• Journal of Aquaculture
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• v.9 no.3
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• pp.293-300
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• 1996

Validities of several gene transfer methods including microinjection, electroporation and lipo-fection with luciferase gene (pRSVL), and effectiveness of mud loach expression vector which contains ARS from mud loach on production of transgenic mud loach were evaluated. Microiniection revealed the $0\~8\%$ of transgene incidence in 2-week-old fish with significant mosaicism. Electroporation and lipofection of mud loach sperm also successfully introduced the transgene into sperm cells, and transferred the foreign DNA into zygote. Gene transfer by electroporation and lipofection showed a range of $0\~28\%$ and $0\~48.1\%$ of transgene incidence, respectively in newly hatched larvae, altough most DNA introduced were gradually degraded with the development of fish. Microinjections of mud loach expression vector caused a significantly reduced survival rate of mud loach embryos with severe teratogenic effects, and ARS/Luc transgene could not be detected in normally developed fish after microinjection.

• Korean Journal of Veterinary Research
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• v.29 no.3
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• pp.407-416
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• 1989

The prospect of live vaccines consisting of genetically modified vaccinia virus expressing foreign genes is exciting, but important issues concerning safety and efficacy need to resolved. Vaccinia virus (VV) is an efficient expression vector with broad host range infectivity and large DNA capacity. This vector has been particularly useful for identifying target antigens for humoral and cell-mediated immunity. The WHO smallpox eradication program, involving the extensive use of VV vaccines, resulted in the late 1970s in the elimination of one of the world's most feared diseases. This achievement is a triumph for preventive medicine and for international collaboration in public health. In 1980, WHO recommended that the routine use of smallpox vaccine should be stopped. Against this background, the prospect of li ve vaccines consisting of genetically modified VV expressing foreign antigens arising from the work of Moss, and Paoletti and their colleagues in 1982 has been greeted with enthusiasm. These investigators have shown that genes coding for immunogenic proteins can be inserted into VV DNA without impairing the ability of the virus to grow in cell culture. Moreover experimental animals infected with VV recombinants containing genes coding for a variety of immunizing proteins have been shown to be protected against challenge infection with the corresponding infectious agent. In this communication, I describe current progress in the construction of a novel plasmid vector that facilitate the insertion and expression of foreign genes in VV as well as the selection of recombinants.

• Journal of Microbiology and Biotechnology
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• v.1 no.4
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• pp.240-245
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• 1991

We have constructed a promoter-probe vector pKU20 using pKT230, a derivative of broad-host-range plsmid RSF1010, as a base. The pKU20 contains structural gene for aminoglycoside phos-photransferase (aph), without promoter, and a multiple cloning site upstream the aph. Using this vector, a 412base pairs (bp) PstI fragment showing strong promoter activity both in Escherichia coli LE392 and Pseudomonas putida KCTC1644 has been cloned from Pseudomonas fluorescens chromosomal DNA on the basis of streptomycin resistance. The nucleotide sequence of the 412 bp fragment has been determined and the putative - 35 and -10 region was observed. Insecticidal protein gene of Bacillus thuringiensis subsp. kurstaki HD-73 inserted on downstream of the promoterlike DNA fragment was efficiently expressed in E. coli and P. putida. The toxin protein was efficiently synthesized in an insoluble form in both strains.

• Journal of Plant Biotechnology
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• v.46 no.4
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• pp.323-330
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• 2019

The high expression level of industrial and metabolically important proteins in plants can be achieved by plastid transformation. The CaIA vector, a Capsicum-specific vector harboring aadA (spectinomycin resistance), is a selectable marker controlled by the PsbA promoter, and the terminator is flanked by the trnA and trnI regions of the inverted repeat (IR) region of the plastid. The CaIA vector can introduce foreign genes into the IR region of the plastid genome. The biolistic method was used for chloroplast transformation in Scoparia dulcis with leaf explants followed by antibiotic selection on regeneration medium. Transplastomes were successfully screened, and the transformation efficiency of 3 transgenic lines from 25 bombarded leaf explants was determined. Transplastomic lines were evaluated by PCR and Southern blotting for the confirmation of aadA insertion and its integration into the chloroplast genome. Seeds collected from transplastomes were analyzed on spectinomycin medium with wild types to determine genetic stability. The increased chloroplast transformation efficiency (3 transplastomic lines from 25 bombarded explants) would be useful for expressing therapeutically and industrially important genes in Scoparia dulcis L.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.17 no.12
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• pp.1254-1261
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• 2007

The structure has several types of noise and booming noise of a vehicle is usually caused by the vibration of the vehicle's body transmitted from the engine through the mounting system. Vector synthesis analysis is performed to predict the booming noise when the characteristic of the engine mounting system is changed., i.e., when magnitudes and phases of vibratory forces after the mounts are altered. To use this method effectively, the concept of Multi-dimensional-analysis and Experimental Design are introduced to identify the contributions of each vibration sources and transmission paths to interior noise. It was used 3inputs/1output system and found the magnitudes and phases of the forces for minimizing the noise. Finally, the synthesized interior booming noise level is predicted by the vector synthesis diagram. It is shown that the vector synthesis method can be used to obtain the optimum design characteristic of the mounting system to control the interior booming noise of a vehicle.

• Journal of Biomedical Engineering Research
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• v.26 no.3
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• pp.129-132
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• 2005

It is one of the most important issues to determine a target brain image that gives a common coordinate system for a constructing population-based brain atlas. The purpose of this study is to provide a simple and reliable procedure that determines the target brain image among the group based on the inherent structural information of three-dimensional magnetic resonance (MR) images. It uses only 11 lines defined automatically as a feature vector representing structural variations based on the Talairach coordinate system. Average characteristic vector of the group and the difference vectors of each one from the average vector were obtained. Finally, the individual data that had the minimum difference vector was determined as the target. We determined the target brain image by both our algorithm and conventional visual inspection for 20 healthy young volunteers. Eighteen fiducial points were marked independently for each data to evaluate the similarity. Target brain image obtained by our algorithm showed the best result, and the visual inspection determined the second one. We concluded that our method could be used to determine an appropriate target brain image in constructing brain atlases such as disease-specific ones.

• Journal of Power System Engineering
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• v.4 no.4
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• pp.25-31
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• 2000

This paper represents the vector fields and three dimensional mean velocities in the X-Y plane of cone type swirl gas burner measured by using X-probe from the hot-wire anemometer system. This experiment is carried out at flowrate 350 and $450{\ell}/min$ respectively in the test section of subsonic wind tunnel. The vector plot shows that the maximum axial mean velocity component is focused in the narrow slits distributed radially on the edge of a cone type swirl burner, for that reason, there is some entrainment of ambient air in the outer region of the burner and the rotational flow can be shown in the inner region of the burner because mean velocity W is distributed about twice as large as mean velocity V due to inclined flow velocity ejecting from the swirl vanes of a cone type baffle plate of burner. Moreover, the mean velocities are largely distributed near the outer region of burner within $X/R{\fallingdotseq}1.5$, hence, the turbulent characteristics are anticipated to be distributed largely in the center of this region due to the large inclination of mean velocity and swirl effect.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.46 no.2
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• pp.66-72
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• 2009

In this paper, an efficient frequency offset synchronization structure for OFDM(Orthogonal Frequency Division Multiplexing) is proposed. Conventional CORDIC(Coordinate Rotation Digital Computer) algorithm for frequency offset synchronization utilizes two CORDIC hardware i.e., one is vector mode for phase estimation, the other is rotation mode for compensation. But proposed structure utilizes one CORDIC hardware and divider. Through simulation, it is shown that hardware implementation complexity is reduced compared with conventional structures. The Verilog-HDL coding and front-end chip implementation results for the proposed structure show 22.1% gate count reduction comparison with those of the conventional structure.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.45 no.5
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• pp.118-125
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• 2008

In this paper, an efficient frequency offset synchronization structure for OFDM(Orthogonal Frequency Division Multiplexing) is proposed. Conventional CORDIC(Coordinate Rotation Digital Computer) algorithm for frequency offset synchronization utilizes two CORDIC hardware i.e., one is vector mode for phase estimation, the other is rotation mode for compensation. But proposed structure utilizes one CORDIC hardware and divider. Through simulation, it is shown that hardware implementation complexity is reduced compared with conventional structures. The Verilog-HDL coding and front-end chip implementation results for the proposed structure show 22.1% gate count reduction comparison with those of the conventional structure.

• Journal of Power Electronics
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• v.4 no.2
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• pp.77-86
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• 2004

This paper presents a novel three-phase power module bridge type auxiliary resonant AC link snubber for the three-phase voltage-fed sinwave soft switching PWM inverter operating under specific instantaneous space voltage vector modulation. The operating principle of this resonant snubber is described for current source load model during one switching period, along with its design approach based on the simulation data. The performance evaluations of space vector modulation three-phase sinewave soft switching inverter with a new three-phase active auxiliary resonant AC link snubber are discussed as compared with those of three-phase voltage source-fed sinewave hard switching PWM inverter with a standard space voltage vector modulation strategy. The power loss analysis and conventional efficiency estimation of three-phase soft switching PWM inverter using ICBT modules are carried out including all the conduction power losses based upon the measured v-i characteristics of IGBT and its antiparallel diode as well as their switching losses.