• Title/Summary/Keyword: in vitro protein digestibility

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Studies on the Improvements of Functional Properties of Sardine Protein by Plastein Reaction (Plastein반응을 이용한 정어리 단백질의 기능성 개선에 관한 연구 3. Plastein의 기능성 및 소화율)

  • Kim, Se-Kwon;Kwak, Dong-Chae;Cho, Duck-Jae;Lee, Eung-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.17 no.4
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    • pp.312-319
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    • 1988
  • The functional properties of plasteins have been compared with those of sardine protein concentrate and egg albumin. The solubility of plasteins was higher than that of FPG and the glu-plastein had 84% solubility in the range of pH 3-10. The dispersibility of plasteins was lower than that of egg albumin, however those of plasteins was higher than that of sardine protein concentrate. The water holding capacity of plasteins was higher than that of egg albumin. Lipid absorption of leu-papain plastein was the highest, holding 2.2m119, and that of the other plastein was higher than that of egg albumin. The emulsifying activity of leu-papain plastein was the highest, holding 66.4%, and that of glu-papain plastein was the lowest, holding 51.2%, The emulsifying stability of plasteins was similar to that of the emulsifying activity. The foaming capacitt of leu-papain plastein was the highest, holding 460%, and those of the other plasteins was higher than that of egg albumin. The foaming stability of plasteins was superior to that of egg albumin. The viscosity of plasteins was lower than that of see albumin. The in vitro digestibility of plasteins was 67.6-78.0% range. The digestibility by four pretense were somewhat lower in the glu-papain plastein than in the FPG. The digest of plasteins treated with the microbiol pretense such as molsin and pretense(from Streptomyces griceus), which had a storage broth taste.

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Quality Changes of Dried Lavers during Processing and Storage 3. Changes in Pigments, Trypsin Indigestible Substrates(TIS) and Dietary Fiber Content during Roasting and Storage (김의 가공 및 저장중의 품질변화 3. 배소 및 저장중의 색소, Trypsin 저해물질(TIS) 및 Dietary Fiber의 변화)

  • LEE Kang-Ho;RYUK Ji-Hee;JEONG In-Hak;JUNG Woo-Jin
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.23 no.4
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    • pp.280-288
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    • 1990
  • Quality stability of dried lavers during roasting and storage was investigated by measuring the changes of pigment contents including chlorophyll a, carotenoids and biliproteins, the content of trypsin indigestible substrates(TIS), in vitro apparent protein digestibility, and dietary fiber. In heat treatment or roasting of dried laver, carotenoids and chlorophyll a were found to be more stable than biliproteins. Chlorophyll a and carotenoids were retained more than $85\%$ during roasting for 1 hour at $120^{\circ}C$ while biliproteins were retained only $10\%$ at the same temperature. The in vitro digestility of dried layers tended to increase with raising the roasting temperature. The in vitro digestibility of $85\%$ for the roasted laver at $100^{\circ}C$ was higher than that observed in the control of $80\%$. There was a correlation between the decrease in TIS and biliproteins as the laver was roasted. The soulble dietary fiber(SDF) content was substantially increased by heat treatment. The extent of protein digestiblility appeared to be related to the increase of SDF content. In the storage of roasted lavers under both water activities 0.1 and 0.65, the loss of the pigments and TlS were markedly retarded at Aw 0.1. Chlorophyll a was retained about $20\%$ at aw 0.65 and $75\%$ at aw 0.1 after 20 week sto-rage. At worst, more than $90\%$ of the carotenoids were lost at aw 0.65 after 20 week, while biliproteins were comparatively stable at the same water activity. TIS decreased about $15\%$ and in vitro apparent protein digestibility increased up to $92\%$ at aw 0.65 during storage.

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Chemical Composition, Degradation Characteristics and Effect of Tannin on Digestibility of Some Browse Species from Kenya Harvested during the Wet Season

  • Osuga, I.M.;Abdulrazak, S.A.;Ichinohe, T.;Fujihara, T.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.1
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    • pp.54-60
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    • 2005
  • A study was conducted with the objective of evaluating the nutritive value of some browse species from Kenya. The species evaluated included: Bauhinia alba, Bauhinia variegata, Bridelia micrantha, Calliandra calothyrsus, Carisa edulis, Cratylia argentea, Gliricidia sepium, Lantana camara, Maerua angolensis, Sesbania micrantha and S. sesban. The browses were evaluated by their chemical composition including phenolics, in vitro gas production and tannin activity (tannin bioassay). All the species had high crude protein content (149-268 g/kg DM) and low NDF content (239-549 g/kg DM). The feeds had varying contents of total extractable tannins (TET) ranging from low (3-22 mg/g DM), moderate (42-58 mg/g DM) and high (77-152 mg/g DM). Calliandra calothyrsus had the highest tannin content. Significant (p<0.05) variation in gas production was recorded among the species. Sesbania micrantha had the highest (p<0.05) potential gas production while Gliricidia sepium had the highest (p<0.05) rate of gas production. Use of polyethylene glycol (PEG 6000), to assess the adverse affect of tannins, indicated that tannins in browse species with high tannin content had inhibitory effects on rumen microbial fermentation as indicated by the gas production. Estimated organic matter digestibility and metabolizable energy also increased with PEG addition. The results of this study indicate that such Kenyan browse species have the potential to be used as feed supplements for ruminant animals.

Effects of Slope Exposure and Altitude on Productivity of Orchardgrass in Mountain Pasture (산지초지에 있어서 경사방향 및 표고가 orchargrass의 생산성에 미치는 영향)

  • Woo-Bock Chun;Kwang-Hyun Kim
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.10 no.3
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    • pp.137-140
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    • 1990
  • This study was carried out to investigate the effects of slope exposure and altitude on the dry matter yield and on the grass quality in mountain pasture for 2-year period from 1986 to 1987. 1. Dry matter yield and crude protein content of grasses by slope exposure in mountain pasture were higher on the northern slope than that on the southern slope and, on both sides of slope, were increased as the altitude was high. 2. Neutral detergent fiber(NDF) and acid detergent fiber(ADF) contents, and in vitro dry matter digestibility were not different between different slope exposures and between different altitudes.

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Influence of Sowing Time on Growth, Yield and Nutritional Quality of Forage Rape in Spring

  • Kwon, Byung-Sun;Shin, Jeong-Sik;Park, Hee-Jin;Shin, Jong-Sup;Choi, Seong-Yu
    • Plant Resources
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    • v.6 no.1
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    • pp.11-15
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    • 2003
  • To determine as optimal sowing time of forage rape in spring time in southern areas of Korea, forage rape cv. Velox, the highest yielding variety among introduced varieties of forage rape, was grown under five different sowing times. Yield components such as plant length, number of branches and number of leaves etc. were higher at the sowing time of Mar. 15 and Mar. 25. The plants sown at Mar. 15 and Mar. 25 also showed highest fresh and dry matter yield. When plants were grown under later sowing time, they showed higher values in content of crude protein and lower values in contents of crude fiber such as Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF), cellulose and lignin. There was no relationship between variation of In Vitro Dry matter Digestibility (IVDMD) and sowing time. The plants sown at Mar. 15 and Mar. 25 showed highest digestible dry matter yields.

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Differences in Nutrient Quality among Wheat, Barley and Rye for Forage

  • Kwon Byung-Sun
    • Plant Resources
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    • v.8 no.1
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    • pp.75-79
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    • 2005
  • TO select the most suitable crop and variety of forage for nutrient quality at the southern part of Korea, the crop of wheat, barley and rye were grown from Oct. 1999 to June 2000. Paldanghomil variety of rye crop was shown to have the highest chemical components in comparison to other varieties of crops used in this experiment. It showed relatively high content of crude protein and in vitro dry matter digestibility (IVDMD) and low content of NDF, ADF, cellulose and lignin. Therefore, it was concluded that paldanghomil of rye crop was the most suitable variety with high weight and high nutrient quality for forage in the southern part of Korea. The heritabilities of all nutrient quality characters were estimated to be high.

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Protein Cross-linking in Freeze Dried Fish Meat (동결건조 어육의 단백질 Cross-linking에 관한 연구)

  • LEE Kang-Ho;JUNG Woo-Jin;SUH Jae-Soo;JEONG In-Hak;KIM Chung-Gon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.19 no.2
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    • pp.100-108
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    • 1986
  • This study was carried out in order to investigate protein cross-linking in freeze-dried meat of flounder (Limanda herzensteini). Changes in solubility or extractability of proteins and electrophoretic patterns of the extracted proteins were determined to monitor the cross-linking during the storage of freeze-dried meat. Development of nonenzymatic browning and the loss of in vitro protein digestibilily were also measured to assess their influences on the changes of functional and nutritional properties of proteins. In addition, the effects of lysine added, and removal of fat and water extractives were also mentioned. The extractability of protein decreased upon storage time and temperature, and the loss of solubility of myosin was evident. In case of the samples stored at $5^{\circ}C$ for 150 days, the extractability of protein decreased $26.4\%$, while that of the samples stored at $20^{\circ}C$ for 60 days decreased about $39.7\%$. And it was noted that the loss of solubility of myosin was $68.3\%$ and $98.1%$ for the same storage conditions, respectively. It was noteworthy that the samples treated with $L-lysine{\cdot}HCl$ seemed to prevent more or less the loss of protein solubility, in that, even stored at $20^{\circ}C$ for 120 days, revealed only $57.03\%$ decrease. The nonenzymatic browning was proceeded with the increase of storage temperature, especially, in the samples treated with glucose. This suggests that the decrease in extractibility of myosin was accompanied by the extent of browning. But the browning was retarded in defatted samples. The in vitro apparent protein digestibility was also higher in the samples defatted or water extracted. It was suggested from these results that changes in properties of proteins in freeze dried fish meat were led by the protein cross-linking which was attributed to Maillard type of reactions and protein-lipid interactions.

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Influence of Plant Density on Growth, Yield and Nutritional Quality of Forage Rye

  • Kwon, Byung-Sun
    • Plant Resources
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    • v.7 no.3
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    • pp.170-173
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    • 2004
  • To determine optimal plant density of forage rye in southern areas of Korea, forage rye cv. Paldanghomil, the highest yielding variety among experimental varieties of forage rye, was grown under five different plant density. Yield components such as plant height, and number of leaves were highest at the broad casting. Plants sown at broadcasting also showed highest fresh and dry matter yield. When plants were grown of the broad casting, they showed higher values in content of crude protein and lower values in contents of crude fiber such as neutral detergent fiber(NDF), acid detergent fiber(ADF), cellulose and lignin. There was no relationship between variation of in vitro dry matter digestibility(IVDMD) and plant density. Plants sown at the broad casting showed highest digestible dry matter yields.

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Studies of Cultivation Model of Rye (Secale cereale L.) as a Forage Crop I. Effects of harvesting time on forage production and quality of rye(Secale cereale L.) silage on paddies (호맥 ( Secale cereale L. ) 의 청예이용을 위한 재배모형에 관한 연구 I. 답리작호맥의 수확시기별 청예사료생산 및 Silage품질)

  • 송진달;임근발;양종성
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.8 no.3
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    • pp.165-168
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    • 1988
  • This study was conducted at the rice field of Livestock Experiment station, Suweon to investigate the effects of harvesting time on forage production and quality of rye (Secale cereale L.) on paddies. The results obtained are summarized as follows; 1. Fresh matter yield was peaked at the 10 days (May 10) after heading, however, dry matter yield was increased in proportion to maturity. 2. Digestibility (in vitro) showed the 83-77% by heading stage (Apr. 25-30) but decreased to 66-58% after heading stage. 3. Digestible yield reached to maximum level, 619.2 kg/lOa at the 10 days after heading (May 10). 4. The content of crude protein and crude fat in forage rye was decreased with advancing the maturity. 5. The lactic acid content of rye silage ranged from 1.3% to 2.0%.

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Changes in Functional Groups of Protein by Lipid Deterioration in the Biological System of Rice Bran (미강 저장 중 지방의 산패에 따라 생성된 산화 지질이 단백질의 기능기에 미치는 영향에 관한 연구)

  • Song, Yeong-Ok;Choi, Hong-Sik
    • Korean Journal of Food Science and Technology
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    • v.22 no.5
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    • pp.596-601
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    • 1990
  • The effects of peroxidized lipid on the protein in the biological system of rice bran was studied by determining the changes in the content of functional groups under two different storage conditions. One stored at controlled atmosphere of $35^{\circ}C$ with relative humidity 65% and the other one was exposed to the air of $25^{\circ}-30^{\circ}C$ with relative humidity 70-90%. The lipid peroxidation started after the lipolysis was almost completed. The autoxidation occurred much faster in the bran exposed to the air than that stored in the controlled atmosphere. Substantial changes in the physiochemical characteristics were observed in all of the major functional groups in both of the samples. The content of sulfhydryl and available lysine decrease·1 as lipid peroxidation progressed. Protease activity was lost almost completely. Protein solubility and in vitro digestibility also decreased during storage. The lipid peroxidation and contents of major protein functional groups were significantly correlated (p<0.05) and the correlation coefficients were higher than -0.8, for the both of the sample. peroxidized lipid was found to deteriorate protein in the biological system as well.

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