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Biological Activities in the Extract of Fermented Codonopsis lanceolata (발효더덕 추출물의 생리활성)

  • Park, Sung-Jin;Song, Seong-Won;Seong, Dong-Ho;Park, Dong-Sik;Kim, Seung-Seop;Gou, Jinyu;Ahn, Ju-Hee;Yoon, Won-Byung;Lee, Hyeon-Yong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.8
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    • pp.983-988
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    • 2009
  • To investigate the applicability of hot water extract from Codonoposis lanceolata (CL) and fermented Codonoposis lanceolata (FCL) as functional food and cosmeceutical material, its total phenolic contents, total flavonoids, electron donating ability (EDA), SOD-like activity, nitrate-scavenging ability (NSA), reducing power were examined. Total phenolic contents of CL and FCL were 0.54 mg/100 g and 2.79 mg/100 g, respectively, and total flavonoids contents were estimated as 2.26 mg/100 g for CL and 6.16 mg/100 g FCL. The EDA of CL and FCL were 8.0$\sim$17.9% and 32.9$\sim$74.9%. The SOD-like activities of CL and FCL were 0.6$\sim$16.5% and 3.5$\sim$21.6%, respectively, and the activity was dependent on the sample concentration. The NSA was pH dependent, and was the highest at pH 1.2 and the lowest at pH 6.0. The NSA of FCL was higher than that of CL. The FCL extract showed the highest reducing power (0.65) at the concentration of 1,000 $\mu g$/mL. Based on the above results, we deemed that the FCL might have potential antioxdant activities.

The Effects of Steroid on Acute Lung Injury in the Mouse Induced by Whole Lung Irradiation (전폐조사로 유발된 마우스의 급성폐손상에 대한 스테로이드의 효과)

  • Sung, Nak-Kwan;Shin, Sei-One;Kwon, Kun-Young
    • Radiation Oncology Journal
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    • v.15 no.1
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    • pp.37-47
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    • 1997
  • Purpose : To investigate ultrastructural changes of the mouse lung induced by whole lung gamma irradiation and to evaluate the effect of prophylactic administration of steroid against acute lung injury. Materials and Methods :. One hundred and twenty ICR mice were used and whole lung was irradiated with telecobalt machine. Whole lung doses were 8 and 12Gy, and 10mg of methyl prednisolone was administrated intraperitoneally for two and four weeks. At the end of the observation period, mice were sacrificed by cervical dislocation. The lungs were removed and fixed inflated. Histopathological examination of acute radiation injuries were Performed by light microscopic and transmission electron microscopic examination. Results : Control group with BGy is characterized by damage to the type I Pneumocyte and the endothelial cell of the capillary. edema of alveolar wall and interstitium. and fibroblast proliferation. Control group with 120y is characterized by more severe degree of type 1 pneumocyte damage and more prominant inflammatory cell infiltration. Destructed cell debris within the alveolar space were also noted After steroid administration, 8Gy experimental group showed decreased degree of inflammatory reactions but fibroblast proliferation and basal lamina damages were unchanged. Experimental group with 12Gy showed lesser degree of inflammatory reactions similar to changes of 8Gy experimental group. Conclusion : These studies suggest that the degree of interstitial edema and inflammatory changes were related to radiation dose but Proliferation of the fibroblast and structural changes of basal lamina were not related to radialion dose. Experimental administration of steroid for 2 to 4 weeks after whole lung irradiation suggest that steroid can suppress alveolar and endothelial damages induced by whole lung irradiation but Proliferation of the fibroblast and structural changes of basal lamina were not related to administration of steroid.

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Landscape Assessment and Improvement of the View Area by Selecting the Landscape Control Point (조망점 선정을 통한 대상지의 경관가치 평가 및 개선방안)

  • Kim, Jin-Hyo;Lee, Hyun-Taek;Ra, Jung-Hwa;Cho, Hyun-Ju;SaGong, Jung-Hee
    • Journal of the Korean Institute of Landscape Architecture
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    • v.40 no.5
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    • pp.19-32
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    • 2012
  • The purpose of this study is to quantitatively measure the view target and the view area through the selection of landscape control point by selecting Jimyeong-dong Dong-gu and Yeongyeong-dong Buk-gu Daegu, which are planned as the large scale housing complex development area, as the target places. It is very meaningful that from the simulation based on this measurement, the improvement methods are attempted to be suggested at the project level. The results of this study are as follows. First, the total number of viewing targets derived from the literature analysis for the selection of the viewing targets was 24, and finally derived main viewing targets were 4 places. Second, the total number of selection criteria of the derived landscape control point was 15, and these were re-categorized as prospect, accessibility and publicness according to the common property. The preliminary LCPs were selected by measuring the publicness and accessibility, and because of the said measurement, a total of 43 preliminary LCPs were selected. The final LCPs were selected by estimating the prospect of the selected preliminary LCPs, and as a result of estimation, a total of 29 final LCPs were selected. Finally, the total number of evaluation indicators derived from literature analysis was 26. Because of the valuation by the landscape control point, it was found that the 2 view areas were the I grade, 3 areas were II grade and 3 areas were the V grade, the lowest grade among 29 view areas. From the analysis on problems for the improvement methods, 4 improvement-indicators including the diversity of land mosaic were selected for the view area-1 without considering the development project drawing. In addition, for the view area-2 with considering the development project drawing, the landscape as the scenery forests was well formed, and the arrangement of architectures for the security of view corridor was right angle arrangement, and their floor number was 10.

Precipitation of $CaCO_3$ Crystals from Variously Supersaturated Solutions (다양한 과포화 조건하에서의 탄산염광물의 합성에 대한 연구)

  • Hyeon Yoon;Kim, Soo-Jin
    • Journal of the Mineralogical Society of Korea
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    • v.17 no.1
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    • pp.11-21
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    • 2004
  • Crystallization of CaC $O_3$ from the solutions of various degrees of supersaturation was carried out by a spontaneous precipitation method. The solution was kept at $25^{\circ}C$ and pH 6.9∼8.8. The solution compositions were varied in two ways: (1) The total carbonate, [C $O_3$]$_{Τ}$, to total calcium. [Ca]$_{Τ}$, ratios vary as ; [C $O_3$]$_{Τ}$/[Ca]$_{Τ}$ >1. [C $O_3$]$_{Τ}$/[Ca]$_{Τ}$=1, and [C $O_3$]$_{Τ}$/[Ca]$_{Τ}$<1. (2) The total calcium concentration, [Ca]$_{Τ}$, held at 0.02 mo1/d $m^3$, 0.2 mo1/d $m^3$, and 0.4 mo1/d $m^3$. We found that the CaC $O_3$ phase crystallized from the solutions of [C $O_3$]$_{Τ}$/[Ca]$_{Τ}$ $\geq$ 1 was mostly calcite with less than 1% of vaterite, while the CaC $O_3$ crystals precipitated from low carbonate concentration toward calcium concentration, [C $O_3$]$_{Τ}$/[Ca]$_{Τ}$ < 1, were dominated by vaterite crystals. It appears that the polymorph of CaC $O_3$ precipitate was mainly controlled not by the calcium concentration but by the carbonate concentration during the spontaneous precipitation. Also, we found that the surface roughness of vaterite increased with decreasing carbonate concentration from 0.8 or 0.5 of [C $O_3$]$_{Τ}$/[Ca]$_{Τ}$ ratios and the surface area of vaterite increased from 5.64∼7.34 $\mu\textrm{m}$ to 8.39∼10.3 $\mu\textrm{m}$.

A Study on Designation Potential as Ramsar Site and Management Method of Massive Scale of Wetland - A Case of Jang Hang Estuary Wetland, Han River, Korea - (대규모 습지의 Ramsar Site 지정 가능성 검토 및 관리방안 연구 - 한강하류 장항습지를 대상으로 -)

  • Yeum, Jung-Hun;Han, Bong-Ho;Lee, Kyong-Jae
    • Korean Journal of Environment and Ecology
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    • v.24 no.3
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    • pp.249-257
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    • 2010
  • The purpose of this paper is to weigh the possibility of endangered Jang-hang wetland at the estuary of Han River to be included on the Ramsar List of Wetlands of International Importance and come up with ways to manage the wetland depending on its biotope patterns. The target area is located between Gimpo bridge and Isanpo I.C. with about $2.7km^2$ area. Through the analysis of RIS(Information Sheet for Ramsar Wetlands), it was known that the wetland is located on the sedimentary topography and formed as a result of sediment at the estuary of the river owing to the concentration of rainfall during summer. The vegetation environment in the area is divided into brackish water and fresh water areas depending on salinity. Rhizosphere soil(RS) of the area was analyzed to be Silt loam while bottom RS to be Sand loam. The plant ecology was composed of 52 families 135 species and 11 varieties and 146 types. Among indigenous species found are Salix koreensis, Phragmites communis and Miscanthus sacchariflorus. The analyzed results of the actual vegetation showed that willow community accounts for 37% of the area and rice field is 13.5%. As for animal ecology, total of 62 species and 25,977 individual wild birds were observed. After comparing and analyzing the RIS we compiled with the Ramsar Site designation standards, it turns out that the Jang-hang wetland meets criteria 1(biographic region), criteria 2,3 and 4(species and ecological communities) and criteria 5 and 6(water birds). Thus, Jang-hang wetland is eligible for the Ramsar site. As a result of establishing and evaluating the biotope types for setting management areas, Jang-hang wetland has a total of 13 different types, and the grade I represents 75.4% of the area while the grade III 0.8% of the land status. We categorized four management zones for the wetland depending on the biotope patterns - preservation, restoration, use and buffer zones and suggested management methods for each zone.

Disappearance of Organic Phosphate Insecticides Residue on Vegetables and Fruit Crops (과실 및 채소류에 대한 유기 인계 농약의 잔류소장)

  • Woo, Ki-dae
    • Korean Journal of Agricultural Science
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    • v.4 no.2
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    • pp.229-238
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    • 1977
  • Sumithion and EPN residues on grapes, EPN and Diazinon on chinese cabbage, Parathion on peaches, Dimethoate on tomatoes, and EPN and Malathion on cucumber were analyzed in terms of 0, 3, 7, 14, 21 and 30 days after last application for the pesticides safty use. From the disappearance rate for various organo-phosphate insecticides on vegetables and fruit crops, following results are obtained. 1. On Chinese cabbage, Diazinon residues were 0.25~0.38p.p.m three weeks after one application, and EPN were 1.39~2.69p.p.m seven days after one application and 0.96~2.34p.p.m two weeks after twice application. 2. EPN residues on grapes were 1.09~1.80p.p.m seven days after one application and Sumithion were 0.17~0.53p.p.m fourteen days after one application. 3. On peaches, Parathion residues were 0.40~0.61p.p.m two weeks after last application. 4. Dimethoate residues on tomatoes were 0.141p.p.m seven days after four times application. 5. On cucumber, EPN residues were 2.11~2.14p.p.m three days after twice application, and Malathion were 0.46p.p.m 3 day after four times application but 0.062~0.025p.p.m three days after last application. 6. Rate of degradation of organo-phosphate chemicals is inversely related to half-life of its. 7. Minimum intervals between last treatment and harvest to prevent unsafty residues are as follows. 7 days for EPN with one application and 14 days with twice application on chinese cabbage, 3 days on cucumber and 7 days on grape, 14 days for parathion, 7 days for dimethoate on tomatoes, 0 to 3 days for Malathion on cucumber, 21 days for Sumithion on grape, 21 days on chinese cabbage for Diazinon.

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Studies on Changes of the Blood Pictures and Serum Components according to the Gestation Period in Rabbits (가토(家兎)의 임신기간(妊娠期間)에 따른 혈액상(血液像)과 혈청성분(血淸成分)의 변화(變化)에 관(關)한 연구(硏究))

  • Lee, Kyu Seung;Suh, Gil Woong
    • Korean Journal of Agricultural Science
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    • v.6 no.2
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    • pp.148-157
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    • 1979
  • The followings are the results obtained from the investigation of blood pictures and serum components of rabbits according to gestation period. 1. The erythrocytes count and hematocrit values were insignificantly decreased with the progress of gestation period. The hemoglobin content was significantly decreased at 3- and 4-week after gestation. 2. The total leukocytes count was continuousely increased during the gestation period. This tendency was significantly recognized at 3- and 4- week after gestation, but the normal situation was restored after parturition. While the percentage of neutrophils was significantly increased, that of lymphocytes was decreased from 3 weeks after gestation. 3. The contents of total protein and non-protein nitrogen were continuously decreased with the process of gestation period, but the significant differences were recognized from 3 weeks. 4. The total lipids were not markedly changed until 3 weeks, but significantly increased at 4-week after gestation and on 5-day after parturition. 5. The serum cholesterol tended to be decreased until 3 weeks, but significantly increased at 4-week after gestation and on 5-day after parturition. 6. The serum calcium was continuousely decreased during the gestation period, but the significant differences were recognized at 3- and 4-week. The serum phosphorus was also significantly decreased at 4-week after gestation.

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Antimicrobial Activity of Extracts from Different Parts and Essential Oil from Pinus densiflora on Skin Pathogens (소나무 부위별 추출물 및 essential oil의 피부상재균에 대한 항균 활성)

  • Park, Sun-Hee;Kim, Koth-Bong-Woo-Ri;Kim, Min-Ji;Choi, Jung-Su;Cho, Young-Je;Ahn, Dong-Hyun
    • Journal of Life Science
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    • v.27 no.6
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    • pp.646-651
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    • 2017
  • This study was carried out to examine the antimicrobial activity of 70% ethanol and hot water extracts from different parts and essential oil in Pinus densiflora on skin pathogens such as Staphylococcus epiermidis, S. aureus, Propionibacterium acnes, Candida albicans, C. tropicalis, and Trichophyton rubrum. The antimicrobial activities of extracts and essential oil were tested by paper disc assay and minimum inhibitory concentration test. The ethanol extract of pine pollen showed antimicrobial activity against C. tropicalis and T. rubrum. Ethanol extract of pine needle and pine gnarl showed antimicrobial activity against S. aureus, C. tropicalis, and P. acnes. Essential oil from pine needle exhibited antimicrobial activity against C. albicans, C. tropicalis, and P. acnes. The minimum inhibitory concentration of ethanol extracts of pine needle and pine gnarl against P. acnes and C. tropicalis ranged from 0.002% to 0.0063%. The lowest minimum inhibitory concentration (0.025%) against C. albicans and C. tropicalis was obtained from essential oil. These results indicate that ethanol extracts of pine pollen, pine needle, pine gnarl, and essential oil of pine needle could be applicable to control the skin infection pathogens. Especially, ethanol extract of pine gnarl had a broad spectrum of antimicrobial activity and pine extracts and essential oil exhibited higher antimicrobial activity with Candida sp. and P acnes.

Effects of N-Methylacetamide on the Viability, Fertility and Hatchability of Cryopreserved Ogye (Korean Native Black Fowl) Semen (N-Methylacetamide 동결보호제가 오계 동결정액의 생존성, 수정 및 부화율에 미치는 영향)

  • Choi, Jin Seok;Kim, Sung Woo;Shin, Dan-Bi;Ko, Yeoung-Gyu;Do, Yoon-Jung;Kim, Dong-Hun;Kong, Il-Keun;Park, Soo-Bong
    • Korean Journal of Poultry Science
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    • v.39 no.4
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    • pp.291-295
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    • 2012
  • The use of methylacetamide (MA) as a cryoprotective agent for freezing Korean Native Black rooster Ogye semen was examined with artificial insemination. The diluted Ogye semen with HS-1 was subjected for 2 step dilution method of cryopreservation in which the final concentration of MA was adjusted to 7.5%. The sperm viability after thawing was reduced from $95.17{\pm}0.93%$ to $55.93{\pm}1.38%$ which was confirmed by live-death analysis based on Fluorescence-Activated Cell Sorting (FACS). The rates of fertilized eggs with fresh or frozen-thawed semen were reduced from $94.98{\pm}3.93%$ to $66.36{\pm}8.43%$ at day 7 with significant difference. However, the hatching rates of experiments at day 21 did not shown difference between $92.64{\pm}2.33%$ and $90.45{\pm}8.05%$ (P<0.05). With these results, the utilization of MA for freezing of Ogye spermatozoa could affect on viability of frozen-thawed semen but not on the fertility of lain eggs and hatchability of fertilized eggs and also provide possible tools of freezing for poultry genetic resource conservation.

A Study on the Factors Affecting the Usage Intentions of 5G Mobile Communication Service (5G 서비스의 이용의도에 영향을 미치는 요인에 관한 연구)

  • Jeong, Man Soo;Hong, Dae Sik;Ji, Yong Gu
    • The Journal of Society for e-Business Studies
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    • v.25 no.1
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    • pp.135-176
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    • 2020
  • This study is to investigate the factors affecting the usage intentions of 5G service between 5G service subscribers and non-subscribers based on the Integratively Extended Technology Acceptance Model in the initial B2C 5G service market. We designed the integratively extended technology acceptance model to find the more specific factors to affect the usage intentions by integrating the exogenous factors including the technical factors, the social factors, and the personal factors with the ETAM(Extended Technology Acceptance Model). Referring to the previous studies, we constructed the hypotheses of structural influence relationship from the exogenous factors to the dependent usage intentions factors through the mediating belief factors. We designed the 5G service usage intentions model based on these hypotheses and operational definitions of the factors. Using the analysis of SEM(Structural Equation Model), the proposed model and the hypotheses were empirically tested by the data collected from 245 subscribed respondents and 245 non-subscribed respondents. As a result, the perceived ease of use has an effect on the perceived usefulness and enjoyment, and the perceived usefulness/enjoyment and the personal innovativeness have positive effects on the usage intentions of both groups.