• 대한본초학회지
• /
• 제26권1호
• /
• pp.53-58
• /
• 2011

Objectives : The purpose of this study is to investigate effects of Scutellariae Radix Water Extract on hydrogen peroxide production in RAW 264.7 mouse macrophages. Methods : Scutellariae Radix produced from South Korea (SK) and Scutellariae Radix produced from China (SC) were extracted by hot water. Effects of SK and SC on hydrogen peroxide production in RAW 264.7 were measured by dihydrorhodamine 123 assay after 2, 4, 20, 24, 28, 44, and 48 h incubation at the concentrations of 10, 25, 50, and 100 ug/mL. Results : SK significantly increase hydrogen peroxide production in RAW 264.7 cells for 2, 4, 20, 24, 28, 44, and 48 h incubation at the concentrations of 10, 25, 50, and 100 ug/mL (P < 0.05). SC also significantly increase hydrogen peroxide production in RAW 264.7 cells for 4, 20, 24, 28, and 48 h incubation at the concentrations of 10, 25, 50, and 100 ug/mL (P < 0.05). For 2 h incubation, SC significantly increase hydrogen peroxide production in RAW 264.7 cells at the concentrations of 10, 25, and 100 ug/mL (P < 0.05). For 44 h incubation, SC significantly increase hydrogen peroxide production in RAW 264.7 cells at the concentrations of 10, 25, and 50 ug/mL (P < 0.05). Conclusions : These results suggest that Scutellariae Radix has the immune - enhancing property related with its increasement of hydrogen peroxide production in macrophages.

• 한국환경과학회지
• /
• 제16권12호
• /
• pp.1425-1430
• /
• 2007

This study was conducted to investigate the effect of ozonated water and ozonated water+hydrogen peroxide treatment of residual procymidone in perilla leaf containing 20 mg/L procymidone. Samples was treated with ozonated water containing 1.0, 2.0 and 3.0 mg $O_3/L$ ozone and hydrogen peroxide water containing 1.0, 2.0 and 3.0 mg $H_2O_2/L$ hydrogen peroxide in pH 5, 7 and 9, respectively, at $15^{\circ}C$. Procymidone removal rate was 26.5% in 7 days at $15^{\circ}C$ and optimum condition of procymidone removal was the case of treating with ozonated water containing 2.0 mg $O_3/L$ and pH 9. As the result procymidone removal rate was about 96.5%. In this case of adding hydrogen peroxide, optimum condition of procymidone removal was $1:0.5{\sim}1(O_3:H_2O_2)$. However, procymidone was nearly removed with the treatment of hydrogen peroxide water only.

• 환경위생공학
• /
• 제16권4호
• /
• pp.62-68
• /
• 2001

The advantages of hydrogen peroxide dissolution method were no discharge of noxious matter when dissolution of iron wire which used as the center supporter, reactions occur in room temperature and easy to recover dissolved iron. This study was aimed at gathering the basic data of iron wire dissolution- recovery process and proposes the reaction condition of iron wire dissolution- recovery process rind the factors influencing those reactions. The results were as follows : 1 . Hydrogen peroxide dissolution method used hydrochloric acid as the catalyst. 1. In the dissolution of iron wire(1.668 g), the condition of reaction was E1702(30 ml), HCI(20 ml) and $H_2O$(200 ml) ; time of the reaction was 18 min. P.W.(Piece weight) was 7.75 mg, and C.R. was $2.34{\;}{\Omega}$ 2. In the dissolution of iron wire(1.529 g), the condition of reaction was H7O2(30 ml), HCI(20 ml) and $H_2O$(200 ml), time of the reaction was 21 min., P.W.(Piece weight) was 7.73 mg, and C.R. was $2.35{\;}{\Omega}$. Hydrogen peroxide dissolution method used sulfuric acid as the catalyst. 1. In the dissolution of iron wire(0.834 g), the condition of reaction was $H_2O$(65 ml), $H_2SO_4$(5 ml) and 1702(5 ml) ; time of the reaction was 5 min.30 sec, P.W.(Piece weight) was 7.74 mg, and C.R. was $2.33{\;}{\Omega}$ 2. In the dissolution of iron wire(1.112 g), the condition of reaction was $H_2O$(65 ml), $H_2SO_4$(5 ml) and $H_2O_2$(5 ml) ; time of the reaction was 4 min.30 sec, P.W.(Piece weight) was 7.75 mg, and C.R. was $2.33{\;}{\Omega}$. Hydrogen peroxide dissolution method used hydrochloric acid and sulfuric acid as the catalyst confirmed a clean technology, because there were not occurred a pollutant discharged in the existing method.

• 미생물학회지
• /
• 제31권2호
• /
• pp.166-174
• /
• 1993

Streptomyces coelicolor (Muller) 의 세포에 $100 \mu$M 의 과산화수소를 1시간 동안 처리하여 과산화수소 스트레스중에 생성되는 단백질을 L-[$^{35}S$] methionine 을 이용하여 순간 표지하였다. 단백질을 추출하여 2차원 겔 전기영동으로 분석한 결과 지수 성장기의 세포가 가지는 총세포 단백질 중 약 100개의 단백질의 합성이 과산화수소에 의해 증가되는 것을 관찰하였다. 이들을 Pin(peroxide inducible) 단백질이라고 명명하고 과상화수소 처리 후 발현이 증가되는 시간에 따라 세 그룹으로 나누었다. 약 60 개의 Pin 단백질은 과산화수소 처리후 20분 이내에 발현이 증가하여 1시간동안 지속적으로 다량 합성되었다. 정체 성장기의 세포에서는 62개의 단백질의 합성이 과산화수소에 의해 증가되었으며, 이 중 21 개의 단백질은 지수성장기의 Pin 단백질과 일치하였다. 과산화수소에 대한 저항성이 증가한 세가지 돌연변이체의 단백질을 지수 성장기에서 추출하여 2 차원 겔 전기영동으로 분석한 결과, 각각의 경우 15, 17, 15개의 Pin 단백질을 야생형보다 항상적으로 다량합성하는 것을 관찰하였다. 이 pin 단백질 중 9개 (D74.7 a, E76.0c, E23.3, F50.7, F47.2a, F25.5, G39.6b, G24.0, H39.6a) 는 두가지 돌연변이체 모두에게 증가되었다. 따라서 이 단백질들은 S. coelicolor 가 과산화수소 스트레스에 대응하는데 있어 중요한 역할을 담당한다고 추정된다.

• Bulletin of the Korean Chemical Society
• /
• 제20권6호
• /
• pp.696-700
• /
• 1999

A method has been developed for the determination of trace anion impurities in concentrated hydrogen peroxide. The method involves on-line decomposition of hydrogen peroxide, ion chromatographic separation and subsequent suppressed-type conductivity detection. H2O2 is decomposed in Pt-catalyst filled Gore-Tex membrane tubing and the resulting aqueous solution containing analytes is introduced to the injection valve of an ion chromatograph for periodic determinations. The oxygen gas evolving within the membrane tubing escapes freely through the membrane wall causing no problem in ion chromatographic analysis. Decomposition efficiency is above 99.99% at a flow rate of 0.4mL/min for a 30% hydrogen peroxide concentration. Analytes are quantitatively retained. The analysis results for several brands of commercial hydrogen peroxides are reported.

• 환경위생공학
• /
• 제14권4호
• /
• pp.143-149
• /
• 1999

In the tungsten industry as light source material, tungsten filament which used as light source material could form after molybdenumwire which used as the center supporter for coil shape tungsten wire was removed. This process uses hydrogen peroxide, Ozone and UV(Ultraviolet)Lamp, for the quantity of hydrogen peroxide decrease. The results were as follows : 1. An incandescent electric Lamp type : FL(FL-20) type : A standard of commodity (P.W.: $19{\pm}1.0mg$, $C.R:4.5{\pm}0.3{\Omega}$) 1) Only hydrogen peroxide treated ; Reaction Time : 65Min., P.W.: 18.60mg, $C.R.:4.60{\Omega}$ 2) Ozone/Ultraviolet/70% of hydrogen peroxide; Reaction Time : 64Min., P.W.: 18.61mg, C.R.: $4.61{\Omega}$ 2. A Fluorescent Lamp Type : GLS(GLS-40) Type : A standard of commodity(P.W.: $11.8{\pm}0.2mg$$65{\pm}1.5{\Omega}$) 1) Only hydrogen peroxide treated ; Reaction Time: 72Min, P.W.:11.88mg, C.R.: $65.62\Omega$ 2) Ozone/Ultraviolet/70% of hydrogen peroxide;Reaction Time:71Min., P.W.:11.88mg, C.R.: $65.63\Omega$

• 환경위생공학
• /
• 제14권2호
• /
• pp.1-7
• /
• 1999

In the tungsten industry as light source material, tungsten filament which used as light source material ould form after molybdenum wire which used as the center supporter for coil shape tungsten wire was removed. The purpose of this study is to develop new process named "hydrogen peroxide dissolution method". This process uses hydrogen peroxide, which is harmless to human body and oxidize molybdenum wire selectively. The results were as follows:1. The dissolution of molybdenum wire was proportion to a solution and contact surface of molybdenum wire. 2. The optimum $H_2O_2/H_2O$ were 1:0.5 ~ 1:1.75. 3. The time of dissolution was 65~70 minutes, and the PCS of sample were 20,800 PCS(1,820g). 4. Total cost($H_2O_2$, catalyst) was ￦19,088.

• 자원리싸이클링
• /
• 제10권6호
• /
• pp.9-14
• /
• 2001

폐리튬이온전지의 재활용 일환으로 양극활물질인 $LiCoO_2$로부터 Co와 Li을 회수하기 위하여 황산침출거동을 조사하였다. 황산 2M용액, $75^{\circ}C$ 조건에서 환원제를 첨가하지 않은 경우, $LiCoO_2$로부터 Li가 거의 100% 침출되는 반면에 Co는 같은 조건에서 40% 이하의 침출율을 보였다. Co의 침출율을 향상시키기 위하여 과산화수소를 환원제로 2~20 vol%범위에서 사용하였다 10 vol% 이상의 과산화수소를 첨가하였을 때, Co와 L거 침출율은 모두 95% 이상 이었다. 이는 환원제로서 과산화수소가 Co(III)를 Co(II)로 환원시켜 침출이 용이해졌기 때문으로 생각된다. 황산농도, 온도, 과산화수소 첨가량이 증가함에 따라 Co와 L리 침출율은 증가한 반면에 초기 광액농도가 증가할수록 Co와 L긴 침출율은 감소하였다. 이상의 조업변수 실험을 통하여 코발트계 리튬이온전지의 양극활물질인 $LiCoO_2$의 황산침출에서 황산농도 2 M, 침출온도 $75^{\circ}C$,초기광액농도 100 g/L, 과산화수소 첨가량 20 vol%에서 30분의반응시간이 침출 최적조건으로 생각된다.

• 자원리싸이클링
• /
• 제10권2호
• /
• pp.20-26
• /
• 2001

석유화학 폐촉매의 유가금속을 회수하기 위하여 과산화수소를 환원제로 사용하여 코발트, 망간, 철의 황산침출거동을 조사하였다. 유가금속의 침출은 반응시작 30분 이내에 모두 이루어졌으며, 고액농도 10 g/L, 0.5N $H_2$$SO_4$, $25^{\circ}C$, 30분 300 rpm에서 과산화수소를 0.027mol/L 첨가한 경우가 첨가하지 않은 경우에 비해 망간, 코발트, 철의 침출율이 24.9%, 20.6%, 30.1온에서 93.0%, 87.0%, 100%로 각각 향상되었다 황산농도가 0.5N H2s04까지 증가함에 따라 각 금속들의 침출율은 증가하였으며, 그 이상의 농도에서는 일정한 침출율을 나타내었다 한편, 반응온도가 증가할수록 망간, 코발트, 철의 침출율이 감소하는 경향을 보였다

• 대한한방내과학회지
• /
• 제20권1호
• /
• pp.83-98
• /
• 1999

This study is designed to investigate the effects of Samul-Tang extract on the response of lactic dehydrogenase(LDH) release, cellular activity, lipid peroxidation, DNA synthesis and the changes of total protein of bovine pulmonary artery endothelial cells(PAEC) from hydrogen peroxide$(H_2O_2)$-induced injury. The results are as follows : 1. Samul-Tang significantly decreased $H_2O_2$-induced release of LDH from injured bovine PAEC. 2. Samul-Tang significantly repressed $H_2O_2$-induced cellular activity from injured bovine PAEC. 3. Samul-Tang significantly repressed $H_2O_2$-induced lipid peroxidation from injured bovine PAEC. 4. Samul-Tang significantly stimulated DNA synthesis in bovine PAEC. 5. Samul-Tang significantly repressed $H_2O_2$-induced changes of total protein volume from injured bovine PAEC. Above results suggest that Samul-Tang can protect bovine PAEC from $H_2O_2$-induced injury. These results can be effectively applied to the prevention and cure of cardiovascular and cerebrovascular diseases.