• 제목/요약/키워드: in vitro antioxidant activity

검색결과 868건 처리시간 0.028초

The In Vitro Antioxidant Properties of Chinese Highland Lichens

  • Luo, Heng;Yamamoto, Yoshikazu;Liu, Yanpeng;Jung, Jae-Sung;Kahng, Hyung-Yeel;Koh, Young-Jin;Hur, Jae-Seoun
    • Journal of Microbiology and Biotechnology
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    • 제20권11호
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    • pp.1524-1528
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    • 2010
  • The antioxidant properties of 46 lichen species, collected from the highly UV-exposed alpine areas of southwestern China, were evaluated for their potential therapeutic utilization. The anti-linoleic acid peroxidation activity, 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging activity, reducing power, and total phenolic contents were all assessed in vitro in the methanol extract of the lichens. A potent reducing power was detected in a number of the lichen extracts, when compared with butylated hydroxyanisole (BHA). In general, it was found that many of the lichens, with antioxidant properties, contained large quantities of phenolic content. Extracts of Peltigera praetextata and Sticta nylanderiana were found to exhibit the most potent activity in all of the antioxidant tests. In particular, extracts of S. nylanderiana displayed a 1.37 times greater anti-linoleic acid peroxidation activity, when compared with the ascorbic acid used as the positive control. S. nylanderiana also possessed the strongest free radical scavenging activity amongst all the tested species, with an inhibition rate of 90.4% at concentration of $330{\mu}g/ml$. Activity-guided bioautographic TLC and HPLC analyses were used to establish which compounds were responsible for the potent antioxidant activities of the S. nylanderiana extract. These analyses revealed lecanoric acid to be primarily responsible for the effective antioxidant properties of S. nylanderiana. Overall, these results have indicated that several highland lichens have the potential of being utilized as novel bioresources for naturally occurring antioxidant therapies.

Antioxidant Activity-of Curcuma Longa L., Novel Foodstuff

  • Choi, Hae-Yeon
    • Molecular & Cellular Toxicology
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    • 제5권3호
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    • pp.237-242
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    • 2009
  • The potential antioxidant activities of different fractions from methanolic extract of Curcuma longa L. were assayed in vitro. All of the fractions exception of n-hexane and $H_2O$ showed a strong antioxidant activity, especially the ethylacetate (EtOAc) fraction, which showed the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity ($IC_{50}=9.86\;{\mu}g/mL$). The results of 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity and ferric reducing antioxidant power (FRAP) assay showed concentration dependency, the EtOAc fraction demonstrating a better result than the other fractions at the same concentration in this studies. Additionally, when the total phenolic contents of fractions were measured, the EtOAc fraction contained the highest level. Meanwhile, correlation analysis indicated a high correlation between the antiradical activity and the total phenolic contents, suggesting that fractions obtained from the methanolic extract of Curcuma longa L. have wide potential for use as sources of antioxidant material.

Influence of pH on the Antioxidant Activity of Melanoidins Formed from Different Model Systems of Sugar/Lysine Enantiomers

  • Kim, Ji-Sang;Lee, Young-Soon
    • Food Science and Biotechnology
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    • 제17권6호
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    • pp.1310-1315
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    • 2008
  • This study was to investigate the influence of pH on the antioxidant activity of melanoidins formed from glucose (Glc) and fructose (Fru) with lysine enantiomers in the Maillard reaction. Melanoidins formed from D-isomers were found to be effective antioxidants in different in vitro assays with regard to the ferrous ion chelating activity, 1, l-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging activities, ferric reducing/antioxidant power (FRAP), and 2,2'-azinobis(3-ethylbenothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity. In particular, the chelating activity of these melanoidins at a pH of 7.0 was greater than those with pH of 4.0 and 10.0. The chelating activity and DPPH radical scavenging activity of the melanoidins formed from the Glc systems were higher than those of the melanoidins formed from the Fru systems. However, the FRAP and ABTS radical scavenging activity of these melanoidins were not different according to pH level, with exceptions being the Fru systems.

홍차박 추출물의 in vitro 항산화 활성 (In vitro antioxidant activity of black tea (Camellia sinensis L.) residue extract)

  • 김동청
    • Journal of Applied Biological Chemistry
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    • 제62권3호
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    • pp.281-286
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    • 2019
  • 홍차를 우려내고 남은 부산물인 홍차박을 30% 에탄올로 추출하여 수율, 폴리페놀 함량 및 항산화 활성을 확인하였다. 홍차박의 추출 수율은 $22.4{\pm}1.18%$이었고, 폴리페놀은 추출물에 $23.2{\pm}1.02{\mu}g\;GAE/mg$ 들어있었다. 홍차박 추출물은 양이온라디칼, 유리라디칼 및 아질산염을 농도에 비례하여 소거하였고, 환원력과 지질과산화 억제활성도 농도의존적으로 증가하였다. 홍차박 추출물의 ABTS 양이온라디칼, DPPH 유리라디칼 및 아질산염 소거에 대한 $IC_{50}$값은 각각 141.8, 108.1 및 $397.2{\mu}g/m$이었고, 환원력에 대한 $IC_{50}$값은 $97.8{\mu}g/mL$로 나타났다. 또한 홍차박 추출물은 linoleic acid의 과산화를 효과적으로 억제하였다. 결론적으로 홍차박 추출물은 우수한 항산화 활성을 보유하고 있어 생리활성 소재로의 활용 가능성을 보여주었다.

Free Radical Scavenging and Antioxidant Activities of Water Extracts from Amannia multiflora, Amannia coccinea, Salix gracilistyla Inhabiting Along the Nakdong River (Republic of Korea)

  • Jayasingha Arachchige Chathuranga Chanaka Jayasingha;Mi-Hwa Lee;Chang-Hee Kang;Yung Hyun Choi;Gi-Young Kim
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2022년도 추계학술대회
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    • pp.31-31
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    • 2022
  • Plant-derived antioxidants are used as a healthy diet and are known to inhibit various human diseases. In this study, we investigated free radical scavenging and antioxidant activity of extracts from three plants (Ammannia multiflora, Ammannia coccinea and Salix gracilistyla) with the most DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity from 196 plant extracts inhabiting along Nakdong River in Republic of Korea. The three extracts also have strong total antioxidant activity. Moreover, the extracts inhibited hydrogen peroxide (H2O2)-induced reactive oxygen species production and depolarized mitochondrial membrane potential in RAW264.7 macrophages. In zebrafish larvae, 2',7'-dichlorodihydrofluorescein diacetate (DCFDA) fluorescent intensity, induced by H2O2, was markedly reduced by the extracts of A. multiflora, A. coccinea and S. gracilistyla. Meanwhile, the extracts were upregulated Nrf2 and HO-1 expression, and an HO-1 inhibitor reversed the extract-induced oxidative responses both in vivo and in vitro. The data suggest that the extracts of A. multiflora, A. coccinea, and S. gracilistyla exert potential free radical scavenging and antioxidant capacities both in vivo and in vitro by activating the Nrf2/HO-1 signaling pathway.

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Prebiotic Potential of Xylooligosaccharides Derived from Corn Cobs and Their In Vitro Antioxidant Activity When Combined with Lactobacillus

  • Yu, Xiuhua;Yin, Jianyuan;Li, Lin;Luan, Chang;Zhang, Jian;Zhao, Chunfang;Li, Shengyu
    • Journal of Microbiology and Biotechnology
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    • 제25권7호
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    • pp.1084-1092
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    • 2015
  • In the present work, the in vitro prebiotic activity of xylooligosaccharides (XOS) derived from corn cobs combined with Lactobacillus plantarum, a probiotic microorganism, was determined. These probiotics exhibited different growth characteristics depending on strain specificity. L. plantarum S2 cells were denser and their growth rates were higher when cultured on XOS. Acetate was found to be the major short-chain fatty acid produced as the end-product of fermentation, and its amount varied from 1.50 to 1.78 mg/ml. The antimicrobial activity of XOS combined with L. plantarum S2 was determined against gastrointestinal pathogens. The results showed that XOS proved to be an effective substrate, enhancing antimicrobial activity for L. plantarum S2. In vivo evaluation of the influence of XOS and L. plantarum S2, used both alone and together, on the intestinal microbiota in a mouse model showed that XOS combined with L. plantarum S2 could increase the viable lactobacilli and bifidobacteria in mice feces and decrease the viable Enterococcus, Enterobacter, and Clostridia spp. Furthermore, in the in vitro antioxidant assay, XOS combined with L. plantarum S2 possessed significant 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis, and superoxide anion radical-scavenging activities, and the combinations showed better antioxidant activity than either XOS or L. plantarum S2 alone.

Porcine Splenic Hydrolysate has Antioxidant Activity in vivo and in vitro

  • Han, Kyu-Ho;Shimada, Kenichiro;Hayakawa, Toru;Yoon, Taek Joon;Fukushima, Michihiro
    • 한국축산식품학회지
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    • 제34권3호
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    • pp.325-332
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    • 2014
  • The antioxidant capacity of porcine splenic hydrolysate (PSH) was studied in vitro and in vivo. Peptide hydrolysates were prepared, using the proteolytic enzyme $Alcalase^{(R)}$. The molecular weights of PSH were 37,666, 10,673, 6,029, and 2,918 g/mol. Rats were fed a 5% (w/v) PSH diet, instead of a casein diet, for 4 wk. The food intake, body weight gain, and liver weight of rats in the PSH group were similar to those in the control (CONT) group. There were no differences in the serum total cholesterol, triglyceride, total protein, or albumin levels between PSH and CONT groups. However, the level of in vivo hepatic lipid peroxidation in PSH group was significantly lower than that in CONT. In vivo hepatic catalase and glutathione peroxidase activities in the PSH group were significantly higher than those in the control group. The in vitro protein digestibility of PSH was lower than that of casein. The in vitro trolox equivalent antioxidant capacity of PSH was significantly higher than that of the peptide hydrolysate from casein. The in vitro radical scavenging activities of PSH were significantly higher than those of the peptide hydrolysate from casein. The present findings suggest that porcine splenic peptides improve the antioxidant status in rats by enhancing hepatic catalase and GSH-Px activities, and indicate a potential mechanism of radical scavenging activity during gastrointestinal passage.

Cytotoxic and antioxidant properties of four plants belonging to the genus Solanum

  • Dongre, Santoshkumar H.;Badami, Shrishailappa;Godavarthi, Ashok;S., Mahendran;P., Vijayan;B., Suresh
    • Advances in Traditional Medicine
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    • 제8권1호
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    • pp.86-92
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    • 2008
  • The aim of the study was to evaluate in vitro antioxidant and cytotoxic activities of the methanolic extracts of leaves of Solanum sisymbrifolium, Solanum anguivi multiflora, Solanum barbisetum and Solanum jasminoides. In the in vitro antioxidant screening using ABTS [2,2'-azino-bis (3-ethylbenzo-thiazoline-6-sulphonic acid) diammonium salt] method, the methanol extracts of Solanum jasminoides, Solanum barbisetum and Solanum anguivi multiflora exhibited potent antioxidant activity with $IC_{50}$ values 31.25 $\pm$ 0.35, 40.33 $\pm$ 0.57 and 54.33 $\pm$ 0.57 ${\mu}g$/ml, respectively. Solanum barbisetum also showed potent activity in DPPH [1,1-diphenyl-2-picryl hydrazyl] method with an $IC_{50}$ value of 55.33 $\pm$ 1.66 ${\mu}g/ml. In the cytotoxicity studies, the methanol extract of Solanum barbisetum exhibited moderate activity against Vero, HEp-2, HeLa and A-549 cell lines with $IC_{50}$ values in the range of 83.30 - 127.30 ${\mu}g$/ml. Solanum anguivi multiflora extract also showed moderate activity against Hep-2 cell line with $IC_{50}$ value of 80.13 ${\mu}g$/ml. Solanum barbisetum possessing both the activities requires further investigation.

Antioxidant Activities of Different Parts of Synurus deltoids Nakai Extracts in Vitro

  • Jung, Mee-Jung;Heo, Seong-Il;Wang, Myeong-Hyeon
    • Food Science and Biotechnology
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    • 제17권6호
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    • pp.1156-1159
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    • 2008
  • The antioxidant activity of hot water extracts of various parts, the leaf, stem, and root of Synurus deltoides was evaluated by various antioxidant assays, including total phenolic content, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, hydroxyl radical (${\cdot}OH$) scavenging, superoxide dismutase (SOD), and xanthine oxidase (XOI) activities. The various antioxidant activities were compared with the standard antioxidants such as L-ascorbic acid, $\alpha$-tocopherol, and butylated hydroxyanisole (BHA). Among the different plant parts, stem has been found to possess the highest activity in all tested model systems, the activity decreased in the order stems>roots>leaves. These results indicate that stem extract could be used as potential source of natural antioxidant.

Antioxidant Activities of Naturaceuticals Extract In Vitro

  • Park, Sung-Jin;Kwon, Woo-Taeg;Rha, Young-Ah
    • 한국조리학회지
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    • 제20권5호
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    • pp.29-33
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    • 2014
  • In this study, we examined the antioxidant activity of the naturaceutical extract in vitro using total phenolic contents, total flavonoids contents, DPPH radical scavenging activity, reducing power assay, and phenolic acid contents. The total phenolic and total flavonoids contents of naturaceutical extract were 5.46 mg/g, 2.21 mg/g, respectively. The DPPH radical scavenging activity of natraceutical extract varied from 18.77 ($200{\mu}g/mL$) to 3.44 ($1,000{\mu}g/mL$). The reducing power of the natraceutical extract absorbance varied from 1.07 (0.78 mg/mL) to 3.44 (12.5 mg/mL), and reducing power of extract presented a concentration-dependent activity increase. The highest amounts of trans-ferulic acid, p-hydroxybenzoic acid, p-coumaric acid, vanillin, vanillic acid, p-hydroxybenzaldehyde, and trans-cinnamic acid were observed in the naturaceutical extract at the levels of 750.79, 619.75, 531.34, 222.04, 219.28, 107.40, and $89.56{\mu}g/g$, respectively. The results imply that this antioxidant effect of the naturaceuticals extract could be harnessed in the management and prevention of degenerative diseases associated with oxidative stress.