• Journal of Sericultural and Entomological Science
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• v.50 no.2
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• pp.76-81
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• 2012

Silk protein has been explored to be used for biomedical applications for several decades. However, it has not been used in this field cause to their irreversible crystallization after dissolving in water. The existing methods of silk protein hydrolysis using silkworm cocoon were used with harmful solvents and through a very complicated process. Therefore, we have developed novel methods for the production of water-soluble hydrolysate using silkworm gland. We manufactured two types of silkworm gland-derived hydrolysate (water-soluble SGH, SSGH; total SGH, TSGH) and compared the characteristics with commercial cocoon-derived sericin hydrolysate (CSH). The molecular weight of SGH ranged from 7 to 50 kDa (SSGH) and 5 to 15 kDa (TSGH) within glycine, alanine, and aspartic acid as a main amino acid composition. In contrast, CSH ranged from 15 to 50 kDa within serine and aspartic acid. The results of FTIR implied that SGH was more soluble form than CSH, as shown by the decrease in the ${\beta}$-sheet structure at $1630cm^{-1}$ on amide I peak. In comparison with 10% fetal bovine serum, 0.1% (1 mg/ml) SSGH had equivalent effect on the proliferation of human dermal fibroblasts and mesenchymal stem cells. All results of the SSGH made by novel manufacturing process indicate the SSGH is more preferable as a culture medium supplement than cocoon-derived sericin.

• Food Industry And Nutrition
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• v.4 no.3
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• pp.66-72
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• 1999

• Fisheries and Aquatic Sciences
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• v.5 no.1
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• pp.21-27
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• 2002

Angiotensin I converting enzyme inhibitory activities of shelled krill (Euphausia superba) hydrolysates by autolysis and by hydrolysis with commercial proteases were analyzed. Among the proteases, Alcalase was the most effective protease for the hydrolysis of krill considering the degree of hydrolysis $(87.5\%)$ and the ACE inhibitory activity $(60\%)$. Four hour hydrolysis suggested as the most suitable and economic. In order to establish the optimum hydrolysis condition of krill, degree of hydrolysis and ACE inhibitory activity as affected by Alcalase concentration and water amount added were statistically analyzed by response surface methodology (RSM). The optimum hydrolysis condition was $2.0\%$ Alcalase hydrolysis in 2 volumes (v/w) of water at $55\% for 4 hr. The hydrolysate prepared from the optimum hydrolysis condition was fractionated by molecular weight. The lower molecular weight fraction showed the higher ACE inhibitory activity. $IC_{50}$ of the fraction under 500 Da was 0.57mg protein/mL.

• Journal of Microbiology
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• v.39 no.1
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• pp.17-23
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• 2001

Various pretreatment procedures and selective media were applied to assess the optimal conditions for the isolation of rare actinomycetes from soil. Pretreatment of wet-heating for 15 min at 70$^{\circ}C$ and phenol treatment of soil suspension were the most effective methods for the isolation of these microorganisms. Hair hydrolysate vitamin agar (HHVA) was the most suitable medium for the recovery of rare actinomycetes. Thirty-five rare actinomycete strains were chosen using selective isolation approaches, then morphological and chemical properties of the isolates were determined. The isolates belonged to one of the following genus, Micromonospora, Microbispora, Actinoplanes and Streptosporangium.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.259-262
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• 2003

In this study, batch production of lactic acid from cheap law material such as barley, wheat, and corn, was tried to lower the total production cost of lactic acid. Although no nutrients were supplemented, lactic acid productivities were achieved up to 0.88 $g/(L{\cdot}hr)$ from barley, wheat and com enzymatic hydrolysate. By adding corn steep liquor to com and wheat hydrolysate media, realtively high lactic acid $productivities(4.14\;g/(L{\cdot}hr))$ were obtained.

• Proceedings of the Korean Society of Sericultural Science Conference
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• 2003.10a
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• pp.53-54
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• 2003

The silk fibroin has been widely used as industrial materials such as cosmetics and food l)-2). In this study, general hydrolysate of B. mori fibroin as a biomaterial, development of functional-cometics included hydrolysate of B. mori fibroin will be discussed. (omitted)

• Journal of the Korean Chemical Society
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• v.7 no.1
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• pp.6-12
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• 1963

Amino acid composition of Makjang was determined by combined usage of ion exchanged resin and paper chormatography in the following states. a. A fraction soluble in water b. Hydrolysate of the whole Makjang c. Same as a. (p.p.t. formed by tungstic acid or trichloroacetic acid being removed) d. Hydrolysate of c. (T and TCA) By comparing amino acid composition of Makjang with that of its raw material, we found that decomposition of essential amino acids during brewing is slight. From the amino acid composition of a,b,c,d, we discussed the ratio of amino acid liberation during brewing and assumed that Makjang contains peptide-like substances composing of glutamic acid and aspartic acid.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.12 no.sup1
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• pp.27-36
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• 2009

Some pediatric patients who can not eat orally depend on enteral tube feedings, and some patients require more nutrients and calories to achieve the catch-up growth. If a patient is counting on the parenteral nutrition, early initiation of enteral feeding, orally or enterally, is a very good for the intestinal mucosal maturity and motility. There are numerous kinds of formulas and supplements for the enteral feeding for neonates, infants, and children. Depending on the intestinal symptoms, allergic symptoms, requirement of special nutrients, we can choose regular infant formula (milk-based, soy-based), protein hydrolysate formula, amino acid hydrolysate formula, elemental formula. Proper use of these formulas would help for the pediatric patients to recover from their diseases, to facilitate the intestinal mucosal maturity and to achieve their goal of growth.

• Journal of Microbiology and Biotechnology
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• v.26 no.12
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• pp.2036-2042
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• 2016

We evaluated the potentials of 10 isothiocyanates (ITCs) from cruciferous vegetables and radish root hydrolysate for inhibiting the growth of oral pathogens, with an emphasis on assessing any structure-function relationship. Structural differences in ITCs impacted their antimicrobial activities against oral pathogens differently. The indolyl ITC (indol-3-carbinol) was the most potent inhibitor of the growth of oral pathogens, followed by aromatic ITCs (benzyl ITC (BITC) and phenylethyl ITC (PEITC)) and aliphatic ITCs (erucin, iberin, and sulforaphene). Sulforaphene, which is similar in structure, but has one double bond, showed higher antimicrobial activity than sulforaphane. Erucin, which has a thiol group, showed higher antimicrobial activity than sulforaphane, which has a sulfinyl group. BITC and iberin with a short chain exhibited higher antimicrobial potential than PEITC and sulforaphane with a longer chain, respectively. ITCs have strong antimicrobial activities and may be useful in the prevention and management of dental caries.

• Proceedings of the Korean Society of Grassland Science Conference
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• 1999.06a
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• pp.75.1-75
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• 1999

제초제 저항성 형질전환 목초 생산을 위하여 유전자총(Gene-gun) 및 Agrobacterium 기법을 이용하여 형질전환을 시도한 결과를 요약하면 다음과 같다. 1. 공시재료 : 알팔파 (cv. Vernal, Anker), 오차드그라스 (합성19호) 2. 캘러스의 유도 및 증식 : 알팔파 종자를 SH-3 배지 (SH기본배지, 2,4-D 3mg/L 포함) 그리고 오차드그라스 종자를 MS-5 배지 (MS기본배지, 2,4-D 5mg/L, 2g casein hydrolysate 포함)에 치상하여 캘러스를 유도하였다. 유도된 캘러스는 증식을 위하여 SH-3 및 MS-3 배지 (MS기본배지, 2,4-D 3mg/L, 2g casein hydrolysate 포함)에서 2주 간격으로 subculture 하였다.(중략)