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Changes in oligosaccharide content during the storage period of maesil cheong formulated with functional oligosaccharides (기능성 올리고당으로 제조한 매실청의 저장기간 중 올리고당 함량 변화)

  • Bae, Moon-Joo;Yoo, Sang-Ho
    • Korean Journal of Food Science and Technology
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    • v.51 no.2
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    • pp.169-175
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    • 2019
  • This study was carried out to produce the health functional food maesil cheong by replacing sucrose with isomaltooligosaccharide and fructooligosaccharide. The substitution levels of these oligosaccharides were between 10% and 100%. A 1:1 (w/w) mixture of maesil and sugar was adopted for preparing maesil cheong. The pH of maesil cheong remained unchanged (between 2.72 and 3.00) during 90-day storage period, regardless of oligosaccharide content. Citric and malic acids were identified in maesil cheong; citric acid accounted for 71-82% of the total organic acid content. Sucrose was completely liquefied in the sample after 30 days and was hydrolyzed steadily into fructose and glucose over the storage period. More than 75% of isomaltooligosaccharides remained in maesil cheong after 90 days when sucrose was completely replaced with isomaltooligosaccharide. However, fructooligosaccharides were mostly decomposed at the end of storage period. Thus, isomaltooligosaccharides may be suitable for acidic maesil cheong products to expect its health functional effect.

A Study for establishment of soil moisture station in mountain terrain (1): the representative analysis of soil moisture for construction of Cosmic-ray verification system (산악 지형에서의 토양수분 관측소 구축을 위한 연구(1): Cosmic-ray 검증시스템 구축을 위한 토양수분량 대표성 분석 연구)

  • Kim, Kiyoung;Jung, Sungwon;Lee, Yeongil
    • Journal of Korea Water Resources Association
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    • v.52 no.1
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    • pp.51-60
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    • 2019
  • The major purpose of this study is to construct an in-situ soil moisture verification network employing Frequency Domain Reflectometry (FDR) sensors for Cosmic-ray soil moisture observation system operation as well as long-term field-scale soil moisture monitoring. The test bed of Cosmic-ray and FDR verification network system was established at the Sulma Catchment, in connection with the existing instrumentations for integrated data provision of various hydrologic variables. This test bed includes one Cosmic-ray Neutron Probe (CRNP) and ten FDR stations with four different measurement depths (10 cm, 20 cm, 30 cm, and 40 cm) at each station, and has been operating since July 2018. Furthermore, to assess the reliability of the in-situ verification network, the volumetric water content data measured by FDR sensors were compared to those calculated through the core sampling method. The evaluation results of FDR sensors- measured soil moisture against sampling method during the study period indicated a reasonable agreement, with average values of $bias=-0.03m^3/m^3$ and RMSE $0.03m^3/m^3$, revealing that this FDR network is adequate to provide long-term reliable field-scale soil moisture monitoring at Sulmacheon basin. In addition, soil moisture time series observed at all FDR stations during the study period generally respond well to the rainfall events; and at some locations, the characteristics of rainfall water intercepted by canopy were also identified. The Temporal Stability Analysis (TSA) was performed for all FDR stations located within the CRNP footprint at each measurement depth to determine the representative locations for field-average soil moisture at different soil profiles of the verification network. The TSA results showed that superior performances were obtained at FDR 5 for 10 cm depth, FDR 8 for 20 cm depth, FDR2 for 30 cm depth, and FDR1 for 40 cm depth, respectively; demonstrating that those aforementioned stations can be regarded as temporal stable locations to represent field mean soil moisture measurements at their corresponding measurement depths. Although the limit on study duration has been presented, the analysis results of this study can provide useful knowledge on soil moisture variability and stability at the test bed, as well as supporting the utilization of the Cosmic-ray observation system for long-term field-scale soil moisture monitoring.

Exploring the Role of Collaborative Reflection in Small Group Argumentation: Focus on Students' Epistemic Considerations and Practices (소집단 논변 활동에서 협력적 성찰의 역할 탐색 -학생들의 인식적 고려와 실행을 중심으로-)

  • Cho, Hanbit;Ha, Heesoo;Kim, Heui-Baik
    • Journal of The Korean Association For Science Education
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    • v.39 no.1
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    • pp.1-12
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    • 2019
  • This study aims to explore students' epistemic practices and considerations, which are explained as underlying epistemic thoughts that guide their epistemic practices, during argumentation in science classrooms. We also investigated how collaborative reflection facilitated the development of such epistemic considerations. Two seventh-grade classes participated in this study by engaging in argumentation activities and collaborative reflection after classes. A group with students' change in epistemic aspects and the influence of collaborative reflection clearly revealed from their practices was chosen as a focus group. We recorded their class discussions and collaborative reflections with the researchers. Transcriptions of the recordings and checklists we collected during the collaborative reflections were used for analysis. Results showed evident changes in the students' epistemic considerations and practices and four factors facilitating such developments were identified. First, the researcher facilitating the students to recognize each other as collaborators during collaborative reflection led development of epistemic considerations on "audience using the knowledge products." Second, the collaborative reflection facilitated construction of context for peer interactions where the students encouraged each other to participate in the discussion, resulting in the development of other students' epistemic considerations on "justifications in knowledge products." Third, the items provided on the checklists explicitly delineated expectations on their practices in argumentation, also facilitating development of epistemic considerations. Lastly, the students' imitation of the researcher's pattern of discourse facilitated construction of causal explanation and development of epistemic considerations on "nature of the knowledge products." This study will contribute to the construction of strategies that develop students' epistemic considerations and productive epistemic practices in argumentation.

Investigation of bacteria in the agricultural by-products imported for the use as media materials in mushroom cultivation (버섯재배 배지재료용 수입 농업부산물에서의 세균 조사 연구)

  • Kim, Jun Young;Kim, Susan;Kim, Seong Hwan
    • Korean Journal of Microbiology
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    • v.54 no.4
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    • pp.410-419
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    • 2018
  • It is urgently required to construct safety data on agricultural by-products imported for use as medium materials for domestic mushroom production. However, research on microorganisms is insufficient. This study was conducted to investigate the presence of bacteria that have the possibility of harmful effects on human, plants and mushroom in wheat straw, peatmoss, cottonseed hull, cottonseed meal, and beet pulp imported from Australia, Canada, China, Egypt, Germany. Bacteria were found in the range of $1.35{\times}10^2$ to $8.34{\times}10^6CFU/g$. As a result of 16S rDNA sequence analysis, total of 19 genera and 45 species of bacteria were identified. Bacillus genus was dominant, followed by Paenibacillus genus. At the species level, diverse species was in the order of Firmicute, Proteobacteria and Actinobacteria. Regarding the agricultural by-products, straw and peat moss had more diverse bacteria than other agricultural by-products. Among the indentified bacteria, 6 species of 5 genera (Enterobacter asburiae, Enterobacter ludwigii, Stenotrophomonas maltophilia, Pseudomonas monteilii, Bacillus anthracis, and Cellulosimicrobium funkei) were present as potent harmful bacteria to human. Surprisingly, both the human and plant pathogenic Klebsiella pneumoniae subsp. pneumonia was present. Bacillus altitudinis was present as a plant pathogen. Lysinibacillus sphaericus, an insect pathogen, and Ochrobactrum pseudogrignonense, a mushroom pathogen, were also present. The results of this study confirmed that several kinds of pathogenic bacteria were present in the agricultural by-products for the mushroom cultivation medium imported into Korea. Our work suggests that hygiene inspection and management is urgently needed for imported agricultural by-products to be safely used for mushroom production.

A Study on the Entrepreneurial Orientation and the Performance of Startups: The Mediating Effects of Technological Orientation and Social Capital (스타트업의 기업가지향성과 성과에 관한 연구: 기술지향성과 사회적 자본의 매개효과)

  • Lee, Eun A;Seo, Joung Hae;Shim, Yun Soo
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.14 no.2
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    • pp.47-59
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    • 2019
  • Various studies have been carried out on the subject of entrepreneurship, which is required to create new businesses and organizations during the early process of startups based on innovative technologies and ideas. At the same time, the concept of organizational entrepreneurial orientation, which explains how to manage enterprises in the process of pioneering new products and markets, is drawing more and more attention for the purpose of continuously creating and maintaining a competitive edge of startups. This study focused on the relationship between entrepreneurial orientation and startup performance and the role of technological orientation and social capital. An empirical research was conducted on 144 different startup companies residing in startup supporting institutions. To evaluate the suitability of the research model, a PLS-based structural equation model was used. The research results are as follows: First, the entrepreneurial orientation of startups was found to have a positive effect on startup performance. Second, it was shown that entrepreneurial orientation had a positive effect on all three dimensions of social capital and technological orientation. Third, it has been shown that technological orientation and the cognitive dimension of social capital mediates the relationship between entrepreneurial orientation and startup performance. Through this, it was confirmed that entrepreneurial orientation directly affects startup performance, and it even influences the growth of startups by increasing technological superiority and social capital which is inherent in the network. Also, the research identified the need for additional research on the relationship between the strengthening of technological orientation and strategical orientation in startups. This study is expected to expand the discussion about social capital in the field of startup related research by affirming the role and importance of the cognitive system embedded in the network as well as the connectivity of networks, which has been already emphasized in previous startup related studies. Finally, the results of this study were reflected to present new practical implications.

Identification of DNA Markers Related to Resistance to Herbicide Containing Mesotrione in Tongil Type Rice (통일형 벼에서 메소트리온계 제초제 저항성 연관 DNA marker 탐색)

  • Lee, Ji-Yoon;Cho, Jun-Hyeon;Lee, Jong-Hee;Cho, Su-Min;Kwon, Young-Ho;Park, Dong-Soo;Song, You-Chun;Ko, Jong-Min
    • Korean Journal of Breeding Science
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    • v.50 no.4
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    • pp.387-395
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    • 2018
  • This study was conducted to identify DNA markers related to resistance to herbicide containing mesotrione in Tongil type rice. Two Tongil type elite lines; Milyang154 and Suweon382, showed resistance to mesotrione, whereas the others were susceptible at 20 days after mesotrione application, and severe growth inhibition was observed in the remaining 13 lines. As a result of analysis of mesotrione resistance using 190 $F_2$ populations derived from a cross of Hanareum2 (susceptible) and Milyang154 (resistant), the mesotrione resistance locus was shown to be a single dominant gene with a 3:1 segregation ratio ($X^2=1.19$, P=0.31). To identify a DNA marker closely linked to the mesotrione resistance gene, bulked segregant analysis (BSA) was adopted. The DNA marker RM3501 was identified on chromosome 2 with a recombinant value of 0.53 to the mesotrione resistance gene. Mst1(t) was located between SSR (simple sequence repeat) markers RM3501 and RM324 with a physical map distance of 10.2 Mb-11.4 Mb on chromosome 2. The band pattern of agarose gel electrophoresis of the SSR marker RM3501 showed the same segregation pattern with respect to mesotrione treatment in 20 Tongil type varieties and a $BC_2F_2$ segregation population derived from a cross between Unkwang (resistant) and Hanareum2 (susceptible). Thus, the RM3501 DNA marker could be used in breeding programs for Marker Assisted Selection in mesotrione resistant rice breeding.

The identification of non-synonymous SNP in the Enoyl-CoA delta isomerase 2 (ECI2) gene and its Association with Meat Quality Traits in Berkshire pigs (버크셔 돼지 육질 형질과 Enoyl-CoA delta isomerase 2 (ECI2) 유전자 nsSNP의 연관성 분석)

  • Hwang, Jung Hye;An, Sang Mi;Park, Da Hye;Kang, Deok Gyeong;Kim, Tae Wan;Park, Hwa Chun;Ha, Jeongim;Kim, Chul Wook
    • Journal of the Korean Society of International Agriculture
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    • v.30 no.4
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    • pp.277-284
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    • 2018
  • Meat quality has always been one of the most important factors that controls the choice of pork consumers and is of great interest in the pig industry. In this study, we identified a single nucleotide polymorphism (SNP) in the enoyl-CoA delta isomerase 2 (ECI2) gene in Berkshire pigs (n = 430) by analyzed the association between the SNP and meat quality traits. The non-synonymous SNP in the ECI2 gene is located at c.608 C > G and resulted in an amino acid change from threonine to serine. Significant associations between the SNP and meat quality traits, such as redness (CIE a), and the $pH_{24hr}$, were revealed in both the dominant and co-dominant models, whereas carcass weight, drip loss, and fat content and moisture content were significantly associated only with the dominant model. In barrow, the SNP was significantly associated with CIE a, drip loss, and $pH_{24hr}$, whereas in gilt, only a significant relationship with moisture content was observed. GG genotype pigs had a higher $pH_{24hr}$ and lower drip loss. Because $pH_{24hr}$ and drip loss are considered the most important meat quality traits, GG genotype pork is high-quality meat compared to that from other genotypes. In conclusion, the SNP in the ECI2 gene is significantly associated with several meat quality traits. These traits and could be as genetic markers in molecular breeding programs for improving meat quality.

Stage Structure and Population Persistence of Cypripedium japonicum Thunb., a Rare and Endangered Plants (희귀 및 멸종위기식물인 광릉요강꽃의 개체군 구조 및 지속성)

  • Lee, Dong-hyoung;Kim, So-dam;Kim, Hwi-min;Moon, Ae-Ra;Kim, Sang-Yong;Park, Byung-Bae;Son, Sung-won
    • Korean Journal of Environment and Ecology
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    • v.35 no.5
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    • pp.548-557
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    • 2021
  • Cypripedium japonicum Thunb. is an endemic plant in East Asia, distributed only in Korea, China, and Japan. At the global level, the IUCN Red List evaluates it as "Endangered Species (EN)," and at the national level in Korea, it is evaluated as "Critically Endangered Species (CR)." In this study, we investigated the characteristics of the age structure and the sustainability of the population based on the data obtained by demographic monitoring conducted for seven years in the natural habitat. C. japonicum habitats were observed in 7 regions of Korea (Pochoen, Gapyeong, Hwacheon, Chuncheon, Yeongdong, Muju, Gwangyang), and 4,356 individuals in 15 subpopulations were identified. The population size and structure differed from region to region, and artificial management had a very important effect on the size and structural change of the population. Population viability analysis (PVA) based on changes in the number of individuals of C. japonicum showed a very diverse tendency by region. And the probability of population extinction in the next 100 years was 0.00% for Pocheon, 10.90% for Gwangyang, 24.05% for Chuncheon, and 79.50% for Hwacheon. Since the above monitored study sites were located within the conservation shelters, which restricted access by humans, unauthorized collection of C. japonicum, the biggest threat to the species, was not reflected in the individual viability. So, the risk of extinction in Korea is expected to be significantly higher than that estimated in this study. Therefore, it is necessary to reflect population information in several regions that may represent various threats to determine the extinction risk of the C. japonicum population objectively. In the future, we should expand the demographic monitoring of the C. japonicum population known in Korea.

Predicting Suitable Restoration Areas for Warm-Temperate Evergreen Broad-Leaved Forests of the Islands of Jeollanamdo (전라남도 섬 지역의 난온대 상록활엽수림 복원을 위한 적합지 예측)

  • Sung, Chan Yong;Kang, Hyun-Mi;Park, Seok-Gon
    • Korean Journal of Environment and Ecology
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    • v.35 no.5
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    • pp.558-568
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    • 2021
  • Poor supervision and tourism activities have resulted in forest degradation in islands in Korea. Since the southern coastal region of the Korean peninsula was originally dominated by warm-temperate evergreen broad-leaved forests, it is desirable to restore forests in this region to their original vegetation. In this study, we identified suitable areas to be restored as evergreen broad-leaved forests by analyzing the environmental factors of existing evergreen broad-leaved forests in the islands of Jeollanam-do. We classified forest lands in the study area into six vegetation types from Sentinel-2 satellite images using a deep learning algorithm and analyzed the tolerance ranges of existing evergreen broad-leaved forests by measuring the locational, topographic, and climatic attributes of the classified vegetation types. Results showed that evergreen broad-leaved forests were distributed more in areas with a high altitudes and steep slope, where human intervention was relatively low. The human intervention has led to a higher distribution of evergreen broad-leaved forests in areas with lower annual average temperature, which was an unexpected but understandable result because an area with higher altitude has a lower temperature. Of the environmental factors, latitude and average temperature in the coldest month (January) were relatively less contaminated by the effects of human intervention, thus enabling the identification of suitable restoration areas of the evergreen broad-leaved forests. The tolerance range analysis of evergreen broad-leaved forests showed that they mainly grew in areas south of the latitude of 34.7° and a monthly average temperature of 1.7℃ or higher in the coldest month. Therefore, we predicted the areas meeting these criteria to be suitable for restoring evergreen broad-leaved forests. The suitable areas cover 614.5 km2, which occupies 59.0% of the total forest lands on the islands of Jeollanamdo, and 73% of actual forests that exclude agricultural and other non-restorable forest lands. The findings of this study can help forest managers prepare a restoration plan and budget for island forests.

Dynamic Changes of Urban Spatial Structure in Seoul: Focusing on a Relative Office Price Gradient (오피스 가격경사계수를 이용한 서울시 도시공간구조 변화 분석)

  • Ryu, Kang Min;Song, Ki Wook
    • Land and Housing Review
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    • v.12 no.3
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    • pp.11-26
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    • 2021
  • With the increasing demand for office space, there have been questions on how office rent distribution produces a change in the urban spatial structure in Seoul. The purpose of this paper is to investigate a relative price gradient and to present a time-series model that can quantitatively explain the dynamic changes in the urban spatial structure. The analysis was dealt with office rent above 3,306 m2 for the past 10 years from 1Q 2010 to 4Q 2019 within Seoul. A modified repeat sales model was employed. The main findings are briefly summarized as follows. First, according to the estimates of the office price gradient in the three major urban centers of Seoul, the CBD remained at a certain level with little change, while those in the GBD and the YBD continued to increase. This result reveals that the urban form of Seoul has shifted from monocentric to polycentric. This shows that the spatial distribution of companies has gradually accelerated decentralized concentration implying that the business networks have become significant. Second, contrary to small and medium-sized office buildings that have undertaken no change in the gradient, large office buildings have seen an increase in the gradient. The relative price gradients in small and medium-sized buildings were inversely proportional among the CBD, the GBD, and the YBD, implying their heterogeneous submarkets by office rent movements. Presumably, those differences in the submarkets were attributed to investment attraction, industrial competition, and the credit and preference of tenants. The findings are consistent with the hierarchical system identified in the Seoul 2030 Plan as well as the literature about Seoul's urban form. This research claims that the proposed method, based on the modified repeat sales model, is useful in understanding temporal dynamic changes. Moreover, the findings can provide implications for urban growth strategies under rapidly changing market conditions.