• 대한후두음성언어의학회지
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• 제22권2호
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• pp.119-125
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• 2011

Background and Objectives : Temporary or permanent vocal paralysis can be occurred after head and neck surgery such as thyroid cancer, esophageal resection, and chest operation including lung parenchymal resection, due to a vagus or recurrent laryngeal nerve injury. The authors aimed to determine the clinical efficacy of using Calcium-Hydroxyapatite (CaHA) for permanent unilateral vocal cord palsy patients. Materials and Method : Between July 2008 to July 2010, among patients with chief complain of hoarseness and aspiration, only who were diagnosed as unilateral vocal cord palsy under laryngoscopy, were selected. The patients included 3 females and 13 males age range between 29 to 79 and average age was 60 years old. Results : The hoarseness range were $8.94{\pm}0.77$, $4.63{\pm}1.02$, $4.31{\pm}1.30$ statistically showing significant postoperative improve at preoperative, 1 week and 3 months. Also aspiration were $7.44{\pm}2.48$, $3.63{\pm}1.82$, $3.19{\pm}1.91$ statistically improved during the same period. The result of voice analysis showed that the frequency range shows decrease at 1 week and 3 months after the injection compared to that of the preoperative result in both male and female group (Male: $161.63{\pm}32.78$ Hz, $139.13{\pm}30.63$ Hz, $146.67{\pm}34.20$ Hz ; Female: $244.62{\pm}26.62$ Hz, $244.91{\pm}42.03$ Hz, $237.50{\pm}38.95$ Hz). The Maximal phonation time were $2.75{\pm}1.06$ (sec), $8.88{\pm}3.46$ (sec), $8.44{\pm}3.71$ (sec) statistically showing significant postoperative improve at preoperative, 1 week and 3 months. Conclusion : Injection laryngoplasty with CaHA in unilateral vocal cord paralysis is very safe and efficient procedure to improve a voice disorder, a swallowing difficulty, and a quality of life for those patients with a sacrificed RLN, a cancer invasion of the nerve, and a prolonged vocal cord paralysis which is more than six to twelve months.

• 치위생과학회지
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• 제8권4호
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• pp.367-373
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• 2008

천연물 황금으로부터 여러 유기용매 추출물을 얻어 치아우식증 원인균인 S. mutans 에 대한 항균활성, 배지 내 환경변화, 그리고 부착억제에 대해 알아보았다. 1. 구강 질병인 치아우식증의 대표적 원인균 S. mutans에 대한 황금 ethyl acetate 추출물 (IPK-3)의 최소억제농도는 125 mg/ml 이었다. 2. 황금 IPK-3 추출물 50 mg/ml의 농도로 S. mutans의 배지 내 투여하였을 때 균의 생장 량과 최대 생장시간은 대조군보다 지연되었다. 3. 황금 IPK-3 추출물 50 mg/ml의 농도로 S. mutans의 배지 내 투여하였을 때, pH 변화는 대조군은 18시간에서 pH 5.63으로 급격한 변화를 보였으며 황금 IPK-3 추출물이 50 mg/ml의 배지에서는 6.50이상을 유지하여 pH가 변화가 없었다. 4. 황금 IPK-3 추출물을 첨가한 배지 내 탄수화물, 단백질 및 균체 외 다당류의 변화는 대조군에 비해 매우 낮게 생산되었다. 5. 균체를 형광염료(DAPI)로 염색하여 S-HA 부착억제을 확인한 결과, 대조군은 시간이 경과함에 따라 S.mutans의 hydroxyapatite(HA)에 부착 정도가 증가되었으나, 황금 IPK-3 추출물이 첨가된 시험군에서는 S-HA에 균의 부착이 매우 적었다. 이같이 황금의 IPK-3 (ethyl acetate 추출물)이 치아우식증의 원인균인 S. mutans 에 대한 항균활성효과 뿐만아니라 hydroxyapatite에 부착 억제 효과도 있었다.

• 한국진공학회지
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• 제7권s1호
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• pp.50-56
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• 1998

Ion beam assisted deposition (IBAD) was successfully used to produce a dense and ultra-adherent Hydroxyapatite (HA) film on titanium alloy and alumina. Recently it is also proved that the HA coatings on alumina substrate treated with 20 ppm $AgNO_3$ had the structure of $(Ag, Ca)_10(PO_4)6(OH)_2$, which exhibited excellent antimicrobial effects. The present paper aims to morphlogically characterize the adhesion of macrophages on newly developed Ag-HA coated alumina and Ti6A14V substrates and to evaluate the biocompatibility of the coatings in vitro. It can be found that the cell number on alumina of the concentration of $AgNO_3$ in the treatment, the cell number on Ag-HA coatings decreased. Up to 20 ppm $AgNO_3$ by Ag-treatment, the morphological development of the cells on Ag-HA coating was similar to that of the cells on HA coating, suggesting the biotolerance of the Ag-HA coatings.

• 한국임상수의학회지
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• 제27권4호
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• pp.325-329
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• 2010

본 연구에서는 hydroxyapatite/poly $\varepsilon$-caprolactone composite (HA/PCL) 지지체와 matrigel을 랫드의 두개골 결손부 모델에 함께 이식 시의 골형성 정도를 평가하였다. 두개골결손부는 Sprague Dawley rat (n = 18)에서 수술적으로 형성하였으며 실험군은 Matrigel과 함께 HA/PCL 지지체를 이식한 군(M-HA/PCL group, n = 6)과 HA/PCL 지지체단독이식군(HA/PCL group, n = 6)으로 나누었고 대조군(CD group, n = 6)에는 아무 것도 이식하지 않았다. 수술 4주 후, 골형성은 방사선촬영, micro CT 및 조직검사를 통해 평가되었다. 방사선상에서 CD군의 골형성은 관찰되지 않았으나 HA/PCL과 M-HA/PCL군에서는 관찰되었고 골과 유사한 방사선비투과성이 M-HA/PCL군에서 더 많이 관찰되었다. Micro CT 평가에서 골부피는 HA/PCL군보다 M-HA/PCL군에서 더 높았으나 두 군 사이의 유의적 차이는 관찰할 수 없었다. 그러나 골밀도에서는 HA/PCL군보다 M-HA/PCL군이 더 유의적으로 높음을 확인할 수 있었다(p < 0.05). 조직학적 검사에서는 CD군에서 새로운 골은 원래 존재하던 골로부터만 형성되었으며 두개골결손부 내의 골형성은 보이지 않았다. HA/PCL군에서 새로운 골형성은 원래 존재하던 골로부터만 유래되었으나 M-HA/PCL군은 가장 많은 골형성을 보여주었으며 새로운 골이 원래 존재하던 골과 HA/PCL지지체 주변에서도 관찰되었다. 이러한 결과로 미루어볼 때 HA/PCL 지지체와 matrigel을 함께 사용하는 것이 골의 임계결손부에서 골형성을 증대시키는 효과적인 방법이 될 수 있을 것으로 생각된다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제38권
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• pp.26.1-26.6
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• 2016

Background: Xenologous or synthetic graft materials are commonly used as an alternative for autografts for guided bone regeneration. The purpose of this study was to evaluate effectiveness of carbonate apatite on the critical-size bone defect of rat's calvarium. Methods: Thirty-six critical-size defects were created on 18 adult male Sprague-Dawley rat calvaria under general anesthesia. Calvarial bones were grinded with 8 mm in daimeter bilaterally and then filled with (1) no grafts (control, n = 10 defects), (2) bovine bone mineral (Bio-$Oss^{(R)}$, Geistlich Pharma Ag. Swiss, n = 11 defects), and (3) hydroxyapatite ($Bongros^{(R)}$, Bio@ Inc., Seongnam, Korea, n = 15 defects). At 4 and 8 weeks after surgery, the rats were sacrificed and all samples were processed for histological and histomorphometric analysis. Results: At 4 weeks after surgery, group 3 ($42.90{\pm}9.33%$) showed a significant difference (p < 0.05) compared to the control ($30.50{\pm}6.05%$) and group 2 ($28.53{\pm}8.62%$). At 8 weeks after surgery, group 1 ($50.21{\pm}6.23%$), group 2 ($54.12{\pm}10.54%$), and group 3 ($50.92{\pm}6.05%$) showed no significant difference in the new bone formation. Conclusions: $Bongros^{(R)}$-HA was thought to be the available material for regenerating the new bone formation.

• Journal of Korean Neurosurgical Society
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• 제59권6호
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• pp.604-609
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• 2016

Objective : The aim of this study was to compare the clinical and radiological outcomes associated with the use of hydroxyapatite (HA) spacer and allogeneic bone (AB) spacer in laminoplasty. Methods : From January 2006 to July 2014, 79 patients with cervical spondylotic myelopathy or ossification of the posterior longitudinal ligament underwent cervical laminoplasty. The radiologic parameters were obtained from plain radiography and three-dimensional computed tomography. All images were taken before and after surgery. Cervical lordosis, spinal canal dimension, fusion between lamina and spacer, and resorption of spacer were checked. Clinical outcomes were assessed using visual analog scale and Japanese Orthopedic Association. Results : Double-door laminoplasty was performed on 280 levels : 182 in the HA group and 98 in the AB group. The mean follow-up was 23.1 months (range : 4-69 months). Similar fusion rates were found in these groups (p=0.3). The resorption rate between lamina and spacer was lower in the HA group (p<0.001). During the immediate postoperative period, the canal dimension of both groups increased compared with the results in the preoperative period. However, the canal dimension of the AB group decreased over time compared with that of the HA group (p<0.001). Conclusion : Double-door laminoplasty improved the clinical outcomes of both groups. However, the spinal canal dimension in the AB group showed a greater degree of reduction than in the HA group at the final postoperative follow-up. Therefore, we suggest that surgeons consider the use of larger-sized AB spacers in double-door laminoplasties.

• 대한핵의학회:학술대회논문집
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• 대한핵의학회 1999년도 제38차 추계학술대회
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• pp.42.2-42.2
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• 1999

• 한국방사선학회논문지
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• 제14권5호
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• pp.537-543
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• 2020

현대사회의 노인 인구는 첨단 의료기술과 최소 침습 수술로 인해 빠르게 성장하고 있다. 따라서 의료기기를 사용하는 경향이 증가하기 때문에 병원성 감염이 우려된다. 따라서 현대 의학의 최우선 목표는 감염예방이다. 최근 3D 프린팅을 이용하여 환자 맞춤형 임플란트 이식술이 늘고 있다. 대표적 재료로 이용되는 것이 수산화인회석이다. 현재 HA 디스크 멸균을 위한 흡수선량 기준이 없으므로, HA 디스크 표면에 오염된 대장균과 충치균을 감마선 코발트와 선형 가속기를 이용하여 각 흡수선량 0, 0.5, 1.0, 3.0, 5.0 kGy로 조사 후, 십진 희석법으로 검체에서 균 수를 측정하였다. 멸균 후 대장균, 충치균의 생존 비교분석을 위해 비모수 검정법을 시행하였으며, 그 결과 대장균은 1 kGy 이상, 충치균은 3 kGy 이상에서 멸균되었다. 방사선 멸균 흡수선량 권고사항보다 낮은 수치에서 멸균을 시행하는 것도 가능할 것으로 생각된다.

• 대한후두음성언어의학회지
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• 제29권2호
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• pp.83-86
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• 2018

Background and Objectives : This study aimed to evaluate efficacy and complication of injection laryngoplasty using calcium hydroxyapatite (CaHA) for unilateral vocal fold paralysis in Asian. Materials and Methods : A prospective study was conducted on the adult patients with unilateral vocal fold paralysis from May 2015 through January 2016. Injection laryngoplasty was performed by one laryngologist. All patients underwent prospective voice evaluation using the subjective and objective comprehensive battery of assessments, before the procedure and after the procedure at 3 months, and 6 months. Results : A total of 7 patients (5 males and 2 females) were included in this study. VHI-10 was significantly decreased after injection laryngoplasty, at postoperative 6 months (p=0.031), while VAS score and MDADI showed no difference. GRBAS scale gradually decreased in 3 months and 6 months follow-up without statistical significance. Acoustic analysis revealed that jitter, shimmer, and noise-to-harmonic ratio continuously decreased from the baseline at 3 months and 6 months, although statistical significance was not attained. In the aerodynamic analysis, maximal phonation time was gradually increased at 3months and 6 months with significant difference (p=0.016, 0.031, respectively). There was no side effect associated with the procedure. Conclusion : CaHA can be safely used in Asian patients and the onset of maximal efficacy seems to be slow than other studies with Caucasian patients.

• Composites Research
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• 제21권3호
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• pp.18-23
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• 2008

쾌속 조형(Rapid Prototyping; RP) 기술은 다양한 분야에서 활용되고 있다. 본 연구에서는 RP 기술을 이용한 나노복합재 적층장치(Nano Composite Deposition System, NCDS)를 사용하여 이식 가능한 약물전달시스템을 제작하였다. 약물전달시스템 복합재는 약물 입자로 5-fluorouracil (5-FU)를 사용하였으며, 생분해 고분자 매트릭스로 PLGA85/15를 사용하였다. 제작된 약물전달시스템은 넓은 표면적을 가질 수 있도록 지지체(scaffold) 형상으로 제작되었으며, in vitro 환경에서의 약물방출실험이 수행되었다. 약물방출제어를 위하여 생체적합재료인 수산화아파타이트(Hydroxyapatite, HA)를 약물-고분자 복합재에 첨가하였다. 약 50일간의 방출실험을 통하여 약물방출의 가능성을 보임을 확인하였다.