• Animal cells and systems
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• v.15 no.3
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• pp.251-258
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• 2011

Ciliates are considered one of the most diverse protozoa and play significant roles in ecology. For successful taxonomic study of these microscopic eukaryotes, a staining procedure is necessary, due mainly to intrinsic difficulties in recognizing characteristics from living cells. Although molecular taxonomy has been used to resolve the ambiguities associated with traditional morphology-based taxonomy, extraction of genomic DNA from stained ciliate cells is not available yet. In the present study, we describe a method to extract genomic DNA from a single protargol-impregnated euplotid cell. By using $HgCl_2$ as a fixative and modulating the exposure time of bleach solution in the protargol impregnation, high-quality genomic DNA can successfully be extracted from a stained single cell with minimal loss of morphological integrity. This technique will contribute to the effectiveness of combined approaches of molecular and morphological taxonomy from single ciliate cells.

• Clinical and Experimental Reproductive Medicine
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• v.2 no.1
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• pp.19-38
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• 1975

A total of 185 vasovasostomies were carried out for 11 years. Various factors which are of importance in fluencing the successful vasovasostomies are analysed and presented as follows: In recent years, demand of reanastomosis of the vas deferens increased considerably. An average age of the subjects is 39, and that of their wives, 33 in this series. An average interval between vasectomy and vasovasostomy is 4 years. An average number of living chidren is 2.6 at the time of vasectomy. and 2.1 at the time of time of vasovasostomy. Merchant and public official are the most commonly encounterd occupation in the present series. Coital frequencies are 2.2/week after vasovasostomy. The most common reasons for requesting the vasovasostomy are remarriage and deaths of children, especially son. Success rate is considerably higher among younger group than that of the older. Success rate is somewhat higher among groups of shorter interval between the operations (vasectomy and vasovasostomy) than that of longer interval group. Success rate is higher among bilateral vasovasostomy group than that of any others. Success rate is higher in solid splint group and no splint group than hollow splint group. The closed dressing technique of the end of splint on the scrotum is found to be reasonably effective in preventing infection. Ordinary end-to-end anastomosis and folded side-to-side anastomosis techniques are proved to be the most ideal form of modified operation for the successful vasovasostomies. The most common causes of failure are infection, injuries of blood supply, avascular necrosis due to extensive mobilization, inadequate approximation of both vasal end, hematoma, changes of epididymal environment, and early ambulation in this series. Overall success rate of the author's series is found the 81 per cent, and impregnation rate is 35 per cent. Overall results reported by other workers are that success rate regarding appearance of sperm in the semen after operation is 64 per cent, and that of impregnation after vasovasostomy is 33 per cent. The results of semen assessment performed on same cases prior to vasectomy and following vasovasostomy showed that post-vasovasostomy sperm count scarcely reached three-quaters of pre-vasectomy count.

• Journal of Species Research
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• v.6 no.spc
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• pp.15-25
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• 2017

Twelve ciliates were collected from freshwater, marine, and brackish water habitats in Korea and taxonomic descriptions are provided based on observations of living cells and protargol-impregnated specimens. These ciliates, recorded for the first time in Korea, were classified into 12 genera, 11 families, 10 orders, and five classes. We provide brief descriptions and remarks, including microphotographs, for the 12 ciliates: Allotricha mollis, Amphileptus eigneri, Didinium gargantua, Holophrya teres, Lacrymaria marina, Novistrombidium apsheronicum, Pelagostrobilidium conicum, Pseudochilodonopsis fluviatilis, Pseudourostyla subtropica, Strombidium conicum, Urocentrum turbo, and Uronema marinum.

• Journal of Species Research
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• v.7 no.2
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• pp.181-186
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• 2018

We investigated five ciliate species from soil, estuary and marine habitats in eastern Gangwon-do Province, South Korea. Morphology was studied through observation of living specimens as well as silver preparations. The species were classified into five genera, five families, five orders and four classes. Aspidisca aculeata, Hemiurosoma similis, Litonotus alpestris, Stentor roeselii and Vorticella infusionum are first records in Korea, and among them, the genus Hemiurosoma is recorded for the first time. Here, we provide short descriptions with microphotographs of these species.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.9
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• pp.6236-6246
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• 2015

In the paper, we reduce non-pregnant conditions and improve impregnation rate by unmanned estrus detection and decide proper time for artificial insemination. It is too hard to detect estrus only by using activities, we develop unmanned estrus detection system that consist of RF activity sensors, cow management program and estrus detection algorithm that uses information of activities and breeding. We verify performance by experiments in four similar scale stockbreeding farmhouse. Each stockbreeding farmhouse breeds 87, 81, 93, 82 cows and expected estrus cows are 14, 19, 15, 17. In expected estrus cow, we fail in weak estrus detection - 3, 2, 1, 3 cows, but detect successfully normal estrus - 11, 17, 14, 14 cows. After artificial insemination, 10, 17, 13, 14 cows became pregnant successfully confirming that proposed unmanned estrus detection system is effective for deciding proper time for artificial insemination in normal estrus.

• Korean Journal of Materials Research
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• v.22 no.6
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• pp.321-327
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• 2012

The production of functional activated carbon materials starting from inexpensive natural precursors using environmentally friendly and economically effective processes has attracted much attention in the areas of material science and technology. In particular, the use of plant biomass to produce functional carbonaceous materials has attracted a great deal of attention in various aspects. In this study the preparation of activated carbon has been attempted from rice husks via a chemical activation-assisted microwave system. The rice husks were milled via attrition milling with aluminum balls, and then carbonized under purified $N_2$. The operational parameters including the activation agents, chemical impregnation weight ratio of the calcined rice husk to KOH (1:1, 1:2 and 1:4), microwave power heating within irradiation time (3-5 min), and the second activation process on the adsorption capability were investigated. Experimental results were investigated using XRD, FT-IR, and SEM. It was found that the BET surface area of activated carbons irrespective of the activation agent resulted in surface area. The activated carbons prepared by microwave heating with an activation process have higher surface area and larger average pore size than those prepared by activation without microwave heating when the ratio with KOH solution was the same. The activation time using microwave heating and the chemical impregnation ratio with KOH solution were varied to determine the optimal method for obtaining high surface area activated carbon (1505 $m^2$/g).

• Applied Chemistry for Engineering
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• v.20 no.4
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• pp.430-436
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• 2009

Deactivated three-way catalysts which had been exposed to gasoline engine exhaust for a long time were remanufactured by ultra sonic cleaning with distilled water, sulfuric acid solution and impregnation with precious metals (Pt, Pd, Rh). The catalytic properties as well as conversion reactivity of CO, THC and NOx about fresh, aged and remanufactured catalysts were examined. Most of the pollutants deposited on the aged three-way catalysts were removed in the remanufacturing process of those catalysts. At the same time a little amount of precious metals like Pt and Pd were removed in the remanufacturing process. Under the experimental condition used in this study, in the case of the remanufactured catalysts with impregnation of precious metals, the catalytic activities were recovered to almost the same level, or higher level of that of the fresh catalyst.

• Journal of Mechanical Science and Technology
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• v.14 no.3
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• pp.320-330
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• 2000

It is desirable to perform nondestructive evaluation (NDE) to assess material properties and part homogeneity because the manufacturing of carbon/carbon brake disks requires complicated and costly processes. In this work several ultrasonic techniques were applied to carbon/carbon brake disks (322mm ad, 135mm id) for the evaluation of spatial variations in material properties that are attributable to the manufacturing process. In a large carbon/carbon disk manufactured by chemical vapor infiltration (CYI) method, the spatial variation of ultrasonic velocity was measured and found to be consistent with the densification behavior in CYI process. Low frequency (e.g., 1-5MHz) through-transmission scans based on both amplitude and time-of-flight of the ultrasonic pulse were used for mapping out the material property inhomogeneity. Images based on both the amplitude and the time-of-flight of the transmitted ultrasonic pulse showed significant variation in the radial direction. The radial variations in ultrasonic velocity and attenuation were attributed to a density variation caused by the more efficient densification of pitch impregnation near the id and od and by the less efficient densification away from the exposed edged of the disk. Ultrasonic velocities in the edges of the disk. Ultrasonic velocities in the thickness direction were also measured as a function of location using dry-coupling transducers ; the results were consistent with the densification behavior. However, velocities in the in-plane directions (circumferential and radial) seemed to be affected more by the relative contents of fabric and chopped fiber, and less by the void content.

• Journal of Korean Neurosurgical Society
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• v.38 no.4
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• pp.287-292
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• 2005

Objective : Kainic acid[KA] enhances the expression of nitric oxide synthase, increases nitric oxide[NO], and thus evokes epileptic convulsion, which results in neuronal damage in the rat brain. NO may stimulate cyclooxygenase type-2 [COX-2] activity, thus producing seizure and neuronal injury, but it has also been reported that KA-induced seizure and neurodegeneration are aggravated on decreasing the COX-2 level. This study was undertaken to investigate whether the suppression of NO using the NOS inhibitor, N-nitro-L-arginine methyl ester[L-NAME], suppresses or enhances the activity of COX-2. Methods : Silver impregnation and COX-2 immunohistochemical staining were used to localize related pathophysiological processes in the rat forebrain following KA-induced epileptic convulsion and L-NAME pretreatment. Post-injection survival of the rat was 1, 2, 3days and 2months, respectively. Results : After the systemic administration of KA in rats, neurodegeneration increased with time in the cornu ammonis [CA] 3, CA 1 and amygdala, as confirmed by silver impregnation. On pretreating L-NAME, KA-induced neuronal degeneration decreased. COX-2 enzyme activities increased after KA injection in the dentate gyrus, CA 3, CA 1, amygdala and pyriform cortex, as determined by COX-2 staining. L-NAME pretreatment prior to KA-injection, caused COX-2 activities to increase compared with KA- injection only group by 1day and 2days survival time point. Conclusion : These results suggest that L-NAME has a neuroprotective effect on KA-induced neuronal damage, especially during the early stage of neurodegeneration.

• Journal of the Korean Wood Science and Technology
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• v.43 no.6
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• pp.792-797
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• 2015

In this study, three softwood species were treated with water-soluble melamine resin by different concentration and treatment time under vacuum pressure for improving mechanical property, abrasion resistance, and incombustibility. After the treatment, a compreg was manufactured and then evaluated on physical properties. Additionally, incombustibility of compreg was determined by comparing with a wood that was treated by spraying a water-soluble fire retardant on surface. As concentration of resin increased, bending strength and Brinell hardness increased as well as abrasion resistance, but there was no correlation on treatment and mechanical properties by treatment time. The wood impregnated by water-soluble melamine resin under vacuum pressure showed better incombustibility than that of a water-soluble fire retardant sprayed wood. Therefore, this treatment could be used for improving incombustibility of wood.