• Title/Summary/Keyword: in vivo antifungal activity

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Effects of Compost Tea Making from Differently Treated Compost on Plant Disease Control

  • Tateda, Masafumi;Yoneda, Daisuke;Sato, Yukio
    • Journal of Wetlands Research
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    • v.9 no.1
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    • pp.91-98
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    • 2007
  • Antifungal activity of compost teas was evaluated in vitro and in vivo experiments. In vitro test, fourteen compost teas were produced using five different composts and through six different methods for compost tea production. Eleven pathogenic fungi were used as indicators of antifungal activity of compost teas. In vivo test, one of the compost teas used in vitro test was sprayed to cucumber leaves which were infected with powdery mildew in order to evaluate antifungal activity of compost tea. From the results of the tests, it was found that all compost teas used this study showed antifungal activity and the proper application of compost teas against pathogenic fungi for obtaining optimal effects was important to know.

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In Vitro and in Vivo Antifungal Activities of 6-[(N-4-bromophenyl)amino]-7-chloro-5,8-quinolinediones

  • Ryu, Chung-Kyu;Kim, Dong-Hyun;Kwon, Sang-Mee;Jung, Sung-Hee;Kim, Sung-Hee
    • Archives of Pharmacal Research
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    • v.20 no.6
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    • pp.586-589
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    • 1997
  • Antifungal activities of 6-[(N-4-bromophenyl)amino]-7-chloro-5,8-quinolinedione (RCK7) were tested. The MIC values of RCK7 were determined for antifungal suceptibility, in vitro against Aspergillus niger, Cryptococcus neoformans and Trichophyton mentagrophyte by standard agar streak method. In vitro, RCK7 showed more potent antifungal activity than fluconazole and ketoconazole. Also, RCK7 was tested for in vivo antifungal activity in the treatment of systemic infection with Candida albicans in normal mice. The therapeutic potential of RCK7 had been assessed by evaluating their survival rate against systemic infections compared with that of ketoconazole. $ED_{50}$ of intraperitoneally administered RCK7 ws $2.05{\pm}0.30mg/kg$ but that of ketoconazole was $8.00{\pm}0.73 mg/kg$, respectively. When RCK7 was administered intravenously at the $ED_{50}$(2.05 mg/kg). the colony counts of Candida albicans in the liver after 7 days and 14 days were reduced as likely as ketoconazole at the $ED_{50}(8.00 mg/kg)$, and the better survival rates than ketoconazole's were achieved after 14 days. The results suggest that RCK7 may be a potent antifungal agent.

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Isolation and in vivo Activities of Antifungal Compounds from Myxococcus sp. JW154 (Myxobacteria). (점액세균 Myxococcus sp. JW154로 부터 항균물질의 분리 및 식물병원균에 대한 in vivo 활성)

  • 안종웅;김병섭
    • Microbiology and Biotechnology Letters
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    • v.30 no.2
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    • pp.162-166
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    • 2002
  • Two bithiazole-type antibiotics were isolated from the culture broth of a Myxococcus species which isolated from the marine sediment off the coast of Cheju Island, Korea. The structures of these metabolites were determined as KR025 and melithiazole F, previously reported bithiazoles, using combined spectroscopic methods. Both compounds showed an antifungal activity. In in vivo tests, these compounds exhibited potent controlling activities against tomato late blight, wheat leaf rust, and barley powdery mildew with control values more than 80% at a concentraton of 20 $\mu\textrm{g}$/ml.

Antifungal activities of coumarins isolated from Angelica gigas and Angelica dahurica against Plant pathogenic fungi (당귀와 백지로부터 분리한 Coumarin계 물질들의 식물병원균에 대한 항균활성)

  • Ryu, Shi-Yong;Kim, Young-Sup;Kim, Heung-Tae;Kim, Seong-Ki;Choi, Gyung-Ja;Kim, Jeoung-Seob;Lee, Seon-Woo;Heor, Jung-Hee;Cho, Kwang-Yun;Kim, Jin-Cheol
    • The Korean Journal of Pesticide Science
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    • v.5 no.3
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    • pp.26-35
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    • 2001
  • In order to search potent antifungal substances from domestic plants, 40 plants cultivated in Korea were collected. After extracting with methanol (MeOH) and concentrating to dryness, the MeOH extracts were screened for in vivo antifungal activity against six plant diseases at a concentration of $2000{\mu}g/mL$. Fourteen extracts showed disease-controlling activity more than 90% against at least one of the 6 plant diseases tested; eight, seven, and three extracts controlled more than 90% the development of rice blast, tomato late blight, and wheat leaf rust, respectively. However, none of the extracts exhibited in vivo antifungal activity more than 90% against rice sheath blight, tomato gray mold, and barley powdery mildew. From the MeOH extracts of Angelica gigas and A. dahurica showing potent controlling activity against rice blast, 1 and 2 antifungal substances, respectively, were isolated by solvent partitioning and column chromatography. The three compounds were identified to be coumarins, namely, decursin, imperatorin, and isoimperatorin, by mass spectrometry and NMR spectroscopy. They were examined for in vitro and in vivo antifungal activities together with umbelliferone (7-bydroxycournarin) and scopoletin (6-methoxy-7-hydroxycoumarin) containing a free hydroxyl group at position 7 to investigate the structure-activity relationship. In vitro, most of 50% growth inhibitory concentrations ($IC_{50}$) were over $200{\mu}g/mL$, indicating that they have relatively weak antifungal activity. The antifungal activity of decursin and scopoletin, containing cyclic alkoxy groups instead of free hydroxyl group at position 7, was stronger than umbelliferone and scopoletin. Especially, decursin and imperatorin showed potent antifungal activities against Pythium ultimum and Magnaporthe grisea, respectively, with $IC_{50}$ values less than $25{\mu}g/mL$. In vivo, decursin and imperatorin showed potent antifungal activity against rice blast, whereas other coumarins hardly controlled the development of 6 plant diseases tested. These results suggest that the antifungal activity of 7-hydroxycoumarin derivative is substantially increased when the hydroxyl group at position 7 is protected by a stable cyclic alkoxy grouping.

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In Vivo Disease Control Efficacy of Isoquinoline Alkaloids Isolated from Corydalis ternata against Wheat Leaf Rust and Pepper Anthracnose

  • Han, Jae Woo;Shim, Sang Hee;Jang, Kyoung Soo;Choi, Yong Ho;Kim, Hun;Choi, Gyung Ja
    • Journal of Microbiology and Biotechnology
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    • v.28 no.2
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    • pp.262-266
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    • 2018
  • Phytochemicals have been considered as alternatives for synthetic fungicides because of their biodegradability and low toxicity. In this study, we found that the methanolic extract of Corydalis ternata suppressed the development of plant diseases caused by Puccinia triticina and Colletotrichum coccodes. As the antifungal substance, three isoquinoline alkaloids (dehydrocorydaline, stylopine, and corydaline) were isolated from C. ternata. These active compounds also exhibited in vivo antifungal activity against P. triticina and C. coccodes. Taken together, our results suggest that C. ternata and its active compounds can be used to control plant diseases.

In Vivo Antifungal Activities of 67 Plant Fruit Extracts Against Six Plant Pathogenic Fungi

  • Choi Gyung-Ja;Kim Jin-Cheol;Jang Kyoung-Soo;Lim He-Kyoung;Park Il-Kwon;Shin Sang-Chul;Cho Kwang-Yun
    • Journal of Microbiology and Biotechnology
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    • v.16 no.3
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    • pp.491-495
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    • 2006
  • Methanol extracts of fruits of 67 plants were screened for in vivo antifungal activity against Magnaporthe grisea, Corticium sasaki, Botrytis cinerea, Phytophthora infestans, Puccinia recondita, and Blumeria graminis f. sp. hordei. Among them, 13 plant extracts ($3,000\;{\mu}g/ml$) showed more than 90% disease-control efficacy against at least one of six plant diseases. Specifically, the extracts of Aleurites fordii, Angelica dahurica, Camellia japonica, Chamaecyparis pisifera, Pittosporum tobira, and Styrax japonica controlled more than 90% of the development of rice blast at $1,000{\mu}g/ml$. Extracts of both S. japonica and A. dahurica fruits at $333{\mu}g/ml$ concentration displayed strong antifungal activity against M. grisea on rice seedlings.

Synthesis and Phytopathogenic Activities of Isopropylphenyl Derivatives (Isopropylphenyl 유도체들의 합성과 식물병원균에 대한 항균활성)

  • Jang, Do-Yeon;Choi, Kyoung-Gil;Lee, Byung-Ho;Kim, Tae-Jun;Jung, Bong-Jin;Choi, Won-Sik
    • Applied Biological Chemistry
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    • v.50 no.3
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    • pp.178-186
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    • 2007
  • 42 compounds such as ester, sulfonyl ester, phosphoyl ester and ether derivatives of 4-isopropylphenol (I) and 2-isopropylphenol (II) were synthesized. These derivatives were identified by IR, GC/MS and $^{1}H-NMR$ spectra. Their in vitro antifungal activities were tested against 10 plant pathogenic fungi. Among them, several compounds showed potent in vitro antifungal activity. The selected compounds showing potent in vitro antifungal activity were tested for their in vivo antifungal acitvities against 5 plant diseases such as rice blast, rice sheath blast, cucumber anthracnose, cucumber gray mold and tomato late blight. As a result, 2-isopropylphenyl piperonyloate (II-7a) showed a potent in vivo antifungal activity against cucumber anthracnose and tomato late blight, 4-isopropylphenyl 4-methoxybenzenesulfonate (I-6b) effectively inhibited the development of rice blast.

Antifungal Activity of Streptomyces sp. Against Puccinia recondita Causing Wheat Leaf Rust

  • Yi, Yong-Sub;Kim, Seung-Hyun;Kim, Min-Woo;Choi, Gyung-Ja;Cho, Kwang-Yun;Song, Jae-Kyeong;Lim, Yoong-Ho
    • Journal of Microbiology and Biotechnology
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    • v.14 no.2
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    • pp.422-425
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    • 2004
  • To discover a potent strain against wheat leaf rust, soil samples collected from Ilgamho, Seoul, Korea were tested in vivo and a strain belonging to Streptomyces sp. was found to show good antifungal activity when fermented in a broth. The identification of the strain was carried out based on 16S rDNA analysis, and the active compound was separated from the fermented broth. Even though its structure was not determined completely, the authors report the results obtained so far indicate that the fermented broth of the strain showed activity against wheat leaf rust. Therefore, we propose that this may be a potential novel strain showing antifangal activity against Puccinia recondita.

Lead optimization of 2-imino-1,3-thiazolines and in vivo antifungal activity against rice blast (I) (2-이미노-1,3-티아졸린 유도체의 최적화 및 벼 도열병에 대한 방제활성 (I))

  • Hahn, Hoh-Gyu;Nam, Kee-Dal;Bae, Su-Yeal;Park, Ik-Kyu
    • The Korean Journal of Pesticide Science
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    • v.8 no.3
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    • pp.168-174
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    • 2004
  • In a course of the process for a lead optimization of 2-imino-l,3-thiazolines 1 which show a selective in vivo antifungal activity against rice blast, new compounds 2 in which C-5 was substituted by methyl group of the lead compound were synthesized and tested for the biological activity. Bromination of $\beta$-keto ester 7 followed by the reaction with thiourea and hydrolysis gave 2-imino-5-methyl-l,3-thiazoline carboxylic acid 3. Coupling reactions of 3 with aniline derivatives afforded 17 kinds of the corresponding 2-imino-5-methyl-l,3-thiazoline carboxanilides 2. Their in vivo antifungal activity against rice blast was weaker than that of 1, indicating that the in vivo antifungal activity of 2-imino-l,3-thiazolines was affected by the substituent at C-5. These results would be an important data for the molecular design in the lead optimization process of this series.

In vivo Antifungal Activity Against Various Plant Pathogenic Fungi of Curcuminoids Isolated from the Rhizomes of Curcuma longa

  • Cho, Jun-Young;Choi, Gyung-Ja;Lee, Seon-Woo;Lim, He-Kyoung;Jang, Kyung-Soo;Lim, Chi-Hwan;Cho, Kwang-Yun;Kim, Jin-Cheol
    • The Plant Pathology Journal
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    • v.22 no.1
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    • pp.94-96
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    • 2006
  • In a search for plant extracts with potent in vivo antifungal activity against various plant pathogenic fungi, the methanol extract of the Curcuma longa rhizomes effectively controlled the development of rice blast catised by Magnaporthe grisea and tomato late blight caused by Phytophthora infestans. Three curcuminoids such as curcumin, demethoxycurcumin, and bisdemethoxycurcumin were purified from the methanol extract of C. longa rhizomes as antifungal principles. Among the three curcuminoids, demethoxycurcumin was the most active to both rice blast and tomato late blight, followed in order by curcumin and bisdemethoxycurcumin. However, they all exhibited no or little in vivo antifungal activity against other fungal pathogens causing rice sheath blight (Corticium sasaki), tomato gray mold (Botrytis cinerea), wheat leaf rust (Puccinia recondita), or barley powdery mildew (Blumeria graminis f. sp. hordel).