• 제목/요약/키워드: identification experiment

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The Impact of Emotion on Focused Attention in a Flanker Task (수반자극과제에서 정서가 초점주의에 미치는 영향)

  • Park, Tae-Jin;Park, Sun-Hee
    • Korean Journal of Cognitive Science
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    • v.22 no.4
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    • pp.385-404
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    • 2011
  • We examined how emotional background stimuli influence focused attention in a flanker task. An IAPS picture was presented for 1,000ms in advance, then a target and two flanker letters were presented against the IAPS picture for 200ms(Experiment 1). The flanking stimuli were simultaneously presented on the left and right sides of the target stimulus with distance of $0.5^{\circ}C$, $1^{\circ}C$, or $1.5^{\circ}C$ visual angle. We investigated the flanker compatibility effect that identification of target would be faster when they were flanked by identical(compatible) stimuli than when they were flanked by different(incompatible) stimuli. Results of Experiment 1 revealed that the flanker compatibility effect depended not only on the distance of flankers but also on the valence of a background IAPS pictures. Positive and neutral pictures showed distance effect that the flanker compatibility effect was decreased as the farther the distance was, while negative pictures showed no di stance effect. Positive and neutral pictures showed compatibility effects at all distance conditions, but negative pictures didn't showed compatibility effect at $1.5^{\circ}C$ distance condition. In Experiment 2, the SOA(Stimulus Onset Asynchrony) between the picture and the stimuli of flanker task was manipulated. The flanking stimuli were presented simultaneously on the left and right sides of the target stimulus with a distance of either $0.5^{\circ}C$ or $1.5^{\circ}C$ visual angle. The results of Experiment 2 showed that flanker compatibility effect depends on SOA. At long SOA(2800ms), negative pictures showed no distance effect, but positive or neutral pictures did. All valence conditions of background pictures showed compatibility effects at $0.5^{\circ}C$ distance condition, but didn't showed compatibility effect at $1.5^{\circ}C$ distance condition. At short SOA(100ms), all valence conditions of background pictures showed distance effect, and showed compatibility effects with the exception of negative background pictures at $1.5^{\circ}C$ distance condition. These findings suggest that the scope of visual attention becomes narrower when viewing negative emotional stimuli and becomes broadened when viewing positive emotional stimuli. The narrowed scope of attention in negative emotion lasts longer, while the broaden scope of attention in positive emotion lasts shorter.

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A study on the soil conditioning behaviour according to mixing method in EPB shield TBM chamber (EPB 쉴드 TBM 챔버 내 혼합방법에 따른 배토상태거동에 대한 연구)

  • Kim, Yeon-Deok;Hwang, Beoung-Hyeon;Cho, Sung-Woo;Kim, Sang-Hwan
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.23 no.4
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    • pp.233-252
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    • 2021
  • This paper is a study to improve the efficiency of mixing technology in the shield TBM chamber. Currently, the number of construction cases using the TBM method is increasing in Korea. According to the increasing use of TBM method, research on TBM method such as Disc Cutter, Cutter bit, and Segment also shows an increasing trend. However, there is little research on the mixing efficiency in chamber and chamber. In order to improve the smooth soil treatment and the behavior of the excavated soil, a study was conducted on the change of the mixing efficiency according to the effective mixing bar arrangement in the chamber. In the scale model experiment, the ground was composed using plastic materials of different colors for ease of identification. In addition, the mixing bar arrangement was different and classified into 4 cases, and the particle size distribution was classified into single particle size and multiple particle size, and the experiment was conducted with a total of 8 cases. The rotation speed of the cutter head of all cases was the same as 5 RPM, and the experiment time was also carried out in the same condition, 1 minute and 30 seconds. In order to check the mixing efficiency, samples at the upper, middle (left or right), and lower positions of each case were collected and analyzed. As a result of the scaled-down model experiment, the mixing efficiency of Case 4 and Case 4-1 increased compared to Case 1 and Case 1-1, which are actually used. Accordingly, it is expected that the mixing efficiency can be increased by changing the arrangement of the mixing bar in the chamber, and it is considered to be effective in saving air as the mixing efficiency increases. Therefore, this study is considered to be an important indicator for the use of shield TBM in Korea.

Identification and classification of fresh lubricants and used engine oils by GC/MS and bayesian model (GC/MS 분석과 베이지안 분류 모형을 이용한 새 윤활유와 사용 엔진 오일의 동일성 추적과 분류)

  • Kim, Nam Yee;Nam, Geum Mun;Kim, Yuna;Lee, Dong-Kye;Park, Seh Youn;Lee, Kyoungjae;Lee, Jaeyong
    • Analytical Science and Technology
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    • v.27 no.1
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    • pp.41-59
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    • 2014
  • The aims of this work were the identification and the classification of fresh lubricants and used engine oils of vehicles for the application in forensic science field-80 kinds of fresh lubricants were purchased and 86 kinds of used engine oils were sampled from 24 kinds of diesel and gasoline vehicles with different driving conditions. The sample of lubricants and used engine oils were analyzed by GC/MS. The Bayesian model technique was developed for classification or identification. Both the wavelet fitting and the principal component analysis (PCA) techniques as a data dimension reduction were applied. In fresh lubricants classification, the rates of matching by Bayesian model technique with wavelet fitting and PCA were 97.5% and 96.7%, respectively. The Bayesian model technique with wavelet fitting was better to classify lubricants than it with PCA based on dimension reduction. And we selected the Bayesian model technique with wavelet fitting for classification of lubricants. The other experiment was the analysis of used engine oils which were collected from vehicles with the several mileage up to 5,000 km after replacing engine oil. The eighty six kinds of used engine oil sample with the mileage were collected. In vehicle classification (total 24 classes), the rate of matching by Bayesian model with wavelet fitting was 86.4%. However, in the vehicle's fuel type classification (whether it is gasoline vehicle or diesel vehicle, only total 2 classes), the rate of matching was 99.6%. In the used engine oil brands classification (total 6 classes), the rate of matching was 97.3%.

Molecular Genetic Classification of Phytophthora Species and P. infestans-specific Marker Selection by RAPD Fingerprinting (Phytophthora species의 분자유전학적 분류 및 RAPD fingerprinting을 이용한 P. infestans-specific 분자마커의 선발)

  • Kim, Kyoung-Su;Shin, Whan-Sung;Kim, Hee-Jong;Woo, Su-Jin;Ham, Young-Il;Shin, Kwan-Yong;Lee, Jeong-Oon;Kim, Byung-Sup;Shim, Jae-Ouk;Lee, Min-Woong;Lee, Youn-Su
    • The Korean Journal of Mycology
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    • v.27 no.6 s.93
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    • pp.394-398
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    • 1999
  • Taxonomic and genetic analysis of Phytophthora species belonging to six different morphological groups (GI, GII, GIII, GIV, GV, GVI) was conducted using RAPD method. Amplified fragments ranged $0.3{\sim}3.2$ kb in their molecular weights. Among total of 145 bands, there were 109 polymorphic bands. Seven isolates of P. infestans showed high similarities of $0.92{\sim}0.99$, and P. infestans isolate 3 from potato showed similarities of $0.93{\sim}0.95$ compared with other P. infestans. Among isolates of P. capsici, similarities of $0.77{\sim}0.86$ were observed and they were grouped in 80% level. P. cinnamomi and P. cryptogea isolates which belonging to group GVI showed very similar RAPD fingerprinting pattern. Primers OPA-04, OPA-17, OPA-18, OPA-19, and OPB-12 showed high level of differences among the tested isolates in major bands and molecular weights. The similarity between the isolates was 0.67. P. megasperma and P. sojae in group GV showed similarity of 0.65. These two isolates showed big differences in single major band in reactions with primers OPA-08, OPA-17, and OPA-19. Phytophthora-specific and P. infestans-specific molecular markers were also selected with one of the random primers tested. In reaction with primer OPA-20, all the genus Phytophthora showed common band at 600 bp, and all the P. infestans isolates showed specific band at 680 bp. These markers can be useful for identification of Phytophthora speices or P. infestans. As a result, P. infestans isolated from tomato and/or potato can easily be differentiated from other Phytophthora species with this primer.

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Studies on the Induction of Available Mutants of Takju Yeast by UV light Irradiation (Part 1) -On the Selection and Identification of the Mutants- (자외선조사(紫外線照射)에 의한 탁주효모(酵母)의 변이주육성(變異株育成)에 관한 연구(제 1 보) -변이주(變異株)의 선정(選定) 및 동정(同定)-)

  • Kim, Chan-Jo;Oh, Man-Jin;Kim, Seung-Yul
    • Applied Biological Chemistry
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    • v.18 no.1
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    • pp.10-15
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    • 1975
  • These studies were conducted to induce the available mutants in Takju yeasts by the irradiation of UV light. Two original strains(5-Y-5, 6-Y-6) using for irradiation of UV selected from 24 strains which were isolated from the Takju mashes And Nuruks collected from 12 local regions of Chungnam and Chungbuk provinces in Korea, and the irradiations to the yeasts with UV light were carried out at a distance 10-40cm from the sources of irradiation for 10-220 seconds. The purpose of this experiment is to report the effects of irradiating distances and times of UV light on the survival ratio of orginal yeasts, and the identification of two orginal yeasts and three mutants induced by the irradiation of UV light. The results were summarized as follows. 1) The effects of irradiating distances and times on the survival ratio on the yeasts were represented as follows. and acid productivity to the survival strains by the irradiation of UV light. The selected mutants were the strains 30-24, 40-27 which have more powerful fermentability about 10 percent than those of original strains and a strain 30-81 which have potential acid productivity. 3) The selected yeasts (5-Y-5, 6-Y-6) were identified to Saccharomyces cerevisiae by a taxonomic study of Lodder and the mutants(30-81, 40-27, 30-81) induced from above yeasts by the irradiation of UV light have almost same properties two orginal yeasts in the identical characteristics.

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Effects of the Applications of Clay Minerals on the Early Growth of Red Pepper in Growing Medium (점토광물 처리에 따른 상토에서 고추의 초기생장 효과)

  • Lee, Dong-Gi;Lee, Seok-Eon;Kim, Deok-Hyun;Hong, Hyeon-Ki;Nam, Ju-Hyun;Choi, Jong-Soon;Lee, Moon-Soon;Woo, Sun-Hee;Chung, Keun-Yook
    • Horticultural Science & Technology
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    • v.30 no.4
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    • pp.463-470
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    • 2012
  • As the advanced seeding technology through use of plug tray for good cultivation of seeds was propagated along with the expansion and development of horticultural industry, the use of bed soils as growing medium has recently been increased. In this study, the effects of the four clay minerals such as illite, phyllite, zeolite, and bentonite on the early growth of red pepper in the bed soil were investigated. Furthermore, proteome analysis for the leaf and stem samples of red pepper treated with only illite was performed. Of the seedling cultured, the healthy and regular size seeds were selected and cultivated in the pots, after they were treated with four clay minerals. The experiment was performed during the whole six weeks in the glasshouse of the Chungbuk National University. The growth lengths, fresh and dry weights of red pepper were significantly higher in the treatments of illite, phyllite, zeolite, and bentonite than in the control. In addition, the uptake of $K^+$, $Ca^{2+}$, and $Mg^{2+}$ were higher in the treatment of illite, phyllite, zeolite, and bentonite than in the control. The 2-DE patterns for the red pepper by the applications of illite, phyllite, zeolite, and bentonite were similar to each other. Therefore, compared to the samples of control, the proteome analysis for the samples of red pepper treated by only illite were performed. Proteome analysis for red pepper showed that plastid fructose-1, 6-bisphosphate aldolase class 1, aldolase, and glyceraldehydes 3-phosphate dehydrogenase, all of which were involved in the energy metabolism, were highly expressed in leaf tissue by illite treatment. In stem tissue, NAD-dependent formate dehydrogenase involved in energy metabolism, potassium transport protein, and GIA/RGA-like gibberellins response modulator were highly expressed. Based on the results obtained from the proteome analysis, it appears that the proteins specifically and differentially expressed on the illite treatment may be involved in the enhanced growth of red pepper. The identification of some proteins involved in the response of vegetable crops to the treatment of clay mineral can provide new insights that can lead to a better elucidation and understanding of mechanism on their molecular basis.

Isolation of Anti-inflammatory Active Substance β-Sitosterol from Seabuckthorn (Hippophae rhamnoides L.) Stem (비타민나무(Hippophae rhamnoides L.) 줄기로부터 항염 활성물질 β-Sitosterol의 분리)

  • Park, Yu-Hwa;Lim, Sang-Hyun;Ham, Hun-Ju;Kim, Hee-Yeon;Jeong, Haet-Nim;Kim, Kyung-Hee;Kim, Song-Mun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.7
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    • pp.980-985
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    • 2010
  • The objective of this study was to isolate and identify anti-inflammatory chemicals in Hippophae rhamnoides L. which was grown in Chuncheon, Korea. Treatment of ethanol extracts from stems, leaves, roots, and fruits to RAW 264.7 cells reduced amounts of nitrite by 56.0, 31.9, 49.1, and 18.9% respectively, compared to only lipopolysaccharide (LPS) treatment which is well-known as a inflammation-inducing agent. The stems were extracted with hexane, dichloromethane, ethyl acetate, butanol, and water and their nitrite contents in RAW 264.7 cells were measured. The dichloromethane extracts showed the highest inflammatory activity, exhibiting 80% reduction of the nitrite content at 1 mg/mL treatment. Activity-directed fractionation of dichloromethane extracts led to the identification of $\beta$-sitosterol as the anti-inflammatory chemical. 0.1 mg/mL treatment of $\beta$-sitosterol inhibited strongly the production of nitrite by 65%, compared to only LPS treatment. These results suggest that stem of H. rhamnoides L. may be useful for inflammation treatment.

Implementation of Location Based Services Using Satellite DMB System (위성 DMB 시스템을 이용한 위치 기반 서비스 구현)

  • Kwon, Seong-Geun;Lee, Suk-Hwan;Kim, Kang-Wook;Kwon, Ki-Ryong
    • Journal of Korea Multimedia Society
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    • v.15 no.1
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    • pp.32-39
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    • 2012
  • In this paper, the implementation of location based services (LBS) using S-DMB (satellite-digital multimedia broadcasting) system was proposed. In S-DMB System, the frequency of transmitted signal is about 2 GHz which has a characteristics of strong straightness but weak diffraction so that there are many shade areas such as indoors and underground spaces. Therefore the signal transmitted from the satellite should be retransmitted by the earth repeaters called as gap filler. Because each gap filler has its own identification value, the gap filler ID introduces the area in which the gap filler was installed. Generally, the 51st data symbols of S-DMB pilot signal transmitted from the satellite are padded by dummy value and gap filler ID is embedded in this pilot symbol by the gap filler when S-DMB signals are retransmitted by gap fillers. So using gap filler ID of S-DMB system, LBS such as region registration, distance and time to destination, alarm of local area information could be implemented. In the experiment to prove the performance of the proposed LBS system using the gap filler ID of the S-DMB system, the firmware of S-DMB chip composing of RF and baseband parts was lightly modified so that application processor was able to manipulate the gap filler ID and the its related regional information.

Isolation and characterization of new Methanosarcina mazei strains KOR-3, -4, -5, and -6 from an anaerobic digester using pig slurry

  • Battumur, Urantulkhuur;Yoon, Youngman;Bae, Gui Sek;Kim, Chang-Hyun
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.8
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    • pp.1198-1205
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    • 2017
  • Objective: An experiment was conducted to isolate and identify new methanogens in Korea from an anaerobic digester that uses pig slurry. Methods: An anaerobic digestate sample was collected from an anaerobic digester using pig slurry. Pre-reduced media were used for the growth and isolation of methanogens. Growth temperature range, pH range, NaCl concentration range, substrate utilization, and antibiotic tolerance were investigated to determine the physiological characteristics of isolated methanogens. The isolates were also examined microscopically for their morphology and Gram-stained. Polymerase chain reaction of 16S rRNA and mcrA gene-based amplicons was used for identification purpose. Results: Four strains, designated KOR-3, -4, -5, and -6, were isolated and were non-motile, irregular coccoid, and 0.5 to $1.5{\mu}m$ in diameter. Moreover, the cell walls of isolated strains were Gram-negative. KOR-3 and KOR-4 strains used acetate for methane production but did not use $H_2+CO_2$, formate, or methanol as a growth substrate KOR-5 and KOR-6 strains utilized acetate, methanol, and trimethylamine for methanogenesis but did not use $H_2+CO_2$ or formate as a growth substrate. The optimum temperature and pH for growth of four strains were $39^{\circ}C$ and 6.8 to 7.2, respectively. The optimum concentration of NaCl for growth of KOR-3, KOR-5, and KOR-6 were 1.0% (w/v). The optimum NaCl concentration for KOR-4 was 0.5% (w/v). All of the strains tolerated ampicillin, penicillin G, kanamycin, streptomycin, and tetracycline; however, chloramphenicol inhibited cell growth. Phylogenetic analysis of 16S rRNA and mcrA genes demonstrated that strains KOR-3, -4, -5, and -6 are related to Methanosarcina mazei (M. mazei, 99% sequence similarity). Conclusion: On the basis of physiological and phylogenetic characteristics, strains KOR-3, -4, -5, and -6 are proposed to be new strains within the genus Methanosarcina, named M. mazei KOR-3, -4, -5, and -6.

Method of Walking Surface Identification Technique for Automatic Change of Walking Mode of Intelligent Bionic Leg (지능형 의족의 보행모드 자동변경을 위한 보행노면 판별 기법)

  • Yoo, Seong-Bong;Lim, Young-Kwang;Eom, Su-Hong;Lee, Eung-Hyuk
    • Journal of rehabilitation welfare engineering & assistive technology
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    • v.11 no.1
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    • pp.81-89
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    • 2017
  • In this paper, we propose a gait pattern recognition method for intelligent prosthesis that enables walking in various environments of femoral amputees. The proposed gait mode changing method is a single sensor based algorithm which can discriminate gait surface and gait phase using only strain gauges sensor, and it is designed to simplify the algorithm based on multiple sensors of existing intelligent prosthesis and to reduce cost of prosthesis system. For the recognition algorithm, we analyzed characteristics of the ground reaction force generated during gait of normal person and defined gait step segmentation and gait detection condition, A gait analyzer was constructed for the gait experiment in the environment similar to the femoral amputee. The validity of the paper was verified through the defined detection conditions and fabricated instruments. The accuracy of the algorithm based on the single sensor was 95%. Based on the proposed single sensor-based algorithm, it is considered that the intelligent prosthesis system can be made inexpensive, and the user can directly grasp the state of the walking surface and shift the walking mode. It is confirmed that it is possible to change the automatic walking mode to switch the walking mode that is suitable for the walking mode.