• Biomolecules & Therapeutics
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• 제31권5호
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• pp.526-535
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• 2023

Breast cancer is the most common cancer and a frequent cause of cancer-related deaths among women wordlwide. As therapeutic strategies for breast cancer have limitations, novel chemotherapeutic reagents and treatment strategies are needed. In this study, we investigated the anti-cancer effect of synthetic homoisoflavane derivatives of cremastranone on breast cancer cells. Homoisoflavane derivatives, SH-17059 and SH-19021, reduced cell proliferation through G2/M cell cycle arrest and induced caspase-independent cell death. These compounds increased heme oxygenase-1 (HO-1) and 5-aminolevulinic acid synthase 1 (ALAS1), suggesting downregulation of heme. They also induced reactive oxygen species (ROS) generation and lipid peroxidation. Furthermore, they reduced expression of glutathione peroxidase 4 (GPX4). Therefore, we suggest that the SH-17059 and SH-19021 induced the caspase-independent cell death through the accumulation of iron from heme degradation, and the ferroptosis might be one of the potential candidates for caspase-independent cell death.

• 한국경영과학회지
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• 제26권1호
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• pp.71-85
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• 2001

This paper considers a cost model for a U-shaped manufacturing cell formation which incorporates a required number of machines and various material flows together under multi-part multi-cell environment. The model is required to satisfy both the specified operation sequence of each part and the total part demand volume, which are considered to derive material handling cost in U-shaped flow line cells. In the model several cost-incurring factors including set-up for batch change-over, processing time for operations of each part, and machine failures are also considered in association with processing load and capacity of each cell. Moreover, a heuristic for a good machine layout in each cell is newly proposed based on the material handling cost of each alternative sequence layout. These all are put together to present an efficient heuristic for the U-shaped independent-cell formation problem, numerical problems are solved to illustrate the algorithm.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2009년도 춘계학술대회 논문집
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• pp.331-334
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• 2009

본 연구에서는 우주 및 수중 동력원을 위한 연료전지 기반 공기 불요 추진 시스템을 개발하였다. 공기 불요 동력 시스템을 위해 과산화수소를 산화제로 선택하였고, 촉매 분해 반응을 통해 산소와 물을 발생하였다. 순수한 산소는 연료전지에 공급되고, 물은 분리한 후 저장된다. 본 연구에서는 고체 상태의 수소화붕소나트륨을 수소원으로 사용하였고, 촉매 가수분해 반응을 통해 순수한 수소를 발생할 수 있었다. 연료전지 기반 공기 불요 동력 시스템을 검증하기 위해 연료전지 시스템을 구축하였고 다양한 조건에서 평가를 수행하였다.

• 대한기계학회논문집A
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• 제29권12권
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• pp.1615-1620
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• 2005

We present a novel flow-rate independent cell counter using a fixed control volume between double electrical sensing zones. The previous device based on the single electrical cell sensing in a given flow-rate requires an accurate fluid volume measurement or precision flow rate control. The present cell counter, however, offers the flow-rate independent method for the cell concentration measurement with counting cells in a fixed control volume of $22.9{\pm}0.98{\mu}{\ell}$. In the experimental study, using the RBC (Red Blood Cell), we have compared the measured RBC concentrations from the fabricated devices with those from Hemacytometer. The previous and present devices show the maximum errors of $20.3\%\;and\;16.1\%$, which are in the measurement error range of Hemacytometer (about $20\%$). The present device also shows the flow-rate independent performance at the constant flow-rates ($5{\mu}{\ell}/min$ and $10{\mu}{\ell}/min$) and the varying flow-rate (4, 2, and $4{\mu}{\ell}/min$). Therefore, we demonstrate that the present cell counter is a simple and automated method for the cell concentration measurement without requiring an accurate fluid measurement and precision flow-rate control.

• BMB Reports
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• 제30권3호
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• pp.200-204
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• 1997

We eliminated introns from replication independent chicken H3.3 histone gene using a H3.3 cDNA clone and a genomic H3.3 clone. After introduction into Rat 3 cells, we observed its pattern of expression by analyzing mRNA from different phases of the cell cycle. Even without introns, the H3.3 gene was expressed constitutively at a low level throughout the cell cycle. This indicates that the introns in the H3.3 gene are not responsible for the cell cycle-independent expression of the gene. This result contradicts previous reports that suggested their importance in cell cycle regulated expression. We believe that other regions of the gene, promoter, coding region, and/or 3'-end of the gene, are involved in its expression pattern.

• 대한산업공학회지
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• 제23권3호
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• pp.581-595
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• 1997

The procedure of grouping the machines and parts to form cells is called manufacturing cell design. The manufacturing cell design is an important step in the development and implementation of advanced manufacturing systems. For the successful implementation of the manufacturing systems, identification of independent manufacturing cells, i.e., cells where parts are completely processed in the cell and no intercell movements, is necessary in the design phase. In this paper, we developed a mixed integer programming model and genetic algorithm based procedure to solve the independent manufacturing cells design problem considering the alternative process plans and machines duplication. Several manufacturing parameters such as, production volume, machine capacity, processing time, number of cells and cell size, are considered in the process. The model determines the process plan for parts, port families and machine cells simultaneously. The model has been verified with the numerical examples.

• Archives of Pharmacal Research
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• 제15권4호
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• pp.379-381
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• 1992

Phellinus linteus was examined on its immunostimulating activities using an in vitro imunization and plaque forming cell assay. When lymphocytes were exposed to the extract of Phellinus linteus, the number of antibody forming cell was increased. In in vitro plaque forming cell assay, the immunostimulating effect was about 4.8 and 5.0 times of unimmunized control in polyconal and T-independent antibody response, respectively. Especially, Phellinus linteus significantly increased the antigenicity of TNP-LPS used as T-independent antigen. But Phellinus linteus did now show a mitogenic effect on B-lymphcytes. These results suggest that immunostimulating activity of Phillinus lintues might be associated with a functional stimulation of B-lympohocyte involved in humoral immune response.

• Mycobiology
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• 제48권3호
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• pp.219-227
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• 2020

Androgen-independent prostate cancer accounts for mortality in the world. In this study, various extracts of a medical fungus dubbed Ganoderma formosanum were screened for inhibition of DU145 cells, an androgen-independent prostate cancer cell line. Results demonstrated that both hexane (GF-EH) and butanol (GF-EB) fraction of G. formosanum ethanol extract inhibited DU145 cell viability in a dose-dependent manner. GF-EH induced cell-cycle arrest in G1 phase of DU145 cells via downregulation of cyclin E2 protein expression. In addition, GF-EB triggered extrinsic apoptosis of DU145 cells by activating caspase 3 gene expression resulting in programed cell death. Above all, both GF-EH and GF-EB show lower toxicity to normal human fibroblast cell line compared to DU145 cell, implying that they possess specific drug action on cancer cells. This study provides a molecular basis of G. formosanum extract as a potential ingredient for treatment of androgen-independent prostate cancer.

• 한국콘텐츠학회:학술대회논문집
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• 한국콘텐츠학회 2003년도 춘계종합학술대회논문집
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• pp.351-355
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• 2003

포유류 동물의 시각피질 세포에 나타나는 특징은 크게 단순특징을 추출하는 simple cell과 복잡한 특징에 반응하는 complex cell로 구분된다. 이 연구에서는 입력영상에 독립성분분석을 적응하여 complex cell에 대응하는 복잡한 특징을 추출하였다. 이 결과는 시각피질 세포의 정보처리에 대한 방식을 이해하는 데 기여할 것이다.

• 한국콘텐츠학회논문지
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• 제3권2호
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• pp.19-23
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• 2003

포유류 동물의 시각피질 세포에 나타나는 특징은 크게 단순특징을 추출하는 simple cell과 복잡한 특징에 반응하는 complex cell로 구분된다. 특히, 하위 계층의 세포들은 단순특징을 추출하며, 상위 계층으로 갈수록 복합특징을 추출하는 세포들이 존재한다. 이 연구에서는 입력영상에 독립성분분석을 적용하여 complex cell에 대응하는 복잡한한 특징을 추출하였다. 이 결과는 시각피질 세포의 정보처리에 대한 방식에 대한 이해를 기반으로 시각정보처리 알고리즘을 개발하는 데 기여할 것이다.