• 한국유기농업학회지
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• 제23권3호
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• pp.497-507
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• 2015

내병성 포도 신품종을 육성하기 위해서는 병해 저항성인 유전자원을 선발하는 것이 요구된다. 본 연구에서는 머스카딘포도와 플로리다 교잡종포도를 대상으로 병원균을 상처접종하여 포도나무 줄기혹병에 대한 저항성을 검정하였다. 3종류의 Agrobacterium vitis 균주를 접종한 포도나무는 품종 간에는 혹의 형성에 차이를 나타내었지만, 병원균의 균주 간에는 차이가 나타나지 않았다. 머스카딘포도는 많은 품종이 감수성이었으며, 'Gold Isle'과 'Africa Queen'은 고도의 감수성이었고 'Welder'와 'Jumbo'는 저항성이었다. 플로리다 교잡종포도는 머스카딘포도에 비해 덜 민감하였으며 'Daytona'와 'Stover' 등은 감수성이고, 'Blanc du Bois'는 중도감수성이었다. 본 연구는 머스카딘포도를 대상으로 줄기혹병에 대한 품종저항성을 판별함으로써 아속간 교잡을 통한 줄기혹병 저항성 포도 신품종육성에 중요한 정보를 제공할 것이다.

• 한국수산과학회지
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• 제55권6호
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• pp.903-909
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• 2022

Triploid fish have attracted the attention of fish farmers owing to of their larger cell size compared to that of diploid fish and their ability to utilize the energy required for sexual maturation for growth instead. However, the effect of increased growth in triploid fish has shown varying results. Therefore, this study aimed to compare growth between diploid and triploid starry flounders and diploid and triploid hybrid juveniles (starry flounder, Platichthys stellatus ♀×stone flounder, Kareius bicoloratus ♂) based on breeding water temperature to investigate the effect of increased growth in triploid fishes. The experiment was performed by categorizing the water temperature as high temperature (EXP. 1, 14.5-23.5℃) and low temperature (EXP. 2, 6.4-13.3℃) based on the optimal water temperature for starry flounder breeding. In EXP. 1, the growth of starry flounder triploids and hybrid triploids was significantly higher than that of diploids. However, in EXP. 2 the weight gain and specific growth rate of the starry flounder triploid were higher, and the growth of starry flounder was higher than that of the hybrids.

• Asian-Australasian Journal of Animal Sciences
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• 제18권9호
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• pp.1237-1241
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• 2005

Mannose-P-dolichol utilization defect 1 (MPDU1) gene is required for utilization of the mannose donor MPD in synthesis of both lipid-linked oligosaccharides (LLOs) and glycosylphosphatidylinositols (GPI) which are important for functions such as protein folding and membrane anchoring. The full length cDNA of the porcine MPDU1 was determined by in silico cloning and rapid amplification of cDNA ends (RACE). The deduced amino acid showed 91% identity to the corresponding human sequence with five predicted transmembrane regions. RT-PCR was performed to detect its expression pattern in five tissues and results showed that it is expressed ubiquitously among the tissues checked. A single nucleotide substitution resulting in the amino acid change (137 Tyr-137 His) was detected within exon 5. Allele frequencies in six pig breeds showed distinctive differences between those Chinese indigenous pigs breeds and European pigs. Using the pig/rodent somatic cell hybrid panel (SCHP), we mapped the porcine MPDU1 gene to SSC12, which is consistent with the comparative mapping result as conservative syntenic groups presented between human chromosome 17 and pig chromosome 12.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1993년도 추계 학술대회지
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• pp.24-25
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• 1993

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2000년도 제7차 국제 심포지움(생약자원개발에 관한연구) 및 추계정기 학술발표회 초록집
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• pp.29-29
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• 2000

• 한국작물학회:학술대회논문집
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• 한국작물학회 2004년도 춘계 학술대회지
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• pp.84-85
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• 2004

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2002년도 Join Meetings of Korean Society of Sericultural Science and Japanse Society of Sericultural Science
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• pp.33-33
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• 2002

No Abstract, See Full Text

• Journal of Plant Biotechnology
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• 제45권3호
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• pp.236-241
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• 2018

Lilium is a perennial bulbous plant belonging to the liriotypes genus. Our aim was to study the phylogenetic relationships of the Lilium family. Two varieties of Lilium ledebourii, 44 varieties of the gene bank, and one variety from the Tulipa family served as the out group. In order to study the diversity between lilium masses, ITS regions were used to design the marker. The results showed that the guanine base is the most abundant nucleotide. Relatively high conservation was observed in the ITS regions of the populations (0.653). Phylogenetic analysis showed that sargentiae and hybrid varieties are older than other varieties of the Lilium family. Also, the location of L. ledebourii varieties (Damash and Namin) was identified in a phylogenetic tree by using the ITS marker. Overall, our research showed that ITS molecular markers are very suitable for phylogenetic studies in the Lilium family.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.61-61
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• 2019

In any crop breeding program Selection and use of genetically diverse genotypes to develop cultivars with a broad genetic base is important. Molecular markers play a major role in selecting diverse genotypes. Molecular breeding programs of the crop can be made more efficient by use of molecular markers. The present study was done with an aim of analyzing genetic diversity and the population structure in 24 accessions of sunflower (Helianthus annus L.) from Kenya genetic diversity using 35 EST-SSR and gSSR primers.Out of the 35 markers 3 were not polymorphic as they indicated Polymorphic Information content( PIC) of value 0.00 and so the data analysis was done using 32 markers . The 32 set of markers used produced 29 alleles ranging from 2 to 7with a mean of 3.0 alleles per locus.The average value of polymorphic information contents(PIC) were 0.3 .Genetic diversity analysis using these markers revealed 3 major clusters. This result could be useful for designing strategies to make elite hybrid and inbreeding of crossing block for breeding and future molecular breeding programs to make elite variety.

• 한국작물학회지
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• 제30권4호
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• pp.431-435
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• 1985

우리나라 수도 품종을 배경으로 한 세포질적 유전적 웅성불임계통들에 우리나라 품종의 임성회복친을 교잡시킨 HR1619A/남풍벼, HR1619A/가야벼, HR1619A/계통234, 통일A/남풍벼, 통일A/청청벼, 수원296A/계통209, 수원296A/계통237, 수원296A/계통252 및 계통201A/계통234 등 9개 Hybrid를 육성하고 이들의 수량 및 수량 구성요소, 흰빛잎마름병 저항성 및 미질을 조사하여 다음과 같은 결과를 얻었다. 1 공시된 9개 Hybrid들 중 계통201A/계통234, 통일A/남풍벼 및 HR 1619A/남풍벼 등 3개 Hybrid의 수량성이 높았는데 각각 우수친에 비해 36％, 17％ 및 10％의 증수(Heterobeltiosis)를, 표준품종인 청청벼에 비해 각각 19％, 17％ 및 14％의 증수(Standard Heterosis)를 보였다. 2. 공시된 Hybrid중 HR1619A/가야벼와 수원296A/계통237의 수량은 양친보다 낮았다. 3. 수량구성요소 중 수당입수의 잡종강세가 현저하였고 주당수수, 천립중 및 임실율의 잡종강세는 인정되지 않았다. 4. Hybrid의 흰빛잎마름병 저항성은 양친 중 강한친과 같은 반응을 보였다. 5. Hybrid들의 Amylose 함량은 20.8％∼24.7%였고 딘백질함량은 9.3％∼10.0％, 알칼리 붕괴도는 3.7∼7.0으로서 각각 양친품종과 비슷하였다.