• Title/Summary/Keyword: in vitro absorption

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Absorption Behavior in the Body of Chitosan Oligosaccharide according to Molecular Weight; An In vitro and In vivo Study

  • Jang, Mi-Kyeong;Kang, Seong-Koo;Nah, Jae-Woon
    • Food Science and Biotechnology
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    • v.15 no.6
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    • pp.937-941
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    • 2006
  • Chitosan has a wide range of applications in biomedical materials as well as in dietary supplements. Chitosan oligosaccharide with free-amine group (COFa) is an improvement over traditional chitosan that lacks the usual impurities and materials detrimental to the body. Based on a previous study of water soluble chitosan (WSC, chitosan lactate), we investigated the molecular weight (Mw) - dependent absorption phenomena of COFa in vitro and in vivo with various Mws. The absorption of CO Fa was significantly influenced by its molecular weight. As Mw increases, the absorption decreases. The absorption profiles for 5 K COFa (Mw=5 kDa) were observed to be more than 10 times higher than those of high molecular weight chitosan (100 K HWSC Mw=100 kDa) in both in vitro and in vivo transport experiments. Furthermore, the in vitro transport experiment suggested that transcellular transport of the COFa (Mw <10 kDa) through Caco-2 cell layer could occur with a negligible cytotoxic effect. The COFas showed a cytotoxic effect on Caco-2 cells that was dependent on dose and Mw. COFa could be transported transcellularly through the Caco-2 cell layer.

A Suppressive Effect of Dietary Fiber on in Vitro Absorption of Lead (납의 In Vitro 흡수에 미치는 식이 섬유의 억제효과)

  • Lee, Su-Rae;Lee, Kyung-Sook
    • Korean Journal of Food Science and Technology
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    • v.21 no.1
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    • pp.63-67
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    • 1989
  • In order to examine the suppressive effect of dietary fiber toward the intestinal absorption of lead, an in vitro absorption test using a semipermeable membrane was undertaken. Among dietary fiber components, cellulose showed no suppressive effect, guar gum and carboxymethyl-cellulose, a slight effect whereas citrus pectin and sodium alginate exhibited a remarkable effect. Among fibrous foods tested, rice bran, wheat bran, Chinese cabbage, radish and tangle had a higher suppressive effect while mandarin orange, apple and laver showed a lower effect.

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Effect of Amoxicillin on the Intestinal Membrane Permeability and Absorption Clearance of Benazepril (베나제프릴의 장관막 투과도와 흡수 클리어런스에 미치는 아목시실린의 영향)

  • Joo, Eun-Hee;Kim, Young-Man;Ko, Hyung-Seok;Lee, Yong-Bok;Na, Han-Kwang
    • Journal of Pharmaceutical Investigation
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    • v.28 no.1
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    • pp.25-33
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    • 1998
  • Intestinal absorption of ${\beta}-lactam$ antibiotics and angiotensin converting enzyme(ACE) inhibitors has been shown to use the carrier-mediated transport system. In vitro experiments have established that the efficacy of uptake by enterocytes depends on an inwardly directed proton gradient. It was suggested that benazepril was mediated by tripeptide transport system and that amoxicillin was transported by dipeptide transport carrier. The aim of this study is to assess the influence of amoxicillin on the intestinal absorption of benazepril using in vitro diffusion chamber and in situ single pass perfusion technique in the rat in order to elucidate whether the above transport systems are competitive or not. We obtained the gastrointestinal pemeability coefficient of amoxicillin, benazepril and both of them using in vitro diffusion chamber. And also the gastrointestinal absorption clearance of amoxicillin, benazepril and both of them using in situ single-pass perfusion method at steady state were calculated. Amoxicillin and benazepril were analyzed by HPLC. The results by the use of diffusion chamber in vitro indicated that the apparent intestinal permeability coefficient of benazepril was significantly(p<0.01) decreased by amoxicillin(45.2%) and vice versa significantly(p<0.01) decreased(89.1%). The results by the in situ gastrointestinal single-pass perfusion method indicated that the intestinal absorption clearance of benazepril was significantly(p<0.05) decreased by amoxicillin (40.2%) and vice versa significantly(p<0.05) decreased(54.8%). These results might suggest that they share the same peptide carrier pathway for oral absorption.

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The Bile Acid Absorption Activity of Chitosan Derivatives (키토산 유도체의 담즙산 흡착 활성)

  • Lee, Ai-Leen;Lee, Kang-Man
    • YAKHAK HOEJI
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    • v.42 no.6
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    • pp.572-575
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    • 1998
  • Chitosan has been known to have hypocholesterolemic and hypolipidemic effects in animal studies. Chitosan also absorbs bile acids in vitro and in vivo, which might result in the hypocholesterolemic action. Trialkyl chitosan derivatives were prepared and tested for bile acid absorption activity in vitro. The derivatives showed enhanced absorption capacities which were comparable to cholestyramine.

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Dissoultion and Rectal Absorption of Acetaminophen from Suppositories (아세트아미노펜 좌제의 용출과 직장흡수)

  • 한정선;심창구;김신근
    • YAKHAK HOEJI
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    • v.31 no.5
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    • pp.286-295
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    • 1987
  • The relationship between in vitro release and in vivo bioavailability of acetaminophen from suppositories was investigated. Effect of glycyrrhizin on the drug release and rectal absorption in rats was also examined. Suppositories containing 25mg of acetaminophen were prepared with Wecobee FS (fatty base) or PEG (water-soluble base) bases. The release from the suppositories were determined with USP rotating basket dissolution apparatus and with the suppository release tester. The temperature of the dissolution medium was very critical for the dissolution of acetaminophen from Wecobee FS suppositories. The bioavailability of acetaminophen was calculated from the plasma concentration-time curve after rectal administration of the suppositories to the rats. There were no significant differences in AUC following rectal administration of Wecobee FS and PEG suppositories, but the release and absorption from the Wecobee FS suppositories were faster than those from PEG suppositories. The dissolution rate obtained by the suppository release tester was better correlated with in vivo absorption rate constant than that by the USP dissolution apparatus. It suggests that the partitioning between rectal fluid and suppository base is the rate-limiting step in the rectal absorption of acetaminophen from suppositories. Glycyrrhizin was found not to affect in vitro dissolution and rectal absorption of acetaminophen.

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A Suppressive Effect of Alginate on the Intestinal Absorption of Cadmium in vitro (카드뮴의 장내흡수(腸內吸收)에 미치는 알진산(酸)의 억제(抑制)효과)

  • Yang, Jae-Seung;Hahn, Sung-Hee;Lee, Su-Rae
    • Journal of Nutrition and Health
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    • v.11 no.3
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    • pp.9-12
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    • 1978
  • An effect of alginate and seaweeds on the intestinal absorption of cadmium was tested in vitro. The absorption of cadmium was remarkably suppressed by alginate though the effect was not selective toward cadmium or calcium. The suppressive effect was also observed with tangle and laver, differing in some aspects from alginate only.

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The Relationship of in vitro Dissolution and Intestinal Membrane Permeability with in vivo Bioavailability (시험관내 용출 및 장관막 투과도와 생체이용률과의 상관성)

  • 서수경;손수정;박인숙;최기환;김순선;유태무;조혜영;이용복;김동섭
    • YAKHAK HOEJI
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    • v.44 no.5
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    • pp.424-431
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    • 2000
  • A biopharmaceutics drug classification system for correlation between in vitro dissolution and in vivo bioavailability is proposed based on recognizing that drug dissolution and gastrointestinal permeability are the fundamental parameters controlling the rate and extent of drug absorption. The objective of this study was to assess whether in vitro dissolution profiles of immediate-release beta-blocker tablets can be correlated with intestinal membrane permeability and/or in vivo bioavailability In vitro dissolution of the beta-blocker tablets was examined using KP VII Apparatus II methods at various pH. Intestinal membrane permeability was determined in vitro using the diffusion chamber method. Bioavailablity parameters were cited from literatures. The dissolution profiles did not accurately represent the in vivo bioavailablity However there were good correlations between intestinal membrane permeability and log P (noctanol/buffer). The correlations obtained in this study indicated that in vitro diffusion chamber method could be used to predict intestinal absorption in vivo.

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Drug Interaction of Sulfamethazine and Ethanol (에탄올과 Sulfamethazine의 약물상호작용)

  • Choi, Jun-Shik;Chun, Jong-Churl;Lee, Jin-Hwan;Yu, Young-Jong
    • Journal of Pharmaceutical Investigation
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    • v.16 no.1
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    • pp.31-35
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    • 1986
  • Effect of ethanol on the absorption rate, blood level and bioavailability of sulfamethazine (SM) in rats was determined. Absorption rate of SM was determined both by the in vitro and in situ experiment. In vitro, absorption rate of SM in rat small intestine was increased by 0.3, 1.0 and 3.0% ethanol. In situ, absorption rate of SM was increased by 0.3 and 1.0% ethanol but not by 3.0% ethanol. After oral administration, blood level of SM was elevated and relative bioavailability was significantly increased to 114.8% at the dose of 0.6g/kg ethanol but not significantly at the dose of 3.0g/kg ethanol. The time for attainment of peak blood level was changed from 2.5 to 1.5hr. Ethanol enhanced absorption rate constant of SM significantly and reduced elimination rate constant of SM administered orally at the dose of 0.6g/kg ethanol.

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Calcium Absorption Accelerating Effect of Chitosnn Oligosaccharides prepared by Ultrafiltration Membrane Enzymatic Reactor (한외여과막 효소반응기를 이용하여 제조한 키토산 올리고당의 칼슘흡수 촉진효과)

  • JEON You-Jin;KIM Gyu-Hyung;PARK Pyo-Jam;KIM Se-Kwon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.32 no.3
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    • pp.247-251
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    • 1999
  • In spite of various bio-functionalities of chitosan, the effects in vivo were still ambiguous because of its low absorption on organism. Therefore, chitosan oligosaccharides (COSs) are necessary to elucidate for an efficient utilization in vivo. COSs were prepared from chitosan using ultrafiltration membrane enzymatic reactor system with MWCO (molecular weight cut-off) 3,000 Da of membrane. Calcium absorption accelerating effect using COSs was examined by two methods, in vitro and in vivo. In vitro experiment, calcium absorption by the addition of COSs in a mixture solution of calcium and phosphate was higher approximately $50\%$ than that by control. In vivo using rats, group taken the diet contained $1\%$ COSs anil calcium chloride decreased about $75\%$ of calcium content excreted from feces, and then, showed about $15\%$ increase in breaking force of femur. These results demonstrated that COSs definitely involved in calcium metabolism in vivo.

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