• Korean Journal of Weed Science
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• v.9 no.1
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• pp.56-68
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• 1989

aA series of experiments were conducted to investigate the effect of plant growth regulator treatments on the growth and lateral root formation in soybean sprouts in order to establish the effective method of producing root-less or short-rooted soybean sprouts with larger diameter in the hypocotyl. Major results can be summarized as follows. 1. Soybean sprouts showed fairly uniform elongation rate from 3 to g days after imbibition with daily increase of 3.8cm. The speed of elongation of hypocotyl was reduced whereas that of root accelerated 7 days after imbibition. Lateral roots began to emerge fairly evenly from 5 to 9 days after imbibition with a daily increase of 4.4. 2. Auxins(IAA, IBA, NAA, 2,4-D) inhibited hypocotyl elongation and formation of lateral roots and increased hypocotyl diameter without influencing root length and hook diameter at higher concentrations. The dry weight of cotyledon was increased significantly as compared to that of hypocotyl and root. Among the tested auxins, 2, 4-D was the most effective. 3. BA and 4PU-30 significantly reduced elongation of hypocotyl and root and resulted in the biggest diameter of hypocotyl when treated at higher concentrations. The lowest effective concentration of BA to prevent the formation of larval gal roots was 12.5ppm. The formation of lateral roots could be completely prevented by BA and 4PU-30 treatment but kinetin, zeatin, zeatin riboside resulted in many lateral roots and increased thickness of soybean sprouts with little influence. Cotyledon deformation was found in soybean sprouts treated by 4PU-30. 4. 2, 4-D was the most effective for increasing the hypocotyl diameter while 4PU-30 was the most effective for reducing no. of lateral roots. 5. It can be concluded that among the plant growth regulators tested, BA was effective in reducing root length and increasing hypocotyl diameter. BA 12.5 ppm or 15 ppm may thus be the more practical for production of soybean sprouts. 6. ABA showed no significant effect of growth parameter, however ABA 25 ppm inhibited only no of lateral roots with little influence on the growth of seedling. 7. Ethephon inhibited the elongation of hypocotyl and root and increased hypocotyl diameter at higher concentrations. 8. The combined effect of cytokinins and ethephon was very similar to result of BA treatment alone. As the ethephon concentration increased, hypocotyl diameter and dry weight of cotyledon tended to increase.

• Applied Biological Chemistry
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• v.38 no.3
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• pp.202-206
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• 1995

In order to obtain the basic data for clarifing the mechanism of cold tolerance in crops, we analyzed various biochemical changes according to the Uniconazole treatment in Brassica seedling. Peroxidase activity in the root fraction of Brassica seedling was about 3 to 4 times higher than that in hypocotyl fraction, while catalase activity in those fractions showed opposite trend to the peroxidase activity. The content of hydrogen peroxide in root fraction was higher than that of hypocotyl fraction as being a reciprocal proportion with catalase activity. Especially in all fractions, peroxidase· activity of 'Sandongchae' (B. campestris) seedling, known as cold tolerant, was two-fold higher than that of cold sensitive rape(B. napus). The elongation rate of hypocotyl after germination was faster in B. napus than in B. campestris. The application of Uniconazole at 0.3 to 1.0 ppm to B. napus suppressed 43 to 46% of hypocotyl elongation and increased 65 to 73% of peroxidase activity in hypocotyl fraction. The shortening rate of hypocotyl length due to Uniconazole treatment was positively correlated with the increasing rate of peroxidase activity in hypocotyl fraction. Superoxide dismutase was not induced upon Uniconazole treatment and has only 3 isozymes in any fractions. Its activity was observed in the order of cotyledon>root>hypocotyl fraction.

• Journal of Environmental Science International
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• v.24 no.6
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• pp.755-762
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• 2015

The present study was conducted to develop seed treatment for the production of healthy and clean peanut sprout. Dry heat treatment of peanut seeds reduced the incidence of the rot. The seed treatment condition at $52^{\circ}C$ for 10 h. was the most efficient without inhibiting seed viability significantly. Seeds were dark cultured at $27^{\circ}C$ for up to 9 days. The treatment of Indole-B and gibberellic acid influenced germination, T50, fresh, dry weight, hypocotyl length, hypocotyl length diameter, root length, number of lateral root and epicotyl of peanut sprout. There were no differences in the germinability of peanut seeds between gibberellic acid treatment methods but higher fresh weight was observed in the GA3 solution spray after 2 hour water soaking. The general growth and lateral root development of peanut sprouts were suppressed by Indole-B which is used for inhibiting root formation and promoting hypocotyls. The treatment of gibberellic acid promoted hypocotyl elongation, but it did not influence on the growth of hypocotyls and root system.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.5
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• pp.526-534
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• 1996

This study was conducted to find out the effects of crude extracts from welsh onion, onion, chinese chives and garlic on the seedling growth of chrysanthemum, lettuce, rice, radish, chinese cabbage, cucumber and oriental melon. The crude extracts from Allium spp. inhibited the growth of chrysanthemum and lettuceseedlings at low concentration, the effect increased as the concentration grew higher, and especially in lettuce, the radicle growth was inhibited greater than the hypocotyl growth.The root extracts from welsh onion, chinese chives, and stem-leaf extracts from welsh onion, chinese chives and garlic significantly promoted the root growth of rice seedlings at 300∼700ppm and at 500∼1,000ppm, respectively, and the leaf sheath was elongated at low concentration of all extracts. The stem-leaf extracts from garlic and onion promoted the root and hypocotyl growth of chinese cabbage up to 2,000ppm or 3,000ppm. Root extracts from all Allium spp. promoted the elongation of chinese cabbage root up to 300∼700ppm, but they didn't influence its hypocotyl elongation. At low concentration of onion, garlic and chinese chives extracts, the growth of radish seedlings was highly promoted. The promotive effect, however, declined as the concentration increased. Only, extracts of chinese chives promoted the growth of radish seedling up to 5,000ppm. The stem-leaf extracts from onion and garlic promoted hypocotyl growth in pepper, up to 5,000ppm, but had little effect on other treatments. All of the extracts markedly promoted both root(main and lateral root) and hypocotyl growth in cucumber seedlings. A higher degree of promotion was made in the cucumber lateral root by onion and garlic extracts. The seedling growth of oriental melon was slightly increased by low concentration, but high concentration inhibited the root and hypocotyl growth.

• Journal of Photoscience
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• v.9 no.2
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• pp.391-393
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• 2002

Effects of illumination with xenon or sulfur lamp on growth of tomato seedlings were studied. Results showed no obvious differences in net photosynthetic rate and apparent quantum efficiency. In comparison with xenon lamp, illumination with sulfur lamp slightly reduced ChI a and ChI b content in leaves, relatively shortened epicotyl and hypocotyl length, but significantly increased anthocyanin content of leaves and promoted root growth of tomato seedling plants.

• Journal of Plant Biology
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• v.35 no.2
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• pp.107-116
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• 1992

The roles of RNA- and protein-synthesis and $H^{+}$ excretion in 1AA ($10\;\mu\textrm{M}$)-induced elongation were investigated using abraded hypocotyl segments of sunflower (Helianthus annuus L.). The response of elongation initiated about 13 min after IAA treatment. Removal of cuticle, acting as diffusion barrier for inhibitors, by mechanical abrasion of hypocotyl segments enhanced the effect of inhibitors markedly, but the degree of abrasion for the saturated effect of inhibition was different among inhibitors. The elongation induced by 1M was completely inhibited when cycloheximide ($10\;\mu\textrm{M}$) was applied to abraded hypocotyl segments as shortly as 4 min before the onset of the growth response (= 10 min after administration of IAA). Cordycepin ($200\;\mu\textrm{M}$) prevented completely 1AA-induced elongation when applied as shortly as 19 min before the onset of the growth response (=5 min before administration of 1AA). Vanadate (1 mM) inhibited both lAA-induced elongation and medium acidification via lAA-induced $H^{+}$ excretion to apoplast. Cycloheximide and cordycepin also prevented lAA-induced $H^{+}$ excretion strongly. However, inhibition by cycloheximide of lAA-induced elongation was not alleviated by acidifying the cell wall to pH 4.5. The results indicate that, a few minutes before the initiation of growih, protein synthesis is demanded for the initiation of 1AA-induced elongation and the $H^{+}$ excretion to cell wall, and that the H+ excretion, even though it may be necessary for elongation, does not seem to bring about acid growth simply through acidifying cell wall.l wall.

• Korean Journal of Soil Science and Fertilizer
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• v.36 no.6
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• pp.429-436
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• 2003

The relationship between seeding depth and apical hook opening was investigated in the hypocotyl hook of soybean (Glycine max Merr., cv. Hwanggeum). Seeds were sawn in different depths (2.5, 5.0, 7.5, and 10.0 cm). The hook opening was slowly progressed with seeding depth. Hook angle opening velocity was negatively correlated with hypocotyl growth at the significant level of P<0.01. It was also clearly observed that seeding depth was positively correlated with hypocotyl growth, suggesting the induction of hypocotyl growth by deep sawing. Futhermore, the contents of fructose and glucose in hypocotyls were about higher than in cotyledons. Both sugars in hypocotyls were highest at the emergence stage. After emergence, their levels were obviously reduced. Total soluble sugar contents continuously retained in cotyledons which were grown at 2.5 and 5.0 cm seeding depths whereas the contents in cotyledons of deep sawn soybean were extremely lowered. It seemed that sugars were actively used to cell construction during the hypocotyl elongation. The results demonstrated that apical hook opening is closely related with light signal after emergence. It implied that the delay of hook opening in deep sawn seeds was resulted from hypocotyl growth in darkness. We suggest that apical hook opening is progressed in sucrose catabolism by light.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.35 no.1
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• pp.58-64
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• 1990

This study was conducted to investigate the interaction effect of a new plant growth regulator brassinolide and auxins using several bioassay methods such as rice lamina inclination test, oat coleoptile segment growth test and radish hypocotyl elongation test. For rice lamina inclination test, the antagonistic response showed at low concentration combinations of homobrassinolide and auxins (IAA and 2,4-D), while the combinations of high concentration responsed mostly synergistic or additive effects, IAA was generally higher than 2,4-D for combination effect with homobrassinolide. For oat coleoptile segment growth test, the synergistic effect showed at IAA concentrations less than 0.1ppm while additive response exhibited above 0.3ppm regardless of homobrassinolide concentrations. In radish hypocotyl elongation test, the interaction response varied with cotyledon. The sections removed cotyledon showed mostly antagonistic effects, except for combination of homobrassinolide with IAA 3ppm. Interaction responses were Quite different from bioassay methods, particularly using experimental materials: antagonistic responses were usually shown at the section that attached growing point while these for sections that removed growing point were responded synergistic or additive effect due to unstable endogenous hormones.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.4
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• pp.331-338
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• 1994

The experiment was conducted to determine if seed size and seed coat color of soybean might be effective in water uptake and cotyledon damage after imbibition, and hypocotyl elongation. Eight soybean cultivars were separated into two classes of large and small seed based on seed weight, and each class included two cultivars with yellow and black seed color, respectively. Small seed size group was superior in water uptake by seed for 24 hour in imbibition at $25^{\circ}C$ , but its differences decreased as soaking time increased. Small seed cultivars germinated faster and had better germination rate than large ones. However, cultivars with black seed coat showed more slow water uptake at initial time and faster germination than yellow seed, but in 24 hour after imbibition, cultivars with black seed coat had higher water uptake rate than yellow seeds. Small seed cultivar group showed no cotyledon damage in imbibition for 24 hour while large seed cultivars were damaged 78% of cotyledon, and black seed showed low cotyledon damage compared to yellow seed. Hypocotyl length was shorter in large seed rather than in small seed, but hypocotyl thickness in large seed was more thick than in small seed. In correlation coefficients, seed coat rate, embryo rate exhibited significantly negative association with seed weight, and the correlation of seed weight with water uptake in 3 hour after soaking was significantly negative, but in 24 hour showed positive correlation.

• Horticultural Science & Technology
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• v.16 no.3
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• pp.344-346
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• 1998

Early emergence of watermelon seeds was markedly enhanced by SMP treatment though the final emergence percentage was not significantly influenced. Mixing ratios of 10:1:5 and 10:1:6 (seed : Micro-Cel E : water by weight) were found to be most effective in promoting the early emergence of watermelon seeds primed for 72 hrs at $25^{\circ}C$. Even though the hypocotyl elongation was slightly promoted by SMP treatment, the hypocotyl diameter remained uninfluenced. Among the cultivars tested, 'House-Ilpum' and 'Surowang' showed the best response to the SMP treatment.