• Title/Summary/Keyword: hybrid species

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Somatic hybridization between Nicotiana rustica and N. tabacum through protoplast fusion (Nicotiana tabacum과 N. rustica 체세포 잡종식물의 육성)

  • Choe, Sang-Ju;Lee, S. C.;Hong, B. H.
    • Journal of the Korean Society of Tobacco Science
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    • v.15 no.2
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    • pp.123-129
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    • 1993
  • Mesophyll protoplasts derived from young leaves of Nicotiana rustica and N. tabacum cv Burley 21 were fused with the aid of polyethylene glycol(PEG). Cytological examination of protoplasts after PEG treatment revealed 12.8 % heterokaryocytes. After 7 weeks culture, the hybrid calli showing greenish white with a compact appearance were selected in contrast to parental type calli tinged with white or green color. The somatic hybrid plants were verified by morphological, biochemical and cyclological analysis. A heterosis effect for plant vigor and height was observed but the shape of leaves and flower characteristics were intermediate between N. tabacum and N. rutstica. The isozyme banding patterns for peroxidase of somatic hybrid lines were compared with the parent species. A number of isozyme bands derived from both parental species were found in the hybrids. Somatic hybrid plants have been successfully backcrossed to the parental N. tabacum particularly with somatic hybrid plants as female parents. These hybrid plants yielded small seeds, only few which were germinable.

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Species and Hybrid Identification of Genus Coreoleuciscus Species in Hwnag-ji Stream, Nakdong River Basin in Korea (낙동강 상류 황지천에 서식하는 쉬리속(genus Coreoleuciscus) 어류 집단의 종 동정 및 잡종 판별)

  • Song, Ha-Yoon;Kim, Jae-Hun;Seo, In-Young;Bang, In-Chul
    • Korean Journal of Ichthyology
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    • v.29 no.1
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    • pp.1-12
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    • 2017
  • A natural hybrid of interspecific between the Coreoleuciscus splendidus and C. aeruginos (Cypriniformes: Cyprinidae) was captured in the Hwang-ji Stream, a tributary of the Nakdong River basin in Korea. An interspecific hybrid between C. splendidus and C. aeruginos was genetically identified based on morphological characteristics and the sequence analysis of nuclear recombination activating gene 1 (RAG1) gene (1,334 bp) and mitochondrial cytochrome c oxidase subunit 1 (CO1) gene (1,551 bp). As a result of morphological variations, the natural hybrid appeared to have an intermediate character between two parental species (C. splendidus and C. aeruginos) in three variations of black array (s) on dorsal, caudal and anal fin rays. Phylogenetic analysis inferred from RAG1 and CO1 sequence data revealed that Coreoleuciscus populations from Hwang-ji stream consist of two pure Coreoleuciscus species and a hybrid individual group. The individuals were clearly identified the cross and reciprocal hybrid by CO1 gene analysis. In RAG1 gene, 13 nucleotide variation loci were detected and the hybrid individuals displayed the double peaks of sequence chromatograms at the 9 diagnostic positions. In this study, molecular data and morphological variations were clearly demonstrated that hybridization did occur between C. splendidus and C. aeruginos. However, F2 hybrid generation and reproductive capacity of F1 hybrid individuals were not demonstrated.

Morphological Characteristics and Molecular Analysis of the Hybrid Takifugu obscurus♀ × T. rubripes♂ (황복(Takifugu obscurus♀)과 자주복(T. rubripes♂) 교잡종의 형태 비교 및 분자분석)

  • Seo-Gyeong Yang;Hyung-Sun Kim;Jin-Lee ;Kyeong-Ho Han
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.56 no.5
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    • pp.708-715
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    • 2023
  • Hybridization is a major production method used to combine beneficial traits from two different species to obtain a potentially dominant trait. In China, Takifugu obscurus and T. rubripes were artificially crossed, and the resulting hybrids had an average body weight 38.06-8.93% higher than that of the parental species, which enabled the hybrids to be grown in freshwater. This study aimed to provide the basic data necessary for the classification of T. obscurus♀×T. rubripes♂ hybrids in terms of economic value and market potential. Morphological comparing the morphology of hybrids and parental species, we discovered that the hybrids had intermediate traits of the parental species. In morphometrics, the hybrid index (HI) value of head length against standard length was close to the trait of T. rubripes, and the HI values of preanal length and predorsal length were close to those of T. obscurus; however, the HI values of nasal length, snout length, length of anal fin, length of pectoral fin, caudal peduncle depth and caudal peduncle length were found to be unique characteristics of the hybrids. Regarding molecular analysis, a 99.8% nucleotide sequence similarity was found between the hybrid and T. obscurus.

Flora of the Coastal Forests on the Same Latitude of East and West Coast in Korea (우리나라 동위도지역(동해안, 서해안) 해안림의 식물상)

  • Kim, Nam-Young;Choi, Hye-Jin;Kim, Young-Seol;Lee, Hak-Bong;Son, Ho-Jun;Park, Wan-Geun
    • Journal of Forest and Environmental Science
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    • v.28 no.2
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    • pp.118-136
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    • 2012
  • Investigation of flora was performed from March to October of 2008 as Eastern and Western coastal districts located in the same latitude ($36^{\circ}47^{\prime}-37^{\circ}48^{\prime}$), in order to apply this to basic data for effective protection and management of coastal forests also for insurance of biodiversity in coastal forests. As a result of the investigation of floras, both coastal forests recorded product-plants as follows: 98 families, 262 genera, 370 species, 46 varieties, 4 forms, 1 hybrid, 421 taxa in total. The Eastern coastal forests had 85 families, 188 genus, 248 species, 32 varieties, 3 forms, 1 hybrid, 285 taxa in total, whereas Western coastal forest had 96 families, 227 genus, 306 species, 39 varieties, 2 forms, 1 hybrid, 348 taxa in total. Among them, seashore plants accounted for 10.2% of total plants appeared in both coastal, and they are 26 families, 38 genus, 40 species, 3 hybrids and 43 taxa in total. There are special Korea plants in both coastal; 9 families, 9 genus, 8 species, 1 varieties, 1 hybrid, 10 taxa in total. There are rare plants in both coastal; 8 families, 8 genus, 7 species, 1 varieties, 8 taxa in total. Naturalized plants are also existed; 11 families, 24 species, 1 variety, 30 taxa in total.

Comparison of Foliar Ultrastructure of 3 Dubautia species (Dubautia속(屬) 3종(種) 식물(植物)의 엽육조직(葉肉組織) 미세구조(微細構造) 비교연구(比較硏究))

  • Kim, In-Sun
    • Applied Microscopy
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    • v.24 no.4
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    • pp.13-31
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    • 1994
  • The fine structure of palisade chloroplasts has been studied in the mature leaves of 3 Dubautia species (D. scabra var. leiophylla, D. knudsenii and D. scabra var. leiophylla${\times}$D. knudsenii) to explore variation at the ultrastructural level, since the parental species exhibit quite different morphological and anatomical features. Types of thylakoidal membrane systems, occurrence and distribution of phytoferritin-like structures, lipid droplets, starch grains, mitochondria and microbodies were examined. Four different types of thylakoidal membranes were found in D. scabra var. leiophylla, 2 rather uniform types in D. knudsenii and 3 intermediate types in their hybrid. D. scabra var. leiophylla and the hybrid were marked by statistically significant differences in mean numbers of thylakoids per granum, while no significant difference was found between D. knudsenii and the hybrid. Phytoferritin-like structures which were about $100-120{\AA}$ in diameter as a whole particle each were found in all 3 species. The amount and distribution of particles varied by species. Lipid droplets, plastoglobuli, and starch grains occurred in all 3 species, but the frequency of starch grains also varied with the species. More frequent and larger starch grains were observed in D. knudsenii than in the other two species. Microbodies, or peroxisome, were observed throughout all species. They occurred, either with or without crystalline inclusions, around the chloroplasts.

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Effect of IOA and Media on Production of Somatic Hybrid by the Asymmetric Protoplast Fusion Between Three Solanum Species

  • Kim Hag-Hyun;Kwon Soo-Jeong;Shin Un-Dong
    • Korean Journal of Plant Resources
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    • v.19 no.3
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    • pp.418-421
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    • 2006
  • In this study, we surveyed the influence of IOA (iodoacetamide) and media upon protoplast fusion for the efficient production of the somatic hybrid among S. sisymbriifolium and other Solanum species (S. intergrifolium and S. toxicarium). Regardless of a breed, as the IOA concentration increases, the cell division tends to decrease. As the influence of the media on the colony formation, we could get 5 colonies from the fusion of S. sisymbriifolium and IOA-treated S. integrifolium protoplast, but none was observed in other fusions. As a result of analyzing their IDH isozyme, we found a somatic hybrid in 2 objects.

Study on the Mechanical Properties of Tropical Hybrid Cross Laminated Timber Using Bamboo Laminated Board as Core Layer

  • GALIH, Nurdiansyah Muhammad;YANG, Seung Min;YU, Seung Min;KANG, Seog Goo
    • Journal of the Korean Wood Science and Technology
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    • v.48 no.2
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    • pp.245-252
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    • 2020
  • This study was performed to analyze the mechanical properties of tropical hybrid cross-laminated timber (CLT) with bamboo laminated board as the core layer in order to evaluate the possibility of its use as a CLT material. Bamboo board was used as the core layer and the tropical species Acacia mangium willd., from Indonesia, was used as the lamination in the outer layer. The modulus of elasticity (MOE), modulus of rupture (MOR), and shear strength of the hybrid CLT were measured according to APA PRG 320-2018 Standard for Performance-Rated Cross-Laminated Timber. The results show that the bending MOE of the hybrid CLT was found to be 2.76 times higher than SPF (Spruce Pine Fir) CLT. The reason why the high MOE value was shown in bamboo board and hybrid CLT applied bamboo board is because of high elasticity of bamboo fiber. However, the shear strength of the hybrid CLT was 0.8 times lower than shear strength of SPF CLT.

Heterologous Expression of Hybrid Type II Polyketide Synthase System in Streptomyces Species

  • Kim, Chang-Young;Park, Hyun-Joo;Kim, Eung-Soo
    • Journal of Microbiology and Biotechnology
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    • v.13 no.5
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    • pp.819-822
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    • 2003
  • Polyketides are an extensive class of secondary metabolites with diverse molecular structures and biological activities. A plasmid-based minimal polyketide synthase (PKS) expression cassette was constructed using a subset of actinorhodin (act) biosynthetic genes (actI-orfl, actI-orf2, actI-orf3, actIII, actⅦ, and actIV) from Streptomyces coelicolor, which specify the construction of an orange-fluorescent anthraquinone product aloesaponarin II, a type II polyketide compound derived from one acetyl coenzyme A and 7 malonyl coenzyme A extender units. This system was designed as an indicator pathway in S. parvulus to generate a hybrid type II polyketide compound via gene-specific replacement. The act ${\beta}-ketoacyl$ synthase unit (actI-orfl and actI-orf2) in the expression cassette was specifically replaced with oxytetracycline ${\beta}-ketoacyl$ synthase otcY-orfl and otcY-orf2). This plasmid-based hybrid PKS cassette generated a novel orange-fluorescent compound structurally different from aloesaponarin II in both S. lividans and S. parvulus. In addition, several additional distinctive blue-fluorescent compounds were detected, when this hybrid PKS cassette was expressed in S. coelicolor B78 (actI-orf2 mutant), implying that the expression of plasmid-based hybrid PKS cassette in Streptomyces species should be an efficient way of generating hybrid type II polyketide compounds.

Ilex × wandoensis C. F. Miller & M. Kim, a new hybrid species of Ilex (Aquifoliaceae) from Korea (감탕나무속(Ilex)의 신잡종, 완도호랑가시나무(I. × wandoensis C. F. Miller & M. Kim))

  • Miller, C.F.;Kim, Muyeol
    • Korean Journal of Plant Taxonomy
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    • v.32 no.3
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    • pp.293-299
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    • 2002
  • A new taxon of Ilex is described from Wando Island, Province Jeonnam, Korea. Ilex x wandoensis C. F. Miller & M. Kim hybrid nov. originates from the natural hybridization of the parent species, I. cornuta Thunb. and I. integra Thunb. Leaves of the new taxon have smooth, spinose margins, a condition that is intermediate between the twisted, spinose leaf margins of I. cornuta and the smooth, entire margins of I. integra. The new hybrid species shares several characters with its two parents, including evergreen leaves, dioecy, and red drupes.

The concept of hybride phenomean is to selected to describe the fashiontrend of ‘98-00’. (‘98-00’ Fashion Trend에 나타난 Hybrid 현상에 관한 고찰)

  • 정미혜
    • Journal of the Korea Fashion and Costume Design Association
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    • v.1 no.1
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    • pp.5-16
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    • 1999
  • The purpose of this study is to identify the distinctive characterics HYBRID, strictly defined, an offspring that is a cross between different species, genera, or, in rare cases, families, More loosely defined, a hybrid can also be a cross between parents of different subspecies or varieties of a species. Hybrid varieties of plants and animals are of economic value because the hybrid is nearly always more vigorous, larger, and more fertile than either of its parents. Plant and animal hybrids may arise spontaneously in nature or may be produced intentionally by man. Forward-looking fashion explores progress. Adjusting to a world that has integrated genetics and its mutations, the new silhouette seems without references, as if born from a spontaneous generation. A slightly bizarre composite, asymmetrical, discreetly technological aesthetic, it draws on the latest technological details. the pursuit of performance and ergonomics, yet also of comfort and amusement that takes into account the body and its articulations, generates strange garments. Inspired by medical protheses. Focos on fabric touches, their weightiness and surface effects obtained with silicone, rubber or wax. in a range that highlights yellows but can also contrast with colder, slightly surgical hues, the hybrid woman doesn't necessarily want to stand out.

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