• Title/Summary/Keyword: in vitro protein digestibility

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Metabolisable Energy, In situ Rumen Degradation and In vitro Fermentation Characteristics of Linted Cottonseed Hulls, Delinted Cottonseed Hulls and Cottonseed Linter Residue

  • Bo, Y.K.;Yang, H.J.;Wang, W.X.;Liu, H.;Wang, G.Q.;Yu, X.
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.2
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    • pp.240-247
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    • 2012
  • Dietary supplementation with conventional linted cottonseed hulls (LCSH) is a common practice in livestock production all over the world. However, supplementation with mechanically delinted cottonseed hulls (DCSH) and cottonseed linter residue (CLR) is uncommon. Cottonseed by-products, including LCSH, DCSH and CLR, were assessed by chemical analysis, an in situ nylon bag technique, an in vitro cumulative gas production technique and in vitro enzyme procedure. The crude protein (CP) content of CLR (302 g/kg dry matter (DM)) was approximately 3 times that of LCSH and 5 times that of DCSH. The crude fat content was approximately 3 times higher in CLR (269 g/kg DM) than in LCSH and 4 times higher than in DCSH. Neutral detergent fibre (311 g/kg DM) and acid detergent fibre (243 g/kg DM) contents of CLR were less than half those of DCSH or LCSH. Metabolisable energy, estimated by in vitro gas production and chemical analyses, ranked as follows: CLR (12.69 kJ/kg DM)>LCSH (7.32 kJ/kg DM)>DCSH (5.82 kJ/kg DM). The in situ degradation trial showed that the highest values of effective degradability of DM and CP were obtained for CLR (p<0.05). The in vitro disappearance of ruminal DM ranked as follows: CLR>LCSH>DCSH (p<0.05). The lowest digestibility was observed for DCSH with a two-step in vitro digestion procedure (p<0.05). The potential gas production in the batch cultures did not differ for any of the three cottonseed by-product feeds. The highest concentration of total volatile fatty acids was observed in CLR after a 72 h incubation (p<0.05). The molar portions of methane were similar between all three treatments, with an average gas production of 22% (molar). The CLR contained a higher level of CP than did LCSH and DCSH, and CLR fermentation produced more propionate. The DCSH and LCSH had more NDF and ADF, which fermented into greater amounts of acetate.

Changes in Quality of Seasoned and Smoked Squid During Processing (조미훈연 오징어의 가공중 품질변화)

  • RYU Hong-Soo;MUN Sook-Im;LEE Kang-Hoo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.25 no.5
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    • pp.406-412
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    • 1992
  • Changes in proximate composition and protein quality were determined to find out appropriate processing conditions of the seasoned and smoked squid(Neon flying squid, Ommastrephes bartrannii). Moisture and crude protein contents were severely reduced (p<0.05), while increasing of fat and ash contents were not apparent. Seasoning and smoking contributed iii enhancing TBA value. Trypsin inhibitor(Tl) content was not increased severely after those processing steps. TI content checked in the all steps of squid processing was not correlated with the TBA value of squid in the same processing step. An improved digestibility and protein efficiency ratio(PER) were observed in the all products except with steak(mechanically soften product) in vitro enzymatic digestibilities of both raw Neon flying squid meats(mantle and arm) were significantly inferior(p<0.05) to other squid species.

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Trials of the Utilization Method in Mixtures Swards II. Effects of grasing and cutting management on foragequality and soil hardness (혼파초지의 이용방법 비교시험 II. 방목 및 예취이용이 목초의 품질과 토양경도에 미치는 영향)

  • 신재순;이필상;박근제;윤익석
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.10 no.2
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    • pp.96-101
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    • 1990
  • This field experiment was undertaken to find out the effect of grazing and cutting management on the forage quality and soil hardness at different legumes sown swards of the experimental field of Livestock Experiment Station in Suweon, from August 1985 to October 1988. The results obtained are as follows: 1. Crude protein, crude fat and ash contents under grazing were higher than these under cutting, but showed the reverse results in crude fiber and nitrogen free extracts contents. Average in vitro dry matter digestibility (IVDMD) under grazing (72 %) was a little higher than that under cutting (71 %). On the other hand, there was little difference among the mixtures swards in mineral contents and IVDMD. 2. The contents of Ca, P and Mg were a little high in the cutting, but K and Na contents were in gazing. Ca: P ratio in grazing (2.97) was slightly higher than that of cutting (2.94). 3. Production of energies(TDN, StE and NEL) with cutting were appeard to increase by 19.4 %, 13.5 %and 19.0 % than those of grazing, respectively. Mixtures swards including alfalfa showed more production both grazing and cutting. 4. Soil hardness in the top lOcm of soil by cattle grazing was changed from 15.5mm at startihg year to 22.6 mm after 3 years, but in cutting slightly increase from 15.5mm to 16.2mm. Generally it was low in early spring and high in autumn.

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Characteristics of Tenebrio molitor larvae and Bombyx mori pupae sequentially fermented with Aspergillus oryzae and Bacillus subtilis (Aspergillus oryzae와 Bacillus subtilis를 순차접종 발효한 갈색거저리유충과 누에번데기 발효물의 품질특성)

  • Kang, Sanghun;Chung, Chang-Ho
    • Korean Journal of Food Science and Technology
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    • v.54 no.1
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    • pp.94-102
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    • 2022
  • Defatted soybean, larvae of brown mealworm (Tenebrio molitor), and powdered pupae of silkworm (Bombyx mori) were fermented in solid and liquid forms using Aspergillus oryzae and Bacillus subtilis. The protein degradation rate (NDR) through solid fermentation was the highest in the fermented soybean control sample (54.69±6.54%), followed by silkworm pupae (34.82±5.99%) and brown mealworm larvae (30.54±3.80%). When these edible insects were fermented in liquid form, solid extraction yield was 37.73-46.88%, and protein yield was 47.47-63.02%. NDR of fermented liquid form products increased to 58.90, 52.62, and 50.13% for soybean, brown mealworm larvae, and silkworm pupae, respectively. SDS-PAGE of the liquid fermented products confirmed that microbial fermentation decomposed higher-molecular-weight proteins into small polypeptides. In vitro digestibility of liquid forms of edible insects increased by 1.26 to 1.53 times after fermentation. The protein solubility, foaming ability, and foam stability of liquid-fermented edible insects all tended to increase through fermentation.

Addition of Novel Lactobacillus plantarum KCC-10 and KCC-19 to Improve Fermentation Quality and Characterization of Italian Ryegrass Silage

  • Srigopalram, Srisesharam;Ilavenil, Soundharrajan;Vijayakumar, Mayakrishnan;Park, Hyung Soo;Lee, Kyung Dong;Choi, Ki Choon
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.35 no.3
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    • pp.195-200
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    • 2015
  • An investigation was carried out to evaluate the potential role of Lactobacillus Plantarum KCC-10 and KCC-19 on the quality and fermentation characterization of Italian ryegrass (IRG) silages. The physiochemical properties of IRG silage such as crude protein content, neutral detergent fiber, acid detergent fiber, total digestible nutrient and in vitro dry matter digestibility were not affected by KCC-10 and KCC-19. The pH of IRG silage in KCC-10 and KCC-19 treatments decreased compared to the control (p<0.05), while the lactic acid content in KCC-10 and KCC-19 treatments increased compared to the control (p<0.05). In addition, the number of lactic acid bacteria (LAB) in the KCC-10 treatment increased compared to the control (p<0.05). The number of lactic acid bacteria in KCC-19 increased, but there was no significant difference in all treatments. Therefore, we recommend L. plantarum KCC-10 and KCC-19 as potential additive candidates in IRG silage with lots of advantages.

Effects of cultivar and harvest days after planting on dry matter yield and nutritive value of teff

  • Saylor, Benjamin A;Min, Doohong;Bradford, Barry J
    • Journal of Animal Science and Technology
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    • v.63 no.3
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    • pp.510-519
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    • 2021
  • One of the most pressing issues facing the dairy industry is drought. In areas where annual precipitation is low, irrigation for growing feed presents the greatest water-utilization challenge for dairy producers. Here, we investigated the effects of cultivar and harvest days after planting (DAP) on dry matter (DM) yield and nutritive value of teff (Eragrostis tef), a warm-season annual grass native to Ethiopia that is well adapted to drought conditions. Eighty pots were blocked by location in a greenhouse and randomly assigned to four teff cultivars (Tiffany, Moxie, Corvallis, and Dessie) and to five harvest times (40, 45, 50, 55, or 60 DAP). Cultivars had no effect on DM yield and nutritive value. As harvest time increased from 40 to 60 DAP, DM yield and ash-free neutral detergent fiber (aNDFom) concentrations increased, while crude protein (CP) concentrations and in vitro NDF digestibility decreased. To assess carryover effects of time of harvest on yield and nutritive value, two additional cuttings were taken from each pot. Increasing first-cutting harvest time decreased CP concentrations in the second cutting and reduced DM yields in the second and third cutting. Harvesting teff between 45 and 50 DAP best optimized forage yield and nutritive value in the first and subsequent cuttings.

Effects of Nitrogen Fertilization Levels and Some Additives on the Chemical Composition and Silage Quality of Forage Rape (Brassica napus Subsp. oleifera) I. Effects of harvestinh dates and N forage rape (질소시비수준과 첨가제가 사초용 유채의 사료가치 및 사일리지의 품질에 미치는 영향 I. 예취시기 및 질소시비수준이 수량 및 사료가치에 미치는 영향)

  • 조무환;김동암
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.8 no.1
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    • pp.33-39
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    • 1988
  • Insufficient herbage during the fall in the temperate areas of the world has been a serious limitation in animal production, but leafy brassicae are potentially very useful for extending the grazing season when the growth and quality of grass is poor. This study was conducted to determine the effects of cutting stage and N fertilization on dry matter (DM) yield, forage quality and chemical composition of forage rape (Brassica napus Subsp. oleifera). The experiment was conducted in the Experimental Livestock Farm of Seoul Nat'l Univ., at Suweon, during 1985- 1986. The results obtained are summarized as follows: 1. DM percentage and yield significantly increased as the growth period was prolonged and decreased as the N fertilization increased, but in-vitro dry matter digestibility (IVDMD) was unaffected by the treatments. 2. Plant height, DM yield and $NO_3$-N content significantly increased as the rate of N fertilization increased and DM percentage decreased, but the IVDMD was unaffected. 3. The forage rape had high percentage of crude protein and ash. Glutamic acid, pro!ine and aspartic acid were the major amino acids. Concentration of P, Ca and Mg were high.

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Evaluation of the nutritional value of locally produced forage in Korea using chemical analysis and in vitro ruminal fermentation

  • Ki, Kwang Seok;Park, Su Bum;Lim, Dong Hyun;Seo, Seongwon
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.3
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    • pp.355-362
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    • 2017
  • Objective: The use of locally produced forage (LPF) in cattle production has economic and environmental advantages over imported forage. The objective of this study was to characterize the nutritional value of LPF commonly used in Korea. Differences in ruminal fermentation characteristics were also examined for the LPF species commonly produced from two major production regions: Chungcheong and Jeolla. Methods: Ten LPF (five from each of the two regions) and six of the most widely used imported forages originating from North America were obtained at least three times throughout a year. Each forage species was pooled and analyzed for nutrient content using detailed chemical analysis. Ruminal fermentation characteristics were also determined by in vitro anaerobic incubations using strained rumen fluid for 0, 3, 6, 12, 24, and 48 h. At each incubation time, total gas, pH, ammonia, volatile fatty acid (VFA) concentrations, and neutral detergent fiber digestibility were measured. By fitting an exponential model, gas production kinetics were obtained. Results: Significant differences were found in the non-fiber carbohydrate (NFC) content among the forage species and the regions (p<0.01). No nutrient, other than NFC, showed significant differences among the regions. Crude protein, NFC, and acid detergent lignin significantly differed by forage species. The amount of acid detergent insoluble protein tended to differ among the forages. The forages produced in Chungcheong had a higher amount of NFC than that in Jeolla (p<0.05). There were differences in ruminal fermentation of LPF between the two regions and interactions between regions and forage species were also significant (p<0.05). The pH following a 48-h ruminal fermentation was lower in the forages from Chungcheong than from Jeolla (p<0.01), and total VFA concentration was higher in Chungcheong than in Jeolla (p = 0.05). This implies that fermentation was more active with the forages from Chungcheong than from Jeolla. Analysis of gas production profiles showed the rate of fermentation differed among forage species (p<0.05). Conclusion: The results of the present study showed that the nutritional values of some LPF (i.e., corn silage and Italian ryegrass) are comparable to those of imported forages widely used in Korea. This study also indicated that the nutritional value of LPF differs by origin, as well as by forage species. Detailed analyses of nutrient composition and digestion kinetics of LPF should be routinely employed to evaluate the correct nutritional value of LPF and to increase their use in the field.

Broken rice in a fermented total mixed ration improves carcass and marbling quality in fattened beef cattle

  • Kotupan, Salisa;Sommart, Kritapon
    • Animal Bioscience
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    • v.34 no.8
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    • pp.1331-1341
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    • 2021
  • Objective: This study aimed to determine the effects of replacing cassava chips with broken rice in a fermented total mixed ration diet on silage quality, feed intake, ruminal fermentation, growth performance, and carcass characteristics in the final phase of fattening beef cattle. Methods: Eighteen Charolais-Thai native crossbred steers (average initial body weight: 609.4±46 kg; average age 31.6 mo) were subjected to three ad libitum dietary regimes and were maintained in individual pens for 90 d before slaughter. The experimental design was a randomized complete block design by initial age and body weight with six replicates. The dietary regimens used different proportions of broken rice (0%, 16%, and 32% [w/w] of dry matter [DM]) instead of cassava chips in a fermented total mixed ration. All dietary treatments were evaluated for in vitro gas production and tested in in vivo feeding trials. Results: The in vitro experiments indicated that organic matter from broken rice was significantly more digestible than that from a cassava-based diet (p<0.05). Silage quality, nutrient intake, ruminal fermentation characteristics, carcass fat thickness, and marbling score substantially differed among treatments. The ruminal total volatile fatty acids, propionate concentration, dietary protein intake, and digestibility increased linearly (p<0.05) with broken rice, whereas acetate concentration and the acetate:propionate ratio decreased linearly (p<0.05) with broken rice (added up to 32 g/kg DM). Broken rice did not influence plasma metabolite levels or growth performance (p>0.05). However, the marbling score increased, and the carcass characteristics improved with broken rice. Conclusion: Substitution of cassava chips with broken rice in beef cattle diets may improve fattened beef carcass quality because broken rice increases rumen fermentation, fatty acid biosynthesis, and metabolic energy supply.

Comparative nitrogen use efficiency of urea and pig slurry for regrowth yield and nutritive value in perennial ryegrass sward

  • Park, Sang Hyun;Lee, Bok Rye;Cho, Won Mo;Kim, Tae Hwan
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.4
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    • pp.514-522
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    • 2017
  • Objective: The study aimed to assess the N use efficiency (NUE) of pig slurry (in comparison with chemical fertilizer) for each regrowth yield and annual herbage production and their nutritive value. Methods: Consecutive field experiments were separately performed using a single application with a full dose of N (200 kg N/ha) in 2014 and by four split applications in 2015 in different sites. The experiment consisted of three treatments: i) control plots that received no additional N, ii) chemical fertilizer-N as urea, and iii) pig-slurry-N with five replicates. Results: The effect of N fertilization on herbage yield, N recovery in herbage, residual inorganic N in soil, and crude protein were significantly positive. When comparing the NUE between the two N sources (urea and pig slurry), pig slurry was significantly less effective for the earlier two regrowth periods, as shown by lower regrowth dry matter (DM) yield, N amount recovered in herbage, and inorganic N availability in soil at the 1st and 2nd cut compared to those of urea-applied plots. However, the effect of split application of the two N sources was significantly positive at the last two regrowth periods (at the 3rd and 4th cut). The two N sources and/or split application had little or no influence on neutral detergent fiber (NDF) content, acid detergent fiber (ADF) content, and in vitro DM digestibility, whereas cutting date was a large source of variation for these variables, resulting in a significant increase in in vitro DM digestibility for the last two regrowth periods when an increase in NDF and ADF content occurred. Split application of N reduced the N loss via nitrate leaching by 36% on average for the two N sources compared to a single application. Conclusion: The pig slurry-N was utilized as efficiently as urea-N for annual herbage yield, with a significant increase in NUE especially for the latter regrowth periods.