• Proceedings of the Korean Vacuum Society Conference
• /
• 2016.02a
• /
• pp.77-77
• /
• 2016

Thin films synthesized by plasma processes have been widely applied in a variety of industrial sectors. The structure control of thin film is one of prime factor in most of these applications. It is well known that the structure of this film is closely associated with plasma parameters and species of plasma which are electrons, ions, radical and neutrals in plasma processes. However the precise control of structure by plasma process is still limited due to inherent complexity, reproducibility and control problems in practical implementation of plasma processing. Therefore the study on the fundamental physical properties that govern the plasmas becomes more crucial for molecular scale control of film structure and corresponding properties for new generation nano scale film materials development and application. The thin films are formed through nucleation and growth stages during thin film depostion. Such stages involve adsorption, surface diffusion, chemical binding and other atomic processes at surfaces. This requires identification, determination and quantification of the surface activity of the species in the plasma. Specifically, the ions and neutrals have kinetic energies ranging from ~ thermal up to tens of eV, which are generated by electron impact of the polyatomic precursor, gas phase reaction, and interactions with the substrate and reactor walls. The present work highlights these aspects for the controlled and low-temperature plasma enhanced chemical vapour disposition (PECVD) of Si-based films like crystalline Si (c-Si), Si-quantum dot, and sputtered crystalline C by the design and control of radicals, plasmas and the deposition energy. Additionally, there is growing demand on the low-temperature deposition process with low hydrogen content by PECVD. The deposition temperature can be reduced significantly by utilizing alternative plasma concepts to lower the reaction activation energy. Evolution in this area continues and has recently produced solutions by increasing the plasma excitation frequency from radio frequency to ultra high frequency (UHF) and in the range of microwave. In this sense, the necessity of dedicated experimental studies, diagnostics and computer modelling of process plasmas to quantify the effect of the unique chemistry and structure of the growing film by radical and plasma control is realized. Different low-temperature PECVD processes using RF, UHF, and RF/UHF hybrid plasmas along with magnetron sputtering plasmas are investigated using numerous diagnostics and film analysis tools. The broad outlook of this work also outlines some of the 'Grand Scientific Challenges' to which significant contributions from plasma nanoscience-related research can be foreseen.

• Proceedings of the Korean Society of Plant Biotechnology Conference
• /
• 2003.04a
• /
• pp.65-71
• /
• 2003

Bax, a mammalian pro-apoptotic member of the Bcl-2 family, induces cell death when expressed in yeast. To investigate whether Bax expression can induce cell death in plant, we produced transgenic Arabidopsis plants that contained murine Bax cDNA under control of a glucocorticoid-inducible promoter. Transgenic plants treated with dexamethasone, a strong synthetic glucocorticoid, induced Bax accumulation and cell death, suggesting that some elements of cell death mechanism by Bax may be conserved among various organisms. Therefore, we developed novel yeast genetic system, and cloned several Plant Bax Inhibitors (PBIs). Here, we report the function of two PBIs in detail. PBI1 is ascorbate peroxidase (sAPX). Fluorescence method of dihydrorho-damine 123 oxidation revealed that expression of Bax in yeast cells generated reactive oxygen species (ROS), and which was greatly reduced by co-expression with sAPX. These results suggest that sAPX inhibits the generation of ROS by Bax, which in turn suppresses Baxinduced cell death in yeast. PBI2 encodes nucleoside diphosphate kinase (NDPK). ROS stress strongly induces the expression of the NDPK2 gene in Arabidopsis thaliana (AtNDPK2). Transgenic plants overexpressing AtNDPK2 have lower levels of ROS than wildtype plants. Mutants lacking AtNDPK2 had higher levels of ROS than wildtype. $H_2O_2$ treatment induced the phosphorylation of two endogenous proteins whose molecular weights suggested they are AtMPK3 and AtMPK6. In the absence of $H_2O_2$ treatment, phosphorylation of these proteins was slightly elevated in plants overexpressing AtNDPK2 but markedly decreased in the AtNDPK2 deletion mutant. Yeast two-hybrid and in vitro protein pull-down assays revealed that AtNDPK2 specifically interacts with AtMPK3 and AtMPK6. Furthermore, AtNDPK2 also enhances the MBP phosphorylation activity of AtMPK3 in vitro. Finally, constitutive overexpression of AtNDPK2 in Arabidopsis plants conferred an enhanced tolerance to multiple environmental stresses that elicit ROS accumulation in situ. Thus, AtNDPK2 appears to play a novel regulatory role in $H_2O_2$-mediated MAPK signaling in plants.

• Journal of Ginseng Research
• /
• v.28 no.4
• /
• pp.191-195
• /
• 2004

An investigation was conducted to ascertain the basic information on characteristics of embryonic tissue development among Korean Ginseng, American ginseng, $F_{1}$ hybrids and $BC_{1}F_{1}(F_{1}$ pollen back cross to $BC_{1}=Korean\;Ginseng)seeds$. The specific activities of lipoxygenase (LOX) and the protein assays were made during the above embry­onic growth period at 5 or 25 degree C. The results are summarized as follows: The fresh weights(mg) of germinating seeds were significantly different among species. Ginseng seeds were stratified at $5^{\circ}C\;for\;60\;days$ the ratios of embryo/endosperms in $P.g.,\;P.q\;or\;F_{1}\;were\;about\;90{\%}$. The ratio was in $BC_{1}F_{1},\;79.2{\%}$ during the same period. The ratios of embryo/endosperms of seeds of P.g. or P.q. germinated at $5^{\circ}C$ showed rather higher values as compared to that at $25^{\circ}C$ LOX specific activity the highest in the $F_{1}$ and decreased in the order of P.g., P.q., and $BC_{1}F_{1}$. The highest LOX specific activity was shown at 80 days after sowing, followed by 70 days sowing, the least LOX activity was shown at 60 days.

• Korean Journal of Environment and Ecology
• /
• v.29 no.5
• /
• pp.651-680
• /
• 2015

In order to investigate vertical plant distribution on Namdeogyu of Mt. Deogyu National Park by temperature change, vascular plants of nine areas with 100-meter-high difference were surveyed from the Deogsangyo (650m alt.) to the Namdeogyusan (1,507m alt.). A total of 455 taxa belonging to 99 families, 280 genera, 402 species, 5 subspecies, 43 varieties, 4 forms and 1hybrid were vegetated on survey areas. Around 700 m high did species diversity of vascular plants decrease rapidly. The Detrended correspondence analysis (DCA) divided distribution of vascular plants into five groups; areas below 700m alt., 700~1,100m alt., 1,100~1,300m alt., and areas above 1,300m alt.. These results showed that vegetation of vascular plants on investigated areas has high correlation with climate elements of temperature. Vascular plants should be crowded within their own optimal ranges of vegetation. Climate change would result in shift of these distribution ranges, and thus vegetation shift will be happened accordingly.

• Korean Journal of Plant Resources
• /
• v.33 no.4
• /
• pp.365-397
• /
• 2020

To investigate the distribution of vascular plants growing at Apsan park, we surveyed from April in 2018 to October in 2019. The flora of Apsan park was classified as a total of 721 taxa comprising of 114 families, 382 genus, 619 species, 5 subspecies, 77 varieties, 12 forma, 5 hybrid, 3 cultivation. Among them, The Rare plants species 23 taxa, endemic plants 15 taxa, floristic regional indicator plants 98 taxa, naturalized plants 57 taxa were recorded. The percentage of urbanization index was 17.76%, the naturalized ratio was 7.91%, respectively.

• Weed & Turfgrass Science
• /
• v.6 no.2
• /
• pp.136-150
• /
• 2017

Zoysiagrass mite, Aceria zoysiae is one of the main turfgrass pests causing significant damage on zoysiagrass. In this study we investigated damage by zoysiagrass mite for the selection of zoysiagrass mite-resistance turfgrass individual in natural collected turfgrass isolates transplanted in genetic resources conservation area. In the results of investigation of 295 turfgrass isolates collected from Korea, damage level was different depending on turfgrass species and individuals. Zoysia japonica was highly damaged representing 97.6%, followed by hybrid zoysia and Z. sinica with damage percentages of 87.7% and 81.1% respectively. Additionally, 2 individuals of Z. macrostachya were also damaged by zoysiagrass mite. However several individuals were not damaged by zoysiagrass mite in green house and field. Damage by zoysiagrass mite was occurred from May and peak at after July in field. The study highlighted some important resistant turfgrass individuals which can be used in the management of zoysiagrass mite.

• Korean Journal of Environmental Biology
• /
• v.32 no.4
• /
• pp.377-381
• /
• 2014

In this study, we report for the first time a hybridization between Caretta caretta and Chelonia mydas in South Korea. On 7th May 2012, a sea turtle (Jeju sea turtle 01, JST01) was found in the east coast of Jeju Island, South Korea. The morphological characteristics of JST01 were compared with those of C. caretta and C. mydas that were frequently observed in Korean Seas. Interestingly, JST01 showed similar morphological characters such as body color, appearance of carapace, shape of upper beak, serration on marginal scute, and two claws with those of C. caretta. On the other hand, JST01 showed also similar patterns in several characteristics including the numbers of marginal, inframarginal and costal scute, smooth carapace without keel, no contact between costal scute and nuchal scute with those of C. mydas. From these results, we suggest that JST01 is one of hybrid animals bred between C. caretta and C. mydas, because JST01 has species-specific characters from both species. We anticipate that our study would be useful and have significant impact onto study inter-specific hybridization in the groups of sea turtle.

• The Plant Pathology Journal
• /
• v.36 no.6
• /
• pp.536-557
• /
• 2020

Sugarcane yellow leaf virus (SCYLV) is a distinct member of the Polerovirus genus of the Luteoviridae family. SCYLV is the major limitation to sugarcane production worldwide and presently occurring in most of the sugarcane growing countries. SCYLV having high genetic diversity within the species and presently ten genotypes are known to occur based on the complete genome sequence information. SCYLV is present in almost all the states of India where sugarcane is grown. Virion comprises of 180 coat protein units and are 24-29 nm in diameter. The genome of SCYLV is a monopartite and comprised of single-stranded (ss) positive-sense (+) linear RNA of about 6 kb in size. Virus genome consists of six open reading frames (ORFs) that are expressed by sub-genomic RNAs. The SCYLV is phloem-limited and transmitted by sugarcane aphid Melanaphis sacchari in a circulative and non-propagative manner. The other aphid species namely, Ceratovacuna lanigera, Rhopalosiphum rufiabdominalis, and R. maidis also been reported to transmit the virus. The virus is not transmitted mechanically, therefore, its transmission by M. sacchari has been studied in different countries. SCYLV has a limited natural host range and mainly infect sugarcane (Sachharum hybrid), grain sorghum (Sorghum bicolor), and Columbus grass (Sorghum almum). Recent insights in the protein-protein interactions of Polerovirus through protein interaction reporter (PIR) technology enable us to understand viral encoded proteins during virus replication, assembly, plant defence mechanism, short and long-distance travel of the virus. This review presents the recent understandings on virus biology, diagnosis, genetic diversity, virus-vector and host-virus interactions and conventional and next generation management approaches.

• Korean Journal of Plant Resources
• /
• v.36 no.1
• /
• pp.32-61
• /
• 2023

To investigate the distribution of vascular plants growing at Wetland in Daegu·Gyeongbook, we surveyed from June 2020 to October in 2021. The flora of wetland was classified as a total of 459 taxa comprising of 104 families, 286 genus, 406 species, 12 subspecies, 34 varieties, 6 forma, 1 hybrid. Among them, the rare plants species 5 taxa, endemic plants 11 taxa, floristic regional indicator plants 57 taxa, naturalized plants 27 taxa and wetland plants 74 taxa were recorded. The percentage of urbanization index was 8.44%, the naturalized ratio was 5.88%, respectively.

• Korean Journal of Environment and Ecology
• /
• v.19 no.4
• /
• pp.348-357
• /
• 2005

The present study was carried out to analyze the leaf variation of hex corn uta which are one of very important native landscape woody materials, but rapidly destroyed by habitat alteration in the past decades. The leaf characters were ranged as 0.9-11.8cm for the leaf length, 0.4-7.7cm for the leaf width, 0.2-13.0mm for the petiole length, $0.7-46.7cm^2$ for the leaf area and 1-13 for the number of leaf serrations. Due to the leaf shape, number of spines, and colours of leaf and fruits, which are the main characters of the cultivars of the Chinese holly, the hollies in the wild and planted as identified as 9 cultivars and one hybrid including 'Cholbuam', 'Inchon' and 'Sun Chun'.