• Restorative Dentistry and Endodontics
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• v.21 no.2
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• pp.546-558
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• 1996

The purpose of this vitro study was to evaluate the activity of human pulpal cells to adhesive glycoprotein-coated and non-coated culture dishes. Well known adhesive glycoproteins were used, such as type I collagen, type IV collagen, fibronectin, laminin, and vitronectin. Each adhesive glycoproteins applied onto the culture dishes. In this study, the protein coated and non-coated dishes were classified as each groups. Human pulpal cells cultured onto each groups. After 24 hours, 48 hours, 72 hours incubation time, radioactivity with scintillation counter for evaluation of the activity of human pulpal cells. The results as follows : 1. After 24 hours incubation time, activity of human pulpal cells were best in laminin-coated group among groups. Then fibronectin, type I collagen group were better, and all proteins were better than control. 2. After 48 hours incubation time, activity of human pulpal cells were best in fibronectin coated group. 3. After 72 hours incubation time, activity of human pulpal cells were not significantly different in all of adhesive glycoproteins. 4. After 24 hours incubation time, activity of human pulpal cells were best in fibronectin and laminin coated group. Activity of human pulpal cells in type I collagen coated group were better after 24 hours incubation time then 48 hours incubation time.

• The Korean Journal of Nuclear Medicine
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• v.1 no.1
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• pp.37-54
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• 1967

The changes in histopathology of various organs and growth inhibition of the chick embryos incubated with radioactive sulfur ($^{35}S$) were experimentally studied. The various doses of $^{35}S$ were injected into the yolk sac at different intervals and the weight changes of the embryos were evaluated to determine the growth inhibition rates. The embryos sacrified on various incubation days were used for the study of histopathological changes in organs such as the bone, liver, kidney, gonad, and eye. Following were the results: 1) The weight changes of the $^{35}S$ treated groups were as follows: i. Embryos treated on the 5 th incubation day: No weight changes were noted on the 8th incubation day, however, the growth inhibition rate of 32.1% was noted in the group treated with $50{\mu}C$ and of 38.2% in the group treated with $150{\mu}C$ on the 12th incubation day. The rates were 9.1 and 12.1% on the 15th incubation day, and 6.5 and 10.6% on the 18th incubation day respectively. ii. Embryos treated on the 8th incubation day: The growth inhibition rates on the 12th, 15th and 18th incubation days in the groups treated with $50{\mu}C$ were 20.9, 25.9 and 18.8% and in those treated with $150{\mu}C$ were 20.0, 14.9 and 16.9% respectively. iii. Embryos treated on the 12th incubation day: The growth inhibition rates on the 15th and 18th in the groups treated with $50{\mu}C$ were 13.6 and 21.1% and in those treated with $150{\mu}C$ were 26.7 and 6.5% and in those treated with $250{\mu}C$ were 10.6 and 12.6% respectively. iv. Embryos treated on the 15th incubation day: The growth inhibition rates on the 18th in the groups treated with $50{\mu}C$ were 6.5% and in those treated with $150{\mu}C$ were 10.1% and in those treated with $250{\mu}C$ were 8.5% respectively. In summary, the longer the incubation days, the less the growth inhibition rates. II) The histopathological changes in the various organs were as follows: i. Bone: Hyperplasia and edematous changes of the bone cavity, irregular distribution of immature granular cells and increased number of the myeloblast, megakaryocyte and reticuloendothelial cells were noted. ii. Liver: The embryos treated with $150{\mu}C\;of\;^{35}S$ on the 8th incubation day showed necrosis and nucleolysis of the liver cell and abnormal enlargement of sinusoid on the 12th incubation day. The longer the incubation days, the more severe the changes such as the pyknotic artophy of the liver cells and heterochromatism. The embryos treated on the 5th incubation day with 50 and $150{\mu}C\;of\;^{35}S$ showed little changes, but sight enlargement and accumulation of serous fluid in the sinusoid on the 8th incubation day. iii. Kidney: No particular changes except atrophic changes of epithelium were noted in early stage, however, the infiltration of the granular cell and monocyte into the cortex and pyknotic changes of vascular glomeruli were noted in later stage. These changes were not closely related to the doses of $^{35}S$ given. iv. Gonad: The degenerative changes such as destruction of the immature germ cells, hyperplasia and vacuolization of the stroma were noted in testis and ovary. v. Eye: A slight distortion of the cornea and sclera was noted. The hypertrophy of inner layer and blood cell infiltration into the vascular layer of the choroid membrane were noted in embryo groups on the 12, 15 and 18th incubation days.

• Korean Journal of Environment and Ecology
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• v.28 no.2
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• pp.105-112
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• 2014

During incubation, pelagic seabirds undergo large variations in their body mass as a result of concomitant fasting. In Procellariifomes undergoing important changes in body mass through the incubation, body mass regulation could be an important factor in behavioural decisions for changing activities during the incubation. The aim of this study was to investigate the effect of individuals' quality and incubation behaviour on their body mass regulation in Streaked Shearwaters Calonectris leucomelas breeding at Sasudo Island, in Jeju, South Korea during incubation period, June to August in 2013. In our results, we found that there was no relationship between the daily body mass loss and body size or condition of Streaked Shearwaters. However, the daily body mass loss increased significantly as the incubation bout increased. Moreover, the daily incubation bout was positively related to their body size. Consequently, although breeders' qualities were not related to their daily body mass loss during the incubation, the incubation behaviour affecting their daily body mass loss was regulated by their body size. Therefore, the body size of Streaked Shearwater breeders is one of important factors in their decisions for the incubation behaviour.

• Journal of Mushroom
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• v.13 no.3
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• pp.223-227
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• 2015

The study was conducted to investigate the effects of post-incubation period and temperature treatment conditions during incubation on the uniform primordia formation and cultural characteristics of oyster mushroom (Pleurotus ostreatus). Three kinds post-incubation period; 25, 30, 35 days and control were applied for 30 days while two kinds incubation room temperature $23^{\circ}C$ and $26^{\circ}C$ and control were used $20^{\circ}C$. The substrate temperature during pre-incubation was of 'Suhan No. 1' and 'Gonji No. 7'. Oyster mushroom varieties tended to increase between $24^{\circ}C$ to $26^{\circ}C$ at 11 to 15 days after inoculation and then they were maintained in treatment temperature during post -incubation period. The $CO_2$ occurrence was at the highest at 6,500 ppm for 'Suhan No. 1' and 5,800 ppm for 'Gonji No. 7' at the time of the highest temperature increase. The ratio of un-uniformal primordia formation and the ratio of non-commercial fruit body were reduced by 40%, 10.5%, respectively compared to control for 'Suhan No. 1' when in the post-incubation temperature was $26^{\circ}C$, and incubated for 10 days and 15 days treatment. Also, 'Gonji No. 7' was reduced by 19%, 9.5%, respectively when in the post-incubation temperature was $26^{\circ}C$, and incubated for 10 days treatment. Therefore, the higher post-incubation temperature of room and longer post-incubation period resulted in the higher percentage of primordia formation of two cultivars.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.6 no.3
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• pp.19-42
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• 2011

This research focuses on the case studies for operating scheme of technology venture incubation centers located at science parks, technology parks, and foreign universities. Technology venture incubation centers in foreign countries commonly vitalize local economy, create job markets, and promote new venture industry. There is a distinct difference in many ways between foreign and domestic incubation centers. Foreign countries consider the position of incubation centers at national policy level. They characterize the operation of incubation centers into technology fields. They install the networks cooperating with experts and promote the management. Tenant companies moved in incubation centers enhance the degree of completion through industry-university cooperation. Universities and research centers utilize the results of R&D to make the business, accelerate to venture establishment with technical skills, and foster technology entrepreneurs by spin-offs. Through case studies we propose implications helpful to manage domestic technology incubation centers and management methods to be successful centers.

• Biomolecules & Therapeutics
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• v.20 no.1
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• pp.104-112
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• 2012

The fruit of Terminalia chebula Retzius has been used as a panacea in India and Southeast Asia but its biological activities have not been fully elucidated. Here we report anti-arthritic and analgesic effect of NDI10218, a standardized ethanol extract of Terminalia chebula, on collagen-induced arthritis and acetic acid-induced writhing model, respectively. Arthritis was induced in DBA/1J mice by immunizing bovine type II collagen and mice were treated with NDI10218 daily for 5 weeks after the onset of the disease. NDI10218 reduced the arthritis index and blocked the synovial hyperplasia in a dose-dependent manner. The serum levels of pro-inflammatory cytokines TNF-${\alpha}$, IL-6, and IL-$1{\beta}$ were significantly reduced in mice treated with NDI10218. Production of the inflammatory IL-17, but not immunosuppressive IL-10, was also inhibited in splenocytes isolated from NDI10218-treated arthritis mice. Administration of NDI10218 markedly decreased the number of T cell subpopulations in the regional lymph nodes of the arthritis mice. Finally, NDI10218 reduced the number of abdominal contractions in acetic acid-induced writhing model, suggesting an analgesic effect of this extract. Taken together, these results suggest that NDI10218 can be a new therapeutic candidate for the treatment of rheumatoid arthritis.

• Korean Journal of Food Science and Technology
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• v.17 no.6
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• pp.516-521
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• 1985

Changes in phosphorus compounds and phytic acid of mung bean, soybean, sesame and perilla were investigated during soaking in water, germination, incubation at $60^{\circ}C$ and autoclaving at $120^{\circ}C$. The results showed that total P in all the seeds was decreased slightly by soaking and incubation, but increased in mung bean, soybean during germination. Inorganic P was increased for all the seeds after soaking for 24 hrs, while its content in mung bean, soybean and sesame was significantly increased during germination. Phytic acid content in mung bean, soybean and sesame was significantly seduced by 92 %, 76 % and 78 %, respectively, after 5-6 days of germination. Soaking the seeds for 24 hrs resulted in the reduction of phytic acid by the range of 8-25 %. It was also found that incubation at $60^{\circ}C$ for 10 hrs or autoclaving at $120^{\circ}C$ for 4 hrs affected significantly for removal of phytic acid from the legume seeds.

• Clinical and Experimental Reproductive Medicine
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• v.26 no.3
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• pp.399-405
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• 1999

Objective: In the preparation of ICSI, cumulus and corona cells should be removed from the oocytes by using a combination of enzymatic (hyaluronidase) and mechanical (pipetting) methods. But little is known about the effects of different degrees of oocyte denudation and incubation time between denudation and sperm injection on the outcomes of ICSI. The aim of this study was to evaluate the effects of varying the degrees of oocyte denudation and the lengths of incubation time from denudation to sperm injection on the outcomes of ICSI. Methods: In experiment 1, patients (oocytes) were grouped into group A and B according to the degree of denudation, complete and partial, respectively. In experiment 2, patients (oocytes) were grouped into group I, II and III according to the length of incubation time of denuded oocytes until sperm injection as < 1, $1{\sim}2$ and >2 hours, respectively. Results: There was no significant difference between the degree of oocyte denudation on the survival, fertilization and development rates after ICSI procedure. In case of the incubation time of denuded oocytes until ICSI, survival rates was higher in group III (83.1 %) than in group I (61.5%, p<0.05) or group II (64.3%). However no statistically significant differences were found between incubation time and fertilization or development rates. Conclusions: This study reveals that the outcomes of ICSI are not affected by the degree (complete or partial) of oocyte denudation. However, the denuded oocytes with incubation period of more than 2 hours show better outcomes of ICSI than those with the incubation period of less than 2 hours.

• Clinical and Experimental Reproductive Medicine
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• v.49 no.4
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• pp.270-276
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• 2022

Objective: The present study assessed the biological characteristics of human spermatozoa at different time intervals (0, 1, 1.5, and 2 hours) after incubation at 37℃. Methods: Twenty-five normozoospermic semen samples were incubated at 37℃. Incubation was performed at four time intervals of 0 (after liquefaction), 1, 1.5, and 2 hours. The samples were evaluated for sperm parameters at each time interval. Results: The rate of sperm progressive motility decreased at 1.5 hours compared to 0 hours as well as 2 hours compared to 1 hour and 0 hours. The rate of non-motile spermatozoa also decreased after 2 hours compared to after 0 hours. No significant changes were observed in sperm viability (p=0.98) and non- progressive motility (p=0.48) at any time intervals. Abnormal sperm morphology increased at 1.5 hours of incubation time (p<0.001). No significant changes were observed in DNA fragmentation at 1 hour compared to 0 hours (median [interquartile range]: 19.5 [4] vs. 19 [4]), as well as at 1.5 hours compared to 1 hour (20 [5]). However, a significant increase in DNA fragmentation was observed at 1.5 hours compared to 0 hours. The mitochondrial membrane potential decreased remarkably after 1 hour of incubation time. No significant differences were observed in the acrosome reaction or malonaldehyde levels at any time point (p=0.34 and p=0.98, respectively). Conclusion: The incubation of normozoospermic samples before use in assisted reproductive technology should be less than 1.5 hours to minimize the destructive effects of prolonged incubation time on general and specific sperm parameters.

• World Technopolis Review
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• v.5 no.1
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• pp.61-70
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• 2016

Entrepreneurship development has increasingly become a global solution to address the problem of rising unemployment. Science, Technology and Innovation (STI) have become important tools in improving the economic performance and social well-being of nations. When universities and industry work together to push the boundaries of knowledge, they become a powerful engine for innovation and economic growth. This paper is based on focus group interviews and discussions conducted with key players involved in the HIT-Sandown-UNDP Business Incubation Programme in Harare Zimbabwe. The business incubation project sought to support young Zimbabweans to transform their technical prototypes into commercially and socially viable ventures. As a result, a total of 10 prototypes were refined and investor ready business plans were developed for capital sourcing purposes. It was only through the coming together of the partners that real transformation of the lives of the participants was achieved through learning valuable business skills, coaching and mentoring. University-industry partnerships are a useful vehicle of setting up sustainable business incubation centres.