Browse > Article
http://dx.doi.org/10.12791/KSBEC.2013.22.2.116

Selection of Crabapple Pollinizers for 'Fuji' Apple through Physiological and Genetic Analysis  

Son, KwangMin (Dept. of Horticultural Science, Kyungpook National Univ.)
Choi, Dong Geun (Department of Horticulture Science, Chonbuk National University)
Kwon, Soon-Il (Apple Research Station, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Kim, Byung Oh (Dept. of Applied Biology, Kyungpook National Univ.)
Choi, Cheol (Dept. of Horticultural Science, Kyungpook National Univ.)
Kang, In-Kyu (Dept. of Horticultural Science, Kyungpook National Univ.)
Publication Information
Journal of Bio-Environment Control / v.22, no.2, 2013 , pp. 116-122 More about this Journal
Abstract
We investigated characteristics and self-incompatibility genotypes of 11 crabapple cultivars to introduce a new pollinizer of 'Fuji' apple tree in Korea. Flowering dates of eleven crabapples were two to seven days earlier than that of 'Fuji'. The rate of pollen germination in vitro was ranged from 85.6% to 98.0% except 'Virginia'. Controlled pollination treatment with each crabapples to 'Fuji' increased fruit set rate about 20.4% to 34.4%, the number of seed per fruit about 13.8% to 42.3% and fruit weight about 7.4% to 16.7% compared to open pollination. Tested crabapples were resistant to peach fruit moth, brown leaf spot and sooty blotch in general. A PCR amplification method using S-RNase primers was carry out in eleven crabapples. S-alleles, $S_3$, $S_5$, $S_9$, $S_{10}$, $S_{20}$, $S_{26} from six crabapples were determinated. Through sequencing analysis, $S_5$ ('Manchurian', 'Virginia') and $S_9$ ('Yantaishagou') showed 100% homologous to previous result. Based on our results, it was recommended that 'Manchurian', 'Hopa A', 'Hanyaehanakaidou', 'Spectabilis' could be promising pollinzers for 'Fuji' apple cultivar.
Keywords
flowering date; pollen germination; S-genotype; fruit quality;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Alston, F.H. 1996. Incompatibility alleles and apple pollinaiton. Acta Hort. 423:119-124.
2 Church, R.M. and R.R Williams. 1971. Artificial pollination. J. Long Ashton Res. Sta. Rpt. for 1970:20.
3 Broothaerts, W., G.A. Janssens, P. Proost, and W.F. Broekaert. 1995. cDNA cloning and molecular analysis of two selfincompatibility alleles from apple. Plant molecular biology. 27:499-511.   DOI
4 Broothaerts, W. 2003. New finding in apple S-genotype analysis resolve previous confusion and request the re-numbering of some S-alleles. Theoretical and Applied Genetics 106:703-714.
5 Broothaerts, W., I.V. Nerum, and J. Keulemans. 2004. Update on and review of the incompatibility(S-) genotypes of apple cultivars. J. Hort. Sci. 39:943-947.
6 Crassweller, R.M., D.C. Ferree, and L.P. Nichols. 1980. Flowering crabapples as potential pollinizer for commecial apple cultivars. J. Amer. Soc. Hort. Sci. 105:475-477.
7 Florin, R. 1927. Pollen production and incompatibility in apples and pears. Mem. Hort. N.Y. 3:87-118.
8 Goldway, M., D. Schneider, H. Yehuda, A. Matityahu, D. Eisikowitch, and R.A. Stern. 2001. The effect of apple Sallele compatibility on fruit set levels in non-optimal fertilization conditions. Acta Hort. 561:231-234.
9 Golz, J.F., A.E. Clarke, and E. Newbigin. 1995. Self-incompatibility flowering plant. Curr. Opin. Genet. Dev. 5:640-645   DOI
10 Ha, Y.M. and K.K. Shim. 1995. Selection of new crabapple cultivars as pollinizers for apple orchard. J. Kor. Soc. Hort. Sci. 36:281-291.
11 Hegedus, A. 2006. Review of the self-incompatibility in apple. Intl. J. Hort. Sci. 12:31-36.
12 Paik, S.G. 1997. Evaluation of ornamental Malus species and cultivars as pollinators for single-cultivar commercial apple orchards. MS Thesis, Cornell Univ.
13 Howlett, F.S. 1926. Some factors of importance in fruit setting studies with apple varieties. Proc. Amer. Soc. Hort. Sci. 23:307-315.
14 Kang, I.K., G.J. Lee, M.J. Kim, S.I. Kwon, P.Y. Paek, and D.G. Choi. 2002. Selection of crab apples as pollinizers for major apple cultivars in apple orchard. Kor. J. Hort. Sci. Technol. 20:330-334.
15 Kang, I.K. 2004. Selection of crab apple as pollinizers for 'Hongro' apple cultivar. Kor. J. Hort. Sci. Technol. 22:212-215
16 Park, J.G., J.S. Hong, I.M. Choi, J.B. Kim, S.H. Kim, and H.S. Park. 1998. Applications of artificial pollination, spraying gibberellin $A_{4+7}$ plus benzyladenine for production of uniform fruits in 'Fuji' apples. Kor. J. Hort. Sci. Technol. 16: 27-29.
17 Sakurai, K., S.K. Brown, and N. Weede. 2000. Self-incompatibility alleles of apple cultivars and advanced selections. J. Hort. Sci. 35:116-119.
18 Schneider, D., R.A. Stern, D. Eisikowitch, and M. Goldway. 2001. Analysis of S-alleles by PCR for determination of compatibility in the 'Red Delicious' apple orchard. J. Hort. Sci. Biotech. 76:596-600.
19 Torres, A.M., N.F Weeden, and A. Martin. 1993. Linkage among isozyme, RFLP and RAPD markers in Vinciafaba. The oretical and Applied Genetics 85:937-945.
20 Way, R.D. 1978. Crabapple pollinizers. N.Y. State. Hort. Soc. Proc. 123:93-101.
21 Williams, R.R. 1967. A pollinator system for the single variety Cox's orange pippin orchard. Long Ashton Res. Sta. Rpt. 1966:112-114.
22 Williams, R.R. and R.M Church. 1983. Growth and flowering of ornamental Malus pollinators in apple orchards. J. Hort. Sci. 58:337-342.