• 농업과학연구
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• 제48권2호
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• pp.283-297
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• 2021

Investigation of genetic diversity and molecular characterization in high yielding rice varieties is important for their identification. The experiment was conducted during 2016 - 2017 to analyse the genetic diversity of fifteen high yielding rice varieties in Bangladesh by using random amplification of polymorphic DNA (RAPD) markers. Polymorphism was revealed in 12 RAPD primers out of 30, whereas no other reaction was detected on the remaining 18 primers. The 40 out of 45 bands (88.89%) polymorphics were produced by the primers and ranged from 50 to 100%. The maximum number of polymorphic bands was produced by the primer OPB-18 whereas the lowest number of polymorphic bands belonged to OPC-12. The genetic similarity coefficients were determined with the RAPD data, which ranged from 0.47 to 0.94. The unweighted paired group of arithmetic means (UPGMA) dendrogram presented the studied rice varieties into two major clusters. Moreover, the value of Nei's genetic diversity is 0.26 and the Shanon information index is 0.41. The study produced distinct positions, suggesting that the genotypes were different from each other. The results indicated that these markers could be efficient for comparing the genetic relationships, patterns of variation, and measurement of genetic distance among rice varieties. Considering all of these results, RAPD analysis is found to be an effective tool for estimating the genetic diversity of different rice varieties. The outcomes of this research may contribute to the germplasm data of rice accessions and a future breeding program of rice genotypes.

• 전기전자학회논문지
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• 제25권1호
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• pp.193-200
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• 2021

불법어업 방지와 유실어구 저감을 위해 해양환경에서 상시적이고 연속적인 어구 모니터링 시스템 개발이 필요하며, 본 논문에서는 수산자원 고갈로 점차 어로활동 범위가 넓어지는 현실과 열악한 해상환경을 고려한 25Km 이상의 통신 커버리지를 갖는 장기적 운용 가능한 신뢰성 있는 시스템 모델을 설계하고, 설계결과를 구현하여 14Km 떨어진 해상환경에 부이와 무선노드를 각각 설치하여 상용망과 Private LoRa망을 통한 통신 성공률을 구분 확인하고 수집된 위치정보를 통한 각 관제시스템의 관제기능을 확인함으로써 시스템의 운용 가능성을 검증하였다.

• 정보보호학회논문지
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• 제30권6호
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• pp.1013-1021
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• 2020

악성코드 저자 식별은 알려진 악성코드 저자의 특징을 이용하여 알려지지 않은 악성코드의 저자 특징과 비교를 통해 악성코드를 식별하기 위한 연구 분야이다. 바이너리를 이용한 저자 식별 방법은 실질적으로 배포된 악성코드를 대상으로 수집 및 분석이 용이하다는 장점을 갖으나, 소스코드를 이용한 방법보다 특징 활용 범위가 제한된다. 이러한 한계점으로 인해 다수의 저자를 대상으로 정확도가 저하된다는 단점을 갖는다. 본 연구는 바이너리 저자 식별에 한계점을 보완하기 위하여 '바이너리로부터 의미론적 특징 정의'와 '서바이벌 네트워크 개념을 이용한 중복 특징에 대한 허용 범위 정의' 방법을 제안한다. 제안한 방법은 바이너리 정보로부터 Opcode 기반의 그래프 특징을 정의하며, 서바이벌 네트워크 개념을 이용하여 저자별 고유 특징을 선택할 수 있는 허용범위를 정의하는 것이다. 이를 통해 저자별 특징 정의 및 특징 선택 방법을 하나의 기술로 정의할 수 있으며, 실험을 통해 선행연구보다 5.0%의 정확도 향상과 함께 소스코드 기반 분석과 동일한 수준의 정확도 도출이 가능함을 확인할 수 있었다.

• 한국항행학회논문지
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• 제25권1호
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• pp.90-95
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• 2021

본 논문에서는 chipless RFID (radio frequency identification) 태그용 고감도 소형 공진기의 설계 방법을 제안하였다. 제안된 고감도 소형 공진기는 기존의 전계-결합(ELC; electric field-coupled) 공진기에서 커패시터 모양의 스트립 구조 대신에 인터디지털-커패시터(IDC; interdigital-capacitor) 구조를 사용하였다. IDC 구조의 극판 길이가 기존의 커패시터 모양 구조 보다 더 길어 공진기의 등가 커패시턴스가 더 커지고, 이로 인해 레이다 단면적(RCS; radar cross section)의 공진 피크 주파수를 낮출 수 있다. 정사각형 루프의 길이와 스트립의 폭이 같은 두 공진기를 두께 0.8 mm의 RF-301 기판에 제작하였다. 실험 결과, ELC 공진기는 bistatic RCS의 공진 피크 주파수와 값은 4.305 GHz와 -30.39 dBsm이었다. 제안된 IDC 공진기의 경우 bistatic RCS의 공진 피크 주파수와 값은 3.295 GHz와 -36.91 dBsm이었다. 따라서 측정 공진 피크 주파수를 기준으로 공진기 크기가 23.5% 정도 소형화되었다.

• International Journal of Computer Science & Network Security
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• 제22권4호
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• pp.401-407
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• 2022

Electronic media are an integral part of modern civilization; educational practices are no exception, which should change the content orientations, structures and methodological approaches in accordance with the requirements of the educational market. This makes it relevant to find effective and successful configurations in the process of implementing modern educational practices. The purpose of the research lies in determining the basic principles of electronic media and their place in modern education, identifying the effectiveness of teaching disciplines with application of electronic media, as well as establishing the level of assessment by students of the need to involve different types of electronic media in the educational process and professional practice. The research methodology is complex; the descriptive method and methods of observation, analysis and synthesis have been used in the academic paper. The method of pedagogical experiment has become the principal one; the method of questionnaires and statistical methods have been also used. The hypothesis of the academic paper lies in the fact that the involvement of electronic media in the educational process makes it more effective and requires conceptual changes in educational practices. The result of the research manifests in the identification of new opportunities for the use of electronic media, leading to conceptual shifts in the framework of modern educational policies. In the future, it will be appropriate to consider the theoretical aspects of changing worldview models in education and the use of new media in the educational process, their effectiveness and relevance.

• Journal of Ginseng Research
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• 제46권3호
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• pp.357-366
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• 2022

Background: Withania somnifera (Solanaceae), generally known as Indian ginseng, is a medicinal plant that is used in Ayurvedic practice for promoting health and longevity. This study aims to identify the bioactive metabolites from Indian ginseng and elucidate their structures. Methods: Withanolides were purified by chromatographic techniques, including HPLC coupled with LC/MS. Chemical structures of isolated withanolides were clarified by analyzing the spectroscopic data from 1D and 2D NMR, and HR-ESIMS experiment. Absolute configurations of the withanolides were established by the application of NMR chemical shifts and ECD calculations. Anti-adipogenic activities of isolates were evaluated using 3T3-L1 preadipocytes with Oil Red O staining and quantitative real-time PCR (qPCR). Results: Phytochemical examination of the roots of Indian ginseng afforded to the isolation of six withanolides (1-6), including three novel withanolides, withasilolides GeI (1-3). All the six compounds inhibited adipogenesis and suppressed the enlargement of lipid droplets, compared to those of the control. Additionally, the mRNA expression levels of Fabp4 and Adipsin, the adipocyte markers decreased noticeably following treatment with 25 µM of 1-6. The active compounds (1-6) also promoted lipid metabolism by upregulating the expression of the lipolytic genes HSL and ATGL and downregulating the expression of the lipogenic gene SREBP1. Conclusion: The results of our experimental studies suggest that the withasilolides identified herein have anti-adipogenic potential and can be considered for the development of therapeutic strategies against adipogenesis in obesity. Our study also provides a mechanistic rationale for using Indian ginseng as a potential therapeutic agent against obesity and related metabolic diseases.

• Journal of Animal Science and Technology
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• 제64권2호
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• pp.330-342
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• 2022

We aimed to investigate candidate proteins related to long-term caloric restriction and feed efficiency in bovine longissimus dorsi muscle (LM). A total of 31 Korean native steers were randomly distributed to ad libitum (n = 16) or caloric restriction group (n = 15) to conduct two feeding trials for 13 mon. In the first trial (10-18 mon of age), steers were fed with 100% ad libitum (NE_g = 0.63 Mcal/kg) or caloric restriction (80% of the previous day's feed intake of ad libitum group). In the second trial (18-23 mon of age), the energy value of 100% ad libitum diet was 1.13 Mcal/kg NE_g and those in caloric restriction group diet was 0.72 Mcal/kg NE_g. At the endpoint of this experiment, in each group, 6 animals were selected with high (n = 3) or low feed efficiency (n = 3) to collect muscle tissue samples (6 animals/group). From muscle tissues of 23 mo of age, we excavated 9 and 12 differentially expressed (two-fold or more) proteins in a nutritional group and feed efficiency group using two-dimensional electrophoresis, respectively. Of these proteins, heat shock protein beta-6 was up-regulated in both the caloric restriction and the low feed efficiency group. In bovine embryonic fibroblasts, the mRNA expression of heat shock protein beta-6 increased after adipogenic differentiation, however, decreased after myogenic differentiation. Our data provide that heat shock protein beta-6 may be an adipogenic protein involved in the mechanism of caloric restriction and feed efficiency in the LM of the steer.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.141.2-142
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• 2003

A flexuous rod-shaped virus was isolated from Cucurbita pepo leaves showing green mosaic and puckering symptoms at Anseong, Korea. Based on the biological tests, electron microscopy, and reverse transcription-polymerase chain reaction (RT-PCR), the isolate was identified as Papaya ringspot virus type Watermelon (PRSV-W). In the biological test, host range of PRSV-W was limited in the families Cucurbitaceae and Chenopodiaceae. Most susceptible cucurbit species, such as Cucurmis lanatus, Cucurmis sativus, Cucurbita pepo, and Citrullus lanatus, responded to mechanical inoculation by PRSV-W that induce green mosaic, malformation, puckering, and narrow laminae. The local lesion symptoms were produced on the inoculated leaves of Chenopodium maranticolor and C. quinoa PRSV specific primers which amplifies the part of the coat protein (CP) genes, generated a 648 bp product from 6 isolates of PRSV-W, but no amplification had been detected in other viruses including CMV, CGMMV, KGMMV, ZYMV and WMV. In electron microscopy, PRSV particles were flexuous, approximately 780 nm in length and 12 nm in width. PRSV-W is one of the worldwide viruses which has the great economic importance in cucumber, melon, squash, watermelon, and other cultivated cucurbits with ZYMV and WMV. This is the first report of PRSV-W on cucurbits in Korea.

• 예술인문사회 융합 멀티미디어 논문지
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• 제7권3호
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• pp.361-370
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• 2017

본 논문에서는 공격적 성향의 운전자를 판단할 수 있는 기계학습 방식과 설문지 방식을 융합한 운전자 성향 판단 연구의 일환으로 두 방법으로 결정된 운전자 성향정보의 상관성을 분석하였다. 30명의 운전자를 대상으로 설문지를 이용한 주관적 성향을 정보를 수집하고 기계학습 기반의 성향판단 시스템을 이용하여 객관적 성향을 취득하였다. 이 중에서 기계학습 기반의 성향판단 시스템은 운전자행위 성향 분류 모델을 기반으로 설계되었다. 모델을 도출하기 위하여 운전자의 가속 패달과 브레이크 패달 조작 데이터와 HMM 기법을 이용한 기계학습을 수행하였다. 두 가지 방법으로 추정한 공격적 성향정보를 Pearson 방식으로 상관관계를 분석한 결과 높은 상관관계가 있음을 확인하였다. 뿐만 아니라 객관적 성향은 동일한 운전자에 대하여 고유한 특성이 있음을 확인하였다. 본 논문의 실험결과는 향후 두 방법을 융합하는 연구를 수행하기 위한 참고자료가 될 것이다. 또한 운전자의 공격적 성향이 주의어시스트, 운전자 식별, 도난방지 등 지능형 운전자 보조시스템에도 응용 될 수 있음을 확인하였다.

• Ocean Systems Engineering
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• 제13권2호
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• pp.173-193
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• 2023

Reliable prediction of the motion of FOWT (floating offshore wind turbine) and associated mooring line tension is important in both design and operation/monitoring processes. In the present study, a 5MW OC4 semisubmersible wind turbine is numerically modeled, simulated, and analyzed by the open-source numerical tool, OpenFAST and in-house numerical tool, Charm3D-FAST. Another commercial-level program FASTv8-OrcaFlex is also introduced for comparison for selected cases. The three simulation programs solve the same turbine-floater-mooring coupled dynamics in time domain while there exist minor differences in the details of the program. Both the motions and mooring-line tensions are calculated and compared with the DeepCWind 1/50 scale model-testing results. The system identification between the numerical and physical models is checked through the static-offset test and free-decay test. Then the system motions and mooring tensions are systematically compared among the simulated results and measured values. Reasonably good agreements between the simulation and measurement are demonstrated for (i) white-noise random waves, (ii) typical random waves, and (iii) typical random waves with steady wind. Based on the comparison between numerical results and experimental data, the relative importance and role of the differences in the numerical methodologies of those three programs can be observed and interpreted. These comparative-study results may provide a certain confidence level and some insight of potential variability in motion and tension predictions for future FOWT designs and applications.