• Journal of Korea Water Resources Association
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• v.47 no.8
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• pp.671-684
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• 2014

Change point analysis is a efficient tool to understand the fundamental information in hydro-meteorological data such as rainfall, discharge, temperature etc. Especially, this fundamental information to change points to future rainfall data identified by reasonable detection skills can affect the prediction of flood and drought occurrence because well detected change points provide a key to resolve the non-stationary or inhomogeneous problem by climate change. Therefore, in this study, the comparative study to assess the performance of the 3 change point detection skills, cumulative sum (CUSUM) method, Bayesian change point (BCP) method, and segmentation by dynamic programming (DP) was performed. After assessment of the performance of the proposed detection skills using the 3 types of the synthetic series, the 2 reasonable detection skills were applied to the observed and future rainfall data at the 5 rainfall gauges in South Korea. Finally, it was suggested that BCP (with 0.9 posterior probability) could be best detection skill and DP could be reasonably recommended through the comparative study. Also it was suggested that BCP (with 0.9 posterior probability) and DP detection skills to find some change points could be reasonable at the North-eastern part in South Korea. In future, the results in this study can be efficiently used to resolve the non-stationary problems in hydrological modeling considering inhomogeneity or nonstationarity.

• Journal of Sericultural and Entomological Science
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• v.51 no.2
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• pp.137-141
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• 2013

In this study, nucleotide sequence structures of intergenic transcribed spacer (ITS) 1, complete 5.8S ribosomal RNA gene and ITS 2 region were analyzed to identify three Peruvian entomopathogenic fungal isolates. The isolates had highly conserved sequence region in 5.8S rRNA gene and unique sequences in ITS 1 and 2 region among them. 5.8S rRNA gene regions were highly conserved and showed high homoloies among tested isolates. In contrast, ITS region showed species-specific sequence region, resulting in inter-genus differencies. Scanning electron microscopic images of these isolates supported the result of ITS-based identification. From these result, Peruvian entomopathogenic fungal isolate J270, J278, were identified as Beauveria bassiana and J271 was identified as Lecanicillium attenuatum.

• Korean Journal of Plant Resources
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• v.23 no.5
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• pp.480-485
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• 2010

This study reports on the morphological characteristics and distribution for conservation and practical use in the Korean Peninsula of Chrysosplenium flaviflorum through a comparison with relative species. The morphological characters that were newly investigated in this study included subhypogaeus stolons and glabrous on the surface of leaves. The former has not been described by any taxonomist who had previously treated this species with the exclusion of Hara. Despite this, the latter is a very important key to using the identification of varieties of Ser. Pilosa though there were no descriptions which included Ohwi. C. flaviflorum Ohwi which was endemic and known to be distributed only in the northern area of the Korean Peninsula, was also observed in the Gapyeong-gun, Gyeonggi-do, Cheorwon-gun, Gangwon-do, and Yeongdonggun, Chungcheongbuk-do middle area since the latest collection report in 1943. There is a requirement for further study to be conducted on the distribution and management by designation for the Special Plant Species by the Korean Ministry of Environment.

• Molecules and Cells
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• v.37 no.7
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• pp.547-553
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• 2014

Glioblastoma multiforme (GBM) is one of the most common brain malignancies and has a very poor prognosis. Recent evidence suggests that the presence of cancer stem cells (CSC) in GBM and the rare CSC subpopulation that is resistant to chemotherapy may be responsible for the treatment failure and unfavorable prognosis of GBM. A garlic-derived compound, Z-ajoene, has shown a range of biological activities, including anti-proliferative effects on several cancers. Here, we demonstrated for the first time that Z-ajoene specifically inhibits the growth of the GBM CSC population. CSC sphere-forming inhibition was achieved at a concentration that did not exhibit a cytotoxic effect in regular cell culture conditions. The specificity of this inhibitory effect on the CSC population was confirmed by detecting CSC cell surface marker CD133 expression and biochemical marker ALDH activity. In addition, stem cell-related mRNA profiling and real-time PCR revealed the differential expression of CSC-specific genes, including Notch, Wnt, and Hedgehog, upon treatment with Z-ajoene. A proteomic approach, i.e., reverse-phase protein array (RPPA) and Western blot analysis, showed decreased SMAD4, p-AKT, 14.3.3 and FOXO3A expression. The protein interaction map (http://string-db.org/) of the identified molecules suggested that the AKT, ERK/p38 and $TGF{\beta}$ signaling pathways are key mediators of Z-ajoene's action, which affects the transcriptional network that includes FOXO3A. These biological and bioinformatic analyses collectively demonstrate that Z-ajoene is a potential candidate for the treatment of GBM by specifically targeting GBM CSCs. We also show how this systemic approach strengthens the identification of new therapeutic agents that target CSCs.

• Journal of Oriental Neuropsychiatry
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• v.21 no.2
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• pp.157-169
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• 2010

Objectives : The aim of this study was to investigate the characteristics of Hwa-Byung(HB) patients, draw the clinical key issue of HB, and provide the data as the basis for development of Clinical Guideline of HB. Methods : The study participants included 151 subjects who thought they have HB in 9 site. For all patients, we used HB epidemiologic study protocol, which include the Korean version of the Structured Clinical Interview for the DSM-IV Diagnosis (SCID-1), diagnostic criteria of HB, symptoms check list, pattern identification tool, psychiastric psychological questionaires, and Framingham coronary risk score(FRS). Results : 1. For all participants, 62 % of subjects were diagnosed with HB. These patients had comorbid DSM-IV diagnoses with depression (65%) and anxiety (27%). But 22% of these patients had only HB. 2. HB patients had various physical symptoms when visiting, were diagnosed as various disease, and undergone medical treatments in the past. Among the diagonsed illness, Gastronitestinal diseases (51.6%), psychiatric disorders(40.9%), endocrine diseases(39.8%) were being the most frequent. The frequent physical symptoms of HB patients reported were chest discomfort, head ache, pallpitation, frequent sigh, amnesia, shoulder pain, dry mouth, eye fatigue etc. 3. HB patients had high scores in psychologic questionares, CES-D, STAI, and STAXI. It means that HB patients might be low-level emotional stability. 4. Participant had negative opinion about the treatment of HB that it would be difficult or impossible (65.7%), but they neglect the need of medical treatment. Conclusions : This result shows that HB is comorbid with various psychiatric disease, but it has different physio-psychological symptoms from others and there were only HB patients. so we identify HB is independent disease. Moreover, there were the wrong perception of HB which blocking treatments. These problems support that the neccesity of development of Clinical Guideline of HB.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2010.05a
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• pp.14-14
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• 2010

Plastid proteomics are essential organelles present in virtually all cells in plants and green algae. Plastids are responsible for the synthesis and storage of key molecules required for the basic architecture and functions of plant cells. The proteome of plastid, and in particular of chloroplast, have received significant amounts of attention in recent years. Various fractionation and mass spectrometry (MS) techniques have been applied to catalogue the chloroplast proteome and its sub-organelles compartments. To better understanding the function of the lumenal sub-organelles within the thylakoid network, we have carried out a systematical analysis and identification of the lumenal proteins in the thylakoid of wheat by using Tricine-SDS-PAGE, and LTQ-ESI-FTICR mass spectrometry followed by SWISS-PROT database searching. We isolation and fractionation these membrane from fully developed wheat leaves using a combination of differential and gradient centrifugation couple to high speed ultra-centrifuge. After collecting all proteins to eliminate possible same proteins, we estimated that there are 407 different proteins including chloroplast, chloroplast stroma, lumenal, and thylakoid membrane proteins excluding 20 proteins, which were identified in nucleus, cytoplasm and mitochondria. A combination of these three programs (PSORT, TargetP, TMHMM, and TOPPRED) was found to provide a useful tool for evaluating chloroplast localization, transit peptide, transmembranes, and also could reveal possible alternative processing sites and dual targeting. Finally, we report also sub-cellular location specific protein interaction network using Cytoscape software, which provides further insight into the biochemical pathways of photosynthesis. The present work helps understanding photosynthesis process in wheat at the molecular level and provides a new overview of the biochemical machinery of the thylakoid in wheat.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2010.05a
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• pp.17-17
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• 2010

Ethylene, known as a stress hormone regulate wide developmental processes including germination, root hair initiation, root and shoot primordial formation and elongation, leaf and flower senescence and abscission, fruit ripening. The acceleration of ethylene biosynthesis in plant associated with environmental and biological stresses. 1-Aminocycloprophane-1-carboxlyate deaminase(ACCD) is an enzyme that cleaves ACC into and ammonia, a precursor of the plant hormone ethylene. Plant growth-promoting rhizobacteria (PGPR) having ACCD can decrease endogenous ACC level of tissue, resulting in reduced production of ethylene in plants. ACC deaminse was a key enzyme for protect stressed plants from injurious effects of ethylene. ACCD gene was encoded from Pseudomonas flourescens, PGPR and was cloned in Escherichia coli. We expressed the recombinant ACCD(rACCD) containing 357 amino acids with molecular weight 39 kDa that revealed by SDS-PAGE and western blot. The rACCD was purified by Ni-NTA purification system. The active form of rACCD having enzyme activity converted ACC to a-ketobutyrate. The optimal pH for ACC deaminase activity was pH 8.5, but no activity below pH 7.0 and a less severe tapering activity at base condition resulting in loss of activity at over pH 11. The optimal temperature of the enzyme was $30^{\circ}$ and a slightly less severe tapering activity at 15 - 30$^{\circ}$, but no activity over $35^{\circ}$. P. flourescens ACC deaminase has a highly conserved residue that plays in allowing substrate accessibility to the active sites. The enzymatic properties of this rACCD will provide an important reference for analysis of newly isolated ACCD and identification of newly isolated PGPR containing ACCD.

• Journal of Internet Computing and Services
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• v.14 no.4
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• pp.25-33
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• 2013

The procedure of the arbitration which is the tag collision is essential because the multiple tags response simultaneously in the same frequency to the request of the Reader. This procedure is known as Anti-collision and it is a key technology in the RFID system. In this paper, we propose the 4-Bit Pattern Slot Allocation(4-BPSA) algorithm for the high-speed identification of the multiple tags. The proposed algorithm is based on the tree algorithm using the time slot and identify the tag quickly and efficiently through accurate prediction using the a slot as a 4-bit pattern according to the slot allocation scheme. Through mathematical performance analysis, We proved that the 4-BPSA is an O(n) algorithm by analyzing the worst-case time complexity and the performance of the 4-BPSA is improved compared to existing algorithms. In addition, we verified that the 4-BPSA is performed the average 0.7 times the query per the Tag through MATLAB simulation experiments with performance evaluation of the algorithm and the 4-BPSA ensure stable performance regardless of the number of the tags.

• Korean Journal of Soil Science and Fertilizer
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• v.33 no.4
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• pp.275-282
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• 2000

Among the fluorescent pseudomonad isolates from soil- root system of red pepper in Chinju, Kyunsangnam-Do, the phylogenetic analysis for 35 isolates were conducted. The partial 16S ribosomal DNA sequences were used as taxonomic key for phylogenetic analyses, and these sequences were enabled to identification of the fluorescent pseudomonad isolates on the species level. The 17 isolates among them were classified into Pseudomonas putida group, and consisted of the strains isolated mainly from soil. This group were subdivided into 4 subgroups (I, II, III, and IV). The subgroup I and IV were unique ones which were relatively remotely related with subgroup II and III including the type strain of P. putida. The 15 isolates among 35 isolates were grouped along with the type strain of Pseudomonas fluorescens, and 3 isolate were characterized as intermediates of P. fluorescens and Pseudomonas chlororaphis. Most of strain isolateds from the rhizosphere soil and rhizoplane of red pepper were identified as P. fluorescens and closely related with each other. In this study, root of red pepper was supposed to be colonized by a specific strain or strains of P. fluorescens.

• Journal of the Korean Institute of Telematics and Electronics S
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• v.36S no.2
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• pp.69-80
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• 1999

Recently, social requirements of personal identification techniques are rapidly expanding in a number of new application ares. Especially fingerprint recognition is the most important technology. Fingerprint recognition technologies are well established, proven, cost and legally accepted. Therefore, it has more spot lighted among the any other biometrics technologies. In this paper we propose a new on-line fingerprint recognition algorithm for non-inked type live scanner to fit their increasing of security level under the computing environment. Fingerprint recognition system consists of two distinct structural blocks: feature extraction and feature matching. The main topic in this paper focuses on the feature matching using the fingerprint minutiae (ridge ending and bifurcation). Minutiae matching is composed in the alignment stage and matching stage. Success of optimizing the alignment stage is the key of real-time (on-line) fingerprint recognition. Proposed alignment algorithm using clique shows the strength in the search space optimization and partially incomplete image. We make our own database to get the generality. Using the traditional statistical discriminant analysis, 0.05% false acceptance rate (FAR) at 8.83% false rejection rate (FRR) in 1.55 second average matching speed on a Pentium system have been achieved. This makes it possible to construct high performance fingerprint recognition system.