• 전자공학회논문지
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• 제49권10호
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• pp.194-201
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• 2012

뜸은 질병에 대한 면역력을 높이고 질병을 치유하는데 효과적인 반면 뜸의 열 자극 세기를 조절하기 힘들어 피부에 고통을 유발시키며 화상을 입히는 부작용이 발생된다. 이런 한계점을 극복하기 위해 자극 조절이 가능한 고주파 열 자극 시스템을 개발하였으며, 동물에게 뜸질을 할 경우 얻어지는 온도분포와 유사한 자극 프로토콜을 개발하였다. 자극을 위한 시스템은 2MHz, 200W의 고주파유전가열장치, 절연프로브, 절연플레이트, 음압 발생부 및 온도측정부로 구성되어있다. 자극 프로토콜 설계를 위해서 일차적으로 토끼의 대퇴부에 뜸질시 표피, 피하 5mm와 피하10mm에서 온도분포를 획득하였다. 획득된 뜸질의 온도분포와 유사한 온도분포로 자극하기 위해서 자극펄스의 duty ratio와 반복회수 및 출력을 제어하였다. 뜸질과 고주파 자극시 피하의 온도분포를 비교해본 결과 피하 5mm와 10mm 부위에서의 상관관계는 각각 95%와 91%로 일치함을 보여주었다. 또한, 고주파 자극시 표피에서의 온도분포는 뜸에 비해 현저하게 낮게 나옴을 확인하였다. 이를 통해 고주파 열 자극 시스템은 뜸질의 문제점인 화농, 물집과 같은 부작용을 유발시키지 않으면서 피하에 뜸질과 유사한 열 자극이 가능함을 보여주었다.

• Journal of Nutrition and Health
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• 제34권3호
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• pp.271-284
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• 2001

This study was performed to investigate the effects of dried powders and water extracts of Paecilomyces tenuipes(P. tenuipes) and Cordyceps militaris(C. militaris) on lipid metabolism, lipid peroxidation and antioxidative capacity and immune status in rats. Thirty-five male Sprague-Dawley rats weighting 195$\pm$21g were grouped into five according to body weight. Ratw were raised for four weeks with diet containing either 4%, 2%(w/w) of dried P. tenuipes powders(TP-4, TP-2) or water extracts from equal amounts of each 4% P. tenuipes and C. militaris powder(TE-4, ME-4). Food intake, weight gain of all groups were not significantly different from those of control group. Lipid metabolism in general was not significantly different among all the groups. However both dried P. tenuipes powder lowered plasma cholesterol level slightly, water extract groups showed tendency of higher plasma HDL-cholesterol and lower liver cholesterol levels than control. Plasma and liver thiobarbituric acid reactive substance(TBARS) concentrations of all the experimental groups were lower than control group. Red blood cell(RBC) and liver superoxide dismutase(SOD) activities were not generally different among all groups. Liver xanthine oxidase(XOD) activities of all groups were tended to be lower than control group. Proliferation of aplenocytes induced by mitogens, concanavalin A and lipopolysaccharide, were increased in TP-2 group. The TP-4 group showed increased CD8 T cells and MHC class II expression without changes in CD4 T cells, B cells and G/M ratio, suggesting activated cytotoxic T cell activity in vivo. Increase of G/M ratio but not of MHC class II in TP-2 group indicated the possible acute inflammatory reaction by the ingested substances in gastrointestinal tract. ME-4 group showed enhanced cellular immunity without vigorous changes of immune parameters in brief periods. In conclusion, both P. tenuipes and C. militaris stimulated antioxidant capacity and immune status in rats. Among groups, water extract of C. militaris was most effective in both capacities, though dried powder of P. tenuipes at 2% dietary level was more effective in antioxidant activity, as various results by different strains were observed.(Korean J Nutrition 34(3) : 271~284, 2001)

• Journal of Microbiology and Biotechnology
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• 제14권4호
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• pp.722-729
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• 2004

Leptin is an adipocyte-secreted hormone and its plasma levels correlate with total body fat mass, however, it also plays a regulatory role in immunity, inflammation, and hematopoiesis. Chemokine is known as a chemoattractant cytokine in inflammatory reaction, but its role in leptin reaction has not been well studied. In this study, the direct effect of leptin on the expression of chemokine mRNAs and lipopolysaccharide (LPS)-induced chemokine KC mRNA in mouse peritoneal macrophages was investigated. Leptin did not induce the expression of lymphotactin, RANTES, eotaxin, MIP-1$\beta$, MIP-1$\alpha$, MIP-2, MCP-1, IP-10, TCA-3, and KC mRNA in mouse peritoneal macrophages, and had no direct effect on the expression of these LPS-induced chemokine mRNAs except KC mRNA. The synergistic effect of leptin on the expression of LPS-induced KC mRNA occurred late in the time course of response to LPS. The increased expressions of Ob-Rb mRNA and leptin receptor protein were detected during the LPS treatment. Leptin produced a substantial increase in the stability of the LPS-induced KC mRNA, and the synergistic effect of leptin on LPS-induced KC mRNA expression was further augmented by cycloheximide (CHX). Pyrrolidine dithiocarbamate (PDTC) did not block the synergistic effect of leptin on LPS-induced KC mRNA expression in mouse peritoneal macrophages. These data suggest that although leptin has no direct effect on the expression of lymphotactin, RANTES, eotaxin, MIP-1$\beta$, MIP-1$\alpha$, MIP-2, MCP-1, IP-10, TCA-3, and KC mRNA in mouse peritoneal macrophages, the synergistic effect of leptin on the expression of LPS-induced KC mRNA has the possibility that LPS might induce the expression of the Ob-Rb receptor or an unknown gene(s) that sensitizes macrophages to the synergistic function of leptin. Therefore, further studies are necessary to examine leptin as a regulatory factor of chemokine production.

• 한국식품과학회지
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• 제47권4호
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• pp.511-516
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• 2015

발아 전후 보리 당단백질 추출물에 대한 영양성분 변화와 RAW 264.7 세포에 처리 후 생성하는 산화질소 II와 사이토카인(인터류킨-6, 종양괴사인자-${\alpha}$) 양을 측정하여 면역 증진 효능을 예측하였다. 보리의 발아가 진행됨에 따라 당단백질 추출물의 단백질과 지방질 함량은 감소하고 총당 함량은 증가하는 추세를 확인하였다. 주요 중성당은 아라비노스, 포도당, 자일로스이며 발아함에 따라 6탄당인 포도당은 감소한 반면, 5탄당인 아라비노스와 자일로스는 증가하였다. 아미노산 함량은 GEB에 비해 24-GEGB와 48-GEGB에서 각각 1.03, 1.24배 증가하였다. 면역 활성을 측정하기 위하여 RAW 264.7 세포에 24-GEGB와 48-GEGB를 처리한 결과 GEB에 비해 더 높은 산화질소 II와 사이토카인(인터류킨-6, 종양괴사인자-${\alpha}$)을 생성하였다. 결론적으로, 보리 종자가 발아하면 종자 내 단백질, 지방질 및 당질 등의 영양성분 조성이 변하고, 그 결과 발아보리 당단백질 추출물은 외부 침입에 대해 1차적으로 방어하는 대식세포를 자극하여 세포에 독성을 미치지 않는 범위의 면역매개물질 생성하여 면역 증진 효과를 기대할 수 있는 것으로 판단된다.

• 한국양식학회지
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• 제22권1호
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• pp.56-62
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• 2009

Effects of Korean mistletoe extracts (KM-110), Viscum album coloratum on the specific and non-specific immune responses of Japanese eel Anguilla japonica were examined. The optimal concentration not showing toxicity of KM-110 was determined to $30-40{\mu}g/ml$ in vitro and $100{\mu}g$/100 g of fish in vivo. Even $1000{\mu}g$ of KM-110/100 g of fish did not show any clinical problem in fish though the levels of toxic parameters were slightly increased. In terms of antibody production, KM-110 significantly elicited more antibody production than FCA or $\beta$-glucan. $\beta$-glucan plus KM-110 group synergistically enhanced antibody production. There was no significant difference between KM-110 and KM-110 plus $\beta$-glucan group. The ROI production by head kidney (HK) leucocytes of eel injected with 500 or $1000{\mu}g$ KM-110 was significantly (P<0.05) enhanced than the control and FCA-treated group. Maximum increase in the NBT reduction value was observed in $1000{\mu}g$ KM-110 group but no significant difference was found between 500 and $1000{\mu}g$ KM group. The level of serum lysozyme activity was significantly (P<0.05) higher in the 500 and $1000{\mu}g$ KM-110- or FCA-treated group than in the control and $200{\mu}g$ KM-110 group. The phagocytic activities of HK leucocytes isolated from eel injected with 500 and $1000{\mu}g$ KM-110 were significantly (P<0.05) higher than $200{\mu}g$ KM-110 and PBS-injected control group. Korean mistletoe appeared to be a good activator of the specific and non-specific immune responses of Japanese eel.

• 수산해양교육연구
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• 제28권6호
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• pp.1715-1723
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• 2016

복합 생약재 열수추출물(6종 혼합)을 농도별 기간별로 사료에 흡습시켜 12주 동안 넙치에 급여 후 비특이적 면역 및 질병저항성에 대한 효과를 조사하였다. 복합 생약재 열수추출물의 첨가는 어류 성장에는 아무런 영향을 미치지 않았으며 혈액생화학적인, 결과도 넙치의 생리에는 아무런 영향을 미치지 않았다. 라이소자임 활성은 0.01%의 복합 생약재 열수추출물 농도로 흡습시킨 사료 그룹에서 라이소자임 활성이 증가됨을 알 수 있었다. 복합 생약재 열수추출물의 투여에 따른 넙치 장기의 병리조직에 영향이 없음을 알 수 있었다. 에드와드균의 인위감염에 의한 질병저항성은 0.01% 복합 생약재 열수추출물 농도에서 상대생존율이 높게 나타났다. 이상의 결과를 종합하여 볼 때 넙치에 복합 생약재 열수추출물을 투여시에는 어류의 비특이적 면역력 증강 및 질병 저항성 증강을 나타내는 것으로 사료되었다.

• Corrosion Science and Technology
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• 제9권6호
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• pp.276-280
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• 2010

Copper(Cu) as an interconnecting metal layer can replace aluminum (Al) in IC fabrication since Cu has low electrical resistivity, showing high immunity to electromigration compared to Al. However, it is very difficult for copper to be patterned by the dry etching processes. The chemical mechanical polishing (CMP) process has been introduced and widely used as the mainstream patterning technique for Cu in the fabrication of deep submicron integrated circuits in light of its capability to reduce surface roughness. But this process leaves a large amount of residues on the wafer surface, which must be removed by the post-CMP cleaning processes. Copper corrosion is one of the critical issues for the copper metallization process. Thus, in order to understand the copper corrosion problems in post-CMP cleaning solutions and study the effects of DC biases and post-CMP cleaning solution concentrations on the Cu film, a constant voltage was supplied at various concentrations, and then the output currents were measured and recorded with time. Most of the cases, the current was steadily decreased (i.e. resistance was increased by the oxidation). In the lowest concentration case only, the current was steadily increased with the scarce fluctuations. The higher the constant supplied DC voltage values, the higher the initial output current and the saturated current values. However the time to be taken for it to be saturated was almost the same for all the DC supplied voltage values. It was indicated that the oxide formation was not dependent on the supplied voltage values and 1 V was more than enough to form the oxide. With applied voltages lower than 3 V combined with any concentration, the perforation through the oxide film rarely took place due to the insufficient driving force (voltage) and the copper oxidation ceased. However, with the voltage higher than 3 V, the copper ions were started to diffuse out through the oxide film and thus made pores to be formed on the oxide surface, causing the current to increase and a part of the exposed copper film inside the pores gets back to be oxidized and the rest of it was remained without any further oxidation, causing the current back to decrease a little bit. With increasing the applied DC bias value, the shorter time to be taken for copper ions to be diffused out through the copper oxide film. From the discussions above, it could be concluded that the oxide film was formed and grown by the copper ion diffusion first and then the reaction with any oxidant in the post-CMP cleaning solution.

• 한국콘텐츠학회논문지
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• 제19권3호
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• pp.500-509
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• 2019

본 연구는 체질푸드가 간호사의 자연치유력증진에 있어서 스트레스 감소와 NK세포 활성도에 미치는 영향에 대해 밝히고자 하였으며, 이를 통해 체질푸드가 인간의 건강 예방과 치유에 효과적인지를 고찰하였다. 연구는 경기도 한 종합병원에서 현재 1년 이상 근무 경력이 있는 간호사 22명을 대상으로 30일 동안 진행되었다. 본 연구에서는 스트레스 측정을 위해 유비오맥파측정기(uBioClip v70)를 사용하였고 NK세포 활성도에 대해서는 혈액검사를 통해 진행하였다. 수집된 자료처리는 통계분석 프로그램인 SPSS 21을 통해 대응표본 t검증으로 진행하였으며, 황제내경과 장상학에 근거한 체질분류 후 참여자의 체질에 알맞은 체질 푸드를 제공하여 실시하였다. 연구 결과, 체질푸드의 스트레스 감소에 미치는 영향은 유의한 정(+)의 효과를 나타내었고, NK세포 활성도에 대해서 체질푸드가 효과적인 것으로 나타났다. 본 연구는 의의는 다음과 같다. 첫째, 현대인의 건강을 위협하는 주 요소인 스트레스에 대해 체질푸드를 통한 자연치유력 강화가 스트레스에 대한 저항력을 높여줌으로써 질병에 대한 예방 효과가 있다는 점이다. 둘째, 질병의 예방과 치료에 있어서 면역세포 활성도는 매우 중요한데, 체질푸드를 통한 NK세포 활성도를 높여줌으로써 인체의 자연치유력을 증대시킬 수 있다는 점이다.

• 한국환경생태학회지
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• 제33권3호
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• pp.333-340
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• 2019

다문화 배경 학생들이 늘어남에 따라 이들의 교육에 대한 관심이 높아지고 있다. 본 연구에서는 숲이 가지고 있는 산림치유인자들과 요인들을 활용하여 다문화 배경 학생들의 언어습득향상과 자아탄력성에 미치는 영향을 알아보고자 하였다. 실험대상은 청주시 ${\bigcirc}{\bigcirc}$동에 위치한 다문화예비학교 중학생 남녀 각각 10명을 대상으로 방과 후에 산림치유프로그램을 운영하였다. 실험기간은 2018년 4월12일부터 2018년 6월26일까지 매주 1회씩 12회기로 1시간 (60분)씩 실시하였다. 산림치유프로그램은 숲에 존재하는 다양한 환경요소를 활용하여 인체의 면역력을 높이고 신체적 정신적 건강을 회복시키는 활동이다. 프로그램 실시 전과 후에 자아탄력성의 차이를 알아보기 위해 대응표본 t-test를 실시하고 그 자료는 SPSS 18.0 프로그램으로 분석하였다. 그 결과 자아탄력성은 긍정사고능력, 문제해결능력, 친밀행동능력, 감정조절능력, 자율행동능력 등 하위요인 모두에서 통계적으로 유의미하게 향상되었다(p<.001). 언어능력향상은 기술통계로 알아본 결과 쓰기의 오류, 발음의 오류, 문장의 오류, 시제의 오류, 조사 접속사의 오류 모두에서 향상되었다. 본 연구결과가 향후 중도입국자녀들이나 다문화 배경 학생들의 자아탄력성과 언어습득향상에 기초자료로 활용되기를 기대한다.

• BMB Reports
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• 제52권4호
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• pp.289-294
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• 2019

The present study evaluated the role of AHNAK in Bartonella henselae infection. Mice were intraperitoneally inoculated with $2{\times}10^8$ colony-forming units of B. henselae Houston-1 on day 0 and subsequently on day 10. Blood and tissue samples of the mice were collected 8 days after the final B. henselae injection. B. henselae infection in the liver of Ahnak-knockout and wild-type mice was confirmed by performing polymerase chain reaction, with Bartonella adhesion A as a marker. The proportion of B. henselae-infected cells increased in the liver of the Ahnak-knockout mice. Granulomatous lesions, inflammatory cytokine levels, and liver enzyme levels were also higher in the liver of the Ahnak-knockout mice than in the liver of the wild-type mice, indicating that Ahnak deletion accelerated B. henselae infection. The proportion of CD4+interferon-${\gamma}$ ($IFN-{\gamma}^+$) and $CD4^+$ interleukin $(IL)-4^+$ cells was significantly lower in the B. henselae-infected Ahnak-knockout mice than in the B. henselae-infected wild-type mice. In vitro stimulation with B. henselae significantly increased $IFN-{\gamma}$ and IL-4 secretion in the splenocytes obtained from the B. henselae-infected wild-type mice, but did not increase $IFN-{\gamma}$ and IL-4 secretion in the splenocytes obtained from the B. henselae-infected Ahnak-KO mice. In contrast, $IL-1{\alpha}$, $IL-1{\beta}$, IL-6, IL-10, RANTES, and tumor necrosis $factor-{\alpha}$ secretion was significantly elevated in the splenocytes obtained from both B. henselae-infected wild-type and Ahnak-knockout mice. These results indicate that Ahnak deletion promotes B. henselae infection. Impaired $IFN-{\gamma}$ and IL-4 secretion in the Ahnak-knockout mice suggests the impairment of Th1 and Th2 immunity in these mice.