• Applied Biological Chemistry
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• 제16권1호
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• pp.31-40
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• 1973

Azotobacter vinelandi의 세포추출물(細胞抽出物)들이 Shethna flavoprotein의 free radical 형(型)으로의 전자전달기구(電子傳達機構)에 관하여 연구(硏究)하였다. 전자전달(電子傳達)에 관여도(關與度)가 높은 단백질(蛋白質)로 황색형광성단백질(黃色榮光性蛋白質)(protein I)과 갈색단백질(褐色蛋白質)(protein II)을 정제(精製)하였고 이들은 $N_2$ 기압하(氣壓下) 또는 aceton-dry ice 동결(凍結)저장하에서도 쉽게 실활(失活)되었고 반응속도(反應速度) 역시 너무 완만하여 생체내반응(生體內反應)이기에는 의문점을 보였다. 한편 세포추출물중(細胞抽出物中)의 FMN은 NADH에 의(依)하여 환원이 쉽게 이루어졌으며, 환원형 $FMNH_2$는 비효소적(非酵素的)으로 Shethna flavoprotein 의 free radical 을 형성(形成)시켰으며 , 효소적반응속도(酵素的反應速度)보다 15배(倍)의 높은 반응속도(反應速度)를 보였다. 비록 FMN이 생체내(生體內)에서 타(他)단백질과 비결합형(非結合型)으로 존재(存在)하지 않는다해도 FMN의 전자전달체(電子電達體)의 가능성(可能性)을 제시(提示)하였다.

• 한국식품위생안전성학회지
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• 제27권1호
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• pp.103-107
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• 2012

본 연구에서는 축산물가공품에서 건조필름법과 $TEMPO^{(R)}$ TVC의 총세균수 측정에 대한 유효성을 평가하였다. 이를 위하여 국내 유통 중인 축산물가공품 257건과 대장균과 황색포도상구균을 인위적으로 접종시킨 축산물가공품 87건에서 대하여 총세균수를 측정하였다. 실험결과는 $log_{10}$ 값으로 전환 후 CCFRA Guideline 29에 의하여 두 검사법간의 일치율을 분석하였고, Pearson의 검정법을 이용하여 상관분석을 실시하였다. 식육가공품 중 햄류, 건조저장육, 분쇄가공육제품 및 식육추출가공품에서 두검사법간 93%이상의 일치율이 확인된 반면 소시지류에서는 89.1%의 일치율을 보였다. 이에 반하여 우유류, 치즈류, 아이스크림류, 조제유류에서는 92% 이상의 일치율을 보였고, 알가열성형제품은 100%의 일치율을 보였다. 또한 소시지류(상관계수 $r$ = 0.77)와 치즈류(상관계수 $r$ = 0.89)를 제외한 축산물가공품 모든 유형에서 건조필름법과 $TEMPO^{(R)}$ TVC 검사법간의 상관계수($r$)가 0.9 이상으로 ($P$ < 0.001) 높은 양의 상관관계를 나타내었다. 결론적으로 본 연구에서 $TEMPO^{(R)}$ TVC는 검출한계가 있는 일부 축산물가공품을 제외하고 총세균수 검사에 있어서 효과적인 것으로 나타났다.

• Journal of Dairy Science and Biotechnology
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• 제33권3호
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• pp.167-170
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• 2015

The dairy industry, as part of the broader agricultural sector, is classified as a basic industry to the Korea economy. Basic industries provide income to a region by producing an output, purchasing production inputs, services and labor. An integrated, multidisciplinary approach for the next generation of dairy products with added health benefits represent the direct economic contribution. The commercialization of "nutritional" functional foods can only be successful if the consumer is confident in the scientific validity of the claims. Modern biotechnologies such as genomics, genetic expression and biomarkers of health performance suggested to whole dairy products, such as fluid milk, butter, cheese, ice cream and frozen dessert products (German, 1999). The following definition makes the point that dairy products can provide a nutritional value beyond the basic nutritional requirements: 1) The dairy industry has the opportunity to improve the health and well-being of its customers and/or to reduce their risk of disease through dairy products with added activities. 2) Functional dairy products are those that can be demonstrated to benefit target functions in the body in a way that improves the state of health and /or reduces the risk of disease. They are food products that are consumed as part of a normal diet rather than pills or supplements. 3) Dairy products based on functionality will need to link the scientific basis of such functionality to the communication of its benefit to the general public. 4) Both the efficacy and the safety of the food components with health benefits will require evidence based on the measurement of scientific biomarkers relevant to their biological responses and health end points. 5) Sound evidence from human studies based on intermediate health end points using accepted biomarkers will provide the basis for promotional messages divided into two categories-enhanced function and reduced risk of disease. 6) Success in solving key scientific and technological challenges will only be achieved by interdisciplinary research programs to exploit the scientific concepts in functional dairy science.

• 한국가금학회지
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• 제33권1호
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• pp.65-80
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• 2006

재는 가축분을 중성화 시키며 토양에 산포하면 악취를 저감시키는 역할을 하며 하천에서 P의 양을 감소시킬 수 있다. 그러므로 본 연구에서는 재의 특성과 이들의 첨가가 가져올 환경적인 이용에 관해서 언급하였다. 재는 아직도 물리적 화학적으로 일정한 정의가 없다 (석탄재-CFA, 목재재-WA, 왕겨재-RHA). 이들 재를 형성하고 있는 조건과 조성에 따라 산도는 6.0이하 그리고 12.5이상까지 이른다. CFA를 분중에 첨가하면 분중에 녹아있는 P의 양이 감소되어 토양에 산포하면 수질을 향상시킨다. WA는 WA 자체에 많은 탄소 원자를 갖고 있기 때문에 분에서 발생하는 냄새를 제거할 수 있다. RHA는 재중에 $20{\sim}60%$ 가량이 실리카이다. 현재까지 CFA, WA 그리고 RHA를 토양에 산포하여 미치는 영향에 대한 연구는 많이 되어 있으므로 앞으로 가축분에 첨가하면 분중의 P과 악취를 낮출 수 있는 연구가 필요하다.

• 천문학회보
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• 제46권1호
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• pp.57.2-57.2
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• 2021

Variability in Young Stellar Objects (YSOs) can be caused by time-dependent accretion rates, geometric changes in the circumstellar disks, the stochastic hydromagnetic interactions between stellar surfaces and inner disk edges, reconnections within the stellar magnetosphere, and hot/cold spots on stellar surfaces. We uncover ~1400 variables from a sample of ~5300 YSOs in nearby low-mass star-forming regions using mid-IR light curves obtained from the 5.5-years NEOWISE All Sky Survey. The mid-IR variability traces a wide range of dynamical, physical, and geometrical phenomenon. We classify six types of YSO variability based on their light curves: secular variability (Linear, Curved, Periodic) and stochastic variability (Burst, Drop, Irregular). YSOs in earlier evolutionary stages have higher fractions of variables at all types and higher amplitudes for the variability. Along with brightness variability, we also find a diverse range of secular color variations, which can be attributed to a competitive interplay between the variable accretion luminosity of the central source and the variable extinction by material associated with the accretion process. We compare the variability of known FUors/EXors and VeLLOs/LLSs, which represent two extreme ends (burst versus quiescent) of the episodic accretion process; FUors/EXors have a higher fraction of variables (65%) than VeLLOs/LLSs (41%). Short-term (few day) and long-term (decades) variability, as well as possible AGB contamination in the YSO catalogues, are also discussed.molecules become more complex by surface chemistry induced directly by high energy photons or by the thermal energy diffused over heated grain surface. Therefore, the ice composition is an

• 대한한방종양학회지
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• 제6권1호
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• pp.67-79
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• 2000

Objective : Hedyotis diffusa has been used as an anticancer agent for several decades in oriental medicine. We test whether the methanol extract of the herb affects transcriptional activation factors including $NF-{\kappa}B$ and AP-1. Methods : 1. HL-60 cells were treated with various concentrations(from 200 to $50{\mu}g/ml$) of methanol extract and $H_2O$ extract($200{\mu}g/ml$)of hedyotis diffusa, After 48h later, the cells were tested for viability by MTT assay. 2. The HL-60 cells were treated with $200{\mu}g/ml$ of methanol extract for the indicated periods. First. Nuclear extracts were isolated and incubated with oligonucleotide probe of $NF-{\kappa}B$ and AP-1. Second. Nuclear extracts were isolated and reacted with p50, p65. c-rel pan-Jun, c-Jun, JunB. JunD antibody on ice for 30min. Finally The cell lysates were prepared and analyzed by western blotting using anti-Fas, anti-FasL and anti-p53 antibody. Results : 1. The methanol extract decreases the viability of human lymphoid origin leukemia HL-60 cells in a dose-dependent manner. 2. $NF-{\kappa}B$ is rapidly activated by the addition of the methanol extract, reaches a peak at 30min and gradually returns to resting level. We confirm that $NF-{\kappa}B$ is a heterodimer mainly composed of p65 subunit with c-Rel. 3. Transcriptional activation of AP-1 is detected at 30min and reaches a maximum at 1hr after stimulation of the cells with the methanol extract. AP-1 is mainly composed with Jur-D and partially Jug-B proteins. 4. the methanol extract of Hedyotis diffusa induces the expression of Fas, Fas ligand and p53 proteins of HL-60 cells in a time dependent fashion. Conclusions : These results suggest that the methanol extract of Hedyotis diffusa exerts anticancer effects to induce the death of human leukomic HL-60 cells via activation of trascriptional factors such as $NF-{\kappa}B$ and AP-1, increase in expression of Fas mediated signalling proteins, and induction of tumor suppressor gene. p53.

• 대한영양사협회학술지
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• 제22권2호
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• pp.88-105
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• 2016

This study investigated anthropometric and biochemical characteristics, food and nutrient intakes, and changes in body mass index (BMI) of Korean middle age men (40~49 yrs). Data on BMI, anthropometric (height, weight, waist circumference, body mass index, blood pressure), and biochemical (total cholesterol, HDL-cholesterol, LDL-cholesterol, triglyceride, hemoglobin, GOT, GPT) characteristics, food and nutrients intakes, nutrient density, and index of nutrient quality were obtained from the Fifth Korea National Health and Nutrition Examination Survey (KNHANES, 2001, 2005, 2011). Subjects were divided into three groups by year. Weight, BMI, and hemoglobin increased significantly by year. All anthropometric factors except height showed a positive relation with BMI by year. Consumption of barley, chinese noodle, confections, soybean milk, egg, ice cream, pizza, fried foods, banana, coffee, and raw rice wine increased significantly by year. Intake ratios of nutrients except water and fiber were over 100% of KDRI generally. INQ according to intakes of protein, calcium, phosphorus, iron, vitamin A, thiamine, riboflavin, and niacin per 1,000 kcal increased significantly by year. Distribution of BMI status of subjects showed a significant difference by exercise. The findings of the present study show that BMI of subjects increased by year, and intakes of all nutrients except calcium and riboflavin showed aspects of overnutrition by year. Intakes of fruits decreased while intakes of raw rice wine and coffee increased by year. Therefore, practical and systematic nutrition programs are required to maintain optimum BMI and appropriate intakes of nutrients to maintain a desirable lifestyle and improve dietary life.

• The Journal of Korean Physical Therapy
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• 제17권4호
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• pp.457-467
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• 2005

Physiotherapyists frequently use manipulative therapy technique to treat dysfunctionand pain resulting from ankle sprain. Despite the high prevalence of lateral ankle ligament injuries, few studies are available indicating any physical associations with the development of lateral ankle ligament injuries, or information of treatment for lateral ankle ligament injuries. To investigate the effect of passive joint mobilization, the anteroposterior glide on the talus, on increasing weight-bearing dorsiflexion, single support time and VAS. Sixty lateral ankle ligament injuries (grade I and grade II) aged between 17 and 27 years (mean age 21) were recruited. Subjects were randomly assigned to 1 of 2 treatment groups. The control group received a protocol of rest, ice, compression, and elevation (RICE) and massage. The experimental group received the anteroposterior mobilization, using a force that avoided incurring any increase in pain, in addition to the RICE protocol. Subjects in both groups were treated every second day for a maximum of 2 weeks or until the discharge criteria were met, and all subjects were given a home program of continued RICE application. Outcomes were measured after each treatment. The results showed that the experimental group than the control group. Weight-bearing dorsiflexion (F=7.640, P<0.05), single support time (F=85.532, P<0.05) and VAS (F=10.610, P<0.050). Between-groups differences were observed as; experimental group is increased weight-bearing dorsiflexion, single support time and reduced VAS.

• 한국전기전자재료학회논문지
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• 제27권9호
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• pp.600-605
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• 2014

The interest in development on luminaires which are available up to $-52^{\circ}C$ is surging as demands in vessels navigating a north pole route increase. A conventional incandescent lamp used in vessels is operated stably at $-52^{\circ}C$, but many countries including Korea have eliminated the use of incandescent lamps gradually because of its low luminous efficacy. In this paper, therefore, to develop the LED luminaires with high-efficiency, long lifetime that enables to substitute for incandescent lamp, it has studied about cryogenic characteristics of LED packages, bulbs, driving circuit and power supply. This experiments were carried out according to standards IEC 60945-8.4.1. Temperature range is from $-60^{\circ}C$ to $25^{\circ}C$, and the light output depending on ambient temperature. It showed that, based on $25^{\circ}C$, light output of a CFL decreased by 80% of CFL at $-20^{\circ}C$ while each increased 12% of LED bulbs and 16~19% of LED packages at $-60^{\circ}C$.

• 지구물리
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• 제9권4호
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• pp.397-408
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• 2006

남극 반도와 그 주변에서의 온난화는 시간적, 공간적 변동이 크기 때문에 이 지역에서의 국지 기후를 이해하는 것이 필요하다. 이 연구의 목적은 남극 세종 기지 주변에서의 지표 복사, 기온 그리고 풍향의 특징을 밝혀내고, 그 변수들간의 상관 관계를 조사하는 것이다. 연구지는 연간 15-20 Wm-2의 복사 에너지를 흡수하지만, 여름 (12월-1월)에는 평균적으로 85 Wm-2의 복사 에너지를 흡수하였다. 이 흡수량은 남극의 다른 지역에 비해 상당히 큰 값이다. 월 평균 기온은 -7.7-2.8oC 이었으며, 매년 다른 변동을 보였다. 북서, 서 및 동풍이 주풍이었으며, 풍향의 변동이 기온 변동을 설명할 수 있었다. 찬 동풍과 따뜻한 북서 및 북풍 빈도의 변동이 특히 여름철 지표 복사 수지에도 크게 영향을 주었으며, 이 시기의 대기와 지면 간의 에너지 교환에 풍향이 중요한 역할을 하는 것으로 보인다.