• Biomolecules & Therapeutics
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• v.31 no.5
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• pp.526-535
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• 2023

Breast cancer is the most common cancer and a frequent cause of cancer-related deaths among women wordlwide. As therapeutic strategies for breast cancer have limitations, novel chemotherapeutic reagents and treatment strategies are needed. In this study, we investigated the anti-cancer effect of synthetic homoisoflavane derivatives of cremastranone on breast cancer cells. Homoisoflavane derivatives, SH-17059 and SH-19021, reduced cell proliferation through G2/M cell cycle arrest and induced caspase-independent cell death. These compounds increased heme oxygenase-1 (HO-1) and 5-aminolevulinic acid synthase 1 (ALAS1), suggesting downregulation of heme. They also induced reactive oxygen species (ROS) generation and lipid peroxidation. Furthermore, they reduced expression of glutathione peroxidase 4 (GPX4). Therefore, we suggest that the SH-17059 and SH-19021 induced the caspase-independent cell death through the accumulation of iron from heme degradation, and the ferroptosis might be one of the potential candidates for caspase-independent cell death.

• Journal of the Korean Operations Research and Management Science Society
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• v.26 no.1
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• pp.71-85
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• 2001

This paper considers a cost model for a U-shaped manufacturing cell formation which incorporates a required number of machines and various material flows together under multi-part multi-cell environment. The model is required to satisfy both the specified operation sequence of each part and the total part demand volume, which are considered to derive material handling cost in U-shaped flow line cells. In the model several cost-incurring factors including set-up for batch change-over, processing time for operations of each part, and machine failures are also considered in association with processing load and capacity of each cell. Moreover, a heuristic for a good machine layout in each cell is newly proposed based on the material handling cost of each alternative sequence layout. These all are put together to present an efficient heuristic for the U-shaped independent-cell formation problem, numerical problems are solved to illustrate the algorithm.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2009.05a
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• pp.331-334
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• 2009

An air-independent propulsion (AIP) system based on fuel cell technologies was developed for space and underwater applications in the present study. Hydrogen peroxide was selected as an oxidizer for space and underwater power applications where air independence is a must. Catalytic decomposition of hydrogen peroxide was used to generate oxygen and water. The pure oxygen was provided to a fuel cell and the water was stored separately. Sodium borohydride in the solid state was used as a hydrogen source in the present study. Pure hydrogen can be generated by a catalytic hydrolysis reaction. A fuel cell system was fabricated to validate the fuel cell based air-independent power system and was evaluated at the various conditions.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.29 no.12 s.243
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• pp.1615-1620
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• 2005

We present a novel flow-rate independent cell counter using a fixed control volume between double electrical sensing zones. The previous device based on the single electrical cell sensing in a given flow-rate requires an accurate fluid volume measurement or precision flow rate control. The present cell counter, however, offers the flow-rate independent method for the cell concentration measurement with counting cells in a fixed control volume of $22.9{\pm}0.98{\mu}{\ell}$. In the experimental study, using the RBC (Red Blood Cell), we have compared the measured RBC concentrations from the fabricated devices with those from Hemacytometer. The previous and present devices show the maximum errors of $20.3\%\;and\;16.1\%$, which are in the measurement error range of Hemacytometer (about $20\%$). The present device also shows the flow-rate independent performance at the constant flow-rates ($5{\mu}{\ell}/min$ and $10{\mu}{\ell}/min$) and the varying flow-rate (4, 2, and $4{\mu}{\ell}/min$). Therefore, we demonstrate that the present cell counter is a simple and automated method for the cell concentration measurement without requiring an accurate fluid measurement and precision flow-rate control.

• BMB Reports
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• v.30 no.3
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• pp.200-204
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• 1997

We eliminated introns from replication independent chicken H3.3 histone gene using a H3.3 cDNA clone and a genomic H3.3 clone. After introduction into Rat 3 cells, we observed its pattern of expression by analyzing mRNA from different phases of the cell cycle. Even without introns, the H3.3 gene was expressed constitutively at a low level throughout the cell cycle. This indicates that the introns in the H3.3 gene are not responsible for the cell cycle-independent expression of the gene. This result contradicts previous reports that suggested their importance in cell cycle regulated expression. We believe that other regions of the gene, promoter, coding region, and/or 3'-end of the gene, are involved in its expression pattern.

• Journal of Korean Institute of Industrial Engineers
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• v.23 no.3
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• pp.581-595
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• 1997

The procedure of grouping the machines and parts to form cells is called manufacturing cell design. The manufacturing cell design is an important step in the development and implementation of advanced manufacturing systems. For the successful implementation of the manufacturing systems, identification of independent manufacturing cells, i.e., cells where parts are completely processed in the cell and no intercell movements, is necessary in the design phase. In this paper, we developed a mixed integer programming model and genetic algorithm based procedure to solve the independent manufacturing cells design problem considering the alternative process plans and machines duplication. Several manufacturing parameters such as, production volume, machine capacity, processing time, number of cells and cell size, are considered in the process. The model determines the process plan for parts, port families and machine cells simultaneously. The model has been verified with the numerical examples.

• Archives of Pharmacal Research
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• v.15 no.4
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• pp.379-381
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• 1992

Phellinus linteus was examined on its immunostimulating activities using an in vitro imunization and plaque forming cell assay. When lymphocytes were exposed to the extract of Phellinus linteus, the number of antibody forming cell was increased. In in vitro plaque forming cell assay, the immunostimulating effect was about 4.8 and 5.0 times of unimmunized control in polyconal and T-independent antibody response, respectively. Especially, Phellinus linteus significantly increased the antigenicity of TNP-LPS used as T-independent antigen. But Phellinus linteus did now show a mitogenic effect on B-lymphcytes. These results suggest that immunostimulating activity of Phillinus lintues might be associated with a functional stimulation of B-lympohocyte involved in humoral immune response.

• Mycobiology
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• v.48 no.3
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• pp.219-227
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• 2020

Androgen-independent prostate cancer accounts for mortality in the world. In this study, various extracts of a medical fungus dubbed Ganoderma formosanum were screened for inhibition of DU145 cells, an androgen-independent prostate cancer cell line. Results demonstrated that both hexane (GF-EH) and butanol (GF-EB) fraction of G. formosanum ethanol extract inhibited DU145 cell viability in a dose-dependent manner. GF-EH induced cell-cycle arrest in G1 phase of DU145 cells via downregulation of cyclin E2 protein expression. In addition, GF-EB triggered extrinsic apoptosis of DU145 cells by activating caspase 3 gene expression resulting in programed cell death. Above all, both GF-EH and GF-EB show lower toxicity to normal human fibroblast cell line compared to DU145 cell, implying that they possess specific drug action on cancer cells. This study provides a molecular basis of G. formosanum extract as a potential ingredient for treatment of androgen-independent prostate cancer.

• Proceedings of the Korea Contents Association Conference
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• 2003.05a
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• pp.351-355
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• 2003

Neurons in the mammalian visual cortex can be classified into the two main categories of simple cells and complex cells based on their response properties. Here, we find the complex features corresponding to the response of complex cells by applying the unsupervised independent component analysis network to input images. This result will be helpful to elucidate the information processing mechanism of neurons in primary visual cortex.

• The Journal of the Korea Contents Association
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• v.3 no.2
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• pp.19-23
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• 2003

Neurons in the mammalian visual cortex can be dassified into the two main categories of simple cells and complex cells based on their response properties. Here, we find the complex features corresponding to the response of complex cells by applying the unsupervised independent component analysis network to input images. This result will be helpful to elucidate the information processing mechanism of neurons in primary visual cortex.