• Asian-Australasian Journal of Animal Sciences
• /
• v.13 no.4
• /
• pp.464-469
• /
• 2000

Experiments were conducted to determine the effects of soybean meal (SBM) as a source of protein and sago meal (SM) as a source of carbohydrate on in situ and in vivo digestibility of dietary components in four male goats (Kambing Katjang) and four male sheep (Malin) weighing 25-35 kg. Rumen volume, as well as rumen fluid dilution rate were also determined. The animals were housed in single pens with individual feeding and drinking troughs and each animal was fitted with a rumen fistula. They were fed two diets : chopped rice straw+200 g soybean meal (SBM), and chopped rice straw+190 g soybean meal+300 g sago meal (SBM+SM). Rice straw was offered ad libitum. The supplements were isonitrogenous (80 g crude protein/animal/d), but the proportions of dry matter (DM), organic matter (OM), crude fibre (CF), neutral detergent fibre (NDF) and acid detergent fibre (ADF) were lower in the SBM supplement (191, 165, 11, 40, 15 g/animal/d for DM, OM, CF, NDF and ADF, respectively) than in the SBM+SM supplement (445, 423, 25, 102, 38 g/animal/d for DM, OM, CF, NDF and ADF, respectively). Two animals from each species were fed either supplement in a cross-over design in two periods. Each period lasted for four weeks. In situ and in vivo digestibility studies were carried out, followed by the determination of rumen volume and rumen fluid dilution rate. The results showed that straw DM and total DM intakes of goats (average of $48.7g/kg\;W^{0.75}$, $72.7g/kg\;W^{0.75}$, respectively) were significantly (p<0.01) higher than sheep (average of $3.56g/kg\;W^{0.75}$, $61.6g/kg\;W^{0.75}$, respectively), but OM, N and GE intakes were not significantly different between the two animal species. When the effect of supplements was compared, animals fed SBM+SM supplement had significantly (p<0.001) higher DM, OM and GE intakes than animals fed SBM supplement. Potential degradabilities of rice straw DM were significantly (p<0.01) higher in goats (average of 48.8%) than in sheep (average of 46.1 %). The supplements had no significant effect on the potential degradabilities of DM, OM and NDF, but they had a significant (p<0.05) effect on the degradation rates of DM and NDF. The addition of sago meal in the diet reduced the degradation rates of DM and NDF of rice straw in the rumen. Potential degradability of DM of soybean meal was not significantly different between animal species as well as between supplements. Sago meal was highly degradable. At 24 h of incubation in the rumen, 90-95% of DM loss was observed. There was a significant interaction between animal species and supplements in the in vivo digestibility of ADF and GE. In animals fed SBM supplement, the in vivo digestibility of ADF was significantly (p<0.05) higher in goats ($50.6{\pm}4.22%$) than in sheep ($44.4{\pm}3.21%$), but digestibility of GE was significantly (p<0.05) higher in sheep ($70.2{\pm}1.93%$) than in goats ($63.0{\pm}3.07%$). The digestibility values of CP and OM were significantly (p<0.05) higher in sheep when compared to goats. Animals fed SBM+SM supplement showed significantly (p<0.05) higher DM and OM digestibility values than animals fed SBM supplement, but digestibility values of CP were significantly (p<0.05) higher in animals fed SBM supplement. Differences in in vivo digestibility values of CF and NDF were not significantly different between animal species or supplements. Water intake, rumen volume ($1/kg\;W^{0.75}$), rumen fluid dilution rate and mean retention time were similar between the two animal species. However, rumen fluid dilution rate and mean retention time was significantly (p<0.01) affected by supplements. Animals fed SBM+SM had faster rumen fluid dilution rate and consequently shorter mean retention time.

• Korean Journal of Environment and Ecology
• /
• v.26 no.2
• /
• pp.219-232
• /
• 2012

The purpose of this study was to find characteristics of $Pinus$ $densiflora$ forests in previous records and cultural landscape regions and to establish a management method for recovery of $P.$ $densiflora$ landscape. A total of 16 landscape paintings were analyzed to study $P.$ $densiflora$ forests in Inwangsan(Mt.) shown in figure data. As a result of analyzing figure data, $P.$ $densiflora$ $forests$ were found to be distributed mainly in the ridge line of east slope side of Inwangsan(Mt.) from landscape paintings of Jung Seon, Jang Si Heung and Gang Hee Un. In order to analyze the landscape of $P.$ $densiflora$ forests shown in photograph data on Inwangsan(Mt.), photographs of Inwangsan(Mt.) since late 1800s were searched and a total of 24 photographs were used for analysis. As a result of photographic analysis, $P.$ $densiflora$ forests were found to be distributed mainly in the low area and ridge line of western slope side of Inwangsan(Mt.). As a result of analyzing the distribution status of 10 cultural heritages of Inwangsan(Mt.), cultural heritages in the mountain were mainly established with rocks and $P.$ $densiflora$ forests as the background and are establishing their landscape not only as a form of cultural heritage but also including the natural landscape in the background. A method of restoring cultural landscape of P. densiflora forests was necessary. As a result of analyzing 26 rock beds of Inwangsan(Mt.), most of $P.$ $densiflora$ forests were distributed nearby rock beds since the past. Among such regions, restoration and management of introduced species in the accumulated soil regions to $P.$ $densiflora$ forests were deemed necessary. As a result of analyzing current vegetation of $P.$ $densiflora$ forest areas of the past, 51.87% of areas maintained $P.$ $densiflora$ forest and 25.80% were changed to artificial forests. Most of low areas of the mountain were turned into urban area (10.16%). Management type for restoration of $P.$ $densiflora$ landscape of Inwangsan(Mt.) was classified into preservation, $P.$ $densiflora$ restoration and maintenance. Preservation management was configured as $P.$ $densiflora$ distribution and rocky area distribution. The targets of $P.$ $densiflora$ restoration were indigenous broad-leaf trees, vegetation areas with introduced species and damaged regions within the mountain. The targets of maintenance were urban area in the low area of Inwangsan(Mt.) and military base at the top of the mountain.

• Korean journal of applied entomology
• /
• v.53 no.4
• /
• pp.427-433
• /
• 2014

To investigate the biological control of Aulacorthum solani, a comparative analysis was carried out using an indigenous natural enemy, Ephedrus plagiator, and an exotic parasitoid, Aphidius ervi. Lifespan, spawning periods, number of mummies, number of offspring, and developmental periods of the two parasitoids on Aulacorthum solani and Acyrthosiphon pisum were studied at 15, 20, 25, and $30^{\circ}C$ with a photoperiod 16 h (L): 8 h (D). The lifespan of E. plagiator (5.3 days) was relatively higher than that of A. ervi (2.3 days) when these parasitoids were reared on A. solani at $15^{\circ}C$. Similarly, the spawning period of E. plagiator (5.3 days) was longer than that of A. ervi (2.2 days). When the two aphid parasitoids were provisioned with A. solani, the numbers of E. plagiator mummies at 15, 20, 25, and $30^{\circ}C$ were 71.5, 41, 15.7, and 7.7, respectively, whereas at the same temperatures, the numbers of A. ervi mummies were 22.1 16.3, 6.2, and 0.4, respectively. In contrast, when provision with A. pisum, the numbers of E. plagiator mummies at 15, 20, 25, and $30^{\circ}C$ were 70.3, 69.8, 34.3, and 8.4, whereas the numbers of A. ervi mummies were 93.4, 71.2, 34.8, and 14.5, respectively. The numbers of E. plagiator offspring emerging at 15, 20, 25, and $30^{\circ}C$ were 42.1, 36, 11.6, and 0, whereas the numbers of A. ervi offspring emerging were relatively lower at 19.6, 13.5, 3.7, and 0.1, respectively. By comparing these results, it can be concluded that E. plagiator is a more efficient parasitoid of A. solani, whereas A. ervi is more efficient on A. pisum.

• Korean Journal of Ecology and Environment
• /
• v.53 no.3
• /
• pp.241-254
• /
• 2020

Three introduced fish species, Japanese white crucian carp (Carassius cuvieri Temminck and Schlegel, 1846), bass (Micropterus salmoides Lacepède, 1802) and bluegill (Lepomis macrochirus Rafinesque, 1819), are dominant fishes in Korean freshwater ecosystem. In this study, we analyzed habitat environment conditions of these three species and their impacts to fish communities in streams across South Korea. Fish community data were obtained from the database of the Stream/River Ecosystem Survey and Health Assessment program maintained by the Ministry of Environment and the National Institute of Environmental Research, Korea. Our results showed that species richness and Shannon diversity of fish were higher at the presence sites of introduced fish than at the absence sites. However, when the abundance of these introduced fish species was increased, the species richness and abundance of fish were decreased. An association analysis showed that the introduced fish species had a low similarity in their appearance with some indigenous fishes such as Siniperca scherzeri and Channa argus and some endemic fishes of Korea such as Zacco koreanus, Sarcocheilichthys variegatus wakiyae, and Acheilognathus yamatsutae. In addition, the introduced fish species had a low appearance similarity with a large number of fishes in their association networks. Finally, our results presented that these introduced fish species influenced the negative impacts to the stream fish communities, and they were potential risk factors for fish community in Korean freshwater ecosystem. Therefore, it is necessary that continuous monitoring and establishment of management strategy for introduced fish species to preserve fish resource and biodiversity in the Korean streams.

• Korean Journal of Environmental Agriculture
• /
• v.26 no.1
• /
• pp.62-68
• /
• 2007

Concerns about endocrine disrupting chemicals emitted from humans and animals have been increased because these compounds are detected at very low levels in environment and adversely affect on indigenous fauna. To date, there is little information regarding the concentration of these compounds in animal wastes. In this study, the female hormones, $17\beta-estradiol$ (E2), estrone (E1) and estriol, were measured to provide baseline data in animal wastes. Samples were collected from animal waste storage, methane digester and sludge separated wastewater and analyzed by gas chromatography-mass spectrometry. To measure the mass ratios of estrogen to macronutrients, nitrogen and phosphorous were also determined. Sample collected from animal waste storage had the highest estrogen concentration (98.7 ${\mu}g/L$), while sludge separated wastewater had the lowest concentration (3.4 ${\mu}g/L$). The mean concentrations of E2 and E1 in waste storage sample were (6.8 ${\mu}g/L$) and (68.7 ${\mu}g/L$), respectively. In sludge separated wastewater, the mean concentration of both E2 and E1 were reduced to (2.6 ${\mu}g/L$) and (1.9 ${\mu}g/L$), respectively. However, estriol was not detected in any of the samples collected. Mean ratios of E2 and E1 to macronutrients were significantly different between the methane wastewater and sludge separated wastewater owing to elimination of solid particles.

• Natural Product Sciences
• /
• v.8 no.1
• /
• pp.1-15
• /
• 2002

An International Cooperative Biodiversity Group (ICBG) program based at the University of Illinois at Chicago initiated its activities in 1998, with the following specific objectives: (a) inventory and conservation of of plants of Cuc Phuong National Park in Vietnam and of medicinal plants of Laos; (b) drug discovery (and development) based on plants of Vietnam and Laos; and (c) economic development of communities participating in the ICBG project both in Vietnam and Laos. Member-institutions and an industrial partner of this ICBG are bound by a Memorandum of Agreement that recognizes property and intellectual property rights, prior informed consent for access to genetic resources and to indigenous knowledge, the sharing of benefits that may arise from the drug discovery effort, and the provision of short-term and long-term benefits to host country institutions and communities. The drug discovery effort is targeted to the search for agents for therapies against malaria (antimalarial assay of plant extracts, using Plasmodium falciparum clones), AIDS (anti-HIV-l activity using HOG.R5 reporter cell line (through transactivation of the green fluorescent protein/GFP gene), cancer (screening of plant extracts in 6 human tumor cell lines - KB, Col-2, LU-l, LNCaP, HUVEC, hTert-RPEl), tuberculosis (screening of extracts in the microplate Alamar Blue assay against Mycobacterium tuberculosis $H_{37}Ra\;and\;H_{37}Rv),$ all performed at UIC, and CNS-related diseases (with special focus on Alzheimer's disease, pain and rheumatoid arthritis, and asthma), peformed at Glaxo Smith Kline (UK). Source plants were selected based on two approaches: biodiversity-based (plants of Cuc Phuong National Park) and ethnobotany-based (medicinal plants of Cuc Phuong National Park in Vietnam and medicinal plants of Laos). At mc, as of July, 2001, active leads had been identified in the anti-HIV, anticancer, antimalarial, and anti- TB assay, after the screening of more than 800 extracts. At least 25 biologically active compounds have been isolated, 13 of which are new with anti-HIV activity, and 3 also new with antimalarial activity. At GSK of 21 plant samples with a history of use to treat CNS-related diseases tested to date, a number showed activity against one or more of the CNS assay targets used, but no new compounds have been isolated. The results of the drug discovery effort to date indicate that tropical plant diversity of Vietnam and Laos unquestionably harbors biologically active chemical entities, which, through further research, may eventually yield candidates for drug development. Although the substantial monetary benefit of the drug discovery process (royalties) is a long way off, the UIC ICBG program provides direct and real-term benefits to host country institutions and communities.

• Economic and Environmental Geology
• /
• v.41 no.6
• /
• pp.645-655
• /
• 2008

The Cr(VI) concentrations at the shallow aquifer well (MPH-0-1) of the Moonpyung groundwater monitoring station were in the range of 0.5 to 3.1 mg/L exceeding 10 to 62 times the guideline for drinking-water quality, indicating continuous contamination. However, Cr was not detected at the deep bedrock well and the other subsidiary monitoring wells except for MPH-1 and 6. Cross-correlation analyses were conducted for rainfall and groundwater level time series, resulting in the mean time of recharge after precipitation events to be 5.6 days. For rainy season, the water level was raised and the Cr(VI) concentration was several times lower than that during dry season at well MPH-0-1 well. Correlation of the Cr(VI) concentration with the groundwater-level showed that the Cr(VI) reduction was closely related with the groundwater-level rise in the well. However, the groundwater level rise during high water season induced the lateral migration of the Cr(VI)-contaminated groundwater at well MPH-4. We enriched and isolated a chromium reducing bacteria, Enterobacter aerogenes, from the Cr(VI)-contaminated groundwater in the wells MPH-0-1 and MPH-1. The bacteria may play an important role for immobilizing Cr(VI) in the Cr(VI)-contaminated groundwater. Therefore, the migration of the contaminant (Cr(VI) must has been restricted because of the natural attenuation by microbial reduction of Cr(VI) in the groundwater. This research suggests that the bioremediation of the Cr(VI)-contaminated groundwater by the indigenous bacteria may be feasible in the Cr(VI) contaminated groundwater.

• Research in Plant Disease
• /
• v.24 no.2
• /
• pp.145-152
• /
• 2018

During 2015-2017, we surveyed the incidence of viral infections of tomato and paprika growing in greenhouses in Cherwon province, Korea. In 2015 and 2016, we collected leaves and fruits from tomato and paprika plants growing in greenhouses. We detected viruses in the samples collected using specific primer sets for Broad bean wilt virus 2 (BBWV2), Cucumber mosaic virus (CMV), Pepper mild mottle virus (PMMoV), Pepper mottle mosaic virus (PepMoV), and Tomato spotted wilt virus (TSWV). We detected PMMoV, CMV, and TSWV in the samples, and CMV and TSWV were the most prevalent. For the prevention of future viral diseases, we then surveyed the routes of infection by these viruses in tomato and paprika plants growing in greenhouses in Cherwon province in 2017. Leaf and fruit samples were collected from seedlings and crops two and four months after transplanting into greenhouses. As a result, we found that TSWV was transferred from seedlings to plants, and outbreaks of the virus occurred at the early stage of cultivation. On the other hand, we found that CMV was a virus indigenous to the soil of some towns in Cherwon province, and thus outbreaks of this virus occurred at the middle stage of cultivation.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
• /
• 2003.10a
• /
• pp.111-124
• /
• 2003

Processes that cause immobilization of contaminants in soil are of great environmental importance because they may lead to a considerable reduction in the bioavailability of contaminants and they may restrict their leaching into groundwater. Previous investigations demonstrated that pollutants can be bound to soil constituents by either chemical or physical interactions. From an environmental point of view, chemical interactions are preferred, because they frequently lead to the formation of strong covalent bonds that are difficult to disrupt by microbial activity or chemical treatments. Humic substances resulting from lignin decomposition appear to be the major binding ligands involved in the incorporation of contaminants into the soil matrix through stable chemical linkages. Chemical bonds may be formed through oxidative coupling reactions catalyzed either biologically by polyphenol oxidases and peroxidases, or abiotically by certain clays and metal oxides. These naturally occurring processes are believed to result in the detoxification of contaminants. While indigenous enzymes are usually not likely to provide satisfactory decontamination of polluted sites, amending soil with enzymes derived from specific microbial cultures or plant materials may enhance incorporation processes. The catalytic effect of enzymes was evaluated by determining the extent of contaminants binding to humic material, and - whenever possible - by structural analyses of the resulting complexes. Previous research on xenobiotic immobilization was mostly based on the application of $^{14}$ C-labeled contaminants and radiocounting. Several recent studies demonstrated, however, that the evaluation of binding can be better achieved by applying $^{13}$ C-, $^{15}$ N- or $^{19}$ F-labeled xenobiotics in combination with $^{13}$ C-, $^{15}$ N- or $^{19}$ F-NMR spectroscopy. The rationale behind the NMR approach was that any binding-related modification in the initial arrangement of the labeled atoms automatically induced changes in the position of the corresponding signals in the NMR spectra. The delocalization of the signals exhibited a high degree of specificity, indicating whether or not covalent binding had occurred and, if so, what type of covalent bond had been formed. The results obtained confirmed the view that binding of contaminants to soil organic matter has important environmental consequences. In particular, now it is more evident than ever that as a result of binding, (a) the amount of contaminants available to interact with the biota is reduced; (b) the complexed products are less toxic than their parent compounds; and (c) groundwater pollution is reduced because of restricted contaminant mobility.

• Korean Journal of Soil Science and Fertilizer
• /
• v.30 no.3
• /
• pp.234-241
• /
• 1997

The effects of bulk densities(${\rho}_b$) on saturated hydraulic conductivity (Ksat) and solute elution patterns were investigated from five different bulk densities ranging from $1.1Mg/m^3$ to $1.5Mg/m^3$ with each increment of $0.1Mg/m^3$. The hydraulic conductivities observed were divided into two stages: (1) a linearly decrease with increase in bulk density up to $1.4Mg/m^3$, (2) a steady state where the bulk density is greater than $1.4Mg/m^3$. Using the saturated hydraulic conductivity at the steady state, we figured out the equation describing the correlation between bulk densities(${\rho}_b$) and saturated hydraulic conductivity(Ksat) as follows: $Ksat=-19.2({\rho}_b{^2})+6{\rho}_b+15.5$, (r=0.985). Electrical conductivity(EC) measured from the leachate of the soil column showed that EC at the same pore volume were decreased with an increase in the bulk density from $1.2g/cm^3$, $1.5g/cm^3$, as shown in the time taken to collect the same pore volume at each respective bulk density. The maximum relative concentrations (C/Co=1) from the breakthrough curves for the anions of $Cl^-$, $NO_3{^-}$ and $SO_4{^{2-}}$, which are weakly adsorbed on the soil particles, moved to the right of the graph, while a distinctive retardation occurs at the bulk density between $1.3Mg/m^3$ and $1.4Mg/m^3$. The time taken to recover about 90% of indigenous sulphate was approximately twice as those of chloride and nitrate, resulting in slightly stronger adsorption characteristics for sorption sites on the soil surface. Thus, we can conclude that the salt accumulation in green house soil might be significantly influenced by it's bulk density at the soil depth, as well as the adsorption capacity of ions for the sorption sites in soils.