• Journal of dental hygiene science
• /
• v.3 no.1
• /
• pp.39-44
• /
• 2003

This study was to investigate the antibacterial effect of Schizandrae fructus and Evodiae fructus extracts on S. mutans growth. The antibacterial effect of these extracts on S. mutans was good at Schizandrae fructus extract than the Evodiae fructus extract. The antibacterial activities of these extracts were measured 8.5 mm and 6.5 mm at 20 mg/ml concentration, respectively. The growth of S. mutans in control medium was the highest at 8 hrs, while the media of Schizandrae fructus and Evodiae fructus extracts added-medium(2 mg/ml) showed maximum growth at 16hrs. The pH values of the control medium was 5.08 at 8 hrs, but the media supplemented with Schizandrae fructus and Evodiae fructus extracts were 6.00, and 5.36 at 8 hrs, respectively. The amounts of total carbohydrate for the control media was 0.81 mg/ml at 8 hrs, but the media supplemented with Schizandrae and Evodiae fructus extracts were 1.32 mg/ml and 1.88 mg/ml at 8 hrs, respectively. In the change of culture medium proteins, the control culture broth and cultures supplemented with Schizandrae fructus and Evodiae fructus extracts were 8.39 mg/ml, 8.59 mg/ml and 9.97 mg/ml at 8 hrs, respectively. The S. mutans polysaccharide contents of the control medium and the media supplemented with Schizandrae and Evodiae fructus extracts were 300 mg/100 ml, 240 mg/100 ml and 280 mg/100 ml at 8 hrs, respectively.

• Journal of dental hygiene science
• /
• v.16 no.6
• /
• pp.401-408
• /
• 2016

Periodontal disease is one of the major dental diseases. Currently, various methods are used for healing and successful regeneration of periodontal tissue damaged by periodontal disease. The periodontal ligament and alveolar bone have received considerable interest for use in periodontal tissue regeneration and induction. However, as the functions of the factors required for tooth attachment and key regulatory factors for periodontal tissue regeneration in the cementum have recently been identified, interest in cementum formation and regeneration has increased. Dental cementum forms in the late phase of tooth development because of the reciprocal regulatory interaction between cervical loop epithelial cells and surrounding mesenchymal cells, which is regulated by various gene signaling networks. Many attempts have been made to understand the regulatory factors and cellular and molecular mechanisms associated with new cementum formation. In this paper, we reviewed the study outcomes to date on the regulatory factors that induce cementum formation and regeneration, focusing on understanding the roles and functions of Wnt signaling in the regulation of cementum formation. In addition, we aimed to obtain information on the useful reciprocal regulatory factors that mediate cementum formation and regeneration through a series of molecular mechanisms.

• Korean Journal of Health Education and Promotion
• /
• v.22 no.1
• /
• pp.73-86
• /
• 2005

This research was performed to be used as basic data for educational program development related to smoking prohibition guidance by fording out the relevance among variables of dental hygienists' knowledge, attitude and practice on the smoking prohibition guidance. The formalized research paper which had been used to nurses was revised with a few complements and accomplished through a visit. Both-test and dispersion analyses were performed and a post test was followed. Relevance analysis was fulfilled for the relevance among the variables and the research results are as followed. 1. Likert 3 and grade 2.14 in the knowledge on smoking prohibition guidance of dental hygienists; These results are statistically similar since the higher academic careers are based, the higher grades are obtained. 2. Likert 5 and grade 3.14 in the attitude on hygienists' smoking prohibition guidance activities. 3. High awareness on the importance of non-smoking in case there is no smoker among family. 4. Likert 5 and grade 3.16 in the practice of smoking prohibition guidance activities. 5. Similar relevance among the knowledge, attitude and practice; the higher knowledge, the more positive attitude and the more positive attitude, the more practice.

• Safety and Health at Work
• /
• v.8 no.2
• /
• pp.156-161
• /
• 2017

Background: Occupational Health and Safety Management Systems are becoming more widespread in organizations. Consequently, their effectiveness has become a core topic for researchers. This paper evaluates the performance of the Occupational Health and Safety Assessment Series 18001 specification in certified companies in Iran. Methods: The evaluation is based on a comparison of specific criteria and indictors related to occupational health and safety management practices in three certified and three noncertified companies. Results: Findings indicate that the performance of certified companies with respect to occupational health and safety management practices is significantly better than that of noncertified companies. Conclusion: Occupational Health and Safety Assessment Series 18001-certified companies have a better level of occupational health and safety; this supports the argument that Occupational Health and Safety Management Systems play an important strategic role in health and safety in the workplace.

• Asian Pacific Journal of Cancer Prevention
• /
• v.15 no.20
• /
• pp.8809-8814
• /
• 2014

The objective of this paper is to present the implementation and process evaluation of the first computer tailored program for smoking cessation among Romanian smokers. The program targeted adult smokers who declared the intention to quit smoking in the next six months. The intervention consisted of a letter tailored to several respondent characteristics: gender, cognitive variables (attitude, social influence, and self-efficacy), intention to quit smoking, goal and relapse prevention strategies (action and coping plans), and smoking behaviour. The first 80 participants entered into the program filled in a process evaluation questionnaire one month after the intervention. The results of our study confirmed that the participants had read and remembered the letter. Moreover, new for Romania, this approach was positively appreciated by the participant and the score received for the tailored letter was high. The opinions of the participants confirmed that the tailored letter provided information that was useful, trustworthy and relevant for the individual. At the same time, the participants appreciated the polite, easy to understand content of the letter. These data underlined the premises for continuing the program and for using the information and communication technologies for healthy lifestyle promotion among Romanian population.

• Journal of Korean society of Dental Hygiene
• /
• v.18 no.1
• /
• pp.9-18
• /
• 2018

Objectives: Pleurotus eryngii is used both for edible and medicinal purposes, and has a physiological activity. The purpose of this study is to investigate the antibacterial effect of Pleurotus eryngii against six oral pathogens (Staphylococcus aureus, Streptococcus criceti, Streptococcus mutans, Streptococcus ratti, Streptococcus sobrinus, and Actinomyces viscosus). Methods: The antibacterial activities of various extracts of Pleurotus eryngii were examined by disc diffusion assay and minimum inhibitory concentration (MIC). The disc diffusion assay was performed by putting a paper disc soaked in extracts on plates inoculated bacterial cultures. The MIC of these extracts was determined by using a broth microdilution assay at a concentration ranging between 0.03 mg/ml to 15.00 mg/ml. The growth inhibition effect of extracts was measured at 600 nm for 24 hrs. Results: The antibacterial activity was confirmed against all six tested bacteria at Pleurotus eryngii ethyl acetate extract by the disc diffusion method. Acetone extract showed the antibacterial activity only against 4 strains containing Streptococcus criceti, Streptococcus mutans, Streptococcus ratti, and Actinomyces viscosus. In ethanol extract, no activity was observed against other strains except Staphylococcus aureus. MIC values of ethyl acetate extract were the same, 7.50 mg/ml in all tested bacteria. Conclusions: Pleurotus eryngii exhibited the antibacterial activity against oral pathogens (Staphylococcus aureus, Streptococcus criceti, Streptococcus mutans, Streptococcus ratti, Streptococcus sobrinus, and Actinomyces viscosus). Thus, Pleurotus eryngii may be considered as a natural antibacterial agent for treatment of dental diseases.

• Journal of Korean society of Dental Hygiene
• /
• v.10 no.6
• /
• pp.1095-1105
• /
• 2010

Objectives : This paper is designed to help the public prevent their future dental caries, based on the studies of xylitol about it's efficacy and effectiveness, and its appropriate usage. Methods : This study of xylitol on the basis of existing research papers and literature is not only to learn about the mechanism of what xylitol acts, but also to search the effectiveness of incompleteness of xylitol-containing foods, bacterial infection between xylitol and mother-baby, and the side-effects of xylitol. Results : 1. Xylitol's continuous intake of teeth effects the decrease of calculus amount, cleanness of teeth, as well as the remineralization process. The xylitol effect varies depending on the weight of xylitol, which has no effect on root caries. 2. Mouth gargle solution is higher resistant against acids; recalcification is significantly reduced; and remineralization effect represents. 3. It is reported that regular consumption of xylitol by mothers just during two years after birth has significantly decreased the spread possibility of mutans streptococcei. 4. The reduction of dental caries by xylitol is happened by its restraining the growth of bacteria resource caries, with their presence in amount of uncontrolled growth. 5. When a small amount of eating xylitol is absorbed slowly into gastrointestinal tract, being followed by glucose metabolism in the liver, while its excessive amount of eating can cause gastrointestinal problems. Conclusion : Dental hygienists should be required to provide dental information to the public on the basis of academic knowledge. In situation that one is not yet sure of xylitol on solid and multifaceted researches, there must be needed more accurate and detailed reviews on the side-effects of xylitol and the overall impact on the oral environments.

• Journal of dental hygiene science
• /
• v.21 no.1
• /
• pp.45-51
• /
• 2021

Background: Periodontal disease, also known as gum disease, is a major dental inflammatory disease with a very high prevalence; it is the main cause of tooth loss. Therefore, diagnostic biomarkers that can monitor gum inflammation are important for oral healthcare. Since the gingival crevicular fluid (GCF) adequately reflects changes in the periodontal environment, they have become a target for the development of effective diagnostic biomarkers for periodontitis. In the present study, the level of the target molecules suggested as diagnostic biomarkers for periodontitis were analyzed in GCF samples collected from healthy individuals and periodontitis patients. In addition, useful targets for the diagnosis of periodontitis were evaluated. Methods: GCF samples were collected from healthy individuals and periodontitis patients using absorbent paper points. SDS-PAGE and Coomassie staining were performed for protein analysis. The protein concentrations of GCF specimens were determined using the Bradford method. The levels of the target molecules appropriate for diagnosing periodontal disease were measured by ELISA, according to the manufacturer's protocol. Results: The protein concentration of GCF collected from periodontitis patients was 3.72 fold higher than that in an equal volume of GCF collected from healthy individuals. ELISA analysis showed that the level of interukin-6 (IL-6), IL-8, metalloproteinases 2 (MMP-2), MMP-9, tumor necrosis factor-alpha (TNF-α), azurocidin, and odontogenic ameloblast-associated protein (ODAM) were higher in the GCF samples from the periodontitis patients than in those from the healthy individuals. However, the level of IL-6 and TNF-α were relatively low (> 5 pg/ml). The prostaglandin E2 (PGE2) levels were not significantly different between the two GCF samples. Conclusion: These results indicate that IL-8, MMP-2, MMP-9, azurocidin, and ODAM are potentially useful diagnostic biomarkers for periodontitis; combining multiple biomarkers will improve the diagnostic accuracy of periodontitis.

• Asian Pacific Journal of Cancer Prevention
• /
• v.13 no.12
• /
• pp.6327-6332
• /
• 2012

Esophageal cancer is a common malignant tumor occurring in human esophageal epithelial tissue. The primary purpose of this paper was to define the effects of ${\beta}$-carotene and 1,25-dihydroxyvitamin $D_3$, alone and in combination, on cell proliferation, cell cycle and apoptosis of human esophageal cancer EC9706 cells. Treatment with different concentrations of ${\beta}$-carotene and/or 1,25-dihydroxyvitamin $D_3$. MTT assay showed that ${\beta}$-carotene and 1,25-dihydroxyvitamin $D_3$ significantly inhibited proliferation of EC9706 cells in a dose- and time-dependent manner. Further studies also demonstrated that ${\beta}$-carotene alone or 1,25-dihydroxyvitamin $D_3$ alone caused a marked increase on the induction of apoptosis in EC9706 cells. The percentage of G0/G1-phase cells significantly increased on addition of 1,25-dihydroxyvitamin $D_3$ alone, but there were no significant changes with ${\beta}$-carotene alone. These two agents in combination synergistically inhibited cell growth and induced apoptosis. Therefore, our results indicate that ${\beta}$-carotene and 1,25-dihydroxyvitamin $D_3$ in combination may provide a novel strategy for preventing and treating esophageal cancer.

• Journal of Food Hygiene and Safety
• /
• v.8 no.1
• /
• pp.1-8
• /
• 1993

육류중에 잔류하는 항생물질 및 항균제를 검출하기 위해 본 실험에서는 3종의 균주 Bacillus subtilis ATCC 6633, Micrococcus luteus ATCC 9341, BAcillus cereus var. mycoides ATCC 11778을 사용하여 실험하였다. 시료의 clean-up은 항생·항균제의 물리화학적 성질을 고려하여 우선 McIlvaine buffer를 가하여 homogenize하고 hexane으로 defatting 시킨 후, chloroform으로 추출한 액과 Sep-Pak C18과 Bakerbond SPE carboxylic acid column에 흡착시킨 후 추출한 액을 각각 시험용액 A, B, C,로 하였다. 각 test solution을 paper disk를 사용하여 함균 배지에 올려놓고 overnight culture 후 inhibition pattern을 통해 여러 종류의 항생·항균제를 계통적으로 검출하였다. 본 실험에서 macrolide계와 tetracycline계 등은 0.1ppm 이하의 detection limit을 보였으며, penicillinrP는 0.001ppm이하의 높은 detection limit을 나타내므로서 시료중에 잔류하는 극미량까지도 검출할 수 있었다. 본 방법은 식육중의 잔류 항생·항균제를 동시에 간단하게 계통적으로 분류하는데 있어 좋은 방법이라고 생각되며 항생·항균제의 체계적인 1차 screening 수단으로서 유용한 방법이라 사료된다.