• Journal of Plant Biotechnology
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• v.49 no.4
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• pp.331-338
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• 2022

Sunflower (Helianthus annuus L.) protoplasts were isolated from seven-day-old etiolated hypocotyls of 10 A line and four-week-old fully expanded young leaves of PI 441983 line in vitro seedlings using an enzymatic method. Purified protoplasts were collected by filtration and floatation in sucrose solution. Semi-solid protoplast culture was performed using the L4 regeneration protocol with various culture media and plating densities to achieve the highest efficiencies for protoplast culture of hypocotyl and mesophyll protoplasts of 10 A and PI 441983 lines, respectively. The concentrations in liquid L'4M medium and different plating densities were evaluated in two types of cytokinins, the adenine-type 6-benzyladenine (BA) and the phenylurea-type thidiazuron (TDZ). The highest colony formation was achieved in both sunflower lines when 0.5 mgL^-1 BA and 0.5 mgL^-1 TDZ were applied with a high plating density (3 × 10⁵ protoplasts mL^-1). These conditions led to 38.45% and 39.40% colony formation for hypocotyl protoplasts of the 10 A line and mesophyll protoplasts of the PI 441983 line, respectively. Moreover, many hypocotyl protoplast-derived colonies developed into micro-calli. In addition, superior development of both sunflower protoplasts was observed with all plating densities when BA was used in combination with TDZ. This finding will be applicable to future sunflower hybrid production via somatic hybridization.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.16 no.2
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• pp.97-103
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• 2023

Since most of the information is transmitted through the network, eavesdropping and interception by a third party may occur. Appropriate measures are required for effective, secure and confidential communication in the network. Steganography is a technology that prevents third parties from detecting that confidential information is hidden in other media. Due to structural vulnerabilities, information protected by encryption and steganography techniques can be easily exposed to illegitimate groups. In order to improve the limitations of LSB where the simplicity and predictability of the hiding method exist, I propose a technique to improve the security of the message to be hidden based on PRNG and recursive function. To enhance security and confusion, XOR operation was performed on the result of selecting a random bit from the upper bits of the selected channel and the information transformed by the RS-box. PSNR and SSIM were used to confirm the performance of the proposed method. Compared to the reference values, the SSIM and PSNR of the proposed method were 0.9999 and 51.366, respectively, confirming that they were appropriate for hiding information.

• Current Research on Agriculture and Life Sciences
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• v.1
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• pp.1-9
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• 1983

This study was conducted to obtain basic information on the rice anther culture. Materials used were (Inabawase X YR 2404-14-2-1) $F_1$ hybrid. Callus growth rate on various media, induction frequency of callus in spikelet and panicle, and the effect of treatment on anther and callus were evaluated. The results obtained were summarized as follows ; The growth rate of callus on N-6, M-S, P.E.agar media was 19.8, 13.1, 4.1 times respectively after 30 days inoculated, and on liquid media was 3.8, 5.1, 1.4 times, respectively. Organ differentiation on N-6, M-S, P.E.agar media was 37.5%, 12.5%, 17.5% respectively. The difference of induction frequency of callus per panicle was 0.14%-6.25% and per spikelet was 0-19.05%. Almost callus was induced 30-35 days after inoculation. Organ differentiation of induced callus was decreased by culture. Callus cultured for 13 days after induction did not make shoot. Anthers cold shocked at $8^{\circ}C$ for 5 days obtained 3.32% efficiencys of callus induction per number of anthers plated, and compared with 2.41% of no treated anthers. But anther treated at $8^{\circ}C$ for 7 days decreased 2.24%. Callus induction periods were shortened by cold treatment for about 5 days. Callus cultured on medium containing 2 mg/l of 2, 4-D showed 5% on root formation but medium containing 5 mg/l of 2, 4-D showed 30% of root formation after transfered on the medium without 2, 4-D. Callus cold shocked at $15-18^{\circ}C$ revealed poor efficiency for root formation, but 5 days treatment was good for shoot formation.

• Cartoon and Animation Studies
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• s.37
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• pp.509-537
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• 2014

This paper proposes 'postfilimc metamorphosis' and 'reanimation' as two concepts that aim at giving account to the aesthtetic tendencies and genealogies of what Suzanne Buchan calls 'pervasive animation', a category that refers to the unprecedented expansion of animation's formal, technological and experiential boundaries. Buchan's term calls for an interdisciplinary approach to animation by highlighting a range of phenomena that signal the growing embracement of the images and media that transcend the traditional definition of animation, including the lens-based live-action image as the longstanding counterpart of the animation image, and the increasing uses of computer-generated imagery, and the ubiquity of various animated images dispersed across other media and platforms outside the movie theatre. While Buchan's view suggests the impacts of digital technology as a determining factor for opening this interdisciplinary, hybrid fields of 'pervasive animation', I elaborate upon the two concepts in order to argue that the various forms of metamorphorsis and motion found in these fields have their historical roots. That is, 'postfilmic metamorphosis' means that the transformative image in postfimic media such as video and the computer differs from that in traditional celluloid-based animation materially and technically, which demands a refashioned investigation into the history of the 'image-processing' video art which was categorized as experimental animation but largely marginalized. Likewise, 'reanimation' cne be defined as animating the still images (the photographic and the painterly images) or suspending the originally inscribed movement in the moving image and endowing it with a neewly created movement, and both technical procedues, developed in experimental filmmaking and now enabled by a variety of moving image installations in contemporary art, aim at reconsidering the borders between stillness and movement, and between film and photography. By discussing a group of contemporary moving image artworks (including those by Takeshi Murata, David Claerbout, and Ken Jacobs) that present the aesthetic features of 'postfilmic metamorphosis' and 'reanimation' in relation to their precursors, this paper argues that the aesthetic implications of the works that pertain to 'pervasive animation' lie in their challenging the tradition dichotomies of the graphic/the live-action images and stillness/movement. The two concepts, then, respond to a revisionist approach to reconfigure the history and ontology of other media images outside the traditional boundaries of animation as a way of offering a refasioned understanding of 'pervasive animation'.

• Journal of Broadcast Engineering
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• v.17 no.4
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• pp.640-658
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• 2012

One of the existing terrestrial multi-channel DTV service frameworks, called KoreaView, provides four programs, composed of MPEG-2 based one HD video and H.264/AVC based three SD videos within one single 6MHz frequency bandwidth. However the additional 3 SD videos can not provide enough quality due to its reduced spatial resolution and low target bitrates. In this paper, we propose a framework, which is called a terrestrial multi-channel high quality hybrid DTV service, to overcome such a weakness of KoreaView services. In the proposed framework, the three additional SD videos are encoded based on an H.264/SVC Spatial Base layer, which is compliant with H.264/AVC, and are delivered via broadcasting networks. On the other hand, and the corresponding three additional HD videos are encoded based on an H.264/SVC Spatial Enhancement layer, which are transmitted over broadband networks such as Internet, thus allowing the three additional videos for users with better quality of experience. In order to verify the effectiveness of the proposed framework, various experimental results are provided for real video contents being used for DTV services. First, the experimental results show that, when the SD sequences are encoded by the H.264/SVC Spatial Base layer at a target bitrate of 1.5Mbps, the resulting PSNR values are ranged from 34.5dB to 42.9dB, which is a sufficient level of service quality. Also it is noted that 690kbps-8,200kbps are needed for the HD test sequences when they are encoded in the H.264/SVC Spatial Enhancement layer at similar PSNR values for the same HD sequences encoded by MPEG-2 at a target bitrate of 12 Mbps.

• The Journal of Natural Sciences
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• v.4
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• pp.103-142
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• 1991

These studies were carried out to select somatic hybrid using selectable marker genes of Nicotiana glauca transformed by NPTII gene and Solanum tuberosum transformed by T- DNA, and to study characteristics of transformant. The results are summarized as follows. 1. Crown gall tumors and hairy roots were formed on potato tuber disc infected by A. tumefaciens Ach5 and A. rhizogenes ATCC15834. These tumors and roots could be grown on the phytohormone free media. 2. Callus formation from hairy root was prompted on the medium containing 2, 4 D 2mg/I with casein hydrolysate lg/l. 3. The survival ratio of crown gall tumor callus derived from potato increased on the medium containing the activated charcoal 0. 5-2. 0mg/I because of the preventions on the other hand, hairy roots were necrosis on the same medium. 4. Callus derived from hairy root were excellently grown for a short time by suspension culture on liquid medium containing 2, 4-D 2mg/I and casein hydrolysate lg/l. 5. The binary vector pGA643 was mobilized from E. coli MC1000 into wild type Agrobacteriurn tumefaciens Ach5, A. tumefaciens $A_4T$ and disarmed A. tuniefaciens LBA4404 using a triparental mating method with E. ccli HB1O1/pRK2013. Transconjugants were obtained on the minimal media containing tetracycline and kanamycin. pGA643 vectors were confirmed by electrophoresis on 0.7% agarose gel. 6. Kanamycin resistant calli were selected on the media supplemented with 2, 4-D 0.5mg/1 and kanamycin $100\mug$/ml after co- cultivating with tobacco stem explants and A. tumefaciens LBA4404/pGA643, and selected calli propagated on the same medium. 7. The multiple shoots were regenerated from kanamycin resistant calli on the MS medium containing BA 2mg/l. 8. Leaf segments of transformed shoot were able to grow vigorusly on the medium supplemented with high concentration of kanamycin $1000\mug$/ml. 9. Kanamycin resistant shoots were rooting and elongated on medium containing kanamycin $100\mug$/ml, but normal shoot were not. 10. For the production of protoplast from potato calli transformed by T-DNA and mesophyll tissue transformed by NPTII gene, the former was isolated in the enzyme mixture of 2.0% celluase Onozuka R-10, 1.0% dricelase, 1.0% macerozyme. and 0.5M mannitol, the latter was isolated in the enzyme mixture 1.0% Celluase Onozuka R-10, 0.3% macerozyme, and 0.7M mannitol. 11. The optimal concentrationn of mannitol in the enzyme mixture for high protoplast yield was 0.8M at both transformed tobacco mesophyll and potato callus. The viabilities of protoplast were shown above 90%, respectively. 12. Both tobacco mesophyll and potato callus protoplasts were fused by using PEG solution. Cell walls were regenerated on hormone free media supplemented with kanamycin after 5 days, and colonies were observed after 4 weeks culture.

• Journal of Broadcast Engineering
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• v.22 no.4
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• pp.496-508
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• 2017

In this paper, we propose a Tone Mapping Operator (TMO) that preserves global contrast and precisely preserves boundary information. In order to reconstruct a High Dynamic Range (HDR) image to a Low Dynamic Range (LDR) display by using Threshold value vs. Intensity value (TVI) based on Human Visual System (HVS) and contrast value. As a result, the global contrast of the image can be preserved. In addition, by combining the boundary information detected using Guided Image Filtering (GIF) and the detected boundary information using the spatial masking of the Just Noticeable Difference (JND) model, And improved the perceived image quality of the output image. The conventional TMOs are classified into Global Tone Mapping (GTM) and Local Tone Mapping (LTM). GTM preserves global contrast, has the advantages of simple implementation and fast execution time, but it has a disadvantage in that the boundary information of the image is lost and the regional contrast is not preserved. On the other hand, the LTM preserves the local contrast and boundary information of the image well, but some areas are expressed unnatural like the occurrence of the halo artifact phenomenon in the boundary region, and the calculation complexity is higher than that of GTM. In this paper, we propose TMO which preserves global contrast and combines the merits of GTM and LTM to preserve boundary information of images. Experimental results show that the proposed tone mapping technique has superior performance in terms of cognitive quality.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.16 no.6
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• pp.65-73
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• 2016

Peer-to-Peer (P2P) systems with super-peer overlay networks show combined advantages of both hybrid and pure P2P systems. Super-peer is a special peer acting as a server to a cluster of generic peers. Organizing a super-peer network is one of important issues for P2P systems with super-peer networks. Conventional P2P systems are based on two-level hierarchies of peers. One is a layer for generic peers and the other is for super-peers. And it is usual that super-peer networks have forms of random graphs. However, for accommodating a large-scale collection of generic peers, the super-peer network has also to be extended. In this paper, we propose a scheme of hierarchically constructing super-peer networks for large-scale P2P systems. At first, a two-level tree, called a simple super-peer network, is proposed, and then a scheme of generalizing and then extending the simple super-peer network to multi-level super-peer network is presented to construct a large-scale super-peer network. We call it an extended super-peer network. The simple super-peer network has several good features, but due to the fixed number of levels, it may have a scalability problem. Thus, it is extended to k-level tree of a super-peer network, called extended super-peer network. It shows good scalability and easy management of generic peers for large scale P2P system.

• Proceedings of the Korea Contents Association Conference
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• 2006.11a
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• pp.223-226
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• 2006

It can formulate a basic concept to the study on styles of drawing on animation which is influenced by styles of drawing on comic. Especially the transferability of comic and eel animation have high possibility because they have common part as drawing, continuity of imagery and narrative, in spit of the differentiation with comic for spatial and animation for temporal. So the study on comic drawing styles are inevitable check point for investigating it's animation. To analyse of the style of animation drawing, can be employed the styles of comic drawing. For a case study, comic authors and their works is utilized for establishing basic concept of transferring comic to animation with the influence of drawing styles to animation and than it will apply to classify and analyse animation by them. The consequence of this study, it can be sorted in the three areas that are the cheerful comic, the drama, the boy-meets-girl comic. and can be proved that the close relationship of comic and animation. Among three aspects, a boy-meets-girl comic can not be detected in any selected animations. The presumption of this result that can be apply to the general idea which is a rare case to use in animation. Also to use partly cheerful comic to drama style as different drawing styles is for elucidating the effect of intending isolation with the play theory of Bertolt Brecht and empathizing dramatical situation through the disparity of emotion. This study is to investigate on the influence of the drawing style of comics in animation, and can provide the basic ideas of inter-relation of media through drawing styles study with the concept of hybrid.

• Journal of Broadcast Engineering
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• v.9 no.1
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• pp.17-31
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• 2004

This paper proposes an algorithm for the robust transmission of video in error Prone environment using multiple description codingby optimal split of DCT coefficients and rate-distortionoptimization framework. In MDC, a source signal is split Into several coded streams, which is called descriptions, and each description is transmitted to the decoder through different channel. Between descriptions, structured correlations are introduced at the encoder, and the decoder exploits this correlation to reconstruct the original signal even if some descriptions are missing. It has been shown that the MDC is more resilient than the singe description coding(SDC) against severe packet loss ratecondition. But the excessive redundancy in MDC, i.e., the correlation between the descriptions, degrades the RD performance under low PLR condition. To overcome this Problem of MDC, we propose a hybrid MDC method that controls the SDC/MDC switching according to channel condition. For example, the SDC is used for coding efficiency at low PLR condition and the MDC is used for the error resilience at high PLR condition. To control the SDC/MDC switching in the optimal way, RD optimization framework are used. Lagrange optimization technique minimizes the RD-based cost function, D+M, where R is the actually coded bit rate and D is the estimated distortion. The recursive optimal pet-pixel estimatetechnique is adopted to estimate accurate the decoder distortion. Experimental results show that the proposed optimal split of DCT coefficients and SD/MD switching algorithm is more effective than the conventional MU algorithms in low PLR conditions as well as In high PLR condition.