• 제목/요약/키워드: hydrophobic peptide

검색결과 93건 처리시간 0.024초

Plastein 반응에 의한 고온조리 어육추출물의 기능성 개선 (Improvement of Functional Properties of Extracts from Hydrothermal Cooked Fish Meat by Plastein Reaction)

  • 이근태;박성민;이상호;류홍수
    • 한국식품영양과학회지
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    • 제27권1호
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    • pp.93-101
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    • 1998
  • In order to improve the functional properties of several fish meat extracts as an alternate protein source, theri basic plastein reactions were evaluated. The UV absorption at 270 and 290 nm indicated that plasteins had higher amount of hydrophobic peptide or amino acid than the fish meat extracts. The water solubilities of the extracts were reduced at acidic pH. Values for the emulsifying capacity of the extracts and plasteins were over 30% although the latter showed the higher ones than the former. The osmolalities of the extracts at 1.0% concentration were 39(loach), 33(bastard halibut), 30(jacopever) and 24(crucan carp) milliosmole. Generally the slightly higher osmolalities were noted in the plasteins to be compared with the extracts. Both the extracts and plasteins exhibited a higher antioxidative effect than tocopherol. The hydrophobic amino acid which had been introduced at plastein reaction attributed the stronger antionxidative effect of its product than the extracts.

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Proteomic Analysis of the Hydrophobic Fraction of Mesenchymal Stem Cells Derived from Human Umbilical Cord Blood

  • Jeong, Ju Ah;Lee, Yoon;Lee, Woobok;Jung, Sangwon;Lee, Dong-Seong;Jeong, Namcheol;Lee, Hyun Soo;Bae, Yongsoo;Jeon, Choon-Ju;Kim, Hoeon
    • Molecules and Cells
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    • 제22권1호
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    • pp.36-43
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    • 2006
  • Mesenchymal stem cells (MSCs) are promising candidates for cell therapy and tissue engineering, but their application has been impeded by lack of knowledge of their core biological properties. In order to identify MSC-specific proteins, the hydrophobic protein fraction was individually prepared from two different umbilical cord blood (UCB)-derived MSC populations; these were then subjected to two-dimensional (2D) gel electrophoresis and peptide mass fingerprinting matrix-assisted laser desorption/ionization (MALDI)-time of flight (TOF)-mass spectrometry (MS). Although the 2D gel patterns differed somewhat between the two samples, computer-assisted image analysis identified shared protein spots. 35 spots were reliably identified corresponding to 32 different proteins, many of which were chaperones. Based on their primary sub-cellular locations the proteins could be grouped into 6 categories: extracellular, cell surface, endoplasmic reticular, mitochondrial, cytoplasmic and cytoskeletal proteins. This map of the water-insoluble proteome may provide valuable insights into the biology of the cell surface and other compartments of human MSCs.

Pseudomonas aeruginosa가 생산하는 biosurfactant의 분리 및 특성 (Purification and Properties of Biosurfactant from Pseudomonas aeruginosa KK-7)

  • 김대원;김민주;강상모
    • 한국미생물·생명공학회지
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    • 제23권3호
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    • pp.337-345
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    • 1995
  • Biosurfactants produced by Pseudomonas aeroginosa KK-7 were purified and their properties were studied. The bacterial surfactant was seperated into two sorts of biosufactants (Type I, 11) by silica gel column chromatograpgy. On the basis of physiochemical analysis, Type I was found to be mixture of two glycolipids with M.W. 800, and Type II was peptide with M.W. 1300. The Type 11 biosurfactant was compose of glutamic acid, proline, glycine, leucine, histidine. The crude extract was used to dertermine some properties as a surfactant. The biosurfactant had the properties as stronger emulsification agent and a stronger stabilizing agent emulsion than any other surfactants tested.

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Reactivity of Functional Food Substance in terms of Structure Analysis

  • Kwon, Dae-Young
    • 한국식품위생안전성학회:학술대회논문집
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    • 한국식품위생안전성학회 2003년도 추계 국제심포지움 및 학술발표회
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    • pp.46-46
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    • 2003
  • Hypocholesterolemic peptide isolated from glycimin (11S protein) hydrolyzate by trypsin was purified and identified as LPYP and IAVPGEVA. To investigate the effects of phyiscal properties of side chains of the hypocholesterolemic activity, some of mutant peptides were designed and synthesized chemically. The structure related structures of each peptide were simulated and constructed and their conformations were observed by using spectropolarimeter. The hypocholesterolemic activities were monitored by assaying the inhibition of 3-hydroxy-3-methylglutaryl CoA reductase (HMG-CoA reductase) in vitro and by the determination of cholesterol content in mice serum. For LPYP derivatives, Hypocholesterolemic activity was lost when hydrophobic leucine residue at N-terminus was not so critical for maintaining hypocholesterolemic activity. For idealogical design of hypocholesterolemic peptides, the structure of HMG-CoA reductase are shown and inhibition mechanism of some peptides or inhibitors will be presented. For IAVPGEVA derivative inhibition of HMG-CoA reductase has been studied. For detail study of hypocholesterolemic activity, kinetic study of inhibition of peptides on HMG-CoA reductase and structural view of ligand binding should be investigated.

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Characterization of Extremely Hydrophobic Immunostimulatory Lipoidal Peptides by Matrix Assisted Laser Desorption Ionization Mass Spectrometry

  • 장정석;이성택;장윤석
    • Bulletin of the Korean Chemical Society
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    • 제17권11호
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    • pp.1036-1039
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    • 1996
  • Synthetic lipoidal peptides based on viral protein sequences have been prepared. These peptides contain an N-palmitoyl group at the N-terminal residue, which is a modified cysteine, containing a S-[2,3-bis(acyloxy)-(2-R,S)-propyl] moiety. When this residue (Pam3Cys) is at the N-terminus of a synthetic peptide, it acts as potent immunoadjuvant to enhance both IgM and IgG antibody responses to the attached peptide. Conventional analytical procedures (e.g., Edman degradation and amino acid analysis) are either not applicable due to the N-terminal modification, or do not provide confirmation of the intact structure. Chromatographic analysis is also hindered by the tendency of these lipoidal Pam3Cys peptides to form large aggregates, and in some cases to be permanently adsorbed on reversed phase columns. We have applied several mass spectrometric techniques, including fast atom bombardment (FAB), electrospray ionization (ESI) and matrix assisted laser desorption ionization (MALDI) to characterize the intact structures of a number of different Pam3Cys synthetic peptides. The MALDI-MS has been found to be the most sensitive for the analysis of the structure of Pam3Cys peptides.

Biological activity of peptides purified from fish skin hydrolysates

  • Abuine, Racheal;Rathnayake, Anuruddhika Udayangani;Byun, Hee-Guk
    • Fisheries and Aquatic Sciences
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    • 제22권5호
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    • pp.10.1-10.14
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    • 2019
  • Fish skin waste accounts for part of the solid waste generated from seafood processing. Utilization of fish skin by bioconversion into high-grade products would potentially reduce pollution and economic cost associated with treating fish processing waste. Fish skin is an abundant supply of gelatin and collagen which can be hydrolyzed to produce bioactive peptides of 2-20 amino acid sequences. Bioactivity of peptides purified from fish skin includes a range of activities such as antihypertensive, anti-oxidative, antimicrobial, neuroprotection, antihyperglycemic, and anti-aging. Fish skin acts as a physical barrier and chemical barrier through antimicrobial peptide innate immune action and other functional peptides. Small peptides have been demonstrated to possess biological activities which are based on their amino acid composition and sequence. Fish skin-derived peptides contain a high content of hydrophobic amino acids which contribute to the antioxidant and angiotensin-converting enzyme inhibitory activity. The peptide-specific composition and sequence discussed in this review can be potentially utilized in the development of pharmaceutical and nutraceutical products.

The innate immune response transcription factor Bombyx mori Relish1 induces high-level antimicrobial peptides in silkworm

  • Kim, Seong-Wan;Kim, Seong-Ryul;Goo, Tae-Won;Choi, Kwang-Ho
    • International Journal of Industrial Entomology and Biomaterials
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    • 제37권2호
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    • pp.49-54
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    • 2018
  • To artificially enhance antimicrobial peptide expression in Bombyx mori, we constructed genetically engineered silkworms overexpressing Rel family transcription factor. The truncated BmRelish1 (BmRelish1t) gene contained a Rel homolog domain (RHD), nuclear localization signal (NLS), acidic and hydrophobic amino acid (AHAA)-rich region, and death domain (DD), but no ankyrin-repeat (ANK) domain. The BmRelish1t gene was controlled by B. mori cytoplasmic actin 3 promoter in the PiggyBac transposon vector. Chromosome analysis of G1 generations of a transgenic silkworm with EGFP expression confirmed stable insertion of BmRelish1t. BmRelish1t gene overexpression in transgenic silkworms resulted in higher mRNA expression levels of B. mori antimicrobial peptides such as lebocin(~20.5-fold), moricin(~8.7-fold), and nuecin(~17.4-fold) than those in normal silkworms.

펩티드-물 상호작용. 1-페닐-2-아세트아미도 3-부탄온의 용해도에 대한 압력과 온도의 영향 (Peptide-Water Interaction. Effects of Pressure and Temperature on the Solubility of 1-Phenyl-2-acetamido-3-butanone)

  • 이인자;강상욱;신두순;김건
    • 대한화학회지
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    • 제26권5호
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    • pp.296-303
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    • 1982
  • 수용액 속에서 1-페닐-2-아세트아미도-3-부탄온의 용해도를 20, 30, 40$^{\circ}$C 에서 또 1kg/$cm^2$에서 4500 kg/$cm^2$까지의 압력에서 측정하였다. 압력이 커질수록 용해도는 감소한다. 이러한 용해도 변화는 디케토피페라진과 아주 비슷하다. 디케토피페라진이 펩타이드 결합의 모델 화합물이라는 점과 1-페닐-2-아세토아미도-3-부탄온이 한개의 펩타이드 결합과 상당히 큰 소수성 작용기를 가졌다는 점을 고려할 때 이와 같은 용해도 변화는 예상 밖이다. 부피 변화와 열용량 변화에 대한 값은 이상하다고 할 정도는 아니었다.

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Influence of the N- and C-Terminal Regions of Antimicrobial Peptide Pleurocidin on Antibacterial Activity

  • Cho, Jaeyong;Choi, Hyemin;Lee, Dong Gun
    • Journal of Microbiology and Biotechnology
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    • 제22권10호
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    • pp.1367-1374
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    • 2012
  • Pleurocidin, a 25-mer antimicrobial peptide, has been known to exhibit potent antibacterial activity. To investigate the functional roles in N- and C-terminal regions of pleurocidin on the antibacterial activity, we designed four truncated analogs. The antibacterial susceptibility testing showed that pleurocidin and its analogs exerted antibacterial effect against various bacterial strains and further possessed specific activity patterns corresponding with their hydrophobic scale [pleurocidin > Anal 3 (1-22) > Anal 1 (4-25) > Anal 4 (1-19) > Anal 2 (7-25)]. Fluorescence experiments using 1,6-diphenyl-1,3,5-hexatriene (DPH) and 3,3'-dipropylthiadicarbocyanine iodide [$diSC_3(5)$] indicated that the differences in antibacterial activity of the peptides were caused by its membrane-active mechanisms including membrane disruption and depolarization. Blue shift in tryptophan fluorescence demonstrated that the decrease in net hydrophobicity attenuates the binding affinity of pleurocidin to interact with plasma membrane. Therefore, the present study suggests that hydrophobicity in the N- and C-terminal regions of pleurocidin plays a key role in its antibacterial activity.

Solution Structure of Neuropeptide $\gamma$ from Carassius auratus by NMR spectroscopy

  • Lee, Sangwon;Park, Namgyu;Kim, Yangmee
    • 한국생물물리학회:학술대회논문집
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    • 한국생물물리학회 1999년도 학술발표회 진행표 및 논문초록
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    • pp.31-31
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    • 1999
  • Neuropeptide ${\gamma}$ is a recently identified tachykinin family peptide which has conserved ammo acid sequence of -Phe-X-Gly-Leu-Met-NH2 in the C-terminal region, where X represents aromatic or hydrophobic residues. In this study, three-dimensional structure of neuropeptide ${\gamma}$ from goldfish Carassius auratus (G-NP${\gamma}$) was determined by NMR spectroscopy.(omitted)

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