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http://dx.doi.org/10.5352/JLS.2019.29.8.895

Improvement of Cell Viability Using a Rho-associated Protein Kinase (ROCK) Inhibitor in Human Dental Papilla derived Single-induced Pluripotent Stem Cells  

Shim, Yoo-Jin (Department of Biology Education, Gyeongsang National University)
Kang, Young-Hoon (Department of Oral and Maxillofacial Surgery, Gyeongsang National University)
Kim, Hyeon-Ji (Department of Biology Education, Gyeongsang National University)
Kim, Mi-Jeong (Department of Biology Education, Gyeongsang National University)
Lee, Hyeon-Jeong (OBS/Theriogenology and Biotechnology, Gyeongsang National University)
Son, Young-Bum (OBS/Theriogenology and Biotechnology, Gyeongsang National University)
Lee, Sung-Ho (Division of Life Science, Gyeongsang National University)
Jeon, Byeong-Gyun (Department of Biology Education, Gyeongsang National University)
Publication Information
Journal of Life Science / v.29, no.8, 2019 , pp. 895-903 More about this Journal
Abstract
The aim of the present study was to improve the cell viability of human dental papilla derived single-induced pluripotent stem cells (iPSCs) using a Rho-associated protein kinase (ROCK) inhibitor, Y-27632. The iPSCs were produced using an episomal plasmid-based reprogramming method. After cell separation using trypsin, the iPSCs were treated with 0, 0.5, 1, 2.5, 5, 7.5, or $10{\mu}M$ Y-27632 for 5 d. Cell viability increased significantly following the $5{\mu}M$ Y-27632 treatment (p<0.05). When the iPSCs were exposed to medium containing $10{\mu}M$ Y-27632 for 0, 1, 2, 3, 4, and 5 d, the cell viability rate increased significantly in accordance with the cell viability rate (p<0.05). To evaluate the effect of the Y-27632 treatment on stemness characteristics, the expression of stem cell-specific transcripts and telomerase activity were investigated in the iPSCs treated with $10{\mu}M$ Y-27632 for 5 d. The expression levels of stem cell-specific transcripts, such as OCT-4, NONOG, and SOX-2, and telomerase activity were not significantly different in the iPSCs treated with $10{\mu}M$ Y-27632 as compared with those of untreated control iPSCs (p>0.05). Taken together, the results demonstrated that cell viability can be improved by treatment with the ROCK inhibitor Y-27632, without losing iPSC stemness characteristics.
Keywords
Anoikis; human; iPSCs; stem cells; Y-27632;
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