• Proceedings of the Korean Reliability Society Conference
• /
• 2002.06a
• /
• pp.411-414
• /
• 2002

A class of block designs for general combining ability comparisons within two groups of inbred lines in diallel crosses is given. Use is made of balanced block designs obtained by cyclically developing a single initial block.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2006.04a
• /
• pp.128-129
• /
• 2006

• Proceedings of the Korean Society of Crop Science Conference
• /
• 1990.05a
• /
• pp.128-129
• /
• 1990

• Journal of Plant Biotechnology
• /
• v.4 no.3
• /
• pp.129-134
• /
• 2002

We report the development of a new selection system for the transformation of pepper plants by mannose. In order to achieve this, we first tested several factors related to regeneration conditions. Among the 30 inbred lines examined, line P9l5 was able to generate shoots at the highest rate from both cotyledons and hyporotyls in MS media. A dosage curve for optimizing the selection conditions was established by mixing mannose (range 0-50 g/L) and sucrose (range 0-30 g/L). The least selection pressure on shoot formation was created by a mixture of sucrose and mannose at 20 g/L and 15 g/L, respectively, and the threshold for ultimate tissue death was 50 g/L of mannose irrespective of the sucrose concentration. However, we found that mannose itself was not the sole inhibitor of pepper shoot development. High concentrations of sucrose (30 g/L) contributed additively to the inhibition of shoot formation at higher mannose concentrations. Genotype preference is a major factor that enhances regeneration ability in mannose media, as was observed in MS media. P9l5 and P410 line had high regeneration rates under mannose selection conditions in the presence of Agrobacterium infection. Different virulence levels of Agrobacterium strains did change the regeneration rates, probably due to interaction with the specificities of the inbred lines. Taken together, P9l5 offers the best pepper inbred line for transformation and we recommend a selection condition of 20 g/L of sucrose and 15 g/L or more of mannose up to 50 g/L in media.

• Korean Journal of Breeding Science
• /
• v.40 no.3
• /
• pp.291-294
• /
• 2008

Amylogram properties such as peak viscosity, hot viscosity, cool viscosity, breakdown, pasting consistency and setback were investigated by the Rapid Visco Analyzer and interpreted by the relationship among amylogram properties according to the varietal groups classified by the rapidity of grain filling (RGF) which was calculated by the percentage of grain weight at 15 days after heading to 40 days after heading. The 164 rice recombinant inbred lines from the cross of Milyang23 and Gihobyeo were used to get the basic information regarding the amylogram properties. The used recombinant inbred lines could be grouped into 4 varietal groups such as slow maturing (less than 40% of RGF), mid-slow maturing (41-60% of RGF),mid-fast maturing (61-80% of RGF), and fast maturing (more than 81% of RGF) groups based on the RGF. The peak viscosity and setback showed regular tendency according to the varietal groups classified by the RGF. Positive significant correlations were found between pasting consistency and setback, however negative significant correlations were found between breakdown and setback in all varietal groups.

• 한국균학회소식:학술대회논문집
• /
• 2015.05a
• /
• pp.42-42
• /
• 2015

Plasmodiophora brassicae, the causal agent of clubroot disease, does the most serious damage to the Brassica crops. The limited control approaches make that the identification of clubroot resistance (CR) is more important for developing CR cultivars of the Brassica crops. So far, 8 CR loci were mapped. However, the variation of P. brassicae leads to the rapid erosion of its resistance. To identify novel CR genes, we employed three mapping population, derived from crosses between Chinese cabbage and turnip inbred lines ($59-1{\times}ECD04$ and $BJN3-1{\times}Siloga$) or between Chinese cabbage inbred lines ($BJN3-1{\times}85-I-II$), to perform QTL analysis. Totally, 8 CR loci were indentified and showed race-specific resistance. Physical mapping of these 8 loci suggested that 4 were located previously mapped position, indicating they might be the same allele or different alleles of the same genes. Other 4 loci were found to be novel. Further, CR near isogenic line carrying each CR locus was developed based on the marker assisted selection. Verification of these CR loci was underway. Identification of these novel CR genes would facilitate to breed broad-spectrum and durable CR cultivars of B. rapa by pyramiding strategies.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.43 no.4
• /
• pp.199-204
• /
• 1998

This study was conducted to investigate the chromosomal locations and effects of quantitative trait loci (QTL) associated with chemical properties of rice (Oryza sativa L.). One hundred sixty four recombinant inbred lines (MGRILs) of $F_{11}$ were derived from the cross between Milyang 23, Tongil type, and Gihobyeo, japonica type. They were evaluated for 7 traits of chemical property in rice. Transgressive segregation was observed for all traits examined. Eight significant QTLs were detected (LOD$\geq$2.0) for five traits, including two QTLs for amylose content, two QTLs for potassium content, one QTL for ratio of magnesium to potassium, one QTL for fat content and two QTLs for ash content. Phenotypic variation explained by each QTL ranged from 7.2% to 14.4%. However, no significant QTL was detected for magnesium and protein contents. In amylose content and ash content M alleles originated from Milyang 23 were responsible for increasing these traits and J alleles originated from Gihobyeo also responsible for increasing these traits. Pleiotropic effects of single QTLs on different traits are observed.

• The Plant Pathology Journal
• /
• v.25 no.4
• /
• pp.422-428
• /
• 2009

Studies were conducted to identify the sources of resistance in mungbean recombinant inbred lines (RILs) in Thailand against mungbean yellow mosaic disease (MYMD). 146 mungbean RILs in $F_8$ series were evaluated in a field including resistant parent NM-10-12-1 and susceptible parent KPS 2 during summer 2008 under high inoculum pressure. The RILs were subsequently scored for disease symptom severity ratings (DSSR) using a new scale. Observations regarding DSSR and % disease index (%DI) showed that the tested RILs responded differently to the disease. A large number of RILs (132) were found highly susceptible, 12 were susceptible, 3 were tolerant and one was resistant. Overall screening results showed that three RILs, viz. line no. 30, 100 and 101 had minimum DSSR and % disease index thus they are good source of resistance to MYMD in spite of high disease pressure and can therefore be used directly as varieties to manage the disease in Thailand.

• Proceedings of the Korean Society of Plant Biotechnology Conference
• /
• 2004.10a
• /
• pp.62-65
• /
• 2004

To protect the watermelon against soil-borne pathogens, we are currently producing disease-resistant transgenic root stock for the growth of watermelon, A defensin gene (J1-1) from Capsicum annum, a ACC deaminase gene from Pseudomonas syringae, a galactinol synthase (CsGolS) gene from Cucumis sativus, and a WRKY (CvWRKY2) gene from Citullus vulgaris were used as transgenes for disease resistance. The gene were transformed into a inbred line (6-2-2) of watermelon, Kong-dae watermelon and a inbred line (GO702S) of gourd, respectively, by Agrobacterium-mediated transformation. Putative transgenic plants were selected in medium containing 100mg/L kanamycin, and then integration of the genes into the genomic DNA were demonstrated by PCR analysis. Successful integration of the gene in regenerated plants was also confirmed by PCR (Figf 1), genomic Southern blot (Fig 2), RT-PCR (Fig 3), and Northern blot analysis(Fig 4). Several T1 lines having different transgene were produced, and disease resistance of the T1 lines are under estimation.

• Korean Journal of Agricultural Science
• /
• v.19 no.2
• /
• pp.269-272
• /
• 1992

A new single glutinous maize hybrid "Huin Chal 1" was developed at the Genetics and Breeding Lab., Dept. of Agron., College of Agriculture, Chungnam National University. Parents for the hybrid were all derived from Korean indigenous maize lines which were collected in 1980s. One of the parental inbred lines was originated from Bosung, Chonnam province and other inbred line was from Jewon, Chungbuk province. Both inbreds were developed by ear-to-row selection method through selling and named as Bosung and Jewon, respectively. Performace tests for the hybrid which was produced between Bosung and Jewon have been conducted five times including one test at Crop Experiment Station, Rural Development Administration. From the previous performance tests, the hybrid was recognized as high yielding hybrid with good agronomic characteristics and we named the hybrid as Huin Chal 1., and hereby we report the agronomic characters of the newly developed hybrid.