• Journal of Life Science
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• v.29 no.8
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• pp.895-903
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• 2019

The aim of the present study was to improve the cell viability of human dental papilla derived single-induced pluripotent stem cells (iPSCs) using a Rho-associated protein kinase (ROCK) inhibitor, Y-27632. The iPSCs were produced using an episomal plasmid-based reprogramming method. After cell separation using trypsin, the iPSCs were treated with 0, 0.5, 1, 2.5, 5, 7.5, or $10{\mu}M$ Y-27632 for 5 d. Cell viability increased significantly following the $5{\mu}M$ Y-27632 treatment (p<0.05). When the iPSCs were exposed to medium containing $10{\mu}M$ Y-27632 for 0, 1, 2, 3, 4, and 5 d, the cell viability rate increased significantly in accordance with the cell viability rate (p<0.05). To evaluate the effect of the Y-27632 treatment on stemness characteristics, the expression of stem cell-specific transcripts and telomerase activity were investigated in the iPSCs treated with $10{\mu}M$ Y-27632 for 5 d. The expression levels of stem cell-specific transcripts, such as OCT-4, NONOG, and SOX-2, and telomerase activity were not significantly different in the iPSCs treated with $10{\mu}M$ Y-27632 as compared with those of untreated control iPSCs (p>0.05). Taken together, the results demonstrated that cell viability can be improved by treatment with the ROCK inhibitor Y-27632, without losing iPSC stemness characteristics.

• Journal of the Korean Society for Railway
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• v.15 no.2
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• pp.154-161
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• 2012

The damping ratio of railway bridge has become one of the most important issues in dynamic design and dynamic stability of railway bridge. In the present study, laboratory and field test were performed for railway bridges such as a twin I-shaped steel composite girder, PSC box, steel box, PSC, IPC, PRECOM, preflex. The damping ratio of railway bridge according to structure types was estimated by logarithmic decrement method. Therefore, magnitude, frequency and amplitude of load did not affect damping ratio of railway bridge. Also, damping ratio limit of steel composite and PSC bridges was evaluated in 1.0%.

• Proceedings of the Korea Concrete Institute Conference
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• 1998.10b
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• pp.680-685
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• 1998

Prestressed concrete I-girders were used in the bridge applications in the early 1950s. During the last four decades, the most widely used girder length of bridges have been below 30meters. The main objective of this study is to develope the alternative section for long span bridge which exceed 40 meters. The developed Bulb-Tee girder has a wide bottom flange to enhance the compressive strength and to allow placement of a large number of strands in the bottom flange. New bulb-tee shaped PSC girder sections are proposed in this paper. Splicing the technique for long span bridge girder to reduce the self weight is also proposed.

• Food Science of Animal Resources
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• v.36 no.5
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• pp.665-670
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• 2016

The objectives of this study were conducted to characterize pepsin-soluble collagen (PSC) extracted from bones (PSC-B), skins (PSC-S), and tendons (PSC-T) of duck feet and to determine their thermal and structural properties, for better practical application of each part of duck feet as a novel source for collagen. PSC was extracted from each part of duck feet by using 0.5 M acetic acid containing 5% (w/w) pepsin. Electrophoretic patterns showed that the ratio between α₁ and α₂ chains, which are subunit polypeptides forming collagen triple helix, was approximately 1:1 in all PSCs of duck feet. PSC-B had slightly higher molecular weights for α₁ and α₂ chains than PSC-S and PSC-T. From the results of differential scanning calorimetry (DSC), higher onset (beginning point of melting) and peak temperatures (maximum point of curve) were found at PSC-B compared to PSC-S and PSC-T (p<0.05). Fourier transform infrared spectroscopy (FT-IR) presented that PSC-S and PSC-T had similar intermolecular structures and chemical bonds, whereas PSC-B exhibited slight difference in amide A region. Irregular dense sheet-like films linked by random-coiled filaments were observed similarly. Our findings indicate that PSCs of duck feet might be characterized similarly as a mixture of collagen type I and II and suggest that duck feet could be used for collagen extraction without deboning and/or separation processes.

• 해운
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• no.11 s.10
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• pp.2-13
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• 2004

• Korean Circulation Journal
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• v.48 no.11
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• pp.974-988
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• 2018

Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), which are collectively called pluripotent stem cells (PSCs), have emerged as a promising source for regenerative medicine. Particularly, human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have shown robust potential for regenerating injured heart. Over the past two decades, protocols to differentiate hPSCs into CMs at high efficiency have been developed, opening the door for clinical application. Studies further demonstrated therapeutic effects of hPSC-CMs in small and large animal models and the underlying mechanisms of cardiac repair. However, gaps remain in explanations of the therapeutic effects of engrafted hPSC-CMs. In addition, bioengineering technologies improved survival and therapeutic effects of hPSC-CMs in vivo. While most of the original concerns associated with the use of hPSCs have been addressed, several issues remain to be resolved such as immaturity of transplanted cells, lack of electrical integration leading to arrhythmogenic risk, and tumorigenicity. Cell therapy with hPSC-CMs has shown great potential for biological therapy of injured heart; however, more studies are needed to ensure the therapeutic effects, underlying mechanisms, and safety, before this technology can be applied clinically.

• Journal of Korean Society of Disaster and Security
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• v.16 no.3
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• pp.17-26
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• 2023

Prestressed Concrete (PSC) girders are divided into pre- and post-tension types as prestressing method, and I- and U-type as cross-sectional shape. There are both advantages and disadvantages depending on each prestressing method and cross-sectional shape, and each method is applied to bridge construction sites. In this study, a new girder design was attempted to develop that overcomes its shortcomings by using the pretension method and U-type cross sectional shape. Its structural performance was verified in this study. Pretension type girders are mainly manufactured in factories because they require a reaction arm and related facilities, and have the disadvantage of being limited in weight and span length for road transportation. In addition, in the case of the U-type cross-section, structural stability is very reliable during construction against overturning, but its own weight is relatively large comparing to I-type, and the post-tension method is mainly applied after on-site production. In this study, a PSC girder manufacturing method using the field pretension was proposed and a span length of 40 m real-scale test specimen was manufactured and verified its structural performance.

• Journal of the Korean Society for Railway
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• v.19 no.2
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• pp.187-194
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• 2016

It is necessary to determine reliability indexes of existing railway bridges prior to setting up a proper target reliability index that can be used to introduce a reliability based limit state design method to design practice. Reliability is evaluated for a six PSC-I girder railway bridge, which is one of many representative types of double-track railway bridges. The reliability assessment is carried out for an edge girder subjected to bending moment. In the assessment, the flexural resistance and the fixed-load effect were obtained using existing statistical values from previous research on the introduction of limit state design to road bridge design. On the other hand, the live-load effect was determined using statistical values obtained from field measurement for the Joong-ang corridor, on which heavy freight trains are frequently passing. The reliability assessment is performed by AFOSM(Advanced First Order Second Moment method) for the limit state equation, and a sensitivity analysis for the reliability is performed for each factor of the load and resistance effects.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.6
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• pp.742-752
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• 2017

The bridge type of steel concrete composite rahmen or pre-flex has been applied where a lower depth girder is required due to vertical clearance restrictions caused by the crossing of rivers and roads. On the other hand, because these types are not only complicated to manufacture and construct, but also expensive, void slab bridges may be an alternative. In this study, prefabricated PSC-I shape girder was used to make a void slab and all procedures for bridge development, such as analysis, design, fabrication, and real-scale test, were included in the scope of research. The results of this study will provide sufficient background data to be applied to the field and the structural safety has been verified through experiments.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.26 no.3A
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• pp.421-428
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• 2006

This study was performed by using experiment to minimize behavior difference of Monolithic and segmental Girder and to prove the design concept of the PSI (Precast PSC-Segmental I Grider). A full scale girder test was performed in four different cases, the monolithic girder, the segmental girder type-1, the segmental girder type-2 and the segmental girder type-3. The monolithic girder that was produced in one body 25 m span and the segmental girder that was jointed 5-sliced 5 m segment. The girder was built by as one body prestressing the tendons after manufacturing the segmental girder, and second prestressing after the casting of the slab concrete. The test result shows that the measured values were almost same or slightly bigger than the theoretical values which means that the PSI girder bridges concept came out to be reliable.