Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Effect of IOA and Media on Production of Somatic Hybrid by the Asymmetric Protoplast Fusion Between Three Solanum Species

  • Kim Hag-Hyun (Dept. of Urban Horticulture & Landscape Architecture, Woosong Information College) ;
  • Kwon Soo-Jeong (Dept. of Urban Horticulture & Landscape Architecture, Woosong Information College) ;
  • Shin Un-Dong (Dept. of Urban Horticulture & Landscape Architecture, Woosong Information College)
  • Published : 2006.06.01
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Abstract

In this study, we surveyed the influence of IOA (iodoacetamide) and media upon protoplast fusion for the efficient production of the somatic hybrid among S. sisymbriifolium and other Solanum species (S. intergrifolium and S. toxicarium). Regardless of a breed, as the IOA concentration increases, the cell division tends to decrease. As the influence of the media on the colony formation, we could get 5 colonies from the fusion of S. sisymbriifolium and IOA-treated S. integrifolium protoplast, but none was observed in other fusions. As a result of analyzing their IDH isozyme, we found a somatic hybrid in 2 objects.

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References

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