• Tuberculosis and Respiratory Diseases
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• v.70 no.2
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• pp.125-131
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• 2011

Background: The clinical manifestation of $M.$ $tuberculosis$ infection ranges from asymptomatic latent infection, to focal forms with minimal symptoms and low bacterial burdens, and finally to advanced tuberculosis (TB) with severe symptoms and high bacillary loads. We investigated the diagnostic sensitivity of the whole-blood interferon-${\gamma}$ release assay according to the wide spectrum of clinical phenotypes. Methods: In patients diagnosed with active TB that underwent $QuantiFERON^{(R)}$ (QFT) testing, the QFT results were compared with patients known to be infected with pulmonary tuberculosis (P-TB) and extra-pulmonary TB (EP-TB). In addition, the results of the QFT test were further analyzed according to the radiographic extent of disease in patients with P-TB and the location of disease in patients with EP-TB. Results: There were no statistical differences in the overall distribution of QFT results between 177 patients with P-TB and 84 patients with EP-TB; the positive results of QFT test in patients with P-TB and EP-TB were 70.1% and 64.3%, respectively. Among patients with P-TB, patients with mild extents of disease showed higher frequency of positive results of QFT test than that of patients with severe form (75.2% vs. 57.1%, respectively; p=0.043) mainly due to an increase of indeterminate results in severe P-TB. Patients with TB pleurisy showed lower sensitivity by the QFT test than those with tuberculous lymphadenitis (48.8% vs. 78.8%, respectively; p=0.019). Conclusion: Although QFT test showed similar results between overall patients with P-TB and EP-TB, individual sensitivity was different according to the radiographic extent of disease in P-TB and the location of disease in EP-TB.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.2
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• pp.177-184
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• 1991

Soybean cultivars for bean sprouting, and for cooking with rice are related closely with Korean dietary tradition, but their yields have been kept in low level. The yielding responses of Danyup, a cultivar for bean sprouting and Gumjung, a cultivar for cooking with rice were compared with Paldal as standard cultivar in planting density within 10 to 80 plants per ㎡. Yield of Paldal increased as planting density increased from 10 plants up to 80 plant/㎡. But, Danyup reached at threshold as planting density reached up to 40 plants/㎡. Gumjung did not have significant difference in yield within planting density. Danyup had the highest yield, 445 kg/l0a at 80 plants/㎡. The highest yield of Paldal was 406kg/10a at 40 plants /㎡ while Gumjung had 373kg/ l0a at 40 plants. The yield responses of cultivars were influenced by canopy structure and light utilization. Paldal had short and straight canopy with determinate growth habit responded well at the heavy density, and had 6.2 in critical LA!. But Gumjung, tall and dense canopy with indeterminate growth habit had limited utilization of light within canopy with 5.4 in critical LAI and high rate of lodging. Gumjung was concluded as a low yielding cultivar, since it did not have good response to increasing source size and sink number.

• Korean Journal of Veterinary Research
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• v.32 no.3
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• pp.381-387
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• 1992

Gill epithelia of normal rainbow trout fingerlings and abnormal ones suffering bacterial gill disease by experimental infection were examined by transmitting electron microscopy (TEM) and scanning electron microscopy (SEM). TEM observations revealed that Flavobacterium branchiophila consisted of slender rods measuring 0.5 by 5 to $8{\mu}m$, and they had which were long, thin, flexible filaments measuring approximately 4 nm by $1{\mu}m$, and packed together to organize into bundles. Morphological alterations of the diseased epithelia started at hypertrophy of the lamellar epithelium. F branchiophila attached to the gill surface of infected fish through pili with a regular distance, and did not invade into gill tissue. In SEM observations, normal surface ultrastructure of epithelial cell in the outermost layer were characterized by a typical labyrinth-like structure branching and anastomosing microridges on the cell surface. Hyperplastic lesions in experimentally infected gill were most serious at near the tips. Each filament exhibited a club-like, and fusion between the filaments was sometimes observed at their tips. On the surface of gill filaments, thread-like bacterial cells attached and were entangled. The bacterial cells almost covered the surface. After immersion in 5 % NaCl, the cell of F branchiophila, however, appeared to be indeterminate shape.

• Tuberculosis and Respiratory Diseases
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• v.69 no.4
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• pp.271-278
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• 2010

Background: Recent studies have demonstrated that the epidermal growth factor receptor (EGFR) genotype is the most important predictive marker to EGFR-tyrosine kinase inhibitors (TKIs) and first-line gefitinib treatment will be approved in the near future for use in non-small cell lung cancer (NSCLC) patients with the EGFR mutation. Direct sequencing is known to be the standard for detecting EGFR mutations; however, it has limited sensitivity. Peptide nucleic acids (PNA)-mediated PCR clamping method is a newly introduced method for analyzing EGFR mutations with increased sensitivity and stability. Methods: A total of 71 NSCLC patients were analyzed for EGFR mutations using the PNA-mediated PCR clamping technique. Sixty-nine patients were analyzed for clinicopathologic correlation with EGFR genotype; 2 patients with indeterminate results were excluded. In order to determine EGFR-TKI drug response, 57 patients (42 gefitinib, 15 erlotinib) were included in the analysis. Results: The EGFR mutation rate was 47.8%. Being female, a non-smoker, and having adenocarcinoma were favorable clinicopathologic factors, as expected. However, more than a few smokers (33.3%), male (28.1%), and patients with non-adenocarcinoma (28.6%) had the EGFR mutation. Having a combination of favorable clinicopathologic factors did not increase the EGFR mutation rate significantly. Drug response to EGFR-TKIs showed significant differences depending on the EGFR genotype; ORR was 14.3% for wild type vs 69.0% for mutant type; DCR is 28.6% for wild type vs 96.6% for mutant type. The median EGFR-TKI treatment duration is 7.6 months for mutant type group and 1.4 months for wild type group. Conclusion: EGFR genotype determined using the PNA-mediated PCR clamping method is significantly correlated with the clinical EGFR-TKI responses and PNA-mediated PCR.

• Journal of Distribution Science
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• v.5 no.1
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• pp.57-73
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• 2007

There are more problems in distributing agricultural and special products than industrial products because they have such restrictions as seasonal fluctuation in the quantity, indeterminate and diverse shapes, many farm households, a small amount of shipment, and mandatory guide and maintenance. However, with social atmosphere called well-being, there are increasing concerns about inorganic agricultural products and direct trade with farmers. It is therefore urgent to stabilize the price of agricultural and special products and to correctly grasp and improve logistics activities for those dealing with agricultural and special products in order to supply fresh ones. This study aimed at examining logistics activities and the information technology level for agricultural and special products, determining how these factors affect logistics performance, and eventually suggesting a scheme to improve the logistics achievements for agricultural and special products. For this purpose, it selected information technology and logistics activity levels as independent variables and logistics performance as a dependent variable. The information technology level was measured for utility, holding level, and concern, respectively, and the logistics activity level was tested for the level of using the logistics information system in those companies concerned. From the above-mentioned findings, it is urgent to enhance distribution technology and its level immediately since distribution of agricultural and special products is poor in Korea and good distribution can contribute to a reduction of logistics costs or improvement of customer service.

• Current Research on Agriculture and Life Sciences
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• v.15
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• pp.7-16
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• 1997

The effects of liming and inoculation of nodule bacteria on the growth and yield of soybeans in the 1st and 3rd year paddy-upland rotated paddy fields was investigated. Compared with the 1st rotated field, the degree of nodulation was much higher in the 3rd rotated one with greater difference at flowering stage than at the early stage of soybean growth. At the same time, greater difference in the degree of nodulation was also recognized between soybean varieties. In the 3rd year rotated field, a great deal of nodulation effect was recognized in the no-limed field but not in the limed one. The growth of soybean was not much affected by liming and nodulation at early stage but it was much increased by liming at flowering stage, especially in Williams 79, indeterminate type variety. Most of yield components and yield of soybean at maturity was much increased by liming with greater in Williams 79 than in Namhaekong. Nodulation effect on the yield components and yield was great in the no-limed filed but not in the limed one for both varieties. The yield and yield components of soybean, without no relation with liming and nodulation, was much great in the 3rd year rotated paddy fields than in the 1st year one, which was inferred that the more rotated fields the more yield and better growth of soybeans could be possible in the paddy-upland rotatonal soybean croppings.

• Korean Journal of Plant Resources
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• v.33 no.1
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• pp.50-61
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• 2020

Pigeon pea (Cajanus cajan) is an important legume species that produces seeds that are rich in phenolic compounds and dietary nutrients. The aim of the present study was to evaluate the agricultural characters, phenolic and nutritional contents, and antioxidant capacities of seeds from 82 pigeon pea germplasms cultivated in the Republic of Korea. The accessions exhibited compact (24.39%), semi-spreading (74.39%), or spreading (1.22%) growth habits and determinate (89.02%), indeterminate (8.54%), or semi-determinate (2.44%) flowering patterns. Days to 75% maturity ranged from 30 to 72 d, and yield per plant ranged from 6.00 to 148.60 g. Meanwhile, total phenolic, crude protein, crude fiber, and dietary fiber contents ranged from 16.42 ± 0.62 to 29.67 ± 0.43 mg gallic acid equivalent per g of dried extract, from 16.76 ± 6.74% to 22.61 ± 0.05%, from 4.70 ± 0.24 to 8.63 ± 0.02%, and from 12.98 ± 0.71 to 33.19 ± 1.50%, respectively. In addition, DPPH radical-scavenging capacity ranged from 1.61 ± 0.10 to 16.04 ± 2.30 mg ascorbic acid equivalent per g of dried extract, and Trolox equivalent antioxidant capacity ranged from 3.03 ± 0.86 to 42.24 ± 0.72 mg Trolox equivalent per g of dried extract. Phenolic content was correlated with both DPPH radical-scavenging capacity (r = 0.63) and Trolox equivalent antioxidant capacity (r = 0.29). Nine accessions (IT170290, IT170291, IT170270, IT170276, IT170379, IT170386, IT170388, IT170418, and IT170340) exhibited early maturity, compact and erect growth habits, and above average antioxidant activities and phenolic and protein contents. In addition, accessions IT170290 and IT170291 were especially promising pigeon pea germplasms to grow, owing to various favorable characteristics (e.g., high yield and dietary fiber content). Hence, these accessions could be useful cultivars to the Republic of Korea if considered in future agricultural systems.

• Fisheries and Aquatic Sciences
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• v.1 no.1
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• pp.82-93
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• 1998

Monthly changes of the gonad follicle index (GFI), reproductive cycle, egg-diameter composition, first sexual maturity of the Pacific oyster, Crassostrea gigas, were studied based on the samples which have been collected from the intertidal zone of Poryong west coast of Korea, from January to December, 1996. C. gigas, is dioecious, while a few individuals are alternatively hermaphroditic. Monthly variation of gonad follicle index (GFI) used for determination of spawning period, coincided with the reproductive cycle. GFI increased from April when seawater temperatures gradually increased and reached the maximum in May. And then, GFI sharply decreased from June to September due to spawning. Reproductive cycle of this species can be divided into five successive stages: in females, early active stage (March to April), late active stage (April to May), ripe stage (May to August), partially spawned stage (June to September) and spent/inactive stage (September to February); in males, early active stage (February to March), late active stage (April to May), ripe stage (May to September), partially spawned stage (June to September) and spent/ inactive stage (September to February). The diameter of fully mature eggs are approximately 50um. Spawning occurred from June to September, and two spawning peaks were observed in June and August when the seawater temperature was above $20^{\circ}C$. Percentages of the first sexual maturity of males of 20.1-25.0 mm in shell height were over $50\%$, while those of females of 25.1-30.0 mm in shell height were over $50\%$. All the males of > 30.1 mm and all the females of ^gt; 35.1 mm completed their first sexual maturity. The results suggest that C. gigas has a protandry phenomenon. Sex ratios of 919 oysters observed were 453 females $(49.29\%)$, 429 males $(46.68\%)$, 16 hermaphrodites $(1.74\%)$, and 21 indeterminate individuals $(2.29\%)$. In age class I, sex ratio of males were $64.00\%$, thus, a higher percentage than that of females. It was noted that $64.00\%$ of the young males (age class I) were more functional than females in age class I, but 2-3 year-old oysters showed higher percentage of females. Percentages of hemaphrodites in 2-3 year classes were relatively higher than those in other year classes. Histological pattern of hermaphrodites can be divided into two types: Type I (hermaphrodite having a number of newly formed developing oocytes on the oogenic tissues within a degenerating spermatogenic follicle after discharge of numerous spermatozoa) and Type II (hermaphrodite having two separate follicles in the same gonad).

• Tuberculosis and Respiratory Diseases
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• v.76 no.1
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• pp.23-29
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• 2014

Background: Interferon-${\gamma}$ assays based on tuberculosis (TB)-specific antigens have been utilized for diagnosing and ruling out latent TB and active TB, but their utility is still limited for TB incidence countries. The aim of this study is to understand the clinical utility of enzyme-linked immunospot (ELISpot) assays among patients with clinically suspected TB and healthy adults in clinical practices and community-based settings. Methods: The ELISpot assays (T SPOT.TB, Oxford Immunotec, UK) were prospectively performed in 202 patients. After excluding those with indeterminate results, 196 were included for analysis: 41 were TB patients, 93 were non-TB patients, and 62 were healthy adults. Results: The sensitivity and negative predictive values of the T SPOT.TB assays for the diagnosis of TB were 87.8% and 89.1%, respectively, among patients with suspected TB. The agreement between the tuberculin skin test (10-mm cutoff) and the T SPOT.TB assay was 66.1% (kappa=0.335) in all participants and 80.0% (kappa=0.412) in TB patients. Among those without TB (n=155), a past history of TB and fibrotic TB scar on chest X-rays were significant factors that yielded positive T SPOT.TB results. There was a significant difference in the magnitude of T SPOT.TB spot counts between TB patients and non-TB patients or healthy adults. Conclusion: The T SPOT.TB assay appeared to be a useful test for the diagnostic exclusion of TB. A positive result, however, should be cautiously interpreted for potential positives among those without active TB in intermediate TB incidence areas.

• Proceedings of the Korea Concrete Institute Conference
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• 2008.11a
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• pp.309-312
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• 2008

Reinforced concrete (RC) structures have been most widely used because of the economic efficiency. However, it is very weak to tensile stresses and difficult to control deflection due to the heavy self-weight of concrete. Although it is generally known that prestressed concrete structures can be the most effective to overcome the demerit of RC structures, its application is very seldom in domestic construction for the difficult onsite circumstances. The post-tension method, which is well fit for buildings that are mostly indeterminate structures and beneficial for monolithic construction, has been introduced to just a few building construction. The application of full PT method into entire spans makes construction engineers feel very difficult due to the lack of current condition in construction fields. Therefore, this study proposed the partially applied PT method as an alternative, which can improve the deflection control of RC structures and reduce the construction difficulty by applying the PT method in a part of span length as needed, and analyzed its characteristics of structural behavior. In this study, the top anchorage was applied to improve the applicability of partial PT method, and the analysis results of slab behavior were compared to the measured values obtained from the post-tensioned slab constructed by the partial PT method.