreted the same monoclonal antibody to C. trachomatis. The sensitivity and specificity of the monoclonal antibody compared with Microtrak(confirmatory test of C. trachomatis, Syva) was 100%, respectively.

• 한국어병학회지
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• 제32권1호
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• pp.29-35
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• 2019

2015년부터 2017년까지 우리나라 넙치 양식장에서 발생하는 폐사피해를 표본조사를 통해 모니터링 하였다. 표본어가는 전국의 수산질병관리원과 지속적으로 거래하고 있으며 현장방문을 통해 설문이 가능한 양식어가 중 층화구조분석을 통해 선정 하였다. 수산질병관리사 조사요원의 직접적인 현장방문을 통한 설문 조사를 수행하여 객관적으로 양식현황 및 폐사 현황을 파악하였다. 조사기간 중 24.78%/2015년, 30.19%/2016년 그리고 21.59%/2017년의 누적 폐사율을 나타내었고, 감염성 질병에 의한 폐사는 스쿠티카증(2015-2017년 평균 누적 폐사율: 56.7%)과 바이러스성출혈성 패혈증 감염(추정)(2015-2017년 평균 누적 폐사율: 8.9%)에 의한 피해가 높은 것으로 나타났으며, 제주에서는 여윔증(2016-2017년 평균 누적 폐사율: 10.3%)에 의한 피해가 높게 나타났다.