DOI QR코드

DOI QR Code

Survey of the Routes and Incidence of Viral Infection of Tomato and Paprika Growing in Greenhouses in Cherwon Province, Korea During 2015-2017

2015-2017 철원군 시설재배 과채류(토마토와 파프리카)의 바이러스병 발생 현황 및 감염경로 조사

  • 길형배 (철원군 농업기술센터) ;
  • 강민지 ((재)철원플라즈마산업기술연구원) ;
  • 최원석 ((재)철원플라즈마산업기술연구원) ;
  • 김중일 ((재)철원플라즈마산업기술연구원) ;
  • 미사 보 판 (서울대학교 농생명공학부) ;
  • 임지희 (철원군 농업기술센터) ;
  • 김미경 (철원군 농업기술센터) ;
  • 박미리 ((재)철원플라즈마산업기술연구원)
  • Received : 2018.05.29
  • Accepted : 2018.06.15
  • Published : 2018.06.30

Abstract

During 2015-2017, we surveyed the incidence of viral infections of tomato and paprika growing in greenhouses in Cherwon province, Korea. In 2015 and 2016, we collected leaves and fruits from tomato and paprika plants growing in greenhouses. We detected viruses in the samples collected using specific primer sets for Broad bean wilt virus 2 (BBWV2), Cucumber mosaic virus (CMV), Pepper mild mottle virus (PMMoV), Pepper mottle mosaic virus (PepMoV), and Tomato spotted wilt virus (TSWV). We detected PMMoV, CMV, and TSWV in the samples, and CMV and TSWV were the most prevalent. For the prevention of future viral diseases, we then surveyed the routes of infection by these viruses in tomato and paprika plants growing in greenhouses in Cherwon province in 2017. Leaf and fruit samples were collected from seedlings and crops two and four months after transplanting into greenhouses. As a result, we found that TSWV was transferred from seedlings to plants, and outbreaks of the virus occurred at the early stage of cultivation. On the other hand, we found that CMV was a virus indigenous to the soil of some towns in Cherwon province, and thus outbreaks of this virus occurred at the middle stage of cultivation.

철원지역의 주요 과채류인 토마토와 파프리카를 대상으로 바이러스병 발생 현황을 조사하고 바이러스병에 대한 방제를 위해 2015년부터 2017년까지 시설 하우스를 중심으로 조사를 실시하였다. 2015년-2016년에는 7-9월에 채집을 진행하였고, 채집시료는 시설 하우스에서 재배되고 있는 토마토와 파프리카의 잎과 과실이었다. 파프리카와 토마토를 포함하는 가지과 작물에 주요 바이러스병을 일으키는 5종의 바이러스인 Pepper mild mottle virus (PMMoV), Cucumber mosaic virus (CMV), Pepper mottle virus (PepMoV), Broad bean wilt virus 2 (BBWV2), Tomato spotted wilt virus (TSWV)에 대하여 유전자 진단법을 이용하여 채집시료를 검정하였다. 그 결과, PMMoV, CMV, 그리고 TSWV가 검출되었으며, 이들 중, CMV와 TSWV에 의한 바이러스병이 대부분(2015년에 파프리카에서 CMV 검정률 50.0%, TSWV 12.2%, 토마토에서 CMV 25.5%, TSWV 38.3% 이고, 2016년에 파프리카에서 CMV 45.9%, TSWV 45.5%, 토마토에서 CMV 23.8%, TSWV 47.6%)을 차지하고 있었다. 이 두 바이러스의 조기예방을 위한 발생 경로를 추적하기 위하여 2017년에는 작기별(육묘, 정식중기, 정식후기)로 채칩 시기를 나누어 바이러스 검정을 실시하였다. 조사 결과, TSWV는 육묘에 감염되어 외부에서 철원군내에 유입되어 초기 병발생율이 높았고, CMV는 철원군내 농가에 토착화되어 작기가 중반으로 넘어갈수록 병발생율이 높게 나타난 것으로 확인되었다. 또한 2017년도 작기별 바이러스병 발생 현황 조사 결과, TSWV에 의한 바이러스병 발생율이 육묘에서 검출 이후 점점 감소하는 현상은 본 연구를 통해 육묘때 조기진단으로 인한 바이러스 검출 농가의 조기 대응 및 지속적인 관찰이 바이러스병의 확산 방지에 중요한 역할을 한 결과로 보인다.

Keywords

References

  1. Cho, J. D., Kim, J. S., Lee, S. H. and Choi, G. S. 2007a. Viruses and symptoms on peppers, and their infection types in Korea. Res. Plant Dis. 13: 75-81. (In Korean) https://doi.org/10.5423/RPD.2007.13.2.075
  2. Cho, J. D., Lee, S. H., Kim, J. S., Choi, G. S., Chung, B. N. 2007b. Study of incidence on peppers viruses in Korea. BioWave 9(11): 1. (In Korean)
  3. Choi, G. S., Kim, J. H., Lee, D. H., Kim, J. S. and Ryu, K. H. 2005. Occurrence and distribution of viruses infecting pepper in Korea. Plant Pathol. J. 21: 258-261. https://doi.org/10.5423/PPJ.2005.21.3.258
  4. Choi, G. S., Kim, J. H., Ryu, K. H., Choi, J. K., Chae, S. Y., Kim, J. S., et al. 2002. First report of tobacco mild green mosaic virus infecting pepper in Korea. Plant Pathol. J. 18: 323-327. https://doi.org/10.5423/PPJ.2002.18.6.323
  5. Choi, H. S., Lee, S. H., Kim, M. K., Kwak, H. R., Kim, J. S., Cho, J. D. et al. 2010. Occurrence of virus diseases on major crops in 2009. Res. Plant Dis. 16: 1-9. https://doi.org/10.5423/RPD.2010.16.1.001
  6. Francki, R. I. B., Mossop, D. W. and Hatta, T. 1979. Cucumber mosaic virus. CMI/AAB Descriptions of Plant Viruses 213.
  7. Im, K. H., Chung, B. K., Yoon, J. Y. and Green, S. K. 1991. A survey on viruses infecting peppers (Capsicum annum) in Korea by micro-plate method of enzyme-linked immunosorbent assay (ELISA). Korean J. Plant Pathol. 7: 251-256.
  8. Ji, J., Oh, T. K., Lee, H. J., Kim, S. H., Rajangam, U., Kim, S. C., et al. 2008. Molecular characterization of tomato infecting Tobacco leaf curl geminivirus isolated from Jeju island. Res. Plant Dis. 14: 238. (Abstract)
  9. Kang, M., Seo, J. K., Choi, H., Choi, H. S. and Kim, K. H. 2016. Establishment of a simple and rapid gene delivery system for cucurbits by using engineered Zucchini Yellow Mosaic Virus. Plant Pathol. J. 32: 70-76. https://doi.org/10.5423/PPJ.NT.08.2015.0173
  10. Kataya, A. R. A., Stavridou, E., Farhan, K. and Livieratos, I. C. 2008. Nucleotide sequence analysis and detection of a Greek isolate of tomato chlorosis virus. Plant Pathol. 57: 819-824. https://doi.org/10.1111/j.1365-3059.2008.01845.x
  11. Kim, J. S., Kim, S. K., Lee, S. H. and Lee, M. W. 1990. A pepper vein chlorosis virus causing stem necrosis and vein chlorosis on red pepper in Korea. Korean J. Plant Pathol. 6: 376-381.
  12. Kim, J. S., Lee, S. H., Choi, H. S., Kim, M. K., Kwak, H. R., Kim, J. S. et al. 2012. 2007-2011 characteristics of plant virus infections on crop samples submitted from agricultural places. Res. Plant Dis. 18: 277-289. https://doi.org/10.5423/RPD.2012.18.4.277
  13. Kim, J. S., Lee, S. H., Choi, H. S., Kim, M. K., Kwak, H. R., Nam, M. et al. 2011. Occurrence of virus diseases on major crops in 2010. Res. Plant Dis. 17: 334-341. (In Korean) https://doi.org/10.5423/RPD.2011.17.3.334
  14. Kwak, H. R., Kim, M. K., Nam, M., Kim, J. S., Kim, K. H., Cha, B. and Choi, H. S. 2013. Genetic composition of broad bean wilt virus 2 infecting red pepper in Korea. Plant Pathol. J. 29: 274-284. https://doi.org/10.5423/PPJ.OA.12.2012.0190
  15. Lee, M. Y., Song, Y. S. and Ryu, K. H. 2011. Development of infectious transcripts from full-length and GFP-tagged cDNA clones of pepper mottle virus and stable systemic expression of GFP in tobacco and pepper. Virus Res. 155: 487-494. https://doi.org/10.1016/j.virusres.2010.12.004
  16. Lee, S. H., Lee, J. B., Kim, S. M., Choi, H. S., Park, J. W., Lee, J. S. et al. 2004. The incidence and distribution of viral diseases in pepper by cultivation types. Res. Plant Dis. 10: 231-240. https://doi.org/10.5423/RPD.2004.10.4.231
  17. Mun, H. Y., Park, M. R., Lee, H. B. and Kim, K. H. 2008. Outbreak of cucumber mosaic virus and tomato spotted wilt virus on bell pepper grown in Jeonnam Province in Korea. Plant Pathol. J. 24: 93-96. https://doi.org/10.5423/PPJ.2008.24.1.093
  18. Palukaitis, P. and Garcia-Arenal, F. 2003a. Cucumber mosaic virus. CMI/AAB Descriptions of Plant viruses 400.
  19. Palukaitis, P. and Garcia-Arenal, F. 2003b. Cucumoviruses. Adv. Virus Res. 62: 241-323.
  20. Panno, S., Ferriol, I., Rangel, E. A., Olmos, A., Han, C. G., Martinelli, F. et al. 2014. Detection and identification of Fabavirus Species by one-step RT-PCR and multiplex RT-PCR. J. Virol. Methods 197: 77-82. https://doi.org/10.1016/j.jviromet.2013.12.002
  21. The Korean Society of Plant Pathology. 2009. List of plant diseases in Korea. 5th ed. The Korean Society of Plant Pathology, Seoul, Korea. 853 pp. (In Korean)