• Korean Journal of Veterinary Research
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• v.40 no.3
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• pp.415-420
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• 2000

Distribution of S-100 protein-immunoreactive cells in the gastrointestinal tract of the catfish, Silurus asotus was investigated by PAP method. S-100 protein-immunoreactive cells were mainly observed just under the epithelium of the gastrointestinal tract. Immunoreactive cells were distributed numerously in the stomach and moderately in the middle part of the intestine, however, a few in the upper and lower part of the intestine.

• Korean Journal of Veterinary Research
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• v.38 no.3
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• pp.467-473
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• 1998

The relative frequency and distribution of bovine chromogranin(BCG)-, serotonin-, somatostatin(SOM)-, and S-100 protein-immunoreactive cells in the proventriculus, gizzard and proventriculus-gizzard junction(P-G junction) of the duck(Anas platyrhynchos platyrhyncos, Linne) were studied using immunohistochemical methods. BCG-, and SOM-immunoreactive cells were detected in all ages of this study in the proventriculus and the gizzard, and BCG-immunoreactive cells were also detected in the P-G junction. SOM-immunoreactive cells were detected rarely on occasions only in 1 week, 2 weeks, 3 weeks and 9 weeks after hatching. But no S-100 protein-immunoreactive cells were detected in this study.

• The Korean Journal of Zoology
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• v.30 no.2
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• pp.154-166
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• 1987

Recently, the researches on the enteroendocrine cells of vertebrates have made a remarkable advance by the immunocytochemical methods. This study was attempted to investigate the topographical distributions and the shapes of gastrin, glucagon, somatostatin and cholecystokinin-8 immuno-reactive cells in the gastrointestinal tract of the Korean hedgehog, Erinaceus koreanus. For light-microscopical examination of immunocytochemistry, the tissue specimens taken from the various portions(body and pyloric protion of stomach, duodenum, jejunum, ileum and rectum) were fixed in glutaraldehyde-picric acid-acetic acid (GPA) or 10% neutral buffered formalin solutions. For the demonstration of immunoreactive cells, the paraffin sections (6$\mu$m) were immunocytochemically identified by PAP procedure (Sternberger, 1979) with gastrin, glucagon, somatostatin and cholecystokinin-8 antisera. Gastrin-immunoreactive cells were mainly distributed in the pyloric portion of stomach and were a few in the duodenum and jejunum. The shapes of these cells were round or oval in the pyloric portion and pyramidal in the small intestine. Glucagon-immunoreactive cells were sparsely distributed in the only small intestine. The shapes of these cells were mainly pyramidal. Somatostatin-immunoreactive cells were a few in the pyloric portion and duodenum, and were sparsely distributed in the body of stomach and jejunum. The shapes of these cells were round or oval in the stomach and oval or pyramidal in the small intestine. Cholecystokinin-8-immunoreactive cells were sparsely distributed in the only small intestine. The shapes of these cells were mainly oval or pyramidal.

• Korean Journal of Veterinary Research
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• v.35 no.1
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• pp.39-44
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• 1995

Using immunohistochemical technique, three endocrine cell types were observed in the GIT of the Korean salamander, Hynobius leechii. BPP-immunoreactive cells were distributed from the pyloric region to the small intestine. A moderated number of somatostatin-immunoreactive cells were restricted to the pyloric region. 5-HT-immunoreactive cells were demonstrated throughout the GIT, reaching a peak in the pyloric region. The presence of BPP- and 5-HT-immunoreactive cells are identified for the first time in Hynobius leechii.

• Korean Journal of Veterinary Research
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• v.37 no.1
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• pp.9-13
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• 1997

The gastrointestinal endocrine cells of the African clawed toad, Xenopus laevis have been investigated immunohistochemically using the avidin-biotin method. Seven antisera were tested and three endocrine cell types immunoreacted with antisera to neurotensin, GRP and substance P. A moderate number of neurotensin-immunoreactive cells were weakly reacted in the small intestine. GRP-immunoreactive cells were mainly situated among the upper portion in the fundic glands, and the basal portion in the pyloric glands. These cells were oval and round in shape. On the other hand, in the intestine they were thin spindly cells with the epithelium. Substance P-immunoreactive cells were observed in among intestinal epithelium. However, no secretin-, motilin-, M-Enk- and PYY-immunoreactive cells were found in the GIT of the African clawed toads.

• Korean Journal of Veterinary Research
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• v.37 no.2
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• pp.253-262
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• 1997

To investigate morphological changes in the endocrine pancreas of chicken after pancreatic duct ligation, experimental animals were subdivided to control, 12 hours, 1 day, 2 days, 4 days, 7 days and 10 days groupes and all of three pancreatic ducts of chicken were ligated by surgical procedure and then the morphological changes were observed. In pancreatic islets, the vacuolation and invasion of connective tissue were occurred in all experimental groups and dissociation of pancreatic islets was detected in 4 days after pancreatic duct ligation and hold out to 10 days. The peak of the morphological changes in pancreatic islets was detected in 4 days after pancreatic duct ligation. In the results of immunohistochemical methods against glucagon, insulin, somatostatin and bovine pancreatic polypeptide(BPP), the number of immunoreactive pancreatic islets were decreased but the size increased with time, so the number of immunoreactive cells in each pancreatic islets were increased. Glucagon-immunoreactive cells were not changed but insulin-immunoreactive cells were decreased with time(p<0.05). BPP-immunoreactive cells were increased in 2 days after pancreatic duct ligation and then decreased with time(p<0.05). Somatostatin-immunoreactive cells were increased with time(p<0.05) in dark islets.

• Journal of Korean Physical Therapy Science
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• v.9 no.4
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• pp.53-61
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• 2002

This study was carried out to investigate the effects of electrical stimulation on the change oxytocin(OT) immunoreactive cells in the hypothalamus of the male SD rats. Fifteen healthy and normal male rats were divided into three groups, 5 SD rat in each group. The one group has been stimulated by electro-acupuncture(EA, 2Hz) for 30 min and the other group by EA for 1hr 30 min and control group has not been stimulated. The results were summarized as follows. OT immunoreactive cells were found in the Paraventricular nucleus (PVN), Supraoptic nucleus(SON) and Lateral Magnocellular Area(LMA). The number of OT immunoreactive cells of PVN were significantly increased after 30 min as compared with control group (p<0.05) and were also significantly increased after 1hr 30min than 30 min(p<0.05). The number of OT immunoreactive cells of SON were significantly increased after 30 min and 1hr 30 min(p<0.05) as compared with control group, but decreased after 1hr 30 min than 30 min. The number of immunoreactive hells of LMA significantly increased after 30 min(p<0.05) as compared with control group, but significantly decreased after 1hr 30 min than 30 min(p<0.05). These results show that OT is released into the hypothalamus in response to electrical stimulation.

• Animal cells and systems
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• v.3 no.3
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• pp.269-273
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• 1999

The distribution and relative frequency of somatostatin-immunoreactive cells in the pancreas were studied during developmental stages (fetus, neonate, 1-month-old, 6-month-old and adult) of the Korean native goat by immunohistochemical methods. Somatostatin-immunoreactive cells were detected in the exocrine of all ages, in the endocrine portions (pancreatic islets) from the neonate, and in the pancreatic duct of the 1-month-old. The relative frequencies of these cells in the pancreatic islets increased with age. However, there were no age-related changes in the relative frequencies of somatostatin-immunoreactive cells in the exocrine and pancreatic duct. Generally, they were distributed in the interacinar spaces, the epithelium of the pancreatic duct, or dispersed in the peripheral zone of the pancreatic islets in all ages. However, clusters consisting of 3-4 cells were also found in the subepithelial connective tissues from the 1-month-old. In addition, the distributions in the endocrine portions of the adult were divided into two patterns: 1) they are dispersed in the marginal regions with moderate or low frequencies, or 2) in the inner zone with high frequencies.

• Korean Journal of Veterinary Research
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• v.38 no.2
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• pp.232-238
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• 1998

The distributions and relative frequencies of bovine chromogranin(BCG)-, serotonin-, and bovine pancreatic polypeptide(BPP)-immunoreactive cells in the pancreas of the duck(Anas plaryrhynchos plaryrhyncos, Linne) were studied immunohistochemically on 23 days of incubation, at hatching, 1 week, 2 weeks, 3 weeks, 5 weeks, 6 weeks, 7 weeks, 9 weeks, 10 weeks, and 32 weeks after hatching. In the exocrine portions, the relative frequencies of BCG- and serotonin-immunoreactive cells tend to decrease according to ages. BPP-immunoreactive cells first appeared 1 week after hatching, and thereafter decreased. These cells were mainly observed in the exocrine pancreas and marginal region of the pancreatic islets. In the endocrine portions, BCG-, serotonin- and BPP-immunoreacteve cells disappeared 2 weeks, 9 weeks and 5 weeks after hatching, respectively.

• Korean Journal of Veterinary Research
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• v.38 no.4
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• pp.696-703
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• 1998

Histological changes and ontogeny, distribution and relative frequency of bovine SP-1/chromogranin(BCG)-, serotonin-, human pancreatic polypeptide(HPP)- and glucagon-immunoreactive cells were investigated in the colo-rectum of the chicken embryos. The pseudostratified columnar-like epithelium was observed in 10 days of incubation to 15 days of incubation, thereafter these epithelium were differentiated to simple columnar epithelium and goblet cells were detected for the first time on 19 days of incubation. BCG and serotonin-immunoreactive cells were observed for the first time on 15 days of incubation respectively, thereafter these cells were increased with ages. However no HPP and glucagon-immunoreactive cells were found in this study.