• 한국임상수의학회지
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• 제8권1호
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• pp.103-108
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• 1991

It was carried out to investigate the effect of the kind of medium, the concentration of serum added and the temperature of storage on the survival of mouse and bovine embryos preserved In vitro. The survival of frozen-thawed mouse embryos after cooling at 4$^{\circ}C$ was also investigated. It was possible to preserve the embryos of mouse until 4 days and of cattle in a day without significant decrease of the survival rates. The survival rates of frozen-thawed mouse embryos after cooling at 4$^{\circ}C$ over 3 days were below 20%.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2004년도 춘계학술발표대회
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• pp.256-256
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• 2004

The study was carried out to investigate the effects of preservation of ovaries and oocytes with and without cumulus cells and incubation time on zona penetration by canine spermatozoa. The objective of this study was to produce in vitro fertilized oocytes and solute canine sterile. (omitted)

• 한국식품저장유통학회지
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• 제30권3호
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• pp.547-548
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• 2023

• Asian-Australasian Journal of Animal Sciences
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• 제19권11호
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• pp.1617-1622
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• 2006

Long-term storage of feeds or feedstuffs in high temperature and humid conditions can be difficult because of microbial contamination. Essential oil isolated from industrial waste citrus peel could be used as a preservative because it is likely to have anti-bacterial and anti-fungal activity. Our objective was to determine whether different levels (0.028, 0.056 and 0.112 g/kg) of citrus essential oil (CEO) would provide anti-microbial activity and enhance preservation of animal feed without influencing rumen fermentation. At 0.112 g/kg, CEO inhibited growth of Escherichia coli (ATCC 25922) and Salmonela enteritidis (IFO 3313). Growth of E. coli recovered after 24 h of incubation, but S. enteritidis continued to be inhibited for 72 h. Preservation of antibiotic-free diets for swine was assessed by observing anti-aflatoxin activity. Aflatoxin was detected in control feed samples on days 16 (8 ppb) and 21 (8 ppb) and in anti-fungal agent (AA) treated samples on days 16 (2 ppb) and 21 (4 ppb). However, aflatoxin was not detected in feed samples treated with CEO. Treatment with CEO and AA did not influence ruminal pH, dry matter digestibility (DMD) or organic matter digestibility (OMD) over 48 h of incubation in rumen fluid. Acetate and propionate were slightly higher with CEO treatment (p<0.05), but total concentration of volatile fatty acid (VFA) was not significantly affected by treatment. Ammonia-N concentration was slightly higher for the control treatment (p<0.05). This study showed that treating feed with CEO enhances preservation of animal feed without influencing in vitro rumen fermentation.

• 한국식품저장유통학회지
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• 제30권3호
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• pp.538-545
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• 2023

본 연구는 probiotics mixture(Lactobacillus acidophilus PBS066(DSM 24936), Lactiplantibacillus plantarum PBS067(DSM 24937), Limosilactobacillus reuteri PBS072(DSM 25175))의 안전성 및 탄수화물 소화 효소 저해 효과를 in vitro로 평가하는 것을 목적으로 한다. 모든 균주는 유럽식품안전처(EFSA) 지침에서 권장하는 항생제 내성 프로필을 충족시켰으며, 모든 균주는 용혈 활성 및 세포 독성을 나타내지 않았다. Probiotics mixture, L. plantarum PBS067 그리고 L. reuteri PBS072는 α-amylase 활성을 억제하는 것으로 나타났으며, probiotics mixture와 이의 균주 3종 모두 α-glucosidase 활성을 억제하는 것으로 나타났다. 이로써 본 연구에 사용한 probiotics mixture 및 이의 단일 균주 3종의 안전성과 탄수화물 소화 억제 효과를 확인하였으며, 따라서 probiotics mixture 이의 단일 균주 3종은 섭취하여도 안전하며 섭취 시 체내 탄수화물 대사를 잠재적으로 조절하는 것을 확인하였다.

• 한국수정란이식학회지
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• 제18권2호
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• pp.109-113
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• 2003

본 연구는 고양이의 불임 해결을 위한 방안의 하나로써 체외수정란을 생산할 목적으로 난자의 형태, 보존 및 번식주기별 난자의 체외발생에 미치는 영향을 조사하였다. 1. 신선 난소로부터 회수한 난구세포부착 및 나화 난자를 각각 배양했을 때 GV 및 MI으로의 체외성숙율은 74.3%와 25.7%와 및 28.6%와 11.4%,로써 난구세포 부착 난자가 나화 난자의 비해 높은 체외성숙율을 나타냈다. 2. 휴지기, 난포기 및 황체기 단계로 구분하여 채취한 난구세포 부착 난포란을 배양하였을 때 GV 및 MI기로의 체외성숙율은 각각 88.6%와 6.5%, 60.0%와 11.4%, 77.1%와 5.7%였다. 3. 신선 및 salt에 보존한 난소로부터 회수한 난구세포부착 및 나화 난자를 각각 배양했을 때 GV 및 MI으로의 체외성숙율은 74.3%와 25.7%, 37.1%와 11.4% 및 57.1%와 13.3%, 17.1%와 3.3%으로서 난구세포 부착 신선난자가 나화 또는 salt 보존 난자에 비해 높은 체외 성숙율을 나타냈다.

• Clinical and Experimental Reproductive Medicine
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• 제39권1호
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• pp.10-14
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• 2012

The ovarian follicles develop initially from primordial follicles. The majority of ovarian primordial follicles are maintained quiescently as a reserve for the reproductive life span. Only a few of them are activated and develop to an advanced follicular stage. The maintenance of dormancy and activation of primordial follicles are controlled by coordinated actions of a suppressor/activator with close communications with somatic cells and intra-oocyte signaling pathways. Many growth factors and signaling pathways have been identified and the transforming growth factor-beta superfamily plays important roles in early folliculogenesis. However, the mechanism of maintaining the dormancy and survival of primordial follicles has remained unknown for decades. Recently, since the first finding that all primordial follicles are activated prematurely in mice deficient forkhead box O3a, phosphatidylinositol 3 kinase/phosphatase and tensin homolog (PTEN) signaling pathway was reported to be important in the regulation of dormancy and initial follicular activation. With these informations on early folliculogenesis, clinical application can be expected such as in vitro maturation of immature oocytes or in vitro activation of follicles by PTEN inhibitor in cryopreserved ovarian cortical tissues for fertility preservation.

• Clinical and Experimental Reproductive Medicine
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• 제41권2호
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• pp.41-46
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• 2014

IVM refers to the maturation of immature oocytes in culture after their recovery from small antral follicles at the stage prior to selection and dominance. IVM requires little or no FSH in vivo and has been proposed as an alternative to conventional IVF, since it reduces the primary adverse effects caused by controlled ovarian stimulation, including the ovarian hyperstimulation syndrome. Moreover, IVM is a promising option for cases for which no standard protocol is suitable, such as FSH resistance, contraindications for ovarian stimulatory drugs, and the need for urgent fertility preservation. Recently, IVM has been used in women with regular cycles and normal ovaries. However, the pregnancy rate following IVM is suboptimal compared with that of conventional IVF, indicating that further studies to optimize the protocol and the culture conditions are warranted.

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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• pp.134-134
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• 2003

Bovine embryos produced by in vitro maturation, feretilization and development was examined for presevation and transfer. The fertilization medium used BO medium with 5 mM/$m\ell$ caffeine and 10$\mu\textrm{g}$/$m\ell$ heparin and adjusted to a pH of 7.2 to 7.4. The final concentration of spermatozoa was adjusted to 1$\times$$10^{6}$ cells/$m\ell$ motile sperm during fertilization in vitro. At 8~10 hrs after insemination, the oocytes were transferred into CR1aa medium and cultured for 7 days. Embryos were preserved by vitrification method for transfer. When the embryos of early, blastocyst and expanded blastocyst stages were frozen-thawed, the proportions of embryos with normal morphology 83.6, 88.1 and 85.2%. (중략)

• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2003년도 제3회 발생공학 국제심포지움 및 학술대회
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• pp.134-134
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• 2003