• Journal of Microbiology and Biotechnology
• /
• v.29 no.1
• /
• pp.105-113
• /
• 2019

Although siderophore compounds are mainly biosynthesized as a response to iron deficiency in the environment, they also bind with other metals. A few studies have been conducted on the impact of heavy metals on the siderophore-mediated iron uptake by microbiome. Here, we investigated siderophore production by a variety of rhizosphere fungi under different concentrations of $Zn^{2+}$ ion. These strains were specifically isolated from the rhizosphere of Panax ginseng (Korean ginseng). The siderophore production of isolated fungi was investigated with chrome azurol S (CAS) assay liquid media amended with different concentrations of $Zn^{2+}$ (50 to $250{\mu}g/ml$). The percentage of siderophore units was quantified using the ultra-violet (UV) irradiation method. The results indicated that high concentrations of $Zn^{2+}$ ion increase the production of siderophore in iron-limited cultures. Maximum siderophore production by the fungal strains was detected at $Zn^{2+}$ ion concentration of $150{\mu}g/ml$ except for Mortierella sp., which had the highest siderophore production at $200{\mu}g/ml$. One potent siderophore-producing strain (Penicillium sp. JJHO) was strongly influenced by the presence of $Zn^{2+}$ ions and showed high identity to P. commune (100% using 18S-rRNA sequencing). The purified siderophores of the Penicillium sp. JJHO strain were chemically identified using UV, Fourier-transform infrared spectroscopy (FTIR), and matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF-MS) spectra.

• Kyungpook Mathematical Journal
• /
• v.63 no.2
• /
• pp.235-250
• /
• 2023

Let 1 ≤ p, q < ∞ be fixed, and let R = [r_jk] be an infinite scalar matrix such that 1 ≤ r_jk < ∞ and sup_j,k r_jk < ∞. Let 𝓑(𝑙_p, 𝑙_q) be the set of all bounded linear operator from 𝑙_p into 𝑙_q. For a fixed Banach algebra 𝐁 with identity, we define a new vector space S^R_p,q(𝐁) of infinite matrices over 𝐁 and a paranorm G on S^R_p,q(𝐁) as follows: let $$S^R_{p,q}({\mathbf{B}})=\{A:A^{[R]}{\in}{\mathcal{B}}(l_p,l_q)\}$$ and $G(A)={\parallel}A^{[R]}{\parallel}^{\frac{1}{M}}_{p,q}$, where $A^{[R]}=[{\parallel}a_{jk}{\parallel}^{r_{jk}}]$ and M = max{1, sup_j,k r_jk}. The existance of S^R_p,q(𝐁) equipped with the paranorm G(·) including its completeness are studied. We also provide characterizations of β -dual of the paranormed space.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.16 no.5
• /
• pp.1671-1686
• /
• 2022

Face manipulation tools represented by Deepfake have threatened the security of people's biological identity information. Particularly, manipulation tools with deep learning technology have brought great challenges to Deepfake detection. There are many solutions for Deepfake detection based on traditional machine learning and advanced deep learning. However, those solutions of detectors almost have problems of poor performance when evaluated on different quality datasets. In this paper, for the sake of making high-quality Deepfake datasets, we provide a preprocessing method based on the image pixel matrix feature to eliminate similar images and the residual channel attention network (RCAN) to resize the scale of images. Significantly, we also describe a Deepfake detector named Cascaded-Hop which is based on the PixelHop++ system and the successive subspace learning (SSL) model. By feeding the preprocessed datasets, Cascaded-Hop achieves a good classification result on different manipulation types and multiple quality datasets. According to the experiment on FaceForensics++ and Celeb-DF, the AUC (area under curve) results of our proposed methods are comparable to the state-of-the-art models.

• Advances in Traditional Medicine
• /
• v.1 no.1
• /
• pp.8-27
• /
• 2000

Components of extracellular matrix and the matrix-degrading enzymes are some of the key regulators of tumor metastasis and angiogenesis. Hyaluronic acid (HA), a matrix glycosaminoglycan, is known to promote tumor adhesion and migration, and its small fragments are angiogenic. Until now, we have compared levels of hyaluronidase, an enzyme that degrade HA, in normal adult prostate, benign prostate hyperplasia and prostate cancer tissues and in conditioned media from epithelial explant cultures, using a substrate (HA)-gel assay and ELISA-like assay (Kim et al., unpublished results). The present review described an overall characterization of hyaluronidases and its application to human diseases. The hyaluronidases are a family of enzymes that have, until recently, deed thorough explication. The substrate for these enzymes, hyaluronan, is becoming increasingly important, recognized now as a major participant in basic processes such as cell motility, wound healing, embryogenesis, and implicated in cancer progression. And in those lower life forms that torment human beings, hyaluronidase is associated with mechanisms of entry and spread, e.g. as a virulence factor for bacteria, for tissue dissection in gas gangrene, as a means of treponema spread in syphilis, and for penetration of skin and gut by nematode parasites. Hyaluronidase also comprises a component of the venom of a wide variety of organisms, including bees, wasps, hornets, spiders, scorpions, sh, snakes and lizards. Of particular interest is the homology between some of these venom hyaluronidases and the enzyme found in the plasma membrane of mammalian spermatozoa, attesting to the ancient nature of the conserved sequence, a 36% identity in a 300 amino acid stretch of the enzyme protein. Clearly, hyaluronidase is of biological interest, being involved in the pathophysiology of so many important' human disorders. Greater effort should be made in studying this family of enzymes that have, until recently, been overlooked. Also, oriental medical application of the hyaluronidase will be discussed with respect to inhibition and suppression of inflammation and malignacy.

• Journal of the Computational Structural Engineering Institute of Korea
• /
• v.20 no.2
• /
• pp.113-125
• /
• 2007

Improved numerical method to obtain the exact stiffness matrices is newly proposed to perform the spatially coupled elastic and stability analyses of non-symmetric and open/closed thin-walled beam on elastic foundation. This method overcomes drawbacks of the previous method to evaluate the exact stiffness matrix for the spatially coupled stability analysis of thin-walled beam-column This numerical technique is accomplished via a generalized eigenproblem associated with 14 displacement parameters by transforming equilibrium equations to a set of first order simultaneous ordinary differential equations. Next polynomial expressions as trial solutions are assumed for displacement parameters corresponding to zero eigenvalues and the eigenmodes containing undetermined parameters equal to the number of zero eigenvalues are determined by invoking the identity condition. And then the exact displacement functions are constructed by combining eigensolutions and polynomial solutions corresponding to non-zero and zero eigenvalues, respectively. Consequently an exact stiffness matrix is evaluated by applying the member force-deformation relationships to these displacement functions. In order to illustrate the accuracy and the practical usefulness of this study, the numerical solutions are compared with results obtained from the thin-walled beam and shell elements.

• Asian-Australasian Journal of Animal Sciences
• /
• v.18 no.4
• /
• pp.483-489
• /
• 2005

To understand the molecular mechanisms that regulate intramuscular fat deposition and its release, cDNA clones expressed in adipose tissues of Korean cattle were identified by differential screening from adipose tissue cDNA library. By partial nucleotide sequencing of 486 clones and a search for sequence similarity in NCBI nucleotide databases, 245 clones revealed unique clones. By a functional grouping of the clones, 14% of the clones were categorized to metabolism and enzyme-related group (stearoyl CoA desaturase, lactate dehydrogenase, fatty acid synthase, ATP citrate lyase, lipoprotein lipase, acetyl CoA synthetase, etc), and 6% to signal transduction/cell cycle-related group (C/EBP, cAMP-regulated phosphoprotein, calmodulin, cyclin G1, cyclin H, etc), and 4% to cytoskeleton and extracellular matrix components (vimentin, ankyrin 2, gelosin, syntenin, talin, prefoldin 5). The obtained 245 clones will be useful to study lipid metabolism and signal transduction pathway in adipose tissues and to study obesity in human. Some clones were subjected to full-sequencing containing open reading frame. The cDNA clone of bovine homolog of human prefoldin 5 gene had a total length of 959 nucleotides coding for 139 amino acids. Comparison of the deduced amino acid sequences of bovine prefoldin 5 with those of human and mouse showed over 95% identity. The cDNA clone of bovine homolog of human ubiquitin-like/S30 ribosomal fusion protein gene had a total length of 484 nucleotides coding for 133 amino acids. Comparison of the deduced amino acid sequences of bovine ubiquitin-like/S30 ribosomal fusion protein gene with those of human, rat and mouse showed over 97% identity. The cDNA clone of bovine homolog of human proteolipid protein 2 mRNA had a total length of 928 nucleotides coding for 152 amino acids. Comparison of the deduced amino acid sequences of bovine proteolipid protein 2 with those of human and mouse showed 87.5% similarity. The cDNA clone of bovine homolog of rat thymosin beta 4 had a total length of 602 nucleotides coding for 44 amino acids. Comparison of the deduced amino acid sequences of bovine thymosin beta 4 gene with those of human, mouse and rat showed 93.1% similarity. The cDNA clone of bovine homolog of human myotrophin mRNA had a total length of 790 nucleotides coding for 118 amino acids. Comparison of the deduced amino acid sequences of bovine myotrophin gene with those of human, mouse and rat showed 83.9% similarity. The functional role of these clones in adipose tissues needs to be established.

• Journal of Korea Multimedia Society
• /
• v.13 no.3
• /
• pp.378-391
• /
• 2010

In this paper, it is tested that an individual is able to be identified with finger face images and the results are presented. Special operators, FFG(Facet Function Gradient) masks by which the gradient of a facet function fit on a gray levels of image patches can be computed are used and a new procedure named F-algorithm is introduced to match the finger face images. The finger face image is divided into the equal subregions and each subregions are divided into equal patches with this algorithm. The FFG masks are used for convolution operation over each patch to produce scalar values. These values from a feature matrix, and the identity of fingers is determined by a norm of the elements of the feature matrices. The distribution of the norms shows conspicuous differences between the pairs of hand images of the same persons and the pairs of the different persons. This is a result to prove the ability of discrimination with the finger face image. An identification rate of 95.0% is obtained as a result of the test in which 500 hand images taken from 100 persons are processed through F-algorithm. It is affirmed that the finger face reveals to be such a good biometrics as other hand parts owing to the ability of discrimination and the identification rate.

• Food Science and Biotechnology
• /
• v.17 no.5
• /
• pp.912-918
• /
• 2008

This study was conducted to identify the differences in proteomic characteristics of 'Agakong', recombinant inbred line, and its parental genotypes 'Eunhakong' (Glycine max) and 'KLG10084' (G. soja). The isoflavone content of 'Agakong' was 3 times higher than that of its parental lines. A combined high-throughput proteomic approach was employed to determine the expression profile and identity of proteins using 2-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. The overall distribution patterns of proteins are quite similar, but lots of protein spot intensities varied among the genotypes. A total of 41 proteins, representing significant difference in the quantities of protein among the lines, were successfully identified. Among them, more than 50% of the proteins identified were subunits of glycinin and $\beta$-conglycinin, 2 major storage proteins. This study showed that the proteomic analysis could help to define specific changes in protein level and composition, which can occur in the generation of new soybean varieties.

• Korean Journal of Food Science and Technology
• /
• v.43 no.1
• /
• pp.12-16
• /
• 2011

This study was conducted to identify the differences in proteomic characteristics between Ca-enriched king oyster mushrooms and general king oyster mushrooms. A combined high-throughput proteomic approach was employed to determine the expression profiles and identity of proteins using 2-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. The overall distribution patterns of the proteins were quite similar, but many of the protein spot intensities varied. A total of 10 proteins, representing a significant difference in the quantities of protein betweenthe two types of mushrooms, were successfully identified. Among these proteins, eight kinds were increased in the Ca-enriched king oyster mushrooms and two kinds were decreased. This study showed that proteomic analysis can help define specific changes in protein level and composition, which can occur in mushrooms where Ca content may or may not be enriched.

• Korean Journal of Health Education and Promotion
• /
• v.32 no.4
• /
• pp.67-78
• /
• 2015

Objectives: This study aims to examine the trends of mobile-health research in terms of community participation. Methods: A set of 24 peer-reviewed articles were identified for review. Two authors independently reviewed the articles using a literature review matrix and reexamined as a team. Review categories include: general characteristics, research methods, levels and ranges of community participation, and research topics. Results: Most of the articles were published in 2013-2014, including one domestic paper. Multidisciplinary approaches were used in 65% of the studies. Community participation was at low or mid-levels of Arnstein's participation model. In mobile-health research so far, the level of participation tends to improve as more diverse stakeholders participate in health promotion initiatives with mobile-health. The review yielded five types of mobile-health research for community health promotion: improving the quality of primary healthcare through the community health workers' capacity enhancement; improving the data collection capabilities; facilitating exchanges of community information and resources; reinforcing community identity; and monitoring physical environment of the communities. Conclusions: Although at an early stage of research development, application of mobile-health to community health promotion via participation has a potential. Multi-disciplinary approaches should be fostered for further development.