• 제목/요약/키워드: immuno activity

검색결과 235건 처리시간 0.032초

목질진흙 (상황)버섯의 면역활성 (Immuno-stimulating Activity of Phellinus linteus)

  • 송치현;나경수;양병근;전용재
    • 한국균학회지
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    • 제26권1호통권84호
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    • pp.86-90
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    • 1998
  • 상황버섯 자실체, 액체배양 균사체 및 배양액으로부터 고분자물질(polymer)을 추출하여 면역활성을 anti-complementary assay 방법으로 측정하였다. 자연산과 인공재배 자실체로부터 생산된 polymer의 면역활성은 각각 65.77%와 63.94%로 나타나 큰 차이를 보이지 않았다. 자실체, 균사체 및 배양액으로부터 추출한 polymer의 면역활성 측정결과, 균사체는 41.95%, 배양액은 21.87%로 자실체 보다 낮게 나타났다.

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해수 미세조류인 Chlorella capsulata의 면역활성증진 (Screening of the Immuno-stimulatory Activity of the Marine Alga Chlorella capsulate)

  • 이현수;이서호;문형철;이현용
    • KSBB Journal
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    • 제18권1호
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    • pp.19-24
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    • 2003
  • 해수 미세조류인 Chlorella capsulata를 배양하여 그로부터 기능성물질을 분리하여 면역활성측정 및 항암실험을 실시하였다. C. capsulata를 이용한 면역활성능 측정실험에서 일반적 인 담수종의 추출물이나 같은 종의 여러 추출용매를 이용한 추출물보다 물추출물의 분획층에 대한 면역활성이 높은 것으로 나타났다. 인간 T 세포, B 세포와 대식세포에 대해서 높은 면역활성을 나타내었으며, 정상세포에 대한 독성측정 실험에서도 약 10∼25%의 독성을 나타났으며, 유방암세포와 위암세포에 대한 성장저해 실험에서도 각각 80% 이상의 높은 저해능을 확인하였다. 앞서의 결과들로부터 Chloreila capsulata로부터 분리한 분획물은 면역세포들의 생육을 증강시켜 외부항원에 대한 면역기능을 강화시키는 것을 간접적으로 확인할 수 있었다

한의학의 항종양 면역치료에 관한 연구 -1990년 이후 발표된 실험논문을 중심으로- (Compilation of 104 Experimental Theses on the Antitumor and Immuno-activating therapies of Oriental Medicine)

  • 강연이;김태임;박종오;김성훈;박종대;김동희
    • 동의생리병리학회지
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    • 제17권1호
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    • pp.1-24
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    • 2003
  • This study was done to compile 104 experimental theses which are related to the antitumor and immuno-activating therapies between February 1990 through February 2002. Master's and doctoral theses were dassified by schools, degrees, materials, effects, experimental methods of antitumor and immunoactivity, and results. The following results were obtained from this study : 1. Classifying the theses by the school, 34.6% were presented by Daejeon University, 29.8% by Kyung-hee University and 11.5% by Won-kwang University. Of all theses, 51.0% were aimed for the doctoral degree and 43.3% were for the master's degree. All of three universities have their own cancer centers. 2. Classifying the theses by herb materials, complex prescription accounted for 60.3%, single herb accounted for 24.8% and herbal acupuncture accounted for 14.2%. Considering the key principles of the traditional medicine, complex prescription was much more thoroughly studied than single herb prescription. The results showed that the complex prescription had both antitumor activity and immuno-activating activity, which might reflects on multi-activation mechanisms by complex components. 3. Classifying the theses by the efficacy of herbs examined, in single herb, invigorating spleen and supplementing was 35.5%, expelling toxin and cooling was 29.0%, activating blood flow and removing blood stasis was 12.9%. In herbal acupuncture, invigorating spleen and supplementing was 52.9%, expelling toxin and cooling was 29.4%. In complex prescription, pathogen-free status was 41.9%, strengthening healthy qi to eliminate pathogen was 35.5%, strengthening healthy qi was 22.6%. It is presumed that the antitumor and immunoactivating therapy based on syndrome differentiation is the best way to develop oriental oncology. 4. Classifying the theses by antitumor experiments, cytotoxic effect was 48.1 %, survival time was 48.1 % and change of tumor size was 42.3%. Survival rate was not necessarily correlated with cytotoxicity. These data reflect the characteristic, wholistic nature of the oriental medicine which is based on BRM (biological response modifier). 5. Classifying the theses by immunoactivating experiments, hemolysin titer was 51.0%, hemagglutinin titer was 46.2% and NK cell's activity was 44.2%. In the future studies, an effort to elucidate specific molecular and cellular mechanisms of cytokine production in the body would be crucial. 6. Classifying the theses according to the data in terms of antitumor activity, 50% was evaluated good, 24.0% was excellent, and 15.5% have no effect. In an evaluation of immuno-activating activity, 35.9% was excellent and 18.0% showed a little effect. The index point, as described here, may helps to use experimental data for clinical trials. Changes in index points by varying dosage implicate the importance of oriental medical theory for prescription. 7. In 167 materials, IIP (immunoactivating index point, mean : 3.12±0.07) was significantly higher than AIP(antitumor index point, mean : 2.83±0.07). These data demonstrate that the effect of herb medicine on tumor activity depends more on immunoactivating activity than antitumor activity. This further implies that the development of herbal antitumor drugs must be preceded by the mechanistic understanding of immunoactivating effect. 8. After medline-searching tumor and herb-related articles from NCBI web site, we conclude that most of the studies are primarily focused on biomolecular mechanisms and/or pathways. Henceforth, we need to define the biomolecular mechanisms and/or pathways affected by herbs or complicated prescriptions. 9. Therefore, the most important point of oriental medical oncology is to conned between experimental results and clinical trials. For the public application of herbal therapy to cancer, it is critical to present the data to mass media. 10. To develop the relationship of experimental results and clinical trials, university's cancer clinic must have a long-range plan related to the university laboratories and, at the same time, a regular consortium for this relationship is imperative. 11. After all these efforts, a new type herbal medicine for cancer therapy which is to take care of the long-term administering and safety problem must be developed. Then, it would be expected that anti-tumor herbal acupuncture can improve clinical symptoms and quality of life (QOL) for cancer patients. 12. Finally, oriental medical cancer center must be constructed in NCC (National Cancer Center) or government agency for the development of oriental medical oncology which has international competitive power.

감잎에서 분리한 다당의 면역자극 활성 (Immuno-stimulating Activities of Polysaccharide Fractions Isolated from Persimmon Leaves)

  • 신영아;박혜령;홍희도;신광순
    • 한국식품영양학회지
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    • 제25권4호
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    • pp.941-950
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    • 2012
  • In order to develop new physiologically active polysaccharides from persimmon leaves, two different crude polysaccharides were prepared using hot water (PLW-0) and pectinase digestion (PLE-0) and their immuno-stimulating activities were estimated. PLW-0 and PLE-0 showed similar sugar compositions with 15 different sugars, including rarely observed sugars in general polysaccharides such as 2-O-methyl-fucose, 2-O-methyl-xylose, apiose, aceric acid, 3-deoxy-D-manno-2-octulosonic acid, and 3-deoxy-D-lyxo-2-heptulosaric acid, but the uronic acid content of PLE-0 was lower than that of PLW-0 caused by pectinase treatment. Both PLW-0 and PLE-0 showed potent anti-complementary activity in a dose-dependent manner which was similar to a known immuno-stimulating polysaccharide, PSK, from Coriolus versicolor. The activity of PLE-0 at a low concentration ($100{\mu}g/m{\ell}$) was higher than that of PLW-0. In an in vitro cytotoxicity analysis, PLW-0 and PLE-0 (up to $1,000{\mu}g/m{\ell}$) did not affect the growth of peritoneal macrophages and Colon 26-M3.1 carcinoma cells. In contrast, they enhanced lymphocyte proliferation activity. Peritoneal macrophages stimulated with PLW-0 and PLE-0 produced various cytokines, such as IL-6 and IL-12. However, PLE-0 was more effective on the cytokine production. Intravenous administration of PLW-0 and PLE-0 significantly augmented natural killer (NK) cell cytotoxicity against Yac-1 tumor cells 3 days after the treatment of polysaccharide fractions. But NK cells obtained from the PLE-treated group showed higher tumoricidal activity even at a low dose of $40{\mu}g$/mouse. In experimental lung metastasis of Colon 26-M3.1 carcinoma cells, prophylactic administration of PLW-0 and PLE-0 significantly inhibited lung metastasis in a dose-dependent manner and PLE-0 was more effective on the inhibition of cancer metasasis. The results lead us to conclude that the pectinase-treated process is indispensable to preparing polysaccharides with higher immune-stimulating activity from persimmon leaves.

복분자 안토시아닌 분획의 항산화, 항암 및 면역증진 효과 (Antioxidant, Anticancer and Immune Activation of Anthocyanin Fraction from Rubus coreanus Miquel fruits (Bokbunja))

  • 정명근;임정대
    • 한국약용작물학회지
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    • 제20권4호
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    • pp.259-269
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    • 2012
  • This study was performed to determined the antioxidant activities, anticancer and immuno-activities of anthocyanin fraction from Rubus coreanus Miquel fruits (Bokbunja). Anthocyanin fraction extracted from Bokbunja revealed the presence of three anthocyanin components, which were tentatively identified as cyanidin 3-O-sambubioside, cyanidin 3-O-xylosylrutinoside and cyanidin 3-O-rutinoside using RP-HPLC/DAD/MS. The anthocyanin fraction from Bokbunja always showed reducing power and high scavenging activities against DPPH, hydroxy radical (OH) and superoxide anion radical ($O_2{^-}$) similar to general synthetic antioxidant and polyphenol compounds from plant origin. Anthocyanin fraction from Bokbunja showed high inhibition on proliferation of LNCaP and A549 cells and did not inhibit the proliferation of other cancer cells. Immuno-activities of Anthocyanin fraction from Bokbunja were investigated, it showed high promotion of human B and T cells growth about 50% and secretion of IL-6 and TNF-${\alpha}$ by treatment after 6 days. Over all, the result of the study suggest that anthocyanin fraction from Bokbunja displays antioxidant activity comparable to that general synthetic antioxidant, also, anthocyanin fraction from Bokbunja are expected to be good candidate for development into source of anticaner and immuno-activator agent in food industry.

Radiochemical separation of 89Zr: a promising radiolabel for immuno-PET

  • Vyas, Chirag K.;Park, Jeong Hoon;Yang, Seung Dae
    • 대한방사성의약품학회지
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    • 제2권1호
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    • pp.43-50
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    • 2016
  • $^{89}Zr$ with the favorable nuclear decay kinetics and chemical properties is an appealing radiometal for its application in immuno-PET using radiolabeled monoclonal antibodies. Rising demand of ultrahigh purity and high-specific activity $^{89}Zr$ has propelled the radiochemist worldwide to develop an overall efficacious method for its promising separation from the target matrix $^{89}Y$. The requirement of elevated radiochemical purity (${\geq}$ 99.99%) has accelerated the efforts since last two decades to achieve higher decontamination and separation factors of carrier free $^{89}Zr$ over $^{89}Y$ using several suitable separation techniques. However, each of the technique has its own pros and cons which prior to its actual medical application needs to be optimized and thoroughly scrutinized to avoid further complications during radiolabelling of the pharmaceuticals. In this short review article we will specifically consider as well focus on the historical development and the recent advances on the radiochemical separation of $^{89}Zr$ from $^{89}Y$ which will be helpful for the separation scientist involved in this area to understand the existing available means and plan the strategy to investigate and develop the novel techniques to overcome the problems involved in the present methods.

Ochnaflavone의 면역보조제 효과 (Effect of Ochnaflavone as An Immunoadjuvant)

  • 박민주;유기연;한용문
    • 약학회지
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    • 제56권6호
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    • pp.366-371
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    • 2012
  • In this present study, we determined whether or not there is an immunoadjuvant effect of ochnaflavone, a biflavone isolated from Lonicera japonica. As an antigenic source, the cell wall (CACW) of Candida albicans, a fungal pathogen, was used. CACW consists of 95% carbohydrate (mannan). In the experiments, BALB/c mice were immunized with emersion forms of CACW combined with or without ochnaflavone (Och) in the presence of IFA containing mineral oil or CACW alone. Then, the amounts of antisera collected from these mice groups were measured by the ELISA method. Data from these experiments showed that CACW combined with Och (CACW/Och/IFA) provoked the production of antisera app. 2.2 or 5 times more than the corresponding CACW/IFA or CACW alone (CACW/DPBS), respectively, in mice (P<0.05). We further examined the immune response type induced by Och. Analysis of the values of the IgG1/IgG2a ratios obtained from IgG isotyping revealed that Och induced Th2-immunity more dominantly than Th1. This finding was confirmed by cytokine profile. CACW/Och/IFA formulation induced IL-4 (Th2-type cytokine) more than IFN${\gamma}$ (Th1-type cytokine) as compared with CACW/IFA and CACW/DPBS formulations (P<0.05). All data combined, Och appears to have an immunoadjuvant activity that may convert Th1 immunity into Th2 immunity.

감식초에서 분리한 면역활성다당의 특성 (Characterization of Immuno-stimulating Polysaccharides Isolated from Korean Persimmon Vinegar)

  • 황용철;신광순
    • 한국식품과학회지
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    • 제40권2호
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    • pp.220-227
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    • 2008
  • 한국 전통발효음료 중에 존재하는 특이다당류의 화학적 특성 및 생물활성을 규명할 목적으로, 농가에서 직접 발효한 감식초로부터 다당류를 분리하고 이들의 각종 면역증강활성에 대해 검토하였다. 감식초에 80% ethanol 침전을 행하여 얻어진 조다당 획분 PV-0을 이용, 연속적인 3회의 column chromatography를 행하여, 감식초의 주요 다당인 PV-1b-I으로 정제할 수 있었다. PV-1b-I획분은 HPLC상에서 대칭을 유지하는 단일 peak로 검출되었으며, 분자량은 약 110 kDa으로 평가되었다. 정제다당인 PV-1b-I의구성당 조성을 확인한 결과, mannose(46.8%), galactose(28.5%) 및 arabinose(19.1%)를 높은 비율로 함유하고 있었다. 또한 본 정제다당은 ${\beta}$-glucosyl Yariv reagent와 강하게 반응하는 특성을 보여주었으며 이러한 사실로부터 PV-1b-I이 arabino-3,6-galactan 부위를 소유함을 확인할 수 있었다. 한편 감식초에서 정제한 다당 PV-1b-I은 in vitro상에서 macrophage의 활성화를 높은 비율로 유도하였으며, 비장세포에 대해 높은 증식능을 보였다. 또한 PV-1b-I을 정맥 투여한 경우, NK cell을 활성화하여 YAC-1 종양세포에 대한 세포독성을 증가시킴이 관찰되었다. 한편 고분자 정제 다당인 PV-1b-I는 비특이적 면역계에 있어 중요 역할을 담당하고 있는 보체계에 대하여 농도 의존적인 활성화 경향을 보였다. 이들은 $Ca^{2+}$ 이온이 제거된 상태에서의 항보체 활성과 anti-human C3를 이용한 2차원 면역전기영동에 의하여 C3 산물을 동정한 결과로부터 보체계의 classical pathway와 alternative pathway 양 경로를 모두 경유하여 활성을 나타냄을 확인할 수 있었다.

효모변이주 Saccharomyces cerevisiae IS2 세포벽 유래의 베타글루칸 면역활성능에 관한 연구 (Study on Immuno-stimulating Activity of ${\beta}$-Glucan Isolated from the Cell Wall of Yeast Mutant Saccharomyces cerevisiae IS2)

  • 박정훈;강만식;김홍일;정봉현;이광호;문원국
    • 한국식품과학회지
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    • 제35권3호
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    • pp.488-492
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    • 2003
  • S. cereviaiae KCTC 7911에 돌연변이를 유도하고 selective pressure로서 세포벽 분해효소인 zymolsae와 mechanical stress인 glass bead를 차례로 처리하여 효모변이주를 S. cerevisiae IS2를 선발하였다. S. cerevisiae IS2는 세포벽 분해효소인 zymolase의 농도별 내성실험 결과 wild-type에 비해 훨씬 강한 내성을 보여 세포벽에 변화가 일어난 균주로 예상된다. 효모변이주와 wild-type으로부터 베타글루칸을 추출하여 면역활성에 미치는 영향을 조사하기 위해 생쥐의 복강에 주사하고 생성되는 면역세포의 수, NO 생성능, 및 면역세포의 대다수를 차지하는 대식세포의 탐식능을 측정하였다. 베타글루칸을 쥐의 복강에 주사하였을 때 베타글루칸의 종류에 상관없이 면역세포의 수, NO 생성능 및 대식세포의 활성도가 증가하는 결과를 얻을 수 있었다. 특히 변이주 베타글루칸을 주사하였을 경우 wild-type 베타글루칸에 비해 면역세포의 수는 1.40배, NO 생성능은 1.12배, 대식세포의 활성도와 탐식능은 각각 1.18배와 1.43배 높은 수치를 얻을 수 있었다. 이러한 결과들로 미루어 변이주 베타글루칸이 wild-type 베타글루칸보다 우수한 면역활성 촉진능력을 가지고 있음을 증명할 수 있었으며, 고부가가치 기능성 면역물질로서의 응용 가능성을 확인할 수 있었다.

Effect of Dietary Lipid Sources on Growth, Enzyme Activities and Immuno-hematological Parameters in Catla catla Fingerlings

  • Priya, K.;Pal, A.K.;Sahu, N.P.;Mukherjee, S.C.
    • Asian-Australasian Journal of Animal Sciences
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    • 제18권11호
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    • pp.1609-1616
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    • 2005
  • Ninety advanced Catla catla fingerlings (av. wt. 16 g) were randomly distributed in six treatment groups with three replicates each for an experimental period of 60 days to study the effect of dietary lipid source on growth, enzyme activities and immuno-hematological parameters. Six isoprotein (40.0-41.9%) and isocaloric (4,260 kcal $kg^{-1}$) semi-purified diets were prepared with varying levels of soybean oil (SBO) and cod liver oil (CLO) within a total of 8% lipid viz., $D_1$ (Control), $D_2$ (8% SBO), $D_3$ (6% SBO and 2% CLO), $D_4$ (4% SBO and 4% CLO), $D_5$ (2% SBO and 6% CLO) and $D_6$ (8% CLO). Highest SGR was noted in $D_5$ (0.73${\pm}$0.03) group, which was similar with $D_3$ (0.71${\pm}$0.02) and $D_4$ (0.69${\pm}$0.01) groups. Activity of intestinal lipase, hepatic glucose-6-phosphate dehydrogenase (G6PDH) and aspartate amino transferase (AST) of the lipid treatment groups were significantly higher (p<0.05) than the control group. The respiratory burst activity of the phagocytes (Nitroblue tetrazolium (NBT)) was highest in $D_2$ (1.95${\pm}$0.21) followed by $D_3$ (1.19${\pm}$0.15) group, which were significantly (p<0.05) higher than the other groups. Globulin level was significantly higher in $D_3$ (1.29${\pm}$0.08) than in the other groups expect $D_4$. Hemoglobin content and total erythrocyte count did not show any significant difference. From this study, it is concluded that a diet containing 6% soybean oil and 2% cod liver oil ($D_3$) yields higher growth and immune response in Catla catla fingerlings and would be cost effective.