• Molecules and Cells
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• 제43권12호
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• pp.1011-1022
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• 2020

Cell type specification is a delicate biological event in which every step is under tight regulation. From a molecular point of view, cell fate commitment begins with chromatin alteration, which kickstarts lineage-determining factors to initiate a series of genes required for cell specification. Several important neuronal differentiation factors have been identified from ectopic over-expression studies. However, there is scarce information on which DNA regions are modified during induced pluripotent stem cell (iPSC) to neuronal progenitor cell (NPC) differentiation, the cis regulatory factors that attach to these accessible regions, or the genes that are initially expressed. In this study, we identified the DNA accessible regions of iPSCs and NPCs via the Assay for Transposase-Accessible Chromatin sequencing (ATAC-seq). We identified which chromatin regions were modified after neuronal differentiation and found that the enhancer regions had more active histone modification changes than the promoters. Through motif enrichment analysis, we found that NEUROD1 controls iPSC differentiation to NPC by binding to the accessible regions of enhancers in cooperation with other factors such as the Hox proteins. Finally, by using Hi-C data, we categorized the genes that directly interacted with the enhancers under the control of NEUROD1 during iPSC to NPC differentiation.

• 대한화학회지
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• 제29권5호
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• pp.478-481
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• 1985

염화 알칸술포닐과 파라 치환된 아닐린간의 친핵성 치환반응을 메탄올-아세토니트릴 혼합용매하에서 연구하였다. 기질의 반응성, 친핵체의 치환기효과 및 용매효과로 부터 반응 메카니즘이 $S_N2$임을 알았다. i-PSC의 경우 메탄올 조성이 커짐에 따라 전이상태가 더 loose해 짐을 알았다.

• 한국방재학회 논문집
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• 제9권1호
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• pp.65-71
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• 2009

본 연구에서는 기존의 25 m 지간을 갖는 PSC I형 단순 철도교에 대해 고속 및 일반 열차하중으로 인한 동적거동을 분석하여 철도교량의 동적사용성을 평가하였다. 고유진동수는 8Hz 대역으로 평가되어 철도교량의 적정 고유진동수 범위 내에 들어있으며, 공진발생 가능성은 없는 것으로 나타났다. 가속도 응답은 무궁화호 열차 주행 시 제한 값 0.35 g를 초과하는 0.43 g가계측되었다. 또한 단부꺽임각은 고속철도의 설계기준을 만족하지 못하였으며 충격계수와 처짐은 모두 설계기준을 만족하였다. 결과적으로 25 m 지간을 갖는 PSC I형 단순 철도교의 경우 다양한 열차하중에 대하여 동적사용성을 부분적으로 확보하고 있는 것으로 나타났으나 진동가속도 응답을 감소시키기 위한 대책이 필요할 것으로 판단된다.

• BMB Reports
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• 제52권12호
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• pp.689-694
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• 2019

Ethical and safety issues have rendered mesenchymal stem cells (MSCs) popular candidates in regenerative medicine, but their therapeutic capacity is lower than that of induced pluripotent stem cells (iPSCs). This study compared original, dental tissue-derived MSCs with re-differentiated MSCs from iPSCs (iPS-MSCs). CD marker expression in iPS-MSCs was similar to original MSCs. iPS-MSCs expressed higher in pluripotent genes, but lower levels in mesodermal genes than MSCs. In addition, iPS-MSCs did not form teratomas. All iPSCs carried mtDNA mutations; some shared with original MSCs and others not previously detected therein. Shared mutations were synonymous, while novel mutations were non-synonymous or located on RNA-encoding genes. iPS-MSCs also harbored mtDNA mutations transmitted from iPSCs. Selected iPS-MSCs displayed lower mitochondrial respiration than original MSCs. In conclusion, screening for mtDNA mutations in iPSC lines for iPS-MSCs can identify mutation-free cell lines for therapeutic applications.

• 한국구조물진단유지관리공학회 논문집
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• 제25권6호
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• pp.50-59
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• 2021

현재 공용중인 교량에 대한 재하시험으로 계측한 응답과 초기해석모델의 해석 응답을 비교하여 응답보정계수를 계산하고, 이 모델을 사용하여 내하율과 내하력을 평가하는 방법을 적용하고 있다. 그러나 이러한 응답보정계수는 계측 위치와 하중 조건에 따라 변동하는 값을 준다. 특히 초기해석모델이 교량의 거동에 합당하지 않은 경우, 그 변동 폭이 크며 보정된 모델에 의한 해석 응답이 계측된 응답과 동떨어진 결과를 줄 수 있다. 본 연구에서는 저속으로 주행하는 차량의 다양한 하중 조건에서 동적 성분을 제거한 정적 응답을 얻기 위해 의사정적재하시험법을 적용하였다. 두 개의 유사한 PSC-I 거더교에서 정적 응답을 계측하고, 두 교량 각각에 대한 변위와 변형률에 대한 응답보정계수를 계산하였다. 초기해석모델이 제대로 설정되어 있지 않다면, 평균적으로 구한 응답보정계수로 보정한 모델의 해석 응답이 계측 응답과 오차범위 내에 들어오는지 않음을 확인하였다.

• 대한토목학회논문집
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• 제34권6호
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• pp.1731-1741
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• 2014

교통수요의 증가에 따른 교통혼잡을 피하기 위하여 고가도로와 고속도로, 경전철 등이 활발히 건설되고 있으며, 이에 따라 접속부 등과 같은 곡률반경이 작은 곡선교 구간의 발생이 증가하고 있다. 초기에는 직선형의 주형을 연결하여 곡선을 이루는 방법을 사용하였으나, 공사비의 증가와 미관성 측면에서 용이하지 않아 강재를 사용한 곡선주형이 개발되어 적용되고 있다. 강재의 특성상 기하학적인 형상을 구현하는데 상대적으로 손쉽기 때문에 대부분의 곡선교에서는 강재를 이용한 강박스 거더교를 주로 사용하고 있다. 그러나 강박스 거더교는 초기 공사비가 고가이고, 도장과 같은 지속적인 유지관리가 필요한 문제점을 가지고 있다. 최근에는 I형 강재 플레이트 거더를 사용하는 방법이 활발히 연구되고 있으나 곡선 I형상의 특성상 개단면이고 얇은 플레이트를 사용하기 때문에 비틀림 강성이 매우 작아 안전성에 문제가 있는 것으로 지적되고 있다. 이러한 곡선강교의 대안으로 경제성과 안전성이 확보되는 프리캐스트 PSC 곡선거더가 제시되고 있다. 이에 본 연구에서는 프리캐스트 PSC 곡선거더를 효율적으로 제작할 수 있는 스마트 몰드 시스템을 개발하고 이를 적용한 2주형 40m 실물교량의 정적 휨 파괴 실험을 통하여 안전성을 확인하고자 한다. 제작단계에서 단일 거더는 곡률반경에 의해 거더의 전도에 대한 안정성이 문제가 있으나 가로보로 연결된 멀티거더는 하중분배가 적절히 이루어지며 안정성이 확보되는 것으로 나타났다. 정적구조 실험결과에서는 균열하중(1,400 kN)이 설계하중(450 kN)의 약 3배 이상 높은 것으로 나타났으며, 허용처짐량에 해당하는 하중은 설계하중의 4배인 1,800 kN에서 발생하여 사용성과 안전성이 모두 확보되는 것으로 나타났다.

• International Journal of Concrete Structures and Materials
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• 제4권2호
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• pp.77-87
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• 2010

Prestressed concrete (PSC) I-type girders have been used for span length around up to 40 m in domestic region. PSC girders are very cost effective girder type and extending their lengths more than 50 m will bring large benefit in cost. A new design method was proposed by combining two notable design concept in order to extend the applicable span length in this study. First of all, several numbers of openings was introduced in the girder web, and half of the anchorage devices were moved into the openings. In this way, large compressive stress developed at end zone was reduced, and the portion of design load coming from self-weight was reduced as well. Secondly, prestressing force was introduced in the girder not once at the initial stage, but through multiple loading stages. A full scale girder with the length of 50 m with the girder depth of 2 m was fabricated, and a flexural test was conducted in order to verify the performance of newly developed girder. Test results showed that the new holed web design concept can provide a way to design girders longer than 50 meters with the girder height of 2 m.

• International Journal of Railway
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• 제3권3호
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• pp.83-89
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• 2010

This paper attempts to extract the fundamental dynamic properties, i.e. natural frequencies, damping ratios of the 48 m-long, $20^{\circ}$ skewed real bridge with PSC girders wrapped by a steel plate. The forced vibration test is achieved by mounting 12 Hz-capacity of artificial oscillator on the top of bridge deck. The acceleration histories at the 9 different locations of deck surface are recorded using accelerometors. From this full-scaled vibration test, the two possible resonance frequencies are detected at 2.38 Hz and 9.86 Hz of the skewed bridge deck by sweeping a beating frequency up to 12 Hz. The absolute acceleration/energy exhibits much higher in case of higher-order twist mode, 9.86 Hz due to the skewness of bridge deck which leads asymmetric situation of vibration. This implies the test bridge is under swinging vertically in fundamental flexure mode while the bridge is also flickered up and down laterally at 9.86 Hz. This is probably by asymmetric geometry of skewed deck. A detailed 3D beam-shell bridge models using finite elements are performed under a series of train loads for modal dynamic analyses. Thereby, the effect of skewness is examined to clarify the lateral flickering caused by asymmetrical geometry of bridge deck.

• 한국동물생명공학회지
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• 제38권4호
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• pp.199-208
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• 2023

Background: Canine induced pluripotent stem cells (iPSCs) are an attractive source for veterinary regenerative medicine, disease modeling, and drug development. Here we used vitamin C (Vc) to improve the reprogramming efficiency of canine iPSCs, and its functions in the reprogramming process were elucidated. Methods: Retroviral transduction of Oct4, Sox2, Klf4, c-Myc (OSKM), and GFP was employed to induce reprogramming in canine fetal fibroblasts. Following transduction, the culture medium was subsequently replaced with ESC medium containing Vc to determine the effect on reprogramming activity. Results: The number of AP-positive iPSC colonies dramatically increased in culture conditions supplemented with Vc. Vc enhanced the efficacy of retrovirus transduction, which appears to be correlated with enhanced cell proliferation capacity. To confirm the characteristics of the Vc-treated iPSCs, the cells were cultured to passage 5, and pluripotency markers including Oct4, Sox2, Nanog, and Tra-1-60 were observed by immunocytochemistry. The expression of endogenous pluripotent genes (Oct4, Nanog, Rex1, and telomerase) were also verified by PCR. The complete silencing of exogenously transduced human OSKM factors was observed exclusively in canine iPSCs treated with Vc. Canine iPSCs treated with Vc are capable of forming embryoid bodies in vitro and have spontaneously differentiated into three germ layers. Conclusions: Our findings emphasize a straightforward method for enhancing the efficiency of canine iPSC generation and provide insight into the Vc effect on the reprogramming process.

• International Journal of Stem Cells
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• 제16권1호
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• pp.36-43
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• 2023

Background and Objectives: Lymphoblastoid cell lines (LCLs) deposited from disease-affected individuals could be a valuable donor cell source for generating disease-specific induced pluripotent stem cells (iPSCs). However, generation of iPSCs from the LCLs is still challenging, as yet no effective gene delivery strategy has been developed. Methods and Results: Here, we reveal an effective gene delivery method specifically for LCLs. We found that LCLs appear to be refractory toward retroviral and lentiviral transduction. Consequently, lentiviral and retroviral transduction of OCT4, SOX2, KFL4 and c-MYC into LCLs does not elicit iPSC colony formation. Interestingly, however we found that transfection of oriP/EBNA-1-based episomal vectors by electroporation is an efficient gene delivery system into LCLs, enabling iPSC generation from LCLs. These iPSCs expressed pluripotency makers (OCT4, NANOG, SSEA4, SALL4) and could form embryoid bodies. Conclusions: Our data show that electroporation is an effective gene delivery method with which LCLs can be efficiently reprogrammed into iPSCs.