• 생물환경조절학회지
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• 제22권2호
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• pp.116-122
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• 2013

본 연구는 '후지' 품종에 대한 수분수로서 이용가능성을 확인하기 위하여 꽃사과 11품종의 특성 및 자가불화합성 유전자형을 분석하였다. 꽃사과 품종의 개화기는 '후지'에 비하여 개화시기가 2~7일 정도 빨랐고 화분발아율도 'Virginia'(74.4%) 외에 다른 꽃사과 품종들은 85.6~98.0%로 높은 결과를 보였다. '후지' 품종에 꽃사과 화분을 인공수분시킨 결과 착과율이 자연방임의 65.4%에 비해 'Adam's'는 76.8%로 11.4% 높았고, 나머지 품종들은 86~100%로 20.4~34.4% 높은 결과를 보였다. 꽃사과 화분으로 결실된 '후지' 품종의 종자수는 자연방임으로 결실된 과실에 비하여 13.8~42.3% 향상되었고, 과중은 346.7~376.8g으로 7.4~16.7% 정도 향상되었다. 과형지수는 무처리구 과실은 0.82로 비정형과 기준이었는데 비해 'Manchurian', 'Hopa A', 'Virginia', 'Prunifolia', 'Spectabilis' 등을 인공수분한 경우 0.87~0.88로 정형과 기준에 도달하였다. PCR분석을 통한 꽃사과 품종의 자가불화합성 유전자형을 확인한 결과 'Manchurian', 'Virginia', 'Sentinel', 'Adam's', 'Asiatica', 'Yantaishagou', 'Hanyaehanakaidou'에서 $S_3$, $S_5$, $S_9$, $S_{10}$, $S_{20}$, $S_{26}$이 확인되었고, 염기서열 분석을 통해 $S_5$('Manchurian', 'Virginia')와 $S_9$('Yantaishagou')가 보고된 자가불화합성 유전자형과 일치하였다.

• Journal of Plant Biotechnology
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• 제3권2호
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• pp.89-94
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• 2001

In vitro plant regeneration of inbred breeding line of hot pepper (Capsicum annuum L.) was established using leaf and petiole segments as explants. About 28 days old plants were excised and cultured on MS medium supplemented with TDZ and NAA or in combination with Zeatin. In all of the media compositions tested, combination of TDZ 0.5 mg/L, Zeatin 0.5 mg/L, and NAA 0.1 mg/L was found to be the best medium for shoot bud initiation. Young petiole was the most appropriate explant type for the plant regeneration as well as genetic transformation in hot pepper. In this study, HpMADS1 gene isolated from hot pepper was introduced using Agrobacterium-mediated transformation system. Based on the analysis of Southern blot and RT-PCR, HpMADS1 gene was integrated in the hot pepper genome. It has been known that floral organ development is controlled by a group of regulatory factors containing the MADS domain. Morphological characteristics in these transgenic plants, especially flowering habit, however, were not significantly altered, indicating this MADS gene, HpMADS1 may be non-functional in this case.

• 한국약용작물학회지
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• 제26권2호
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• pp.119-126
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• 2018

Background: The flowering plant Hippophae rhamnoides L. has been used for many studies on fruit or leaf extracts. This study was conducted to investigate the development of a new cosmetic material from H. rhamnoides fruits and leaves that have by antioxidant, anti-inflammatory and wrinkle improvement activities. Methods and Results: The antioxidant abilities of H. rhamnoides extracts, including of a water-soluble fruit powder (FW), a fatsoluble fruit powder (FF), a supercritical extract of fruit by-product (BS), and a mixture of leaf and fruit (MIX), were investigated in vitro. A DPPH radical assay for antioxidant activity was performed for these fractions alongside assay to evaluate the total phenolic and flavonoid content (TPC and TFC). As expected, the MIX had the highest DPPH radical scavenging activity ($RC_{50}=10.27{\mu}g/m{\ell}$), and the TPC and TFC also were highest in MIX ($225.7mg{\cdot}GAE/g$, and $25.18mg{\cdot}QE/g$, respectively). Nitric oxide (NO) production in LPS-induced RAW264.7 cells was estimated and the results indicated an over 75% decrease of NO production in FF and MIX. In other assays, the highest elastase inhibitory activity was found in FW. Conclusions: These results revealed that H. rhamnoides extracts have a high potential for antioxidant, anti-inflammatory and antiwrinkle activities. H. rhamnoides products are suggested to be applied as the functional materials of cosmetic ingredients.

• 한국초지조사료학회지
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• 제12권3호
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• pp.173-177
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• 1992

This experiment was carried out to determine the effect of harvesting dates on the dry matter yield and quality of "Kool grazer" winter rye(Seca1e cereale L.) as a soilage crop at the Forage Experimental Field of College of Agrlc. and Life Sciences, SNU, Suweon. Four-day harvasting interval was allocated to measure the growth, yield and quality of rye from April 14 to May 18, 1987. Rye was rapidly grown as 3 cm/day and the growth was linearly recorded from the elongation to flowering stage. The dry matter and in vitro digestible dry matter(1VDDM) yields per ha were linearly increased up to 0.26 and 0.19 tonlday from April 14 to May 4 and April 14 to 29, respectively, but major breaks in yield increases occurred at the 50% heading stage, than yields increased. The highest crude protein yield was observed at the first heading stage of April 29, then decreased. Acid detergent fiber (ADF) and crude protein(CP) contents of rye at the heading stage of May 5 were 35 and 17%, respectively and the nutritive value of rye in terms of ADF and CP contents was evaluated as the I st grade hay, but neutral detergent fiber(NDF) content of rye was 47 % at the boot stage ofApril 22 and evaluated as the 2nd grade hay in terms of NDF content. The results indicate that the first heading stage or the end of April would be the most suitable harvesting time for early maturing winter rye as a soilage crop in terms of yield and quality in the middle northwestern coast rcgion of Korea. of Korea.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.23-23
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• 2019

Cypripedium is an orchid genus of over 50 species widely distributed in the northern temperate zone, with a few taxa extending central America and the Himalayas and the southwesternmost tip of China in Asia. Of the known species approximately two thirds are found in China. In Korea, 4 species were reported to find with rare popularity ; C. macranthos, C. guttatum, C. japonicum, and C. calceolus. The seed has a firm brown fusiform testa with an opening at one end where the micropyle of the ovule was situated. Cypripedium has small, fusiform, wind-dispersed seeds that weigh about $1{\sim}2{\mu}g$ and range from 0.1 to 2 mm long and 0.07 to 0.4 mm across. The germination of Cypripedium was previously thought to be hard to cultivate in one's garden and impossible to propagate artificially, nowadays lab-propagated seedlings are readily available in EU or United States. We had already reported the successful micropropagation of lady's slipper orchids with green pod sowing methods. The collected Cypripedium species and hybrids were planted in Korea National Arboretum (KNA) on Oct. 2018. The 11 species including C. candidum and the 31 hybrids such as C. Ingrid were introduced from Germany with CITES certification. The lady's slipper orchids bloomed on May to June, and the flowers were pollinated in the same species or hybrids to get seeds for in vitro germination on 2~3 days after fully flowering. The green pods were collected after 49~70 days after pollination. The 6 species of Cypripedium seeds were easily germinated on the POM within 70 days after sowing, and the 12 hybrids PLBs were observed with small roots. We had established the scheme of lady's slipper orchids germination in 3 months with green pods, and get the possibility of seedling in 10 months of sowing in vitro.

• 한국초지조사료학회지
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• 제9권3호
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• pp.179-186
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• 1989

사초용유채의 품종선별선급실험과 유채용유채와 사초용유채품종들 간에 있어서 생산성과 영양가치를 비교한 실험에서 사초용유채 도인품종중 우리나라 남부지역의 특성에 적합한 품종으로 Velox가 사초생산성 및 영양가치면에서 가장 유망하다고 생각되었다. 따라서 Velox를 공시하여 고립적배와 실행群落培 사이에 생산성과 영양가치에 있어서 어떤 차이가 있는가를 비교하였으며 또한 생육과정중의 영양가치의 변화를 조사하였는데 그 결과를 요약하면 다음과 같다. 1. 수당 생산성에 있어서 생체중과 건물중의 개체간 변이가 매우 컸으나 고립적배가 군락적배보다 1%수준의 유의차로 우수하였다. 孤립적배에 있어서 생체중과 건물중의 구성은 분지에 크게 의존되었으며 군락적배에 있어서는 분치와 주경이 비슷한 영향을 주었다. 건물율은 고리적배와 군락적배사이에 큰 차이가 없었으며 부위별로는 주경이 가장 높았다. 2. 粗蛋白質含量은 生育期間이 經過됨에 따라 점차 저하되었으며, 播種後 90일부터 120일까지에는 孤立적배가 群落적배보다 높은 반면 180일以後 개화기까지는 군락적배가 고립적배보다 높았다. NDF, ADF, cellulose 및 lignin등의 鐵維含量은 生育초기에는 낮은 水준이었으며 생育期間이 經過됨에 따라 점차 높아지면서 생育後期에는 孤立적배가 군락적배보다 높게 變化되었다. 3. In vitro 乾物소화率은 줄기나 잎 모두 生育기간이 경과됨에 따라 점차 저하되었으며, 줄기는 군락적배가 고립적배보다 높았으나 잎은 고립적배와 군락적배간에 유의차가 인정되지 않았다.

• The Plant Pathology Journal
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• 제29권1호
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• pp.52-58
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• 2013

Fusarium head blight (FHB) caused by the filamentous fungus Fusarium graminearum is one of the most severe diseases threatening the production of small grains. Infected grains are often contaminated with mycotoxins such as zearalenone and trichothecences. During survey of contamination by FHB in rice grains, we found a bacterial isolate, designated as BN1, antagonistic to F. graminearum. The strain BN1 had branching vegetative hyphae and spores, and its aerial hyphae often had long, straight filaments bearing spores. The 16S rRNA gene of BN1 had 100% sequence identity with those found in several Streptomyces species. Phylogenetic analysis of ITS regions showed that BN1 grouped with S. sampsonii with 77% bootstrap value, suggesting that BN1 was not a known Streptomyces species. In addition, the efficacy of the BN1 strain against F. graminearum strains was tested both in vitro and in vivo. Wheat seedling length was significantly decreased by F. graminearum infection. However, this effect was mitigated when wheat seeds were treated with BN1 spore suspension prior to F. graminearum infection. BN1 also significantly decreased FHB severity when it was sprayed onto wheat heads, whereas BN1 was not effective when wheat heads were point inoculated. These results suggest that spraying of BN1 spores onto wheat heads during the wheat flowering season can be efficient for plant protection. Mechanistic studies on the antagonistic effect of BN1 against F. graminearum remain to be analyzed.

• 농업과학연구
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• 제5권1호
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• pp.7-14
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• 1978

각종(各種) 살균제(殺菌劑)가 주요과수(主要果樹)의 화분발아(花粉發芽) 및 화분관신장(花粉管伸長)에 미치는 영향(影響)을 조사(調査)하였던바 다음과 같은 결과(結果)를 얻었다. 1. Difolatan-wp, Lime-Sulfur, Captan-wp, Bordeaux액(液)은 화분발아(花粉發芽) 현저(顯著)히 억제(抑制)시켰다. 2. Daconil-wp과 Zimaneb-wp가 미치는 영향(影響)은 Difolatan-wp등(等)에 비(比)하여 다소(多少) 가벼웠다. 3. Polyram-combi-wp, Topsin-wp, Benlate-wp는 발아(發芽)를 억제(抑制)하지 않았으나 화분관신장(花粉管伸長)을 다소(多少) 저해(沮害)하였다. 4. Polyoxin-wp는 오히려 화분관신장(花粉管伸長)을 촉진(促進)시켰다. 그러나 발아율(發芽率)의 증가는 없었다.

• 한국초지조사료학회지
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• 제25권1호
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• pp.17-22
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• 2005

This experiment was conducted to investigate the effect of seeding dates and rates on the productivity and nutritive value of hairy vetch(Vicia villosa Roth) at experimental field of Grassland and Forage Crops Division, National Livestock Research Institute, RDA, Suwon from 1999 to 2000. The experimental design was consisted of split-plot design with three replications. The main plot was seeding dates(25 Aug., 15 Sep. and 5 Oct.) and the subplot was seeding rates(20, 30 and 40 kg/ha). Late seeding dates increased the plant height and delayed the flowering stage as 1 or 2 days. The dry matter(DM) content was decreased with low seeding rates, and the trend was same in the all plots. Average crude protein(CP) content was $24.1\%$ and increased with delayed seeding date and lower seeding rate. The content of acid detergent fiber(ADF) and neutral detergent fiber(NDF) was increased with higher seeding rate, but seeding date did not affect. In vitro dry matter digestibility(IVDMD) of hairy vetch tended to decrease with high seeding rate, and total digestible nutrient(TND) did not show significant difference among seeding rates. Average relative feed value(RFV) of hairy vetch showed 140 which means very high quality. Dry matter and CP yield was decreased with delayed seeding date. The results of this experiments indicated that seeding in middle-August with 40 kg/ha would be recommended to produce the highest yield and quality f3r the cultivation of hairy vetch in middle part of Korea

• 화훼연구
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• 제18권3호
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• pp.207-210
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• 2010

'온누리'는 전라북도농업기술원에서 2006년 육성된 절화 및 분화용 아시아틱 나리이다. 1999년 오렌지색 품종인 'Avignon'를 모본으로 하고 노랑색 품종인 'Nove Cento'을 부본으로 교배하여 얻어진 실생 계통 중 화색과 화형이 안정적인 계통을 'A99-6-48'로 선발하였다. 2002~2003년 기내 조직배양을 통해 증식하여 포장에서 순화 및 양구하였으며 2004~2005년 2차 특성검정을 통해 'JLA99-7' 계통으로 선발되었고 2006년 최종 선발되어 '온누리'라 명명되었다. '온누리'의 화색은 orange-red group에 속하였으며 화피 내부의 중앙부는 orange group의 복색화이다. 초장은 92.8 cm, 화폭은 13.8 cm로 중륜이다. 주두의 색은 적갈색, 개화방향은 상향이며 개화기는 6월 중순이다.