The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
권호 표지

Effects of Samul-Tang Extract on Vascular Endothelial Cells from Hydrogen Peroxide-induced Injury

사물탕(四物湯)이 혈관내피세포(血管內皮細胞)에 미치는 영향(影響)

  • Nam, Chang-Kyu (Dept. of Internal Medicine, College of Oriental Medicine, Se Myutn University) ;
  • Kim, Young-Kyun (Dept. of Internal Medicine, College of Oriental Medicine, Dongeui University) ;
  • Moon, Byung-Soon (Dept. of Internal Medicine, College of Oriental Medicine, Wonkwang University)
  • 남창규 (세명대학교 한의과대학 심계내과학교실) ;
  • 김영균 (동의대학교 한의과대학 심계내과학교실) ;
  • 문병순 (원광대학교 한의과대학 심계내과학교실)
  • Published : 1999.09.29
Download PDF
⟨ Previous Next ⟩

Abstract

This study is designed to investigate the effects of Samul-Tang extract on the response of lactic dehydrogenase(LDH) release, cellular activity, lipid peroxidation, DNA synthesis and the changes of total protein of bovine pulmonary artery endothelial cells(PAEC) from hydrogen peroxide$(H_2O_2)$-induced injury. The results are as follows : 1. Samul-Tang significantly decreased $H_2O_2$-induced release of LDH from injured bovine PAEC. 2. Samul-Tang significantly repressed $H_2O_2$-induced cellular activity from injured bovine PAEC. 3. Samul-Tang significantly repressed $H_2O_2$-induced lipid peroxidation from injured bovine PAEC. 4. Samul-Tang significantly stimulated DNA synthesis in bovine PAEC. 5. Samul-Tang significantly repressed $H_2O_2$-induced changes of total protein volume from injured bovine PAEC. Above results suggest that Samul-Tang can protect bovine PAEC from $H_2O_2$-induced injury. These results can be effectively applied to the prevention and cure of cardiovascular and cerebrovascular diseases.

Keywords

References

  1. 太平惠民和劑局方 陳師文
  2. 儒門事親 張子和
  3. 丹溪心法附餘 v.十九 方廣
  4. 醫學綱目 v.四 樓英
  5. 陳無擇三因方 v.十八 陳言
  6. 經註醫學入門 v.四 李정
  7. 增補萬病回春 공廷賢
  8. 景岳全書 v.下卷 張介賓
  9. 醫方集解 汪訊庵
  10. 醫學心悟(香卷) 程國彭
  11. 醫宗金鑑 吳謙
  12. 血證論 唐容川
  13. 證治彙補 李用粹
  14. 沈氏尊生書 沈金鱉
  15. 東垣十種醫書 李東垣
  16. 徐靈胎醫書全集 v.二 徐靈胎
  17. 醫學正傳 虞天民
  18. 濟陰網目 武之望
  19. 醫宗必讀 李中梓
  20. 東醫寶鑑 許浚
  21. 方藥合編 南山堂編輯局(譯)
  22. 醫門寶鑑 周命新
  23. 漢藥臨床應用 李尙仁;安德均;幸民敎;盧昇鉉;李暎鍾;金先熙
  24. 天眞處方解說 成輔社編輯部(編譯)
  25. 方劑學 v.香淃 上海中醫學院(編)
  26. 方劑學 許濟群;王綿之
  27. 實用中醫方劑學 游士勳;張錦淸
  28. 問答式방제학 申天浩(編譯)
  29. 方劑學 李尙仁;金東傑;李暎鍾;盧昇鉉;朱榮丞
  30. 圖解常用漢方方劑 許鴻源;許照信
  31. 本草學 全國韓醫科大學本草敎授(共編)
  32. 東醫臨床方劑學 尹吉榮
  33. 大韓韓醫學會誌 v.1 no.2 四物湯에 對한 考察 金完熙
  34. 바른 方劑學 康舜洙
  35. 내과학 v.1 해리슨내과학편찬위원회(編)
  36. 병리학 대한병리학회
  37. 조직학 박경아(譯)
  38. 당뇨병학 대한당뇨병학회(編)
  39. 最新診斷과 治療 金東輝(外)
  40. 인체생리학 이정수;노민희;용준환;김덕훈
  41. 成人病·老人病學 徐舜圭
  42. 그림으로 설명한 병리학 이중달
  43. 病理學 金春元
  44. 四物湯 投與가 家兎의 赤血球像에 미치는 影響에 關한 硏究 洪茂昌
  45. 四物湯 構成藥物의 配合이 白鼠의 溶血性 貧血에 미치는 影響 李相弦
  46. 圓光大學校 韓醫科大學 學位論文集 四物湯 煎湯液이 家兎의 血壓降下에 미치는 影響 姜昌洙
  47. 圓光大學校 韓醫科大學 學位論文集 v.2 四物湯이 貧血家兎의 造血效果에 미치는 영향 金世吉
  48. 四物湯의 抗癌劑 副作用 抑制에 關한 實驗的 硏究 安熙悳
  49. 四物湯이 老化白鼠 腦組織의 生化學的 變化에 미치는 影響 朴鍾雲
  50. J. Immune Methods v.65 Rapid colorimetric assay for cellular growth and survival : Application to proliferation and cytotoxic assays Mosmann, T.
  51. Biochem. Med. v.15 A simple fluorometric assay for lipoperoxide in blood plasma Yagi, K.
  52. 金궤要略方論 張仲景
  53. 四物湯 및 季節別 活用方이 血液에 미치는 影響 權在龍
  54. 四物湯과 四物湯의 季節別 倍味 및 加味方이 免疫機能과 血液에 미치는 影響 成昌植
  55. J. Gerontol. v.11 Aging, A theory based on free radical and radiation chemistry Harman, D.
  56. Proc. Natl. Acad. Sci. v.78 The aging process Harman, D.
  57. Science v.201 The biology of oxygen radicals Fridovich, L.
  58. Mech. Age Dev. v.41 Changes in superoxide radical and lipid peroxide formation in the brain, heart and liver during the lifetime of the rat Sawada, M.;Carlson, J.C.
  59. Lab. Invest. v.47 Free radicals and tissue injury Freeman, B.A.;Crapo, J.D.
  60. Am. J. Physiol. v.206 Supplemental fatty acids alter lipid peroxidation and oxidant injury in endothelial cells Hart, C.M.;Tolson, J.K.;block, E.R.
  61. Biochim. Biophys. Acta v.1092 Lipid peroxidation, protein thiol oxidation and DNA damage in hydrogen peroxide-induced injury to endothelial cells, role of activation fo poly (ADP-ribose) polymerase Kirkland, J.B.
  62. J. Surg. Oncol. v.48 Clinical usefulness of chemosensitivity testing using the MTT assay Furukawa, T.;Kubota, T.;Suto, A.;Takahara, T.;Yamaguchi, H.;Takeuchi, T.
  63. Free Radicals in Biology Free radical mechanisms Mead, J.F.;Pryor, W.A.(ed.)
  64. Ann. Rev. Nutr. v.5 Mechanisms and consequences of lipid peroxidation in biological systems Sevanian, A.;Hochstein, P.
  65. Biochim. Biophys. Acta v.961 Lipid peroxidation and phospholipase A activity in libosomes composed of unsaturated phospholipids. A struvtural basis for enzyme activation Sevanian, A.;Wratten, M.L.;Mcleod, L.L.;Kim, E.
  66. Fed. Proc. v.40 Association of lipid peroxidation and polymerization of membrane proteins with erythrocyte aging Hochstein, P.;Jain, S.K.
  67. The Red Cell, Fifth Ann Arbor Conference The physiological significance of oxidative perturbation in erythrocyte membrane lipids and proteins Hochstein, P.;Jain, S.K.;Rice Evans, C.;Brewer, G.J.(ed.)
  68. Lipids v.16 Covalent binding of peroxidized phospholipid to protein. III. Reaction of individual phospholipids with different proteins Nielsen, H.
  69. Gerontology v.37 Protein, lipid and DNA repart systems in oxidative stress, the free-radical theory of aging revisited Pancifici, R.E.;Davies, K.J.A.
  70. Lab. Invest. v.53 Biology of disease, lipid peroxidation and cellular damage in toxic liver injury Comporti, M.
  71. Lipofuscin 1987. State of the Art Protein oxidation, protein cross-linking and proteolysis in the formation of lipofuscin Davies, K.J.A.;Zs Nagy, L.(ed.)
  72. Mutat. Res. v.195 Interaction of lipid peroxidation products with DNA. A review Vaca, C.E.;Wilhelm, J.;Harms-Rinigdahl, M.
  73. Lab. Invest. v.64 Exposure of endothelial cells to free heme potentiates damage mediated by granulocytes and toxic oxygen species Balla, G.;Vercellotti, G.M.;Muller Eberhard, U.;Eaton, J.;Jacob, H.S.
  74. Phytotherapy Research v.7 Protection of vascular endothelial cells from hydrogen peroxide-induced oxidant injury by gypenosides Li, Lin;Benjamin, H.S.L.