• 제목/요약/키워드: in vitro protein digestibility

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Metabolisable Energy, In situ Rumen Degradation and In vitro Fermentation Characteristics of Linted Cottonseed Hulls, Delinted Cottonseed Hulls and Cottonseed Linter Residue

  • Bo, Y.K.;Yang, H.J.;Wang, W.X.;Liu, H.;Wang, G.Q.;Yu, X.
    • Asian-Australasian Journal of Animal Sciences
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    • 제25권2호
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    • pp.240-247
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    • 2012
  • Dietary supplementation with conventional linted cottonseed hulls (LCSH) is a common practice in livestock production all over the world. However, supplementation with mechanically delinted cottonseed hulls (DCSH) and cottonseed linter residue (CLR) is uncommon. Cottonseed by-products, including LCSH, DCSH and CLR, were assessed by chemical analysis, an in situ nylon bag technique, an in vitro cumulative gas production technique and in vitro enzyme procedure. The crude protein (CP) content of CLR (302 g/kg dry matter (DM)) was approximately 3 times that of LCSH and 5 times that of DCSH. The crude fat content was approximately 3 times higher in CLR (269 g/kg DM) than in LCSH and 4 times higher than in DCSH. Neutral detergent fibre (311 g/kg DM) and acid detergent fibre (243 g/kg DM) contents of CLR were less than half those of DCSH or LCSH. Metabolisable energy, estimated by in vitro gas production and chemical analyses, ranked as follows: CLR (12.69 kJ/kg DM)>LCSH (7.32 kJ/kg DM)>DCSH (5.82 kJ/kg DM). The in situ degradation trial showed that the highest values of effective degradability of DM and CP were obtained for CLR (p<0.05). The in vitro disappearance of ruminal DM ranked as follows: CLR>LCSH>DCSH (p<0.05). The lowest digestibility was observed for DCSH with a two-step in vitro digestion procedure (p<0.05). The potential gas production in the batch cultures did not differ for any of the three cottonseed by-product feeds. The highest concentration of total volatile fatty acids was observed in CLR after a 72 h incubation (p<0.05). The molar portions of methane were similar between all three treatments, with an average gas production of 22% (molar). The CLR contained a higher level of CP than did LCSH and DCSH, and CLR fermentation produced more propionate. The DCSH and LCSH had more NDF and ADF, which fermented into greater amounts of acetate.

조미훈연 오징어의 가공중 품질변화 (Changes in Quality of Seasoned and Smoked Squid During Processing)

  • 류흥수;문숙임;이강호
    • 한국수산과학회지
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    • 제25권5호
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    • pp.406-412
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    • 1992
  • 조미훈연 오징어(Neon flying squid, Qmmastrephes bartrannii)의 적절한 가공조건을 찾기 위하여 일반성분변화 및 단백질 품질변화를 측정하였다. 수분, 조단백질함량은 매우 감소하였으나(p<0.05) 지방, 회분함량은 그다지 증가하지 않았다. 조미, 훈연은 TBA가의 증가를 초래하였다. 여러 가지 가공단계들은 TI함량을 크게 증가시키지는 않았다. 오징어 가공전단계를 통해 측정된 TI함량은 같은 가공단계에서의 TBA가와 서로 관련이 없었다. Steak용으로 가공한 것을 제외한 모든 가공품들의 경우, 소화율과 C-PER, DC-PER이 증가하였다. 생시료의 경우, 북태평양산 빨강오징어(몸통, 다리 양쪽 모두)의 효소 소화율은 다른 오징어종에 비해 상당히 낮았다.

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혼파초지의 이용방법 비교시험 II. 방목 및 예취이용이 목초의 품질과 토양경도에 미치는 영향 (Trials of the Utilization Method in Mixtures Swards II. Effects of grasing and cutting management on foragequality and soil hardness)

  • 신재순;이필상;박근제;윤익석
    • 한국초지조사료학회지
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    • 제10권2호
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    • pp.96-101
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    • 1990
  • This field experiment was undertaken to find out the effect of grazing and cutting management on the forage quality and soil hardness at different legumes sown swards of the experimental field of Livestock Experiment Station in Suweon, from August 1985 to October 1988. The results obtained are as follows: 1. Crude protein, crude fat and ash contents under grazing were higher than these under cutting, but showed the reverse results in crude fiber and nitrogen free extracts contents. Average in vitro dry matter digestibility (IVDMD) under grazing (72 %) was a little higher than that under cutting (71 %). On the other hand, there was little difference among the mixtures swards in mineral contents and IVDMD. 2. The contents of Ca, P and Mg were a little high in the cutting, but K and Na contents were in gazing. Ca: P ratio in grazing (2.97) was slightly higher than that of cutting (2.94). 3. Production of energies(TDN, StE and NEL) with cutting were appeard to increase by 19.4 %, 13.5 %and 19.0 % than those of grazing, respectively. Mixtures swards including alfalfa showed more production both grazing and cutting. 4. Soil hardness in the top lOcm of soil by cattle grazing was changed from 15.5mm at startihg year to 22.6 mm after 3 years, but in cutting slightly increase from 15.5mm to 16.2mm. Generally it was low in early spring and high in autumn.

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Aspergillus oryzae와 Bacillus subtilis를 순차접종 발효한 갈색거저리유충과 누에번데기 발효물의 품질특성 (Characteristics of Tenebrio molitor larvae and Bombyx mori pupae sequentially fermented with Aspergillus oryzae and Bacillus subtilis)

  • 강상훈;정장호
    • 한국식품과학회지
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    • 제54권1호
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    • pp.94-102
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    • 2022
  • 탈지 갈색거저리 유충과 번데기 분말을 A. oryzae 및 B. subtilis 균주를 이용하여 발효추출물을 제조하였다. 그 결과 발효추출물의 단백질 함량은 69.67-86.06% (p<0.001)이며, 액상발효물의 고형분 수율은 37.76-46.88% (p<0.001)로 나타났다. 단백질 분해율은 대두발효물(esS) 58.90%, 갈색거저리유충(esT; 52.62%), 누에번데기(esP; 50.13%) 순으로 나타났으며(p<0.001), 단백질 수율의 경우 47.47-63.02% 이였다(p<0.001). 또한 SDS-PAGE 결과, 발효과정을 거치지 않은 각각의 탈지원물과 비교 시 액상발효물들의 단백질이 저분자로 분해됨을 확인하였다. 액상발효물의 in vitro 소화율을 측정한 결과, 탈지원물(59.60-76.03%)에 비해 발효추출물의 단백질 소화율이 91.02-95.86%로 1.26-1.53배 증가하였다(p<0.001). 액상발효물의 단백질 용해도, 거품형성능, 거품안정성 모두 발효과정을 통해 수치가 증가하는 경향성을 나타났다. 이를 바탕으로 발효공정을 통한 단백질 추출물 공정의 가능성을 확인하였으며, 추후 추출물의 이화학적 특성 및 기능성을 기반으로 새로운 기능성 식품 소재 개발에 기여할 수 있을 것으로 보인다.

Addition of Novel Lactobacillus plantarum KCC-10 and KCC-19 to Improve Fermentation Quality and Characterization of Italian Ryegrass Silage

  • Srigopalram, Srisesharam;Ilavenil, Soundharrajan;Vijayakumar, Mayakrishnan;Park, Hyung Soo;Lee, Kyung Dong;Choi, Ki Choon
    • 한국초지조사료학회지
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    • 제35권3호
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    • pp.195-200
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    • 2015
  • An investigation was carried out to evaluate the potential role of Lactobacillus Plantarum KCC-10 and KCC-19 on the quality and fermentation characterization of Italian ryegrass (IRG) silages. The physiochemical properties of IRG silage such as crude protein content, neutral detergent fiber, acid detergent fiber, total digestible nutrient and in vitro dry matter digestibility were not affected by KCC-10 and KCC-19. The pH of IRG silage in KCC-10 and KCC-19 treatments decreased compared to the control (p<0.05), while the lactic acid content in KCC-10 and KCC-19 treatments increased compared to the control (p<0.05). In addition, the number of lactic acid bacteria (LAB) in the KCC-10 treatment increased compared to the control (p<0.05). The number of lactic acid bacteria in KCC-19 increased, but there was no significant difference in all treatments. Therefore, we recommend L. plantarum KCC-10 and KCC-19 as potential additive candidates in IRG silage with lots of advantages.

Effects of cultivar and harvest days after planting on dry matter yield and nutritive value of teff

  • Saylor, Benjamin A;Min, Doohong;Bradford, Barry J
    • Journal of Animal Science and Technology
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    • 제63권3호
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    • pp.510-519
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    • 2021
  • One of the most pressing issues facing the dairy industry is drought. In areas where annual precipitation is low, irrigation for growing feed presents the greatest water-utilization challenge for dairy producers. Here, we investigated the effects of cultivar and harvest days after planting (DAP) on dry matter (DM) yield and nutritive value of teff (Eragrostis tef), a warm-season annual grass native to Ethiopia that is well adapted to drought conditions. Eighty pots were blocked by location in a greenhouse and randomly assigned to four teff cultivars (Tiffany, Moxie, Corvallis, and Dessie) and to five harvest times (40, 45, 50, 55, or 60 DAP). Cultivars had no effect on DM yield and nutritive value. As harvest time increased from 40 to 60 DAP, DM yield and ash-free neutral detergent fiber (aNDFom) concentrations increased, while crude protein (CP) concentrations and in vitro NDF digestibility decreased. To assess carryover effects of time of harvest on yield and nutritive value, two additional cuttings were taken from each pot. Increasing first-cutting harvest time decreased CP concentrations in the second cutting and reduced DM yields in the second and third cutting. Harvesting teff between 45 and 50 DAP best optimized forage yield and nutritive value in the first and subsequent cuttings.

질소시비수준과 첨가제가 사초용 유채의 사료가치 및 사일리지의 품질에 미치는 영향 I. 예취시기 및 질소시비수준이 수량 및 사료가치에 미치는 영향 (Effects of Nitrogen Fertilization Levels and Some Additives on the Chemical Composition and Silage Quality of Forage Rape (Brassica napus Subsp. oleifera) I. Effects of harvestinh dates and N forage rape)

  • 조무환;김동암
    • 한국초지조사료학회지
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    • 제8권1호
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    • pp.33-39
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    • 1988
  • Insufficient herbage during the fall in the temperate areas of the world has been a serious limitation in animal production, but leafy brassicae are potentially very useful for extending the grazing season when the growth and quality of grass is poor. This study was conducted to determine the effects of cutting stage and N fertilization on dry matter (DM) yield, forage quality and chemical composition of forage rape (Brassica napus Subsp. oleifera). The experiment was conducted in the Experimental Livestock Farm of Seoul Nat'l Univ., at Suweon, during 1985- 1986. The results obtained are summarized as follows: 1. DM percentage and yield significantly increased as the growth period was prolonged and decreased as the N fertilization increased, but in-vitro dry matter digestibility (IVDMD) was unaffected by the treatments. 2. Plant height, DM yield and $NO_3$-N content significantly increased as the rate of N fertilization increased and DM percentage decreased, but the IVDMD was unaffected. 3. The forage rape had high percentage of crude protein and ash. Glutamic acid, pro!ine and aspartic acid were the major amino acids. Concentration of P, Ca and Mg were high.

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Evaluation of the nutritional value of locally produced forage in Korea using chemical analysis and in vitro ruminal fermentation

  • Ki, Kwang Seok;Park, Su Bum;Lim, Dong Hyun;Seo, Seongwon
    • Asian-Australasian Journal of Animal Sciences
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    • 제30권3호
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    • pp.355-362
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    • 2017
  • Objective: The use of locally produced forage (LPF) in cattle production has economic and environmental advantages over imported forage. The objective of this study was to characterize the nutritional value of LPF commonly used in Korea. Differences in ruminal fermentation characteristics were also examined for the LPF species commonly produced from two major production regions: Chungcheong and Jeolla. Methods: Ten LPF (five from each of the two regions) and six of the most widely used imported forages originating from North America were obtained at least three times throughout a year. Each forage species was pooled and analyzed for nutrient content using detailed chemical analysis. Ruminal fermentation characteristics were also determined by in vitro anaerobic incubations using strained rumen fluid for 0, 3, 6, 12, 24, and 48 h. At each incubation time, total gas, pH, ammonia, volatile fatty acid (VFA) concentrations, and neutral detergent fiber digestibility were measured. By fitting an exponential model, gas production kinetics were obtained. Results: Significant differences were found in the non-fiber carbohydrate (NFC) content among the forage species and the regions (p<0.01). No nutrient, other than NFC, showed significant differences among the regions. Crude protein, NFC, and acid detergent lignin significantly differed by forage species. The amount of acid detergent insoluble protein tended to differ among the forages. The forages produced in Chungcheong had a higher amount of NFC than that in Jeolla (p<0.05). There were differences in ruminal fermentation of LPF between the two regions and interactions between regions and forage species were also significant (p<0.05). The pH following a 48-h ruminal fermentation was lower in the forages from Chungcheong than from Jeolla (p<0.01), and total VFA concentration was higher in Chungcheong than in Jeolla (p = 0.05). This implies that fermentation was more active with the forages from Chungcheong than from Jeolla. Analysis of gas production profiles showed the rate of fermentation differed among forage species (p<0.05). Conclusion: The results of the present study showed that the nutritional values of some LPF (i.e., corn silage and Italian ryegrass) are comparable to those of imported forages widely used in Korea. This study also indicated that the nutritional value of LPF differs by origin, as well as by forage species. Detailed analyses of nutrient composition and digestion kinetics of LPF should be routinely employed to evaluate the correct nutritional value of LPF and to increase their use in the field.

Broken rice in a fermented total mixed ration improves carcass and marbling quality in fattened beef cattle

  • Kotupan, Salisa;Sommart, Kritapon
    • Animal Bioscience
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    • 제34권8호
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    • pp.1331-1341
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    • 2021
  • Objective: This study aimed to determine the effects of replacing cassava chips with broken rice in a fermented total mixed ration diet on silage quality, feed intake, ruminal fermentation, growth performance, and carcass characteristics in the final phase of fattening beef cattle. Methods: Eighteen Charolais-Thai native crossbred steers (average initial body weight: 609.4±46 kg; average age 31.6 mo) were subjected to three ad libitum dietary regimes and were maintained in individual pens for 90 d before slaughter. The experimental design was a randomized complete block design by initial age and body weight with six replicates. The dietary regimens used different proportions of broken rice (0%, 16%, and 32% [w/w] of dry matter [DM]) instead of cassava chips in a fermented total mixed ration. All dietary treatments were evaluated for in vitro gas production and tested in in vivo feeding trials. Results: The in vitro experiments indicated that organic matter from broken rice was significantly more digestible than that from a cassava-based diet (p<0.05). Silage quality, nutrient intake, ruminal fermentation characteristics, carcass fat thickness, and marbling score substantially differed among treatments. The ruminal total volatile fatty acids, propionate concentration, dietary protein intake, and digestibility increased linearly (p<0.05) with broken rice, whereas acetate concentration and the acetate:propionate ratio decreased linearly (p<0.05) with broken rice (added up to 32 g/kg DM). Broken rice did not influence plasma metabolite levels or growth performance (p>0.05). However, the marbling score increased, and the carcass characteristics improved with broken rice. Conclusion: Substitution of cassava chips with broken rice in beef cattle diets may improve fattened beef carcass quality because broken rice increases rumen fermentation, fatty acid biosynthesis, and metabolic energy supply.

Comparative nitrogen use efficiency of urea and pig slurry for regrowth yield and nutritive value in perennial ryegrass sward

  • Park, Sang Hyun;Lee, Bok Rye;Cho, Won Mo;Kim, Tae Hwan
    • Asian-Australasian Journal of Animal Sciences
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    • 제30권4호
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    • pp.514-522
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    • 2017
  • Objective: The study aimed to assess the N use efficiency (NUE) of pig slurry (in comparison with chemical fertilizer) for each regrowth yield and annual herbage production and their nutritive value. Methods: Consecutive field experiments were separately performed using a single application with a full dose of N (200 kg N/ha) in 2014 and by four split applications in 2015 in different sites. The experiment consisted of three treatments: i) control plots that received no additional N, ii) chemical fertilizer-N as urea, and iii) pig-slurry-N with five replicates. Results: The effect of N fertilization on herbage yield, N recovery in herbage, residual inorganic N in soil, and crude protein were significantly positive. When comparing the NUE between the two N sources (urea and pig slurry), pig slurry was significantly less effective for the earlier two regrowth periods, as shown by lower regrowth dry matter (DM) yield, N amount recovered in herbage, and inorganic N availability in soil at the 1st and 2nd cut compared to those of urea-applied plots. However, the effect of split application of the two N sources was significantly positive at the last two regrowth periods (at the 3rd and 4th cut). The two N sources and/or split application had little or no influence on neutral detergent fiber (NDF) content, acid detergent fiber (ADF) content, and in vitro DM digestibility, whereas cutting date was a large source of variation for these variables, resulting in a significant increase in in vitro DM digestibility for the last two regrowth periods when an increase in NDF and ADF content occurred. Split application of N reduced the N loss via nitrate leaching by 36% on average for the two N sources compared to a single application. Conclusion: The pig slurry-N was utilized as efficiently as urea-N for annual herbage yield, with a significant increase in NUE especially for the latter regrowth periods.