Molecular & Cellular Toxicology

The Korean Society of Toxicogenomics and Toxicoproteomics (대한독성유전 단백체학회)
권호 표지

Antioxidant Activity-of Curcuma Longa L., Novel Foodstuff

  • Choi, Hae-Yeon (Department of Food and Nutrition, Sookmyung Women's University)
  • Published : 2009.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

The potential antioxidant activities of different fractions from methanolic extract of Curcuma longa L. were assayed in vitro. All of the fractions exception of n-hexane and $H_2O$ showed a strong antioxidant activity, especially the ethylacetate (EtOAc) fraction, which showed the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity ($IC_{50}=9.86\;{\mu}g/mL$). The results of 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity and ferric reducing antioxidant power (FRAP) assay showed concentration dependency, the EtOAc fraction demonstrating a better result than the other fractions at the same concentration in this studies. Additionally, when the total phenolic contents of fractions were measured, the EtOAc fraction contained the highest level. Meanwhile, correlation analysis indicated a high correlation between the antiradical activity and the total phenolic contents, suggesting that fractions obtained from the methanolic extract of Curcuma longa L. have wide potential for use as sources of antioxidant material.

Keywords

References

  1. Liu, S. et al. Intake of vegetables rich in carotenoids and risk of coronary heart disease in men: the physicians' health study. Int J Epidemiol 30:130-135 (2001) https://doi.org/10.1093/ije/30.1.130
  2. Bazzano, L. A. et al. Fruit and vegetable intake and risk of cardiovascular disease in US adults: the first national health and nutrition examination survey epidemiologic follow-up study. Am J Clin Nutr 76:93-99 (2002) https://doi.org/10.1093/ajcn/76.1.93
  3. Sies, H. Oxidative stress: oxidants and antioxidants. Exp Physiol 82:291-295 (1997) https://doi.org/10.1113/expphysiol.1997.sp004024
  4. Halliwell, B. & Gutteridge, J. M. Role of free radicals and catalyticmetal ions in human disease: an overview. Method Enzymol 186:1-85 (1990) https://doi.org/10.1016/0076-6879(90)86093-B
  5. Kahkonen, M. P. et al. Antioxidant activity of plant extracts containing phenolic compounds. J Agric Food Chem 47:3954-3962 (1999) https://doi.org/10.1021/jf990146l
  6. Sugihara, N., Arakawa, T., Ohnishi, M. & Furuno, K. Anti- and prooxidative effects of flavonoids on metal induced lipid hydroperoxide dependent lipid peroxidation in cultured hepatocytes located with $\alpha$-linolenic acid. Free Radic Biol Med 27:1313-1323 (1999) https://doi.org/10.1016/S0891-5849(99)00167-7
  7. Lorenzo, Y. et al. The carotenoid beta-cryptoxanthin stimulates the repair of DNA oxidation damage in addition to acting as an antioxidant in human cells. Carcinogenesis 30:308-314 (2009) https://doi.org/10.1093/carcin/bgn270
  8. Grice, H. C. Safety evaluation of butylated hydroxyanisole from the perspective of effects on forestomach and oesophageal squamous epithelium. Food Chem Toxicol 26:717-723 (1988) https://doi.org/10.1016/0278-6915(88)90072-5
  9. Sherwin, E. R. Antioxidants. In: Branen AL, Davidson PM, Salminen S (eds) Food additives. Marvel Dekker inc, New York, p. 139-193 (1990)
  10. Tang, W. & Eisenbrand, G. Chinese Drugs of Plant Origin. SpringerVerlag, Berlin Heidelberg, Germany (1992)
  11. Lee, H. S. Antiplatelet property of Curcuma longa L. rhizome-drived ar-turmerone. Bioresource Technol 97:1372-1376 (2006) https://doi.org/10.1016/j.biortech.2005.07.006
  12. Kim, M. K., Choi, G. J. & Lee, H. S. Fungicidal property of Curcuma longa L. rhizome-drived curcumin against phytopathogenic fungi in greenhouse. J Agr Food Chem 51:1587-1581 (2003)
  13. Jilani, G. & Saxena. R. C. Repellent and feeding deterrent effects of turmeric oil, sweetflag oil, neem oil, and neem-based insecticide against lesser grain borer (Coleptera: Bostrychidae). J Econ Entomol 83:629-634 (1990) https://doi.org/10.1093/jee/83.2.629
  14. Lee, H. S. et al. Insecticidal activities of ar-turmerone identified Curcuma longa L. rhizome against Nilaparvata lugens (Homoptera: Delphacidae) and Plutella xyostella (Lepidoptera: Yponomeutidae). J Asia-Pacific Entomol 4:181-185 (2001) https://doi.org/10.1016/S1226-8615(08)60121-1
  15. Ammon, H. P. T. & Wahl, M. A. Pharmacology of Curcuma longa. Planta Med 57:1-7 (1991) https://doi.org/10.1055/s-2006-960004
  16. Ramírez-Tortosa, M. C. et al. Oral administration of a turmeric extract inhibits LDL oxidation and has hypocholesterolemic effects in rabbits with experimental atherosclerosis. Atherosclerosis 147:371-378 (1999) https://doi.org/10.1016/S0021-9150(99)00207-5
  17. Devasagayam, T. P. A., Kamat, J. P. & Sreejayan, N. Antioxidant action of curcumin. In Micronutrients and Health: Molecular Biological Mechanisms. Nesaretnam K, Packer L (eds). AOCS Press: Champaign, IL, USA, p. 42-59 (2001)
  18. Devasagayam, T. P. A. & Sainis, K. B. Immune system and antioxidants, especially those derived from Indian medicinal plants. Indian J Exp Biol 40:639-655 (2002)
  19. Cuvelier, M. E., Richard, H. & Berset, C. Comparation of the antioxidative activity of some acid-phenols: Structure-activity relationships. Biosci Biotech Bioch 56:324-325 (1992) https://doi.org/10.1271/bbb.56.324
  20. Katalinic, V., Milos, M., Kulisic, T. & Jukic, M. Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols. Food Chem 94:550-557 (2006) https://doi.org/10.1016/j.foodchem.2004.12.004
  21. Wong, C. C., Li, H. B., Cheng, K. W. & Chen, F. A. Systematic survey of antioxidant activity of 30 chinese medicinal plants using the ferric reducing antioxidant power assays. Food Chem 97:705-711 (2006) https://doi.org/10.1016/j.foodchem.2005.05.049
  22. Buenger, J. et al. An interlaboratory comparison of methods used to assess antioxidant potentials. Int J Cosmet Sci 28:135-146 (2006) https://doi.org/10.1111/j.1467-2494.2006.00311.x
  23. Rice-Evans, C. A., Miller, N. J. & Paganga, G. Structure-antioxidant activity relationships of flavonoids and phenolic acid. Free Radical Biol Med 20:933-956 (1996) https://doi.org/10.1016/0891-5849(95)02227-9
  24. Bors, W. & Michel, C. Chemistry of the antioxidant effect of polyphenols. Ann NY Acad Sci 957:57-69 (2002) https://doi.org/10.1111/j.1749-6632.2002.tb02905.x
  25. Pozharitskaya, O. N., Ivanova, S. A., Shikov, A. N. & Makarov, V. G. Separation and free radical-scavenging activity of major curcuminoids of Curcuma longa using HPTLC-DPPH method. Phytochem Anal 19:236-243 (2008) https://doi.org/10.1002/pca.1035
  26. Reddy, A. C. & Lokesh, B. R. Studies on the inhibitory effects of curcumin and eugenol on the formation of reactive oxygen species and the oxidation of ferrous iron. Mol Cell Biochem 137:1-8 (1994) https://doi.org/10.1007/BF00926033
  27. Sreejayan, N. & Rao, M. N. Curcuminoids as potent inhibitors of lipid peroxidation. J Pharm Pharmacol 46:1013-1016 (1994) https://doi.org/10.1111/j.2042-7158.1994.tb03258.x
  28. Ak, T. & Gülçin, I. Antioxidant and radical scavenging properties of curcumin. Chem Biol Interact 174:27-37 (2008) https://doi.org/10.1016/j.cbi.2008.05.003
  29. Gue, J. & Wang, M. H. Antioxidant and antidiabetic activities of Ulmus davidiana extracts. Food Sci Biotechnol 16:55-66 (2007)
  30. Re, R. et al. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio Med 26:1231-1237 (1999) https://doi.org/10.1016/S0891-5849(98)00315-3
  31. Benzie, I. F. F. & Strain, J. J. The ferric reducing ability of plasma (Frap) as a measure of antioxidant power the Frap assay. Anal Biochem 239:70-76 (1996) https://doi.org/10.1006/abio.1996.0292
  32. Swain, Y. & Hillis, W. E. The phenolic constituents of Prunus Domestica I-the quntitative analysis of phenolic constituents. J Sci Food Agr 10:63-68 (1959) https://doi.org/10.1002/jsfa.2740100110